Click here to switch to the map view.

The map label for this gene is proS [H]

Identifier: 126455301

GI number: 126455301

Start: 3424368

End: 3426104

Strand: Reverse

Name: proS [H]

Synonym: BURPS1106A_3520

Alternate gene names: 126455301

Gene position: 3426104-3424368 (Counterclockwise)

Preceding gene: 226830768

Following gene: 126453993

Centisome position: 85.9

GC content: 66.49

Gene sequence:

>1737_bases
ATGAAAGCCTCCCGTTTCTTTATCGGCACCTTGAAGGAAGCACCCGCCGACGCCGAGATCGTCAGCCACAAGCTGATGGT
GCGAGCCGGCATGATTCGCCGCGTCGCCGGCGGCATCTATAACTATCTGCCGGTCGGCCTGCGCTCGATCCGCAAGGTCG
AGGCGATCGTGCGCGAGGAGATGAACCGGGCGGGCGCGATCGAGCTGCTGATGCCCGCCGTGCAGCCCGCCGAGCTGTGG
CAGGAATCGGGGCGCTGGGAGCAGTACGGTCCCGAACTGCTGCGCTTCAAGGATCGCAAGCAGAACGAATTCGTGATCGG
GCCGACGCACGAGGAAGTCGTCACTGACATCGCGCGCAACCAGATCAAGAGCTACCGGCAGATGCCGGTGAACTTCTACC
AGATCCAGACGAAGTTCCGCGACGAGATCCGTCCGCGCTTCGGCGTGATGCGCGGCCGCGAGTTCATCATGAAGGACGCG
TACTCGTTTGATAAGGATCACGAAAGCCTCAAGGAATCGTACAAGAAGATGTACGACGCGTACGTGCGGATCTTCACGCG
CATCGGCCTCGAGTTTCGTCCGGTCGCGGCCGACAACGGCTCGATCGGCGGCAGCGGCTCGCATGAGTTTCATGTGATCG
CGGATACCGGCGAGGACGCGATCGCCTATTGCCCGACGTCCGACTTCGCGGCGAACGTCGAGGCCGCCGAGGCGCTGCCG
CTGCTCGCGAGCCGCGCGGCGCCCGCCGAGGCGATGCAAAAGGTCGCGACGCCCGGCAAGGCGAAGTGCGAGGCGGTGGC
CGAGCTGATGGGCATCCCGCTCGAGCGCACGATCAAGTCGATCGTGCTCGCGACCGACAACGAAGGCGCCGAGCCGACGA
TCTGGCTGCTGATGCTGCGCGGCGATCACGATCTGAACGAGATCAAGACGGCGAAGCTGCCGGGCCTGGCCGGCCACCGC
TTCGCGACCGAGGCGGAGATCGTCGAGTGGTTCGGCACGCCGCCCGGCTATCTCGGCCCGATCGGCACGAAGAAGCCGGT
GCGCGTGGTCGCCGATCGCACGGTCGCGAACATGAGCGATTTCGTCGTCGGCGCGAACGAGGTCGATTACCACATCGCCG
GCGTGAACTGGGGGCGCGACCTGCCGGAGCCCGTCGTCGCCGACATCCGCAACGTGAAGGCGGGCGATCCGTCGCCGGAC
GGCAAGGGCGCGCTCGACATCTGCCGCGGCATTGAAGTCGGCCATGTGTTCCAGCTCGGCACCAAGTATTCGGACGCGAT
GGGCGCGACGTTCATCGACGAATCGGGCAAGGCGCAGCCGATGGTGATGGGTTGCTACGGGATCGGCATCACGCGGATTC
TCGGCGCGGCGATCGAACAGAACTTCGACGACAAGGGTATCGTCTGGCCCGAGGCGATCGCGCCGTTCGAGGTCGTGCTG
TGCCCGATGGGCTACGACCGCAGCGATGCGGTGCGCGAAGCGGCCGACAAGCTCTACGCGGACCTCGCCGCCGCGGGCAT
CGACGTGATCCTCGACGATCGCGGCGAGCGTCCGGGCGTGATGTTCGCGGATTGGGAACTGATCGGCGTGCCGCATCGCC
TCGTGATCGGCGAGCGCGGCCTGAAGGACGGCAAGATCGAGTACCAGGGCCGCCGCGACGCCGAGGCGACGCTGCTGCCC
GCCGATTCGGCCGCGGCGGCGGTCGCCGAAAAGGTCCGCGCGGCGCTCGCCCGCTGA

Upstream 100 bases:

>100_bases
GGTATGTCATCTGCGCCGCATACTGCCGGGCGCAGCCGTTAATTCACCTTTAGACTTTTTCGGGCGGCCCGCGTTGGCCG
CTGCTCATGGAACCGTTCGC

Downstream 100 bases:

>100_bases
TCGCCGGTCATCGACGAAGCGGAGGGGCGCGTTGGAGTCGAATTTCCTGTCGGCGACGGTGCTCCTCATTCTCATTACCG
ATCCGCTCGGCAACATCCCG

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase; ProRS [H]

Number of amino acids: Translated: 578; Mature: 578

Protein sequence:

>578_residues
MKASRFFIGTLKEAPADAEIVSHKLMVRAGMIRRVAGGIYNYLPVGLRSIRKVEAIVREEMNRAGAIELLMPAVQPAELW
QESGRWEQYGPELLRFKDRKQNEFVIGPTHEEVVTDIARNQIKSYRQMPVNFYQIQTKFRDEIRPRFGVMRGREFIMKDA
YSFDKDHESLKESYKKMYDAYVRIFTRIGLEFRPVAADNGSIGGSGSHEFHVIADTGEDAIAYCPTSDFAANVEAAEALP
LLASRAAPAEAMQKVATPGKAKCEAVAELMGIPLERTIKSIVLATDNEGAEPTIWLLMLRGDHDLNEIKTAKLPGLAGHR
FATEAEIVEWFGTPPGYLGPIGTKKPVRVVADRTVANMSDFVVGANEVDYHIAGVNWGRDLPEPVVADIRNVKAGDPSPD
GKGALDICRGIEVGHVFQLGTKYSDAMGATFIDESGKAQPMVMGCYGIGITRILGAAIEQNFDDKGIVWPEAIAPFEVVL
CPMGYDRSDAVREAADKLYADLAAAGIDVILDDRGERPGVMFADWELIGVPHRLVIGERGLKDGKIEYQGRRDAEATLLP
ADSAAAAVAEKVRAALAR

Sequences:

>Translated_578_residues
MKASRFFIGTLKEAPADAEIVSHKLMVRAGMIRRVAGGIYNYLPVGLRSIRKVEAIVREEMNRAGAIELLMPAVQPAELW
QESGRWEQYGPELLRFKDRKQNEFVIGPTHEEVVTDIARNQIKSYRQMPVNFYQIQTKFRDEIRPRFGVMRGREFIMKDA
YSFDKDHESLKESYKKMYDAYVRIFTRIGLEFRPVAADNGSIGGSGSHEFHVIADTGEDAIAYCPTSDFAANVEAAEALP
LLASRAAPAEAMQKVATPGKAKCEAVAELMGIPLERTIKSIVLATDNEGAEPTIWLLMLRGDHDLNEIKTAKLPGLAGHR
FATEAEIVEWFGTPPGYLGPIGTKKPVRVVADRTVANMSDFVVGANEVDYHIAGVNWGRDLPEPVVADIRNVKAGDPSPD
GKGALDICRGIEVGHVFQLGTKYSDAMGATFIDESGKAQPMVMGCYGIGITRILGAAIEQNFDDKGIVWPEAIAPFEVVL
CPMGYDRSDAVREAADKLYADLAAAGIDVILDDRGERPGVMFADWELIGVPHRLVIGERGLKDGKIEYQGRRDAEATLLP
ADSAAAAVAEKVRAALAR
>Mature_578_residues
MKASRFFIGTLKEAPADAEIVSHKLMVRAGMIRRVAGGIYNYLPVGLRSIRKVEAIVREEMNRAGAIELLMPAVQPAELW
QESGRWEQYGPELLRFKDRKQNEFVIGPTHEEVVTDIARNQIKSYRQMPVNFYQIQTKFRDEIRPRFGVMRGREFIMKDA
YSFDKDHESLKESYKKMYDAYVRIFTRIGLEFRPVAADNGSIGGSGSHEFHVIADTGEDAIAYCPTSDFAANVEAAEALP
LLASRAAPAEAMQKVATPGKAKCEAVAELMGIPLERTIKSIVLATDNEGAEPTIWLLMLRGDHDLNEIKTAKLPGLAGHR
FATEAEIVEWFGTPPGYLGPIGTKKPVRVVADRTVANMSDFVVGANEVDYHIAGVNWGRDLPEPVVADIRNVKAGDPSPD
GKGALDICRGIEVGHVFQLGTKYSDAMGATFIDESGKAQPMVMGCYGIGITRILGAAIEQNFDDKGIVWPEAIAPFEVVL
CPMGYDRSDAVREAADKLYADLAAAGIDVILDDRGERPGVMFADWELIGVPHRLVIGERGLKDGKIEYQGRRDAEATLLP
ADSAAAAVAEKVRAALAR

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids su

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI34303926, Length=229, Percent_Identity=44.1048034934498, Blast_Score=224, Evalue=2e-58,
Organism=Escherichia coli, GI1786392, Length=574, Percent_Identity=57.3170731707317, Blast_Score=692, Evalue=0.0,
Organism=Caenorhabditis elegans, GI115532348, Length=252, Percent_Identity=38.0952380952381, Blast_Score=181, Evalue=1e-45,
Organism=Caenorhabditis elegans, GI193203271, Length=86, Percent_Identity=40.6976744186046, Blast_Score=83, Evalue=4e-16,
Organism=Saccharomyces cerevisiae, GI6320931, Length=221, Percent_Identity=39.8190045248869, Blast_Score=189, Evalue=1e-48,
Organism=Drosophila melanogaster, GI24656200, Length=245, Percent_Identity=37.9591836734694, Blast_Score=182, Evalue=6e-46,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002316
- InterPro:   IPR004500
- InterPro:   IPR007214 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; PF04073 YbaK [H]

EC number: =6.1.1.15 [H]

Molecular weight: Translated: 63420; Mature: 63420

Theoretical pI: Translated: 5.36; Mature: 5.36

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKASRFFIGTLKEAPADAEIVSHKLMVRAGMIRRVAGGIYNYLPVGLRSIRKVEAIVREE
CCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MNRAGAIELLMPAVQPAELWQESGRWEQYGPELLRFKDRKQNEFVIGPTHEEVVTDIARN
HHHCCCHHHHHCCCCHHHHHHHCCCHHHCCHHHHHHHCCCCCCEEECCCHHHHHHHHHHH
QIKSYRQMPVNFYQIQTKFRDEIRPRFGVMRGREFIMKDAYSFDKDHESLKESYKKMYDA
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
YVRIFTRIGLEFRPVAADNGSIGGSGSHEFHVIADTGEDAIAYCPTSDFAANVEAAEALP
HHHHHHHCCCEEEEEECCCCCCCCCCCCEEEEEEECCCCCEEECCCCCCCCCCHHHHHHH
LLASRAAPAEAMQKVATPGKAKCEAVAELMGIPLERTIKSIVLATDNEGAEPTIWLLMLR
HHHHCCCCHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHEEEEECCCCCCCEEEEEEEE
GDHDLNEIKTAKLPGLAGHRFATEAEIVEWFGTPPGYLGPIGTKKPVRVVADRTVANMSD
CCCCHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCEEEHHHHHHCCHH
FVVGANEVDYHIAGVNWGRDLPEPVVADIRNVKAGDPSPDGKGALDICRGIEVGHVFQLG
HEECCCCCEEEEEECCCCCCCCCHHHHHHHHCCCCCCCCCCCCHHHHHHCCCCCHHEECC
TKYSDAMGATFIDESGKAQPMVMGCYGIGITRILGAAIEQNFDDKGIVWPEAIAPFEVVL
CCHHHHCCCEEECCCCCCCCEEEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEE
CPMGYDRSDAVREAADKLYADLAAAGIDVILDDRGERPGVMFADWELIGVPHRLVIGERG
ECCCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCEEEECEEEECCCCEEEECCCC
LKDGKIEYQGRRDAEATLLPADSAAAAVAEKVRAALAR
CCCCCEEECCCCCCCEEEECCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKASRFFIGTLKEAPADAEIVSHKLMVRAGMIRRVAGGIYNYLPVGLRSIRKVEAIVREE
CCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MNRAGAIELLMPAVQPAELWQESGRWEQYGPELLRFKDRKQNEFVIGPTHEEVVTDIARN
HHHCCCHHHHHCCCCHHHHHHHCCCHHHCCHHHHHHHCCCCCCEEECCCHHHHHHHHHHH
QIKSYRQMPVNFYQIQTKFRDEIRPRFGVMRGREFIMKDAYSFDKDHESLKESYKKMYDA
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
YVRIFTRIGLEFRPVAADNGSIGGSGSHEFHVIADTGEDAIAYCPTSDFAANVEAAEALP
HHHHHHHCCCEEEEEECCCCCCCCCCCCEEEEEEECCCCCEEECCCCCCCCCCHHHHHHH
LLASRAAPAEAMQKVATPGKAKCEAVAELMGIPLERTIKSIVLATDNEGAEPTIWLLMLR
HHHHCCCCHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHEEEEECCCCCCCEEEEEEEE
GDHDLNEIKTAKLPGLAGHRFATEAEIVEWFGTPPGYLGPIGTKKPVRVVADRTVANMSD
CCCCHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCEEEHHHHHHCCHH
FVVGANEVDYHIAGVNWGRDLPEPVVADIRNVKAGDPSPDGKGALDICRGIEVGHVFQLG
HEECCCCCEEEEEECCCCCCCCCHHHHHHHHCCCCCCCCCCCCHHHHHHCCCCCHHEECC
TKYSDAMGATFIDESGKAQPMVMGCYGIGITRILGAAIEQNFDDKGIVWPEAIAPFEVVL
CCHHHHCCCEEECCCCCCCCEEEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEE
CPMGYDRSDAVREAADKLYADLAAAGIDVILDDRGERPGVMFADWELIGVPHRLVIGERG
ECCCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCEEEECEEEECCCCEEEECCCC
LKDGKIEYQGRRDAEATLLPADSAAAAVAEKVRAALAR
CCCCCEEECCCCCCCEEEECCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA