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The map label for this gene is narX [H]

Identifier: 126455395

GI number: 126455395

Start: 2650245

End: 2652179

Strand: Direct

Name: narX [H]

Synonym: BURPS1106A_2685

Alternate gene names: 126455395

Gene position: 2650245-2652179 (Clockwise)

Preceding gene: 126452433

Following gene: 126452622

Centisome position: 66.45

GC content: 71.42

Gene sequence:

>1935_bases
ATGGCTCCCGCCCTCCCCGACTCCCCCGCTCCGTGGCGGCACCGCCTGTCGACGCGGATCGTCGCGCTGTCCGCGATCGC
GTGGGCGCTCGTGCTCGCGATGGTGGGCGGCACGCTGTGGCTGTCCTGGCAGCTCGAGGGCGCGGGCGCGGCGATCAACG
ACGCCGGCAGCCTGCGCATGCGCGCGACGCGCACCTACGTCGAGCTGTCGGGCGTGGGCACCGAGCCGCGCCGCCAGCTC
GCGGTCGAGCTCGACGCGATCGACGGCACGCTCGCGCGGCTGCGCCGCGGCGATCCGTCGCGCCCCCTCTTTCTGCCGAA
CACCACCGTCATCCAGCATCAGCTCGACATCGTCACCGATCGCTGGAACGCCGTGCTGCGCCCGCTCGCCGCCGGCGCGC
TGACGAACGCGCCGCGCGCTGACGCGGCCGCCGCCTACCTCGTCGCGCTGCCCGGCTTCGTCGACGAGGCCGATCTGCTC
GTGCGCGAGATCGAATCCGACAACGCGCGCAAGACGACGTGGCTGCGCCTGTCGCAGATCGGGCTCGGCCTGCTCGCGTG
CTCGGGCACGGTCGCCGTCATCTACCTGCTGTTCCTGTGGATCATCCTGCCCGTGATGCAGTTGCGCGACGGCCTGAAGC
GCATGGCCGCGCGCGAGTTCGCGGTGCGCCTGCCCGTGCAGACGCGCGACGAGTTCGGCGATCTCGCGCACGGCTTCAAC
CGGATGGCAAGCGAATTGCAGGAGGTGTACGCGGGGCTCGAGGAGCGCGTGCAGCAAAAGACCGCGCAGCTCGCCGCGCA
GAACCGCGAGCTGAGCGCGCTCTACGAGATCACCGCGTTCCTGAACCGCCCGCAGGCCGTCGACGAGATGTGCGCGGGCT
TCCTGTCGCGCGCGATCGCGCAGTTCGACGCCGACGCGGGCAGCATCCGCGTCACCGATCCGACGGGCGAAAAGCTGCAT
CTCGTGATTGCGGAGGGCCTGTCGACCGAGCTCACCGAGCTCGAACGCTGCATGCCCGTCGACGATTGCTTCTGCGGCAC
CGTGACGCGCGCGGACACGGCCGTGCTGCACGACCTGCGCGGCAGCGCCGCGCGCGCGCCGACGCCGTGCTCGCGCGAGG
GCTTCGCGGCCGTCGCGGTGTTCAAGGTGATGACCCAGGACGCGGTGCTCGGCTCGTTCTCGCTGCACTATCGCGACCCG
TCGCGGCTGCCCGACGCCGAGCGGCGCGTGCTCGAGACGCTCGGCCGCCATCTCGGAATCGCGCTCGATCACGTGCGGCT
GTCCGCGAGCGCGCGGCAGCTCGCGGTCGCCGAAGAGCGCAATCTCGTCGCGCAGGGCCTGCACGACAGCATCGCGCAAA
GCCTCAATTTCGTGAACCTGCAGACGCAGATGCTCGGCGACGCGATCGCGCACGACAATCTCGCCGATGCGCGCGAGATC
GTGCCGATGCTCAAGCACGGCGTCGAGCAGGGCTACGCGGACGTGCGCGAGCTGCTGCTCAACTTCCGCACGCGCTTGAC
GCAGGGCGAGCTGAAAGCGGCGATCGAGGAAACGATCGCGCGCTTCGAGAAGCAGACGCGCATCGCGTGCGCGCTGAACT
ATCGCGAGACGGGCGGCGCGCCGCTGCCGCCCGACGAGCAGTTGCAGGTGCTGTTCATCCTGCAGGAAGCGCTGTCGAAC
GTGCGCAAGCATGCGCACGCGCGGCATGTCGCGGTCGATGTCGAGAACGGCGCCGCGTTCCGGCTGTCCGTCGCCGACGA
CGGCTGCGGCTACGATCCGGCCGCGCTCGCCGCGCGCGCGGACACGCATGTCGGGCTCACGATCATGCGCGAGCGCGCCG
CCCGCCTGCGCGCGACGCTCACGTTGACGGGCGCGCCGCAGCGCGGCGCGCGCGTCGAGCTGGTGCTGCCGGCCGACGCG
CGACAGGCGGCATAG

Upstream 100 bases:

>100_bases
CCGGGCGGGTGCGCCGCGATGCCTCACGTGCTCGAATGTCGCAGCAGGGGCGCAACATTCGGCAGGACAATTGTCATGTC
CAAAAAAATCATGACGTGCT

Downstream 100 bases:

>100_bases
CAACGAGAACGAGGACAATAATCATGACCATACGGGTACTGTTGATCGACGACCACACGCTCTTTCGCAGCGGCGTCAAG
CTGCTGCTGCAGCGCCAGCC

Product: nitrate/nitrite sensor protein NarX

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 644; Mature: 643

Protein sequence:

>644_residues
MAPALPDSPAPWRHRLSTRIVALSAIAWALVLAMVGGTLWLSWQLEGAGAAINDAGSLRMRATRTYVELSGVGTEPRRQL
AVELDAIDGTLARLRRGDPSRPLFLPNTTVIQHQLDIVTDRWNAVLRPLAAGALTNAPRADAAAAYLVALPGFVDEADLL
VREIESDNARKTTWLRLSQIGLGLLACSGTVAVIYLLFLWIILPVMQLRDGLKRMAAREFAVRLPVQTRDEFGDLAHGFN
RMASELQEVYAGLEERVQQKTAQLAAQNRELSALYEITAFLNRPQAVDEMCAGFLSRAIAQFDADAGSIRVTDPTGEKLH
LVIAEGLSTELTELERCMPVDDCFCGTVTRADTAVLHDLRGSAARAPTPCSREGFAAVAVFKVMTQDAVLGSFSLHYRDP
SRLPDAERRVLETLGRHLGIALDHVRLSASARQLAVAEERNLVAQGLHDSIAQSLNFVNLQTQMLGDAIAHDNLADAREI
VPMLKHGVEQGYADVRELLLNFRTRLTQGELKAAIEETIARFEKQTRIACALNYRETGGAPLPPDEQLQVLFILQEALSN
VRKHAHARHVAVDVENGAAFRLSVADDGCGYDPAALAARADTHVGLTIMRERAARLRATLTLTGAPQRGARVELVLPADA
RQAA

Sequences:

>Translated_644_residues
MAPALPDSPAPWRHRLSTRIVALSAIAWALVLAMVGGTLWLSWQLEGAGAAINDAGSLRMRATRTYVELSGVGTEPRRQL
AVELDAIDGTLARLRRGDPSRPLFLPNTTVIQHQLDIVTDRWNAVLRPLAAGALTNAPRADAAAAYLVALPGFVDEADLL
VREIESDNARKTTWLRLSQIGLGLLACSGTVAVIYLLFLWIILPVMQLRDGLKRMAAREFAVRLPVQTRDEFGDLAHGFN
RMASELQEVYAGLEERVQQKTAQLAAQNRELSALYEITAFLNRPQAVDEMCAGFLSRAIAQFDADAGSIRVTDPTGEKLH
LVIAEGLSTELTELERCMPVDDCFCGTVTRADTAVLHDLRGSAARAPTPCSREGFAAVAVFKVMTQDAVLGSFSLHYRDP
SRLPDAERRVLETLGRHLGIALDHVRLSASARQLAVAEERNLVAQGLHDSIAQSLNFVNLQTQMLGDAIAHDNLADAREI
VPMLKHGVEQGYADVRELLLNFRTRLTQGELKAAIEETIARFEKQTRIACALNYRETGGAPLPPDEQLQVLFILQEALSN
VRKHAHARHVAVDVENGAAFRLSVADDGCGYDPAALAARADTHVGLTIMRERAARLRATLTLTGAPQRGARVELVLPADA
RQAA
>Mature_643_residues
APALPDSPAPWRHRLSTRIVALSAIAWALVLAMVGGTLWLSWQLEGAGAAINDAGSLRMRATRTYVELSGVGTEPRRQLA
VELDAIDGTLARLRRGDPSRPLFLPNTTVIQHQLDIVTDRWNAVLRPLAAGALTNAPRADAAAAYLVALPGFVDEADLLV
REIESDNARKTTWLRLSQIGLGLLACSGTVAVIYLLFLWIILPVMQLRDGLKRMAAREFAVRLPVQTRDEFGDLAHGFNR
MASELQEVYAGLEERVQQKTAQLAAQNRELSALYEITAFLNRPQAVDEMCAGFLSRAIAQFDADAGSIRVTDPTGEKLHL
VIAEGLSTELTELERCMPVDDCFCGTVTRADTAVLHDLRGSAARAPTPCSREGFAAVAVFKVMTQDAVLGSFSLHYRDPS
RLPDAERRVLETLGRHLGIALDHVRLSASARQLAVAEERNLVAQGLHDSIAQSLNFVNLQTQMLGDAIAHDNLADAREIV
PMLKHGVEQGYADVRELLLNFRTRLTQGELKAAIEETIARFEKQTRIACALNYRETGGAPLPPDEQLQVLFILQEALSNV
RKHAHARHVAVDVENGAAFRLSVADDGCGYDPAALAARADTHVGLTIMRERAARLRATLTLTGAPQRGARVELVLPADAR
QAA

Specific function: Acts as a sensor for nitrate/nitrite and transduces signal of nitrate availability to the narL protein and of both nitrate/nitrite to the narP protein. NarX probably activates narL and narP by phosphorylation in the presence of nitrate. NarX also plays a

COG id: COG3850

COG function: function code T; Signal transduction histidine kinase, nitrate/nitrite-specific

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1787474, Length=623, Percent_Identity=28.4109149277689, Blast_Score=148, Evalue=1e-36,
Organism=Escherichia coli, GI1788812, Length=502, Percent_Identity=27.6892430278884, Blast_Score=132, Evalue=5e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR016380
- InterPro:   IPR003661
- InterPro:   IPR011712
- InterPro:   IPR005467 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF07730 HisKA_3 [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 70183; Mature: 70052

Theoretical pI: Translated: 6.46; Mature: 6.46

Prosite motif: PS50885 HAMP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAPALPDSPAPWRHRLSTRIVALSAIAWALVLAMVGGTLWLSWQLEGAGAAINDAGSLRM
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEECCCCCEECCCCCEEE
RATRTYVELSGVGTEPRRQLAVELDAIDGTLARLRRGDPSRPLFLPNTTVIQHQLDIVTD
EEEEEEEEEECCCCCHHHHHEEEEECCCHHHHHHHCCCCCCCEECCCCHHHHHHHHHHHH
RWNAVLRPLAAGALTNAPRADAAAAYLVALPGFVDEADLLVREIESDNARKTTWLRLSQI
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCHHHHEEHHHH
GLGLLACSGTVAVIYLLFLWIILPVMQLRDGLKRMAAREFAVRLPVQTRDEFGDLAHGFN
CCHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
RMASELQEVYAGLEERVQQKTAQLAAQNRELSALYEITAFLNRPQAVDEMCAGFLSRAIA
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
QFDADAGSIRVTDPTGEKLHLVIAEGLSTELTELERCMPVDDCFCGTVTRADTAVLHDLR
HHCCCCCCEEEECCCCCEEEEEEECCCCHHHHHHHHCCCCCHHHCCCCCHHHHHHHHHHC
GSAARAPTPCSREGFAAVAVFKVMTQDAVLGSFSLHYRDPSRLPDAERRVLETLGRHLGI
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCEEEEECCCCCCCHHHHHHHHHHHHHHCE
ALDHVRLSASARQLAVAEERNLVAQGLHDSIAQSLNFVNLQTQMLGDAIAHDNLADAREI
EHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
VPMLKHGVEQGYADVRELLLNFRTRLTQGELKAAIEETIARFEKQTRIACALNYRETGGA
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCEEEEEEECCCCCCC
PLPPDEQLQVLFILQEALSNVRKHAHARHVAVDVENGAAFRLSVADDGCGYDPAALAARA
CCCCCCHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCEEEEEECCCCCCCCHHHHHHCC
DTHVGLTIMRERAARLRATLTLTGAPQRGARVELVLPADARQAA
CCCCHHHHHHHHHHHHEEEEEEECCCCCCCEEEEEECCCCCCCC
>Mature Secondary Structure 
APALPDSPAPWRHRLSTRIVALSAIAWALVLAMVGGTLWLSWQLEGAGAAINDAGSLRM
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEECCCCCEECCCCCEEE
RATRTYVELSGVGTEPRRQLAVELDAIDGTLARLRRGDPSRPLFLPNTTVIQHQLDIVTD
EEEEEEEEEECCCCCHHHHHEEEEECCCHHHHHHHCCCCCCCEECCCCHHHHHHHHHHHH
RWNAVLRPLAAGALTNAPRADAAAAYLVALPGFVDEADLLVREIESDNARKTTWLRLSQI
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCHHHHEEHHHH
GLGLLACSGTVAVIYLLFLWIILPVMQLRDGLKRMAAREFAVRLPVQTRDEFGDLAHGFN
CCHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
RMASELQEVYAGLEERVQQKTAQLAAQNRELSALYEITAFLNRPQAVDEMCAGFLSRAIA
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
QFDADAGSIRVTDPTGEKLHLVIAEGLSTELTELERCMPVDDCFCGTVTRADTAVLHDLR
HHCCCCCCEEEECCCCCEEEEEEECCCCHHHHHHHHCCCCCHHHCCCCCHHHHHHHHHHC
GSAARAPTPCSREGFAAVAVFKVMTQDAVLGSFSLHYRDPSRLPDAERRVLETLGRHLGI
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCEEEEECCCCCCCHHHHHHHHHHHHHHCE
ALDHVRLSASARQLAVAEERNLVAQGLHDSIAQSLNFVNLQTQMLGDAIAHDNLADAREI
EHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
VPMLKHGVEQGYADVRELLLNFRTRLTQGELKAAIEETIARFEKQTRIACALNYRETGGA
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCEEEEEEECCCCCCC
PLPPDEQLQVLFILQEALSNVRKHAHARHVAVDVENGAAFRLSVADDGCGYDPAALAARA
CCCCCCHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCEEEEEECCCCCCCCHHHHHHCC
DTHVGLTIMRERAARLRATLTLTGAPQRGARVELVLPADARQAA
CCCCHHHHHHHHHHHHEEEEEEECCCCCCCEEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]