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The map label for this gene is maiA [H]

Identifier: 126454762

GI number: 126454762

Start: 547542

End: 548186

Strand: Direct

Name: maiA [H]

Synonym: BURPS1106A_0575

Alternate gene names: 126454762

Gene position: 547542-548186 (Clockwise)

Preceding gene: 126451682

Following gene: 126453999

Centisome position: 13.73

GC content: 66.51

Gene sequence:

>645_bases
ATGAAGCTATATAGCTATTTCCGCAGCTCGGCGGCGTTTCGCGTGCGCATCGCGCTGCACCTGAAGAAACTGCCGTTCGA
CTACGTGCCCGTGCACCTGCTGCGCGACGGCGGCCAGCAACTGACGGACGACTATCGCGCGCTCAACCCCGACGCGCTCG
TGCCGACGCTCGTCGACGGCGATGCGCCGCTGCAGCAGTCGCTCGCGATCGTCGAGTATCTCGACGAAGCGTATCCGGCG
GTGCCGCTGCTGCCGCAGGCGCCCGTCGATCGCGCGTACGTGCGCGCGATCGCGCTGCAGGTCGCCTGCGAGATCCATCC
GCTCAACAACCTGCGCGTGCTGAAGTATCTGAAGCACACGCTGAAGGTGGACGACGACGCGAAGGACGCGTGGTACCGCC
ACTGGATCGAGGACGGCTTCAAATCGCTCGAGGCGCGCCTGTCGGGCGATCCGCGCACGGGCAAGCTGTGCTTCGGCGAT
ACGCCGACGCTTGCCGATCTGTGCATCGTGCCGCAGGTGTTCAACGCGCAGCGCTTCTCGATCGGCCTCGAGCGGTTTCC
GACGATTCGGCGCATCCACGATCATGCGATGACGCTCGACGCGTTCAAGGCGGCGGCGCCCGCCGCGCAGCCGGACGCCG
AGTGA

Upstream 100 bases:

>100_bases
GGCGCGGGCGGCTTTTCGCCCGTCGGCTTCGCGGCGAGCGCGGCCTGCCGCGCCCGAACGCCGGCGAGAAGATCGACGAT
CGAGACAATGAAGGACACCG

Downstream 100 bases:

>100_bases
GCGCGTCGGCCGCGCGCCGACGAAGAAAACGGGCGCCTTGCCGAACCGCGCCGCCCGCCGCCGGACATCGAATCCGGCAC
GGGGCGTGCGGCTCGCGCAG

Product: maleylacetoacetate isomerase

Products: NA

Alternate protein names: MAAI [H]

Number of amino acids: Translated: 214; Mature: 214

Protein sequence:

>214_residues
MKLYSYFRSSAAFRVRIALHLKKLPFDYVPVHLLRDGGQQLTDDYRALNPDALVPTLVDGDAPLQQSLAIVEYLDEAYPA
VPLLPQAPVDRAYVRAIALQVACEIHPLNNLRVLKYLKHTLKVDDDAKDAWYRHWIEDGFKSLEARLSGDPRTGKLCFGD
TPTLADLCIVPQVFNAQRFSIGLERFPTIRRIHDHAMTLDAFKAAAPAAQPDAE

Sequences:

>Translated_214_residues
MKLYSYFRSSAAFRVRIALHLKKLPFDYVPVHLLRDGGQQLTDDYRALNPDALVPTLVDGDAPLQQSLAIVEYLDEAYPA
VPLLPQAPVDRAYVRAIALQVACEIHPLNNLRVLKYLKHTLKVDDDAKDAWYRHWIEDGFKSLEARLSGDPRTGKLCFGD
TPTLADLCIVPQVFNAQRFSIGLERFPTIRRIHDHAMTLDAFKAAAPAAQPDAE
>Mature_214_residues
MKLYSYFRSSAAFRVRIALHLKKLPFDYVPVHLLRDGGQQLTDDYRALNPDALVPTLVDGDAPLQQSLAIVEYLDEAYPA
VPLLPQAPVDRAYVRAIALQVACEIHPLNNLRVLKYLKHTLKVDDDAKDAWYRHWIEDGFKSLEARLSGDPRTGKLCFGD
TPTLADLCIVPQVFNAQRFSIGLERFPTIRRIHDHAMTLDAFKAAAPAAQPDAE

Specific function: Forms An Equimolar Complex With The RNA Polymerase Holoenzyme (Rnap) But Not With The Core Enzyme. It Is Synthesized Predominantly When Cells Are Exposed To Amino Acid Starvation, At Which Time It Accounts For Over 50% Of The Total Protein Synthesized. It

COG id: COG0625

COG function: function code O; Glutathione S-transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GST N-terminal domain [H]

Homologues:

Organism=Homo sapiens, GI22202624, Length=211, Percent_Identity=46.9194312796209, Blast_Score=187, Evalue=8e-48,
Organism=Homo sapiens, GI194440732, Length=212, Percent_Identity=34.9056603773585, Blast_Score=129, Evalue=2e-30,
Organism=Homo sapiens, GI22202622, Length=160, Percent_Identity=43.75, Blast_Score=124, Evalue=9e-29,
Organism=Caenorhabditis elegans, GI17551302, Length=211, Percent_Identity=41.7061611374408, Blast_Score=154, Evalue=4e-38,
Organism=Caenorhabditis elegans, GI17510461, Length=211, Percent_Identity=40.2843601895735, Blast_Score=149, Evalue=8e-37,
Organism=Caenorhabditis elegans, GI17556142, Length=212, Percent_Identity=38.2075471698113, Blast_Score=133, Evalue=7e-32,
Organism=Drosophila melanogaster, GI21355859, Length=212, Percent_Identity=45.2830188679245, Blast_Score=181, Evalue=3e-46,
Organism=Drosophila melanogaster, GI24645375, Length=212, Percent_Identity=45.2830188679245, Blast_Score=181, Evalue=3e-46,
Organism=Drosophila melanogaster, GI45553325, Length=212, Percent_Identity=45.2830188679245, Blast_Score=181, Evalue=4e-46,
Organism=Drosophila melanogaster, GI21355857, Length=213, Percent_Identity=43.6619718309859, Blast_Score=167, Evalue=4e-42,

Paralogues:

None

Copy number: 480 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1982 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010987
- InterPro:   IPR004045
- InterPro:   IPR017933
- InterPro:   IPR004046
- InterPro:   IPR005955
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF00043 GST_C; PF02798 GST_N [H]

EC number: =5.2.1.2 [H]

Molecular weight: Translated: 24078; Mature: 24078

Theoretical pI: Translated: 6.88; Mature: 6.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLYSYFRSSAAFRVRIALHLKKLPFDYVPVHLLRDGGQQLTDDYRALNPDALVPTLVDG
CCHHHHHHCCCCEEEEEEEEHHHCCCCHHHHHHHHCCCHHHHHHHHHCCCCCCCCEEECC
DAPLQQSLAIVEYLDEAYPAVPLLPQAPVDRAYVRAIALQVACEIHPLNNLRVLKYLKHT
CCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHEECCCCCHHHHHHHHHH
LKVDDDAKDAWYRHWIEDGFKSLEARLSGDPRTGKLCFGDTPTLADLCIVPQVFNAQRFS
HCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCHHHHHHHHHHHCCHHHH
IGLERFPTIRRIHDHAMTLDAFKAAAPAAQPDAE
HCHHHCHHHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MKLYSYFRSSAAFRVRIALHLKKLPFDYVPVHLLRDGGQQLTDDYRALNPDALVPTLVDG
CCHHHHHHCCCCEEEEEEEEHHHCCCCHHHHHHHHCCCHHHHHHHHHCCCCCCCCEEECC
DAPLQQSLAIVEYLDEAYPAVPLLPQAPVDRAYVRAIALQVACEIHPLNNLRVLKYLKHT
CCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHEECCCCCHHHHHHHHHH
LKVDDDAKDAWYRHWIEDGFKSLEARLSGDPRTGKLCFGDTPTLADLCIVPQVFNAQRFS
HCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCHHHHHHHHHHHCCHHHH
IGLERFPTIRRIHDHAMTLDAFKAAAPAAQPDAE
HCHHHCHHHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]