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The map label for this gene is emrB [H]

Identifier: 126455044

GI number: 126455044

Start: 23442

End: 25004

Strand: Direct

Name: emrB [H]

Synonym: BURPS1106A_0023

Alternate gene names: 126455044

Gene position: 23442-25004 (Clockwise)

Preceding gene: 126454172

Following gene: 126452971

Centisome position: 0.59

GC content: 66.79

Gene sequence:

>1563_bases
ATGGCTGCGACGGCCCCCGCTTCCCCTTCCCGCTCCGCCGAGCCGGCGCCGCTGTCGGGCGGCACGCTCGCGCTGCTGAC
GATCGGGCTCGCGCTCGGCACGTTCATGGAGGTGCTCGACACGTCGATCGCGAATGTCGCGGTGCCGACGATCTCCGGCA
GCCTCGGCGTCGCGACGAGCGAAGGCACGTGGGTGATCTCGTCGTATTCGGTCGCCTCCGCGATCGCGGTGCCGCTGACC
GGCTGGCTCGCGCGGCGGGTCGGCGAGGTGCGGCTGTTCACGCTGTCGGTGCTCGCGTTCACGATCGCGTCCGCGCTCTG
CGGCCTCGCGGAGAACTTCGAGACGCTGATCGCGTTCCGGCTGTTGCAGGGGCTCGTGTCGGGGCCGATGGTGCCGCTGT
CGCAGACGATCCTGATGCGCAGCTATCCGCCCGCGAAGCGCGGGCTCGCGCTCGGCCTGTGGGCGATGACGGTGATCGTC
GCGCCGATCTTCGGCCCGCTGCTCGGCGGCTGGATCAGCGACAACTACACGTGGCCGTGGATCTTCTATATCAACCTGCC
GATCGGCGTGTTCTCCGCCGCGTGCGCGTTCTTCCTGTTGCGCGGCCGCGAGACGAAGACGACGAAGCAGCGGATCGACG
CGATCGGGCTCGCGCTGCTCGTGATCGGCGTGTCGTGCCTGCAGATGATGCTCGACCTCGGCAAGGATCGCGACTGGTTC
AACTCGACGTTCATCACCTCGCTCGCGCTGATCGCCGTCGTGTCGCTCGCGTTCATGCTCGTGTGGGAATCCACCGAGAA
GGAGCCGGTCGTCGACCTGTCGCTCTTCAAGGACCGCAACTTCGCGCTCGGCGCGATGATCATCTCGTTCGGCTTCATGG
CGTTCTTCGGCTCGGTCGTGATCTTTCCGCTGTGGCTGCAGACCGTGATGGGCTACACGGCGGGCCTCGCCGGCCTCGCC
ACGGCGCCCGTCGGCATCCTCGCGCTCGTGCTCTCGCCGATGATCGGCCGCAACATGCACCGGCTCGATCTGCGGATGGT
CGCGAGCTTCGCGTTCGTCGTGTTCGCCGTCGTGTCGATCTGGAATTCGATGTTTACGCTCGACGTGCCGTTCAACCATG
TGATCCTGCCGCGGCTCGTGCAGGGCATCGGCGTCGCGTGCTTTTTCGTGCCGATGACGACGATCACGCTCTCCAGCATT
CCCGACGAGCGGCTCGCGAGCGCGTCGGGGCTGTCGAACTTCCTGCGTACGCTGTCGGGCGCGATCGGCACCGCGGTGAG
CTCGACGTTCTGGGAAAACGACGCGATCTATCACCACGCGCGGCTCGCCGAATCGGTGAACGTGTATGCGCAGAGCACGC
TCGACTATCAAGGCGCGCTCGCGCGGCTCGGCGTGATGGGCGACGTGTCGACCGCGCAGATCAACCAGATCGTCACGCAG
CAGGGCTTCATGATGGCGACCAACGACTTCTTCCACATTTCGGCGCTCGCGTTCGTCGCGCTCGCGGCGCTCGTGTGGGT
GACGAAGCCGAAGAAAGGGGCCGGGCCCGCGATCGGGCACTGA

Upstream 100 bases:

>100_bases
TATTTATCTGTCTGGGCAGAAGCTGACAGGACACTTACTGTCGACCGATGCCGATGCACGAAAGGATGCCGGGGCAACAG
TCGCCCCCTAGGAGAGACAC

Downstream 100 bases:

>100_bases
GCGGCGCTCGGCCCGGCCCGGTCCGGGCATGGGGCGCGCGGCGCGCCCGGGCCAAGCGCTCAGCGCGCGCGCAGGCTGCC
GAACGCGAGATCGTCGCCGG

Product: EmrB/QacA family drug resistance transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 520; Mature: 519

Protein sequence:

>520_residues
MAATAPASPSRSAEPAPLSGGTLALLTIGLALGTFMEVLDTSIANVAVPTISGSLGVATSEGTWVISSYSVASAIAVPLT
GWLARRVGEVRLFTLSVLAFTIASALCGLAENFETLIAFRLLQGLVSGPMVPLSQTILMRSYPPAKRGLALGLWAMTVIV
APIFGPLLGGWISDNYTWPWIFYINLPIGVFSAACAFFLLRGRETKTTKQRIDAIGLALLVIGVSCLQMMLDLGKDRDWF
NSTFITSLALIAVVSLAFMLVWESTEKEPVVDLSLFKDRNFALGAMIISFGFMAFFGSVVIFPLWLQTVMGYTAGLAGLA
TAPVGILALVLSPMIGRNMHRLDLRMVASFAFVVFAVVSIWNSMFTLDVPFNHVILPRLVQGIGVACFFVPMTTITLSSI
PDERLASASGLSNFLRTLSGAIGTAVSSTFWENDAIYHHARLAESVNVYAQSTLDYQGALARLGVMGDVSTAQINQIVTQ
QGFMMATNDFFHISALAFVALAALVWVTKPKKGAGPAIGH

Sequences:

>Translated_520_residues
MAATAPASPSRSAEPAPLSGGTLALLTIGLALGTFMEVLDTSIANVAVPTISGSLGVATSEGTWVISSYSVASAIAVPLT
GWLARRVGEVRLFTLSVLAFTIASALCGLAENFETLIAFRLLQGLVSGPMVPLSQTILMRSYPPAKRGLALGLWAMTVIV
APIFGPLLGGWISDNYTWPWIFYINLPIGVFSAACAFFLLRGRETKTTKQRIDAIGLALLVIGVSCLQMMLDLGKDRDWF
NSTFITSLALIAVVSLAFMLVWESTEKEPVVDLSLFKDRNFALGAMIISFGFMAFFGSVVIFPLWLQTVMGYTAGLAGLA
TAPVGILALVLSPMIGRNMHRLDLRMVASFAFVVFAVVSIWNSMFTLDVPFNHVILPRLVQGIGVACFFVPMTTITLSSI
PDERLASASGLSNFLRTLSGAIGTAVSSTFWENDAIYHHARLAESVNVYAQSTLDYQGALARLGVMGDVSTAQINQIVTQ
QGFMMATNDFFHISALAFVALAALVWVTKPKKGAGPAIGH
>Mature_519_residues
AATAPASPSRSAEPAPLSGGTLALLTIGLALGTFMEVLDTSIANVAVPTISGSLGVATSEGTWVISSYSVASAIAVPLTG
WLARRVGEVRLFTLSVLAFTIASALCGLAENFETLIAFRLLQGLVSGPMVPLSQTILMRSYPPAKRGLALGLWAMTVIVA
PIFGPLLGGWISDNYTWPWIFYINLPIGVFSAACAFFLLRGRETKTTKQRIDAIGLALLVIGVSCLQMMLDLGKDRDWFN
STFITSLALIAVVSLAFMLVWESTEKEPVVDLSLFKDRNFALGAMIISFGFMAFFGSVVIFPLWLQTVMGYTAGLAGLAT
APVGILALVLSPMIGRNMHRLDLRMVASFAFVVFAVVSIWNSMFTLDVPFNHVILPRLVQGIGVACFFVPMTTITLSSIP
DERLASASGLSNFLRTLSGAIGTAVSSTFWENDAIYHHARLAESVNVYAQSTLDYQGALARLGVMGDVSTAQINQIVTQQ
GFMMATNDFFHISALAFVALAALVWVTKPKKGAGPAIGH

Specific function: Translocase that confers resistance to substances of high hydrophobicity [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=498, Percent_Identity=53.2128514056225, Blast_Score=526, Evalue=1e-150,
Organism=Escherichia coli, GI1788710, Length=515, Percent_Identity=47.9611650485437, Blast_Score=500, Evalue=1e-143,
Organism=Escherichia coli, GI1790195, Length=414, Percent_Identity=31.4009661835749, Blast_Score=172, Evalue=5e-44,
Organism=Escherichia coli, GI1788392, Length=411, Percent_Identity=30.1703163017032, Blast_Score=130, Evalue=2e-31,
Organism=Escherichia coli, GI87081983, Length=426, Percent_Identity=25.3521126760563, Blast_Score=125, Evalue=8e-30,
Organism=Saccharomyces cerevisiae, GI6322958, Length=208, Percent_Identity=30.7692307692308, Blast_Score=79, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6325455, Length=129, Percent_Identity=30.2325581395349, Blast_Score=74, Evalue=8e-14,
Organism=Saccharomyces cerevisiae, GI6321663, Length=150, Percent_Identity=28, Blast_Score=69, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 55539; Mature: 55408

Theoretical pI: Translated: 7.98; Mature: 7.98

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAATAPASPSRSAEPAPLSGGTLALLTIGLALGTFMEVLDTSIANVAVPTISGSLGVATS
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCEEC
EGTWVISSYSVASAIAVPLTGWLARRVGEVRLFTLSVLAFTIASALCGLAENFETLIAFR
CCCEEEECHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLQGLVSGPMVPLSQTILMRSYPPAKRGLALGLWAMTVIVAPIFGPLLGGWISDNYTWPW
HHHHHHCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCEE
IFYINLPIGVFSAACAFFLLRGRETKTTKQRIDAIGLALLVIGVSCLQMMLDLGKDRDWF
EEEEECCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHH
NSTFITSLALIAVVSLAFMLVWESTEKEPVVDLSLFKDRNFALGAMIISFGFMAFFGSVV
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEHHHHCCCCHHHHHHHHHHHHHHHHHHHH
IFPLWLQTVMGYTAGLAGLATAPVGILALVLSPMIGRNMHRLDLRMVASFAFVVFAVVSI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
WNSMFTLDVPFNHVILPRLVQGIGVACFFVPMTTITLSSIPDERLASASGLSNFLRTLSG
HHHHEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
AIGTAVSSTFWENDAIYHHARLAESVNVYAQSTLDYQGALARLGVMGDVSTAQINQIVTQ
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCHHHHHHHHHHH
QGFMMATNDFFHISALAFVALAALVWVTKPKKGAGPAIGH
CCCEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
AATAPASPSRSAEPAPLSGGTLALLTIGLALGTFMEVLDTSIANVAVPTISGSLGVATS
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCEEC
EGTWVISSYSVASAIAVPLTGWLARRVGEVRLFTLSVLAFTIASALCGLAENFETLIAFR
CCCEEEECHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLQGLVSGPMVPLSQTILMRSYPPAKRGLALGLWAMTVIVAPIFGPLLGGWISDNYTWPW
HHHHHHCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCEE
IFYINLPIGVFSAACAFFLLRGRETKTTKQRIDAIGLALLVIGVSCLQMMLDLGKDRDWF
EEEEECCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHH
NSTFITSLALIAVVSLAFMLVWESTEKEPVVDLSLFKDRNFALGAMIISFGFMAFFGSVV
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEHHHHCCCCHHHHHHHHHHHHHHHHHHHH
IFPLWLQTVMGYTAGLAGLATAPVGILALVLSPMIGRNMHRLDLRMVASFAFVVFAVVSI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
WNSMFTLDVPFNHVILPRLVQGIGVACFFVPMTTITLSSIPDERLASASGLSNFLRTLSG
HHHHEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
AIGTAVSSTFWENDAIYHHARLAESVNVYAQSTLDYQGALARLGVMGDVSTAQINQIVTQ
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCHHHHHHHHHHH
QGFMMATNDFFHISALAFVALAALVWVTKPKKGAGPAIGH
CCCEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]