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The map label for this gene is prpD [H]

Identifier: 126446033

GI number: 126446033

Start: 444304

End: 445755

Strand: Reverse

Name: prpD [H]

Synonym: BMA10247_A0494

Alternate gene names: 126446033

Gene position: 445755-444304 (Counterclockwise)

Preceding gene: 126447450

Following gene: 126446719

Centisome position: 18.95

GC content: 68.11

Gene sequence:

>1452_bases
ATGTCCGCCCCTATCCCGAACGTCCGGCCAGCGCCGGATTCAGTGCTGGTCGAGATCGCCGACTACGCGCTGAACCATCG
CATCGACAGTGCGCTCGCGCTCGAAACCGCGCGCCACTGCCTGATCGATACGCTCGGGTGCGGGCTCGAGGCGCTGTCGT
ATCCCGCCTGCACCAAGCTGCTCGGGCCGATCGTGCCCGGCACGATCGTGCCGCACGGCGCGAAGGTGCCGGGCACGTCG
CTGCAGCTCGACCCGGTGCAGGCCGCGTTCAACATCGGCGCGGCGATCCGCTGGCTCGACTTCAACGACACGTGGCTCGC
GGCCGAATGGGGCCATCCTTCGGACAACCTGGGCGGCATCCTCGCGACGGCCGACTGGCTCTCGCGCACCGCCGTCGCGT
CCGGCAAGCGGCCGCTCGCGATGAAGGACGTGCTCGTCGCGATGATCCATGCGCATGAGATCCAGGGCTGCCTCGCGCTC
GAAAATTCGTTCAATGCGGTCGGCCTCGATCACGTGCTGCTCGTGAAGGTCGCCTCGACCGCGACCGTCGGCTGCCTGCT
CGGCCTCACGCGCGACGAGCTGATCAACGCGCTGTCGCTCGCGTTCGTCGACGGCCAGGCGCTGCGCACCTATCGCCACG
CGCCGAACACCGGCTCGCGCAAGTCGTGGGCGGCGGGCGACGCGACCTCGCGCGCGGTGCGCCTCGCGCTGATCGCGAAA
ACGGGCGAGATGGGCTACCCGCGCGCGCTCACCGCGAAAACCTGGGGCTTCTACGACGTGTCGTTCGGCGGCAAGCCGTT
CCGCTTCCAGCGCCCATACGGCACCTACGTGATGGAGAACGTGCTGTTCAAGCTCTCGTATCCGGCCGAATTCCACGCGC
AGACGGCCGTCGAAGCCGCGATGACGCTGCACGCGCGGCTCGCGGCGGCCGGGCGCACCGCCGACGAGATCCGCCGCGTC
ACCGTGCGCACGCATGCGGCCGCGATGCGCATCATCGACAAGCAAGGGCCGCTCGCGAACCCGGCCGACCGCGATCACTG
CATCCAGTACATGATCGCCGTGCCGCTCCTGTTCGGCCGCCTCACGGCCGCCGACTACGAGGACGCGGTGGCGGCCGATC
CGCGGATCGACGCGCTGCGCGCGAAGATCGCGTGCGTCGAGGACCCGCAGTTCACGCAGGATTATCACGACCCGGCGAAG
CGCTCGATCGCCAATGCGCTCACGATCGAGTTCGACGACGGATCGACGCTCGCCGAGGTGGCGGTCGAATACCCGATCGG
CCACATGCGCCGGCGCGCCGAAGGGATGGCGCTCCTGATCGACAAGTTCAGGACGAATCTCGCGCGCCGCTTTCCGGCCA
AGCAGCAACAAGCGATTCTCGACGTGTCGCTGGACCAGGCAAAGCTCGAAGCGATGCCGGTCGATGAGTACGTCGATCTG
TATGTGATCTAG

Upstream 100 bases:

>100_bases
AACGAGCTGAAGCCGTCGAACTGAGGCCGCCCGACAGCCACCTTTGCGCATGAGACCGGAGCCGGCGCCGCCGCGCCCGA
GCCGGTCGACAGGAGCCTCG

Downstream 100 bases:

>100_bases
CCGTCAAATACAGAGTTCTTTCCTAGCCTTAAACCCAGGAAATCACCATGGCCCACAATCTCCACAAAACACTCAAGGAA
TTCGACAGCGGTTCCGGCAA

Product: 2-methylcitrate dehydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 483; Mature: 482

Protein sequence:

>483_residues
MSAPIPNVRPAPDSVLVEIADYALNHRIDSALALETARHCLIDTLGCGLEALSYPACTKLLGPIVPGTIVPHGAKVPGTS
LQLDPVQAAFNIGAAIRWLDFNDTWLAAEWGHPSDNLGGILATADWLSRTAVASGKRPLAMKDVLVAMIHAHEIQGCLAL
ENSFNAVGLDHVLLVKVASTATVGCLLGLTRDELINALSLAFVDGQALRTYRHAPNTGSRKSWAAGDATSRAVRLALIAK
TGEMGYPRALTAKTWGFYDVSFGGKPFRFQRPYGTYVMENVLFKLSYPAEFHAQTAVEAAMTLHARLAAAGRTADEIRRV
TVRTHAAAMRIIDKQGPLANPADRDHCIQYMIAVPLLFGRLTAADYEDAVAADPRIDALRAKIACVEDPQFTQDYHDPAK
RSIANALTIEFDDGSTLAEVAVEYPIGHMRRRAEGMALLIDKFRTNLARRFPAKQQQAILDVSLDQAKLEAMPVDEYVDL
YVI

Sequences:

>Translated_483_residues
MSAPIPNVRPAPDSVLVEIADYALNHRIDSALALETARHCLIDTLGCGLEALSYPACTKLLGPIVPGTIVPHGAKVPGTS
LQLDPVQAAFNIGAAIRWLDFNDTWLAAEWGHPSDNLGGILATADWLSRTAVASGKRPLAMKDVLVAMIHAHEIQGCLAL
ENSFNAVGLDHVLLVKVASTATVGCLLGLTRDELINALSLAFVDGQALRTYRHAPNTGSRKSWAAGDATSRAVRLALIAK
TGEMGYPRALTAKTWGFYDVSFGGKPFRFQRPYGTYVMENVLFKLSYPAEFHAQTAVEAAMTLHARLAAAGRTADEIRRV
TVRTHAAAMRIIDKQGPLANPADRDHCIQYMIAVPLLFGRLTAADYEDAVAADPRIDALRAKIACVEDPQFTQDYHDPAK
RSIANALTIEFDDGSTLAEVAVEYPIGHMRRRAEGMALLIDKFRTNLARRFPAKQQQAILDVSLDQAKLEAMPVDEYVDL
YVI
>Mature_482_residues
SAPIPNVRPAPDSVLVEIADYALNHRIDSALALETARHCLIDTLGCGLEALSYPACTKLLGPIVPGTIVPHGAKVPGTSL
QLDPVQAAFNIGAAIRWLDFNDTWLAAEWGHPSDNLGGILATADWLSRTAVASGKRPLAMKDVLVAMIHAHEIQGCLALE
NSFNAVGLDHVLLVKVASTATVGCLLGLTRDELINALSLAFVDGQALRTYRHAPNTGSRKSWAAGDATSRAVRLALIAKT
GEMGYPRALTAKTWGFYDVSFGGKPFRFQRPYGTYVMENVLFKLSYPAEFHAQTAVEAAMTLHARLAAAGRTADEIRRVT
VRTHAAAMRIIDKQGPLANPADRDHCIQYMIAVPLLFGRLTAADYEDAVAADPRIDALRAKIACVEDPQFTQDYHDPAKR
SIANALTIEFDDGSTLAEVAVEYPIGHMRRRAEGMALLIDKFRTNLARRFPAKQQQAILDVSLDQAKLEAMPVDEYVDLY
VI

Specific function: Catalyzes the dehydration of 2-methylcitrate to 2- methyl-cis-aconitate [H]

COG id: COG2079

COG function: function code R; Uncharacterized protein involved in propionate catabolism

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prpD family [H]

Homologues:

Organism=Escherichia coli, GI1786528, Length=483, Percent_Identity=75.9834368530021, Blast_Score=789, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6325258, Length=483, Percent_Identity=60.4554865424431, Blast_Score=588, Evalue=1e-169,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012705
- InterPro:   IPR005656 [H]

Pfam domain/function: PF03972 MmgE_PrpD [H]

EC number: =4.2.1.79 [H]

Molecular weight: Translated: 52471; Mature: 52340

Theoretical pI: Translated: 6.75; Mature: 6.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAPIPNVRPAPDSVLVEIADYALNHRIDSALALETARHCLIDTLGCGLEALSYPACTKL
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCHHHHHH
LGPIVPGTIVPHGAKVPGTSLQLDPVQAAFNIGAAIRWLDFNDTWLAAEWGHPSDNLGGI
HHCCCCCCCCCCCCCCCCCCEEECHHHHHHCCCCEEEEEECCCCEEEEECCCCCCCCCCE
LATADWLSRTAVASGKRPLAMKDVLVAMIHAHEIQGCLALENSFNAVGLDHVLLVKVAST
EEHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCCEEEEEEHHH
ATVGCLLGLTRDELINALSLAFVDGQALRTYRHAPNTGSRKSWAAGDATSRAVRLALIAK
HHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCCCCCCHHHEEEEEEEEE
TGEMGYPRALTAKTWGFYDVSFGGKPFRFQRPYGTYVMENVLFKLSYPAEFHAQTAVEAA
CCCCCCCCEEEECCCCEEEECCCCCCEEEECCCHHHHHHHHHHEECCCHHHHHHHHHHHH
MTLHARLAAAGRTADEIRRVTVRTHAAAMRIIDKQGPLANPADRDHCIQYMIAVPLLFGR
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHH
LTAADYEDAVAADPRIDALRAKIACVEDPQFTQDYHDPAKRSIANALTIEFDDGSTLAEV
HHCCCCCHHHCCCCCHHHHHHEEEEECCCCCCHHHCCHHHHHHHCEEEEEECCCCHHHHH
AVEYPIGHMRRRAEGMALLIDKFRTNLARRFPAKQQQAILDVSLDQAKLEAMPVDEYVDL
HHHCCHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHEEEEEECCHHHHHCCCCCCCEEE
YVI
EEC
>Mature Secondary Structure 
SAPIPNVRPAPDSVLVEIADYALNHRIDSALALETARHCLIDTLGCGLEALSYPACTKL
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCHHHHHH
LGPIVPGTIVPHGAKVPGTSLQLDPVQAAFNIGAAIRWLDFNDTWLAAEWGHPSDNLGGI
HHCCCCCCCCCCCCCCCCCCEEECHHHHHHCCCCEEEEEECCCCEEEEECCCCCCCCCCE
LATADWLSRTAVASGKRPLAMKDVLVAMIHAHEIQGCLALENSFNAVGLDHVLLVKVAST
EEHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCCEEEEEEHHH
ATVGCLLGLTRDELINALSLAFVDGQALRTYRHAPNTGSRKSWAAGDATSRAVRLALIAK
HHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCCCCCCHHHEEEEEEEEE
TGEMGYPRALTAKTWGFYDVSFGGKPFRFQRPYGTYVMENVLFKLSYPAEFHAQTAVEAA
CCCCCCCCEEEECCCCEEEECCCCCCEEEECCCHHHHHHHHHHEECCCHHHHHHHHHHHH
MTLHARLAAAGRTADEIRRVTVRTHAAAMRIIDKQGPLANPADRDHCIQYMIAVPLLFGR
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHH
LTAADYEDAVAADPRIDALRAKIACVEDPQFTQDYHDPAKRSIANALTIEFDDGSTLAEV
HHCCCCCHHHCCCCCHHHHHHEEEEECCCCCCHHHCCHHHHHHHCEEEEEECCCCHHHHH
AVEYPIGHMRRRAEGMALLIDKFRTNLARRFPAKQQQAILDVSLDQAKLEAMPVDEYVDL
HHHCCHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHEEEEEECCHHHHHCCCCCCCEEE
YVI
EEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]