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The map label for this gene is proB [H]

Identifier: 226830768

GI number: 226830768

Start: 3427825

End: 3429009

Strand: Reverse

Name: proB [H]

Synonym: BURPS1106A_3523

Alternate gene names: 226830768

Gene position: 3429009-3427825 (Counterclockwise)

Preceding gene: 126451715

Following gene: 126455301

Centisome position: 85.97

GC content: 68.27

Gene sequence:

>1185_bases
ATGAAGCCGGGCGGCGCGGCCGGCGTACGGGATTCAATCGCGCATCACTCAAGGAGACAGCGCACGATGCGTTCGATCAT
CGCCGATTCGAAGCGCCTGGTAGTGAAAGTGGGCTCGAGCCTCGTCACCAACGACGGCCGCGGGCTCGATCATGACGCCA
TCGGCCGATGGGCCGCCCAGATCGCCGCGCTGCGCGGCGCGGGCAAGGAAGTGGTGCTCGTCAGCTCGGGCGCGATCGCG
GAAGGCATGCAGCGCCTCGGTTGGAGCAAGCGGCCGCGCGAGATCGACGAGTTGCAAGCGGCCGCGGCCGTCGGGCAAAT
GGGGCTCGCGCAGGTATACGAGAGCCGGTTTGCCGAACACGGGATCCGCACCGCGCAGATTCTCCTCACGCATGCGGATC
TCGCCGACCGCGAACGCTACCTGAACGCGCGCTCGACGCTCCTCACGCTGCTGCGGCTCGGCGTCGTGCCGATCATCAAC
GAGAACGACACCGTCGTCACCGACGAAATCAAGTTCGGCGACAACGACACGCTCGGCGCGCTCGTCGCGAATCTGATCGA
AGGCGATACGCTCGTCATCCTGACCGACCAGCCGGGGCTGTTCACGGCCGATCCGCGCAAGGATCCGGGCGCGACGCTCG
TTGCCGAGGCGAGCGCCGGTGCGCCGGAGCTCGAAGCGATGGCGGGCGGCGCGGGCTCGAGCATCGGCCGCGGCGGCATG
CTGACGAAGATTCTCGCGGCCAAGCGCGCCGCGCACAGCGGCGCGAACACCGTGATCGCGAGCGGCCGCGAGCGCGACGT
GCTCGTGCGGCTCGCGGCCGGCGAGGCGATCGGCACGCAACTGATCGCGCGCACCGCGCGGATGGCGGCGCGCAAGCAAT
GGATGGCGGACCACCTGCAGGTACGTGGCCATGTCGTTATCGATGCGGGGGCGGTCGACAAGTTGACGGCGGGGGGCAAG
AGCCTGTTGCCGATCGGCGTCGTCGCCGTGCAGGGCGTGTTCGCGCGCGGCGAAGTGATCGCGTGCGTCGACGACACCGG
CCGTGAAGTCGCGCGCGGGATCACCAATTACAGCAGCGCCGAAACGAAGCTGATCCAGCGCAAGCCGAGCGGCGAGATCG
AAACGGTGCTCGGCTACATGCTCGAGCCCGAGCTGATCCATCGGGACAATCTCGTGCTGGTCTGA

Upstream 100 bases:

>100_bases
GGATTTGGCGTCGGACGTGCGCTTTCGCGATGCGCCCGGCGCAGGCGGCGAGCCGCACGAGCGCGACGCCGGCGCGCACT
GAGCGTCTCGTCCATCGCCC

Downstream 100 bases:

>100_bases
CGGCGACATGCGCGCCGCGCCTCGGTCGCGGCGGCGGAAGGCGACAAAAAAGCCCGCGCATGCGGGCTTTTTGCTTTCGT
CAGCCTGTCGAACCTCGGTT

Product: gamma-glutamyl kinase

Products: NA

Alternate protein names: Gamma-glutamyl kinase; GK [H]

Number of amino acids: Translated: 394; Mature: 394

Protein sequence:

>394_residues
MKPGGAAGVRDSIAHHSRRQRTMRSIIADSKRLVVKVGSSLVTNDGRGLDHDAIGRWAAQIAALRGAGKEVVLVSSGAIA
EGMQRLGWSKRPREIDELQAAAAVGQMGLAQVYESRFAEHGIRTAQILLTHADLADRERYLNARSTLLTLLRLGVVPIIN
ENDTVVTDEIKFGDNDTLGALVANLIEGDTLVILTDQPGLFTADPRKDPGATLVAEASAGAPELEAMAGGAGSSIGRGGM
LTKILAAKRAAHSGANTVIASGRERDVLVRLAAGEAIGTQLIARTARMAARKQWMADHLQVRGHVVIDAGAVDKLTAGGK
SLLPIGVVAVQGVFARGEVIACVDDTGREVARGITNYSSAETKLIQRKPSGEIETVLGYMLEPELIHRDNLVLV

Sequences:

>Translated_394_residues
MKPGGAAGVRDSIAHHSRRQRTMRSIIADSKRLVVKVGSSLVTNDGRGLDHDAIGRWAAQIAALRGAGKEVVLVSSGAIA
EGMQRLGWSKRPREIDELQAAAAVGQMGLAQVYESRFAEHGIRTAQILLTHADLADRERYLNARSTLLTLLRLGVVPIIN
ENDTVVTDEIKFGDNDTLGALVANLIEGDTLVILTDQPGLFTADPRKDPGATLVAEASAGAPELEAMAGGAGSSIGRGGM
LTKILAAKRAAHSGANTVIASGRERDVLVRLAAGEAIGTQLIARTARMAARKQWMADHLQVRGHVVIDAGAVDKLTAGGK
SLLPIGVVAVQGVFARGEVIACVDDTGREVARGITNYSSAETKLIQRKPSGEIETVLGYMLEPELIHRDNLVLV
>Mature_394_residues
MKPGGAAGVRDSIAHHSRRQRTMRSIIADSKRLVVKVGSSLVTNDGRGLDHDAIGRWAAQIAALRGAGKEVVLVSSGAIA
EGMQRLGWSKRPREIDELQAAAAVGQMGLAQVYESRFAEHGIRTAQILLTHADLADRERYLNARSTLLTLLRLGVVPIIN
ENDTVVTDEIKFGDNDTLGALVANLIEGDTLVILTDQPGLFTADPRKDPGATLVAEASAGAPELEAMAGGAGSSIGRGGM
LTKILAAKRAAHSGANTVIASGRERDVLVRLAAGEAIGTQLIARTARMAARKQWMADHLQVRGHVVIDAGAVDKLTAGGK
SLLPIGVVAVQGVFARGEVIACVDDTGREVARGITNYSSAETKLIQRKPSGEIETVLGYMLEPELIHRDNLVLV

Specific function: Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5- oxoproline [H]

COG id: COG0263

COG function: function code E; Glutamate 5-kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain [H]

Homologues:

Organism=Homo sapiens, GI21361368, Length=294, Percent_Identity=35.7142857142857, Blast_Score=137, Evalue=1e-32,
Organism=Homo sapiens, GI62912457, Length=292, Percent_Identity=35.958904109589, Blast_Score=137, Evalue=1e-32,
Organism=Escherichia coli, GI1786437, Length=366, Percent_Identity=43.1693989071038, Blast_Score=276, Evalue=1e-75,
Organism=Caenorhabditis elegans, GI17569967, Length=278, Percent_Identity=34.8920863309352, Blast_Score=142, Evalue=3e-34,
Organism=Caenorhabditis elegans, GI17569969, Length=278, Percent_Identity=34.8920863309352, Blast_Score=142, Evalue=3e-34,
Organism=Saccharomyces cerevisiae, GI6320506, Length=423, Percent_Identity=34.9881796690307, Blast_Score=207, Evalue=2e-54,
Organism=Saccharomyces cerevisiae, GI6321822, Length=417, Percent_Identity=32.8537170263789, Blast_Score=194, Evalue=3e-50,
Organism=Drosophila melanogaster, GI21357643, Length=354, Percent_Identity=32.2033898305085, Blast_Score=147, Evalue=1e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001048
- InterPro:   IPR001057
- InterPro:   IPR011529
- InterPro:   IPR005715
- InterPro:   IPR019797
- InterPro:   IPR002478
- InterPro:   IPR015947 [H]

Pfam domain/function: PF00696 AA_kinase; PF01472 PUA [H]

EC number: =2.7.2.11 [H]

Molecular weight: Translated: 41604; Mature: 41604

Theoretical pI: Translated: 9.01; Mature: 9.01

Prosite motif: PS50890 PUA ; PS00902 GLUTAMATE_5_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPGGAAGVRDSIAHHSRRQRTMRSIIADSKRLVVKVGSSLVTNDGRGLDHDAIGRWAAQ
CCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCHHCCCCCCCCCHHHHHHHHHH
IAALRGAGKEVVLVSSGAIAEGMQRLGWSKRPREIDELQAAAAVGQMGLAQVYESRFAEH
HHHHHCCCCEEEEEECCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHC
GIRTAQILLTHADLADRERYLNARSTLLTLLRLGVVPIINENDTVVTDEIKFGDNDTLGA
CCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCEEEECCCCEEEEEEEECCCCCHHHH
LVANLIEGDTLVILTDQPGLFTADPRKDPGATLVAEASAGAPELEAMAGGAGSSIGRGGM
HHHHHHCCCEEEEEECCCCEEECCCCCCCCCEEEEECCCCCCCHHHHCCCCCCCCCCCHH
LTKILAAKRAAHSGANTVIASGRERDVLVRLAAGEAIGTQLIARTARMAARKQWMADHLQ
HHHHHHHHHHHCCCCCEEEECCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHH
VRGHVVIDAGAVDKLTAGGKSLLPIGVVAVQGVFARGEVIACVDDTGREVARGITNYSSA
CCCEEEEECCCCHHHCCCCCCCCHHHHHHHHHHEECCCEEEEECCCHHHHHHHHCCCCHH
ETKLIQRKPSGEIETVLGYMLEPELIHRDNLVLV
HHHHHHCCCCCCHHHHHHHHCCCHHCCCCCEEEC
>Mature Secondary Structure
MKPGGAAGVRDSIAHHSRRQRTMRSIIADSKRLVVKVGSSLVTNDGRGLDHDAIGRWAAQ
CCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCHHCCCCCCCCCHHHHHHHHHH
IAALRGAGKEVVLVSSGAIAEGMQRLGWSKRPREIDELQAAAAVGQMGLAQVYESRFAEH
HHHHHCCCCEEEEEECCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHC
GIRTAQILLTHADLADRERYLNARSTLLTLLRLGVVPIINENDTVVTDEIKFGDNDTLGA
CCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCEEEECCCCEEEEEEEECCCCCHHHH
LVANLIEGDTLVILTDQPGLFTADPRKDPGATLVAEASAGAPELEAMAGGAGSSIGRGGM
HHHHHHCCCEEEEEECCCCEEECCCCCCCCCEEEEECCCCCCCHHHHCCCCCCCCCCCHH
LTKILAAKRAAHSGANTVIASGRERDVLVRLAAGEAIGTQLIARTARMAARKQWMADHLQ
HHHHHHHHHHHCCCCCEEEECCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHH
VRGHVVIDAGAVDKLTAGGKSLLPIGVVAVQGVFARGEVIACVDDTGREVARGITNYSSA
CCCEEEEECCCCHHHCCCCCCCCHHHHHHHHHHEECCCEEEEECCCHHHHHHHHCCCCHH
ETKLIQRKPSGEIETVLGYMLEPELIHRDNLVLV
HHHHHHCCCCCCHHHHHHHHCCCHHCCCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA