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The map label for this gene is potF [H]

Identifier: 126455252

GI number: 126455252

Start: 134836

End: 135930

Strand: Direct

Name: potF [H]

Synonym: BURPS1106A_0140

Alternate gene names: 126455252

Gene position: 134836-135930 (Clockwise)

Preceding gene: 126454673

Following gene: 126452138

Centisome position: 3.38

GC content: 65.02

Gene sequence:

>1095_bases
ATGAAGCGCATCGCGTGGCTCGCCGCCGTGCTCGCATCGCTCGCGTGCGCCGCGGCGCGCGCGGCCGACGGCAACGTGCT
CAACATCTACAATTGGGCCGAGTATTTCGCGCCCGACACGATCGCCGGCTTCGAGAAGGAAACCGGCATCAAGGTGCGGC
TCGACGTGTACGACAGCAACGAAGCGCTGCAAACGAAGCTGACGACGGGCAACAGCGGCTACGATCTCGTGTTTCCGTCA
AACGATTTTCTCGCACGGCAGATTCAGGCGGGCCTCTACCGGAAACTCGACAAATCGAGGCTGCCGAACCTCACGAACCT
CGATCCGGCGATCGTCGCGCGCGCCGCCGAAGTCGATCCCGGCAACCAGTACAGCGTGCCGTACATGCAAGGCACGTTCG
GGCTCGGGCTGAATGTCGCGAAGGTGAAGCAGGCGCTCGGCGGCCCGCTGCCCGCGAACACGCTTGAGCTGATCTTCAAT
CCCGCGTATGCGGCGAAGCTCGAGCGCTGCGGCATCGCGTTCAACGATGCGGGCAGCGAAGTGTTTCCGCTCGCGCTGCG
CTACATCGGCCGCGATCCGAACACGACCGATCCGCGCGACTATGAGGCGGCGCTCGACATGATGAAGAAGATCCGGCCGA
CGATCCGCCAGTTCATCGCGACGCCCGTGATGAACGATCTCGCGACGGGCGACGTATGCGTCGTCACCGGCTACTCGGGC
GCGGTGCTTGTCGCGGCGCGGCGCGCGGCCGAGGCGAAGAACGGGCAGCAGATCGTCTACAGCCTGCCCTCGGCCGGCGC
GCCGTTCTGGTTCGACAGCATGGCGATTCCGAAAGGTGCGGCGCACGCGGACCATGCGCTGCGCTTCATCGATTACATTC
TGAGGCCCGACGTCGTCGCGAAGATCAGCAACAAGGTGATGTACCCGAATCCGAACCGCGTGGCGACGCCGCTCGTCGAC
CGGCGGCTCACGGCCAACCCGGCGATCTATCCGGATGCGGCGACGATGCGTACGCTGTGGGTGAAGCGCCCGATGCCGCC
GCAGGCAATACGCATGCAGACGCGCTACTGGACCCGGTTCAAGACCGGTTACTGA

Upstream 100 bases:

>100_bases
AATCGATTTTTTTCTCTACTTACGATCATCGGTGGAACGTTCGCCGATGCAGCGCGCGAGCGGCCGCCCTTTTTCTTCGG
CATCGTCAAAGGAGAGATCG

Downstream 100 bases:

>100_bases
GCGGCTTCATTCTGACGAGGAGACCGAATTGATTCCCACCGGACTGCTGTTCGATCCCGCATTCCTGTCGCATCGGCAGG
GTGATCTCGTGTATGTCGTG

Product: extracellular solute-binding protein

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 364; Mature: 364

Protein sequence:

>364_residues
MKRIAWLAAVLASLACAAARAADGNVLNIYNWAEYFAPDTIAGFEKETGIKVRLDVYDSNEALQTKLTTGNSGYDLVFPS
NDFLARQIQAGLYRKLDKSRLPNLTNLDPAIVARAAEVDPGNQYSVPYMQGTFGLGLNVAKVKQALGGPLPANTLELIFN
PAYAAKLERCGIAFNDAGSEVFPLALRYIGRDPNTTDPRDYEAALDMMKKIRPTIRQFIATPVMNDLATGDVCVVTGYSG
AVLVAARRAAEAKNGQQIVYSLPSAGAPFWFDSMAIPKGAAHADHALRFIDYILRPDVVAKISNKVMYPNPNRVATPLVD
RRLTANPAIYPDAATMRTLWVKRPMPPQAIRMQTRYWTRFKTGY

Sequences:

>Translated_364_residues
MKRIAWLAAVLASLACAAARAADGNVLNIYNWAEYFAPDTIAGFEKETGIKVRLDVYDSNEALQTKLTTGNSGYDLVFPS
NDFLARQIQAGLYRKLDKSRLPNLTNLDPAIVARAAEVDPGNQYSVPYMQGTFGLGLNVAKVKQALGGPLPANTLELIFN
PAYAAKLERCGIAFNDAGSEVFPLALRYIGRDPNTTDPRDYEAALDMMKKIRPTIRQFIATPVMNDLATGDVCVVTGYSG
AVLVAARRAAEAKNGQQIVYSLPSAGAPFWFDSMAIPKGAAHADHALRFIDYILRPDVVAKISNKVMYPNPNRVATPLVD
RRLTANPAIYPDAATMRTLWVKRPMPPQAIRMQTRYWTRFKTGY
>Mature_364_residues
MKRIAWLAAVLASLACAAARAADGNVLNIYNWAEYFAPDTIAGFEKETGIKVRLDVYDSNEALQTKLTTGNSGYDLVFPS
NDFLARQIQAGLYRKLDKSRLPNLTNLDPAIVARAAEVDPGNQYSVPYMQGTFGLGLNVAKVKQALGGPLPANTLELIFN
PAYAAKLERCGIAFNDAGSEVFPLALRYIGRDPNTTDPRDYEAALDMMKKIRPTIRQFIATPVMNDLATGDVCVVTGYSG
AVLVAARRAAEAKNGQQIVYSLPSAGAPFWFDSMAIPKGAAHADHALRFIDYILRPDVVAKISNKVMYPNPNRVATPLVD
RRLTANPAIYPDAATMRTLWVKRPMPPQAIRMQTRYWTRFKTGY

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine. Polyamine binding protein [H]

COG id: COG0687

COG function: function code E; Spermidine/putrescine-binding periplasmic protein

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein potD/potF family [H]

Homologues:

Organism=Escherichia coli, GI1787078, Length=362, Percent_Identity=47.2375690607735, Blast_Score=347, Evalue=7e-97,
Organism=Escherichia coli, GI1787367, Length=324, Percent_Identity=36.4197530864198, Blast_Score=206, Evalue=3e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR001188 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 39996; Mature: 39996

Theoretical pI: Translated: 9.71; Mature: 9.71

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRIAWLAAVLASLACAAARAADGNVLNIYNWAEYFAPDTIAGFEKETGIKVRLDVYDSN
CCHHHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHCCCHHCCCCCCCCCEEEEEEECCC
EALQTKLTTGNSGYDLVFPSNDFLARQIQAGLYRKLDKSRLPNLTNLDPAIVARAAEVDP
CCEEEEEECCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHEEHHCCCC
GNQYSVPYMQGTFGLGLNVAKVKQALGGPLPANTLELIFNPAYAAKLERCGIAFNDAGSE
CCCCCCCEECCCCCCCCCHHHHHHHHCCCCCCCHHHEEECCHHHHHHHHCCEEECCCCCH
VFPLALRYIGRDPNTTDPRDYEAALDMMKKIRPTIRQFIATPVMNDLATGDVCVVTGYSG
HHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCC
AVLVAARRAAEAKNGQQIVYSLPSAGAPFWFDSMAIPKGAAHADHALRFIDYILRPDVVA
EEEEHHHHHHHCCCCCEEEEECCCCCCCCEECCCCCCCCCCHHHHHHHHHHHHHCCHHHH
KISNKVMYPNPNRVATPLVDRRLTANPAIYPDAATMRTLWVKRPMPPQAIRMQTRYWTRF
HHHCCEECCCCCCCCCHHHHCCCCCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHC
KTGY
CCCC
>Mature Secondary Structure
MKRIAWLAAVLASLACAAARAADGNVLNIYNWAEYFAPDTIAGFEKETGIKVRLDVYDSN
CCHHHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHCCCHHCCCCCCCCCEEEEEEECCC
EALQTKLTTGNSGYDLVFPSNDFLARQIQAGLYRKLDKSRLPNLTNLDPAIVARAAEVDP
CCEEEEEECCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHEEHHCCCC
GNQYSVPYMQGTFGLGLNVAKVKQALGGPLPANTLELIFNPAYAAKLERCGIAFNDAGSE
CCCCCCCEECCCCCCCCCHHHHHHHHCCCCCCCHHHEEECCHHHHHHHHCCEEECCCCCH
VFPLALRYIGRDPNTTDPRDYEAALDMMKKIRPTIRQFIATPVMNDLATGDVCVVTGYSG
HHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCC
AVLVAARRAAEAKNGQQIVYSLPSAGAPFWFDSMAIPKGAAHADHALRFIDYILRPDVVA
EEEEHHHHHHHCCCCCEEEEECCCCCCCCEECCCCCCCCCCHHHHHHHHHHHHHCCHHHH
KISNKVMYPNPNRVATPLVDRRLTANPAIYPDAATMRTLWVKRPMPPQAIRMQTRYWTRF
HHHCCEECCCCCCCCCHHHHCCCCCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHC
KTGY
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: Polyamine Binding Protein [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8416922; 8905232; 9278503; 9298646; 9651355 [H]