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The map label for this gene is pnp [H]

Identifier: 126454918

GI number: 126454918

Start: 1266050

End: 1268191

Strand: Direct

Name: pnp [H]

Synonym: BURPS1106A_1294

Alternate gene names: 126454918

Gene position: 1266050-1268191 (Clockwise)

Preceding gene: 126451731

Following gene: 126453705

Centisome position: 31.74

GC content: 66.01

Gene sequence:

>2142_bases
ATGTCTCTGTTCAATAAAATCGTGAAGGAATTCCAGTGGGGCCAGCACAAGGTGCGCCTCGAAACCGGTGAAATCGCGCG
CCAGGCAAGCGGCGCCGTGATCGTCGACATCGAGGACACCGTCGTGCTCGCGACCGTCGTCGGCGCGAAGTCGGCGAAGC
CGGGCCAGGATTTCTTCCCGCTCACCGTCGACTACATCGAGAAGACCTACTCGGCCGGCAAGATCCCGGGCGGCTTCTTC
CGCCGCGAAGGCCGTCCGTCGGAGCACGAAACGCTGACGTCGCGCCTCATCGACCGCCCGCTGCGCCCGCTCTTCCCCGA
AGGCTTCTATAACGAAGTGCAGGTCGTGATCCACGTGCTGTCCGTGAACCCGGAGATTCCGGCGGACATCCCGGCACTCA
TCGGCGCATCGGCTGCGCTCGCCGTGTCCGGTCTGCCGTTCAACGGCCCGGTCGGCGCCGCGCGCGTCGCGTACGTGAAC
AACGAATATGTGCTGAATCCGACGCGTGAGCAGATCAAGGCGTCGCGCCTCGATCTCGTCGTCGCGGGCACGGAGCGTGC
GGTGCTGATGGTCGAGTCGGAAGCCGACCAACTGCCGGAGGACGTGATGCTCGGCGCGGTGGTGTTCGGCCACGAGCAGA
TGCAGACGGCGATCGACGCGATCCACGAGCTCGTGCGCGAAGGCGGCAAGCCCGAGTGGGACTGGCAGCCGGCGCCGAAG
GACGAGGCGCTGAACGCGCGCGTGACCGAACTCGCGCAGCCGGAGTTGCTCGCCGCCTACCAGATCCGCGACAAGCAGGC
GCGCTCGACGAAGCTGAAGGAAGTGTACGCGGCGACGTCGGCGAAGCTGGAGGAAGAAGCGGTGGCGGCCGGCACGGTCG
CGGCGGACAAGGCGACCGTCGGCAACATCCTGTTCGACCTCGAGGCGAAGATCGTTCGCGGCCAGATCCTGAACGGCGAG
CCGCGCATCGACGGCCGCGACACGCGCACGGTGCGCCCGATCGAAATCCGCACGGGCGTGCTGCCGCGCACCCACGGCTC
GGCGCTGTTCACGCGCGGCGAGACGCAGGCGCTCGTCGTCGCGACGCTCGGCACGAAGGGTGACGAACAGATCATCGACG
CGCTCGAAGGCGAATACCGCGAGCGCTTCATGCTCCACTACAACATGCCCCCGTTCGCGACCGGCGAAACGGGCCGCGTC
GGCTCGCCGAAGCGCCGTGAGATCGGCCACGGCCGGCTCGCGAAGCGCGCGCTCGTCGCGTGCCTGCCGAGCGCCGACGA
ATTCGGCTACTCGATTCGCGTCGTGTCGGAAATCACCGAATCGAACGGTTCGTCGTCGATGGCGTCGGTGTGCGGCGGCT
GCCTCGCGCTGATGGACGCCGGCGTGCCGATGAAGGCGCACGTCGCGGGCATCGCGATGGGCCTGATCCTCGAGGGCAAC
AAGTTCGCGGTGCTGACCGATATCCTCGGCGACGAGGACCACCTCGGCGACATGGACTTCAAGGTGGCCGGCACGGCGGA
CGGCGTGACCGCGCTGCAGATGGACATCAAGATCCAGGGCATCACGAAGGAAATCATGCAGGTCGCGCTCGCGCAGGCGA
AGGAAGGCCGCATGCACATCCTCGGCAAGATGAAGGACGCGGTCGCGGGCGCGAACACGCAACTGTCCGAATTCGCGCCG
CGCATGATCACGATCAAGATCAACCCGGAAAAGATTCGCGACGTGATCGGCAAGGGCGGCTCGGTGATCCGCGCGCTGAC
CGAAGAAACCGGCACGACGATCGACATCTCGGATGACGGCGTCGTGACGATCGCGAGCACGAACAGCGAAGGGATGGCCG
AGGCGAAGAAGCGCATCGAGAACATCACGGCCGAGATCGAAGTCGGCCACGTGTACGAAGGCACGGTGCTCAAGCTGCTC
GATTTCGGCGCGATCGTGAACCTGCTGCCGGGCAAGGACGGCCTGCTGCACATCTCGGAAATCGTCAACGAGCGCGTGAA
GGACATCAACGACTACCTGAAGGAAGGCCAGCAGGTGAAGGTCAAGGTGATCCAGACGGACGAGAAGGGGCGCGTGCGTC
TGTCGGCGAAGGCGCTGCTGAACGAGGCGGCCGCCCAGGCGGATACGCCGCCGCAGCAGTAA

Upstream 100 bases:

>100_bases
GCATAAAAAACACACCTCTTCCCATGTGGCCCGGTCGCGCCGCCGCCGTGCCGGCCTCGTCCGCGCACGGCGTCCCGACA
ACGAACGAAAAGGAGCGACC

Downstream 100 bases:

>100_bases
GCGGCAAGGCGACGGCCGGCAGCGCGAATGCGCGCCGGCCGTTTTTCACAGGATGGGCCGGTTTGCGTGGCGCGCGTCGC
GCGCGCAAACCCGTCACGAT

Product: polynucleotide phosphorylase/polyadenylase

Products: NA

Alternate protein names: Polynucleotide phosphorylase; PNPase [H]

Number of amino acids: Translated: 713; Mature: 712

Protein sequence:

>713_residues
MSLFNKIVKEFQWGQHKVRLETGEIARQASGAVIVDIEDTVVLATVVGAKSAKPGQDFFPLTVDYIEKTYSAGKIPGGFF
RREGRPSEHETLTSRLIDRPLRPLFPEGFYNEVQVVIHVLSVNPEIPADIPALIGASAALAVSGLPFNGPVGAARVAYVN
NEYVLNPTREQIKASRLDLVVAGTERAVLMVESEADQLPEDVMLGAVVFGHEQMQTAIDAIHELVREGGKPEWDWQPAPK
DEALNARVTELAQPELLAAYQIRDKQARSTKLKEVYAATSAKLEEEAVAAGTVAADKATVGNILFDLEAKIVRGQILNGE
PRIDGRDTRTVRPIEIRTGVLPRTHGSALFTRGETQALVVATLGTKGDEQIIDALEGEYRERFMLHYNMPPFATGETGRV
GSPKRREIGHGRLAKRALVACLPSADEFGYSIRVVSEITESNGSSSMASVCGGCLALMDAGVPMKAHVAGIAMGLILEGN
KFAVLTDILGDEDHLGDMDFKVAGTADGVTALQMDIKIQGITKEIMQVALAQAKEGRMHILGKMKDAVAGANTQLSEFAP
RMITIKINPEKIRDVIGKGGSVIRALTEETGTTIDISDDGVVTIASTNSEGMAEAKKRIENITAEIEVGHVYEGTVLKLL
DFGAIVNLLPGKDGLLHISEIVNERVKDINDYLKEGQQVKVKVIQTDEKGRVRLSAKALLNEAAAQADTPPQQ

Sequences:

>Translated_713_residues
MSLFNKIVKEFQWGQHKVRLETGEIARQASGAVIVDIEDTVVLATVVGAKSAKPGQDFFPLTVDYIEKTYSAGKIPGGFF
RREGRPSEHETLTSRLIDRPLRPLFPEGFYNEVQVVIHVLSVNPEIPADIPALIGASAALAVSGLPFNGPVGAARVAYVN
NEYVLNPTREQIKASRLDLVVAGTERAVLMVESEADQLPEDVMLGAVVFGHEQMQTAIDAIHELVREGGKPEWDWQPAPK
DEALNARVTELAQPELLAAYQIRDKQARSTKLKEVYAATSAKLEEEAVAAGTVAADKATVGNILFDLEAKIVRGQILNGE
PRIDGRDTRTVRPIEIRTGVLPRTHGSALFTRGETQALVVATLGTKGDEQIIDALEGEYRERFMLHYNMPPFATGETGRV
GSPKRREIGHGRLAKRALVACLPSADEFGYSIRVVSEITESNGSSSMASVCGGCLALMDAGVPMKAHVAGIAMGLILEGN
KFAVLTDILGDEDHLGDMDFKVAGTADGVTALQMDIKIQGITKEIMQVALAQAKEGRMHILGKMKDAVAGANTQLSEFAP
RMITIKINPEKIRDVIGKGGSVIRALTEETGTTIDISDDGVVTIASTNSEGMAEAKKRIENITAEIEVGHVYEGTVLKLL
DFGAIVNLLPGKDGLLHISEIVNERVKDINDYLKEGQQVKVKVIQTDEKGRVRLSAKALLNEAAAQADTPPQQ
>Mature_712_residues
SLFNKIVKEFQWGQHKVRLETGEIARQASGAVIVDIEDTVVLATVVGAKSAKPGQDFFPLTVDYIEKTYSAGKIPGGFFR
REGRPSEHETLTSRLIDRPLRPLFPEGFYNEVQVVIHVLSVNPEIPADIPALIGASAALAVSGLPFNGPVGAARVAYVNN
EYVLNPTREQIKASRLDLVVAGTERAVLMVESEADQLPEDVMLGAVVFGHEQMQTAIDAIHELVREGGKPEWDWQPAPKD
EALNARVTELAQPELLAAYQIRDKQARSTKLKEVYAATSAKLEEEAVAAGTVAADKATVGNILFDLEAKIVRGQILNGEP
RIDGRDTRTVRPIEIRTGVLPRTHGSALFTRGETQALVVATLGTKGDEQIIDALEGEYRERFMLHYNMPPFATGETGRVG
SPKRREIGHGRLAKRALVACLPSADEFGYSIRVVSEITESNGSSSMASVCGGCLALMDAGVPMKAHVAGIAMGLILEGNK
FAVLTDILGDEDHLGDMDFKVAGTADGVTALQMDIKIQGITKEIMQVALAQAKEGRMHILGKMKDAVAGANTQLSEFAPR
MITIKINPEKIRDVIGKGGSVIRALTEETGTTIDISDDGVVTIASTNSEGMAEAKKRIENITAEIEVGHVYEGTVLKLLD
FGAIVNLLPGKDGLLHISEIVNERVKDINDYLKEGQQVKVKVIQTDEKGRVRLSAKALLNEAAAQADTPPQQ

Specific function: Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction [H]

COG id: COG1185

COG function: function code J; Polyribonucleotide nucleotidyltransferase (polynucleotide phosphorylase)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Homo sapiens, GI188528628, Length=718, Percent_Identity=37.883008356546, Blast_Score=439, Evalue=1e-123,
Organism=Escherichia coli, GI145693187, Length=697, Percent_Identity=65.1362984218078, Blast_Score=932, Evalue=0.0,
Organism=Caenorhabditis elegans, GI115534063, Length=735, Percent_Identity=33.1972789115646, Blast_Score=328, Evalue=7e-90,
Organism=Drosophila melanogaster, GI281362905, Length=725, Percent_Identity=35.5862068965517, Blast_Score=411, Evalue=1e-115,
Organism=Drosophila melanogaster, GI24651641, Length=725, Percent_Identity=35.5862068965517, Blast_Score=411, Evalue=1e-115,
Organism=Drosophila melanogaster, GI24651643, Length=725, Percent_Identity=35.5862068965517, Blast_Score=411, Evalue=1e-115,
Organism=Drosophila melanogaster, GI161079377, Length=676, Percent_Identity=35.5029585798817, Blast_Score=378, Evalue=1e-105,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1000 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 3328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR004087
- InterPro:   IPR004088
- InterPro:   IPR018111
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR012162
- InterPro:   IPR015848
- InterPro:   IPR003029
- InterPro:   IPR020568
- InterPro:   IPR022967 [H]

Pfam domain/function: PF00013 KH_1; PF03726 PNPase; PF01138 RNase_PH; PF03725 RNase_PH_C; PF00575 S1 [H]

EC number: =2.7.7.8 [H]

Molecular weight: Translated: 76991; Mature: 76860

Theoretical pI: Translated: 5.18; Mature: 5.18

Prosite motif: PS50084 KH_TYPE_1 ; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLFNKIVKEFQWGQHKVRLETGEIARQASGAVIVDIEDTVVLATVVGAKSAKPGQDFFP
CCHHHHHHHHHCCCCEEEEEEHHHHHHHCCCEEEEEECCCEEEEEECCCCCCCCCCCCCC
LTVDYIEKTYSAGKIPGGFFRREGRPSEHETLTSRLIDRPLRPLFPEGFYNEVQVVIHVL
CCHHHHHHHHCCCCCCCHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHEEEE
SVNPEIPADIPALIGASAALAVSGLPFNGPVGAARVAYVNNEYVLNPTREQIKASRLDLV
ECCCCCCCCCHHHHCCCHHHEEECCCCCCCCCCEEEEEECCCEEECCCHHHHHHHHEEEE
VAGTERAVLMVESEADQLPEDVMLGAVVFGHEQMQTAIDAIHELVREGGKPEWDWQPAPK
EECCCEEEEEEECCHHHCCHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
DEALNARVTELAQPELLAAYQIRDKQARSTKLKEVYAATSAKLEEEAVAAGTVAADKATV
CCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHH
GNILFDLEAKIVRGQILNGEPRIDGRDTRTVRPIEIRTGVLPRTHGSALFTRGETQALVV
HHEEEEEHHEEEECEEECCCCCCCCCCCCEEEEEEEECCCCCCCCCCEEEECCCCCEEEE
ATLGTKGDEQIIDALEGEYRERFMLHYNMPPFATGETGRVGSPKRREIGHGRLAKRALVA
EEECCCCHHHHHHHHCCHHHEEEEEEECCCCCCCCCCCCCCCCHHHHCCCHHHHHHHHHH
CLPSADEFGYSIRVVSEITESNGSSSMASVCGGCLALMDAGVPMKAHVAGIAMGLILEGN
HCCCCHHCCEEEEEEEHHHHCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHEEEEEEEECC
KFAVLTDILGDEDHLGDMDFKVAGTADGVTALQMDIKIQGITKEIMQVALAQAKEGRMHI
CEEEEEECCCCCCCCCCCCEEEECCCCCEEEEEEEEEEEHHHHHHHHHHHHHHCCCCEEE
LGKMKDAVAGANTQLSEFAPRMITIKINPEKIRDVIGKGGSVIRALTEETGTTIDISDDG
HHHHHHHHCCCCCCHHHCCCEEEEEEECHHHHHHHHCCCCHHHHHHHHCCCCEEEECCCC
VVTIASTNSEGMAEAKKRIENITAEIEVGHVYEGTVLKLLDFGAIVNLLPGKDGLLHISE
EEEEEECCCCHHHHHHHHHHHCEEEEEECEEECCHHHHHHHHHHHHHHCCCCCCCCHHHH
IVNERVKDINDYLKEGQQVKVKVIQTDEKGRVRLSAKALLNEAAAQADTPPQQ
HHHHHHHHHHHHHHCCCEEEEEEEEECCCCCEEEHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
SLFNKIVKEFQWGQHKVRLETGEIARQASGAVIVDIEDTVVLATVVGAKSAKPGQDFFP
CHHHHHHHHHCCCCEEEEEEHHHHHHHCCCEEEEEECCCEEEEEECCCCCCCCCCCCCC
LTVDYIEKTYSAGKIPGGFFRREGRPSEHETLTSRLIDRPLRPLFPEGFYNEVQVVIHVL
CCHHHHHHHHCCCCCCCHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHEEEE
SVNPEIPADIPALIGASAALAVSGLPFNGPVGAARVAYVNNEYVLNPTREQIKASRLDLV
ECCCCCCCCCHHHHCCCHHHEEECCCCCCCCCCEEEEEECCCEEECCCHHHHHHHHEEEE
VAGTERAVLMVESEADQLPEDVMLGAVVFGHEQMQTAIDAIHELVREGGKPEWDWQPAPK
EECCCEEEEEEECCHHHCCHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
DEALNARVTELAQPELLAAYQIRDKQARSTKLKEVYAATSAKLEEEAVAAGTVAADKATV
CCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHH
GNILFDLEAKIVRGQILNGEPRIDGRDTRTVRPIEIRTGVLPRTHGSALFTRGETQALVV
HHEEEEEHHEEEECEEECCCCCCCCCCCCEEEEEEEECCCCCCCCCCEEEECCCCCEEEE
ATLGTKGDEQIIDALEGEYRERFMLHYNMPPFATGETGRVGSPKRREIGHGRLAKRALVA
EEECCCCHHHHHHHHCCHHHEEEEEEECCCCCCCCCCCCCCCCHHHHCCCHHHHHHHHHH
CLPSADEFGYSIRVVSEITESNGSSSMASVCGGCLALMDAGVPMKAHVAGIAMGLILEGN
HCCCCHHCCEEEEEEEHHHHCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHEEEEEEEECC
KFAVLTDILGDEDHLGDMDFKVAGTADGVTALQMDIKIQGITKEIMQVALAQAKEGRMHI
CEEEEEECCCCCCCCCCCCEEEECCCCCEEEEEEEEEEEHHHHHHHHHHHHHHCCCCEEE
LGKMKDAVAGANTQLSEFAPRMITIKINPEKIRDVIGKGGSVIRALTEETGTTIDISDDG
HHHHHHHHCCCCCCHHHCCCEEEEEEECHHHHHHHHCCCCHHHHHHHHCCCCEEEECCCC
VVTIASTNSEGMAEAKKRIENITAEIEVGHVYEGTVLKLLDFGAIVNLLPGKDGLLHISE
EEEEEECCCCHHHHHHHHHHHCEEEEEECEEECCHHHHHHHHHHHHHHCCCCCCCCHHHH
IVNERVKDINDYLKEGQQVKVKVIQTDEKGRVRLSAKALLNEAAAQADTPPQQ
HHHHHHHHHHHHHHCCCEEEEEEEEECCCCCEEEHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA