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The map label for this gene is citA [H]

Identifier: 126454714

GI number: 126454714

Start: 236562

End: 237854

Strand: Direct

Name: citA [H]

Synonym: BURPS1106A_0253

Alternate gene names: 126454714

Gene position: 236562-237854 (Clockwise)

Preceding gene: 126452268

Following gene: 126452735

Centisome position: 5.93

GC content: 67.67

Gene sequence:

>1293_bases
ATGGCATCCATTGCGGCTTCGACGAAAACGGCGGGGCAGGTGTTGAACCGGCGCGCGGTGGTGGCGGCCGTGATCGGCAA
CGCGCTCGAATGGTACGACTTCACCGTCTTCAGCTTCATGACGGTCGTGATCGCCCAGTTGTTCTTTCCGACCAGCAGCG
AATATTCGTCGCTGCTCCTCACCACCGCGACGTTCGGCGTCGCGTTCTTCATGCGGCCGATCGGCGGCATCGTGCTTGGC
CTCTACGCGGATCGCGCGGGGCGCAAGGCGGCGCTGTCGCTCGTGATCCTGCTGATGACGTTCGGCATCTTCCTGATCGC
GGTCGCGCCGCCCTATGCGGCGATCGGCATCGGCGGGCCGCTTCTCATCGTGCTCGGCCGCCTGCTGCAGGGCTTCTCCG
CAGGCGGCGAATTCGGCAGCGCGACCGCGCTGCTGATCGAGGCGGCGCCGCTGTCGCGGCGCGGCTACTACGGCAGCTGG
CAGATGGCGAGCCAGGCGGCGGCGCTGCTGTTCGGCTCGCTCGTCGGGGCGGCCGTCACGCGCGGCCTGTCGACCGAGGC
GCTGCACGCGTGGGGCTGGCGCGTGCCGTTCCTGATCGGGCTCGTGATCGGGCCCGTCGGTTTCTACGTGCGCCGCCATC
TCGTCGATTCGGAAGCGTTCCTGCAGGCGCGCGAGCGCGGCAAGCACGTGACGCTCGGTGAAGTGTTCGCGCGGCACGGG
CGTGCGGTGGTGTGCGGGCTCGGCGCGGTGATCGCGCTCACCGTCACCGTCTACGTGCTGATCAGCTATCTGCCGACGTT
CGCCGTCAAGCAACTGAAGCTGCCTTACGCGCAATCGTTCTATGCGGTGATCATCGGCAACCTGTTGCTGACCGCGCTGT
CGCCCGTCGCGGGCGCGTGGTCCGACCGCATCGGGCGCAAGGGGTTGTCGCTGTGGGCGCTCGGGCTCACGCTCGTGATG
ATCTATCCGCTCTTCGCCTGGCTCGACGCGTCGCCGAGCATCGCGCGGCTGATCGCGGTGCAGGCGATGCTCTCGGTGAC
GCTCGCCGGCTACTATGGGCCGTTCGGCGCGATGATCGCCGAGCTTTTTCCGGCGAACGTGCGCTCGACGGGGCTGTCGC
TCGCGTATAACGTCGCGGTGATGGTGTTCGGCGGCTTCGGCCAGTTCATCGTCACGTGGCTCATCAAGACGACGGGCACG
CCGCTTGCGCCGACTTATTACGTGATGGCCGGGCTCGCGCTGTCGCTCGTCGCGCTCGCGTTCGTGCCGACAGAGCGCGA
TGCGCTCGATTGA

Upstream 100 bases:

>100_bases
CGCCCGGCGCGGCGTTCGCTTTTTTTTCGGCGACGCTATTCACGCACGGTGCGCGCCATGCGGCGACATCACGAAAGCAC
GACGACAGCGAGGGGCGAGG

Downstream 100 bases:

>100_bases
CCGATCTCGGCCGCAGGCCTCGCCGAGGGGCCGCACCGCCTCGCCGTGCGCGTGGCTCGCCGTCCGAGCGTCTCGCTGCC
GATGTCCGATGCCGGACGCC

Product: major facilitator family transporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Citrate carrier protein; Citrate transporter; Citrate utilization determinant; Citrate utilization protein A [H]

Number of amino acids: Translated: 430; Mature: 429

Protein sequence:

>430_residues
MASIAASTKTAGQVLNRRAVVAAVIGNALEWYDFTVFSFMTVVIAQLFFPTSSEYSSLLLTTATFGVAFFMRPIGGIVLG
LYADRAGRKAALSLVILLMTFGIFLIAVAPPYAAIGIGGPLLIVLGRLLQGFSAGGEFGSATALLIEAAPLSRRGYYGSW
QMASQAAALLFGSLVGAAVTRGLSTEALHAWGWRVPFLIGLVIGPVGFYVRRHLVDSEAFLQARERGKHVTLGEVFARHG
RAVVCGLGAVIALTVTVYVLISYLPTFAVKQLKLPYAQSFYAVIIGNLLLTALSPVAGAWSDRIGRKGLSLWALGLTLVM
IYPLFAWLDASPSIARLIAVQAMLSVTLAGYYGPFGAMIAELFPANVRSTGLSLAYNVAVMVFGGFGQFIVTWLIKTTGT
PLAPTYYVMAGLALSLVALAFVPTERDALD

Sequences:

>Translated_430_residues
MASIAASTKTAGQVLNRRAVVAAVIGNALEWYDFTVFSFMTVVIAQLFFPTSSEYSSLLLTTATFGVAFFMRPIGGIVLG
LYADRAGRKAALSLVILLMTFGIFLIAVAPPYAAIGIGGPLLIVLGRLLQGFSAGGEFGSATALLIEAAPLSRRGYYGSW
QMASQAAALLFGSLVGAAVTRGLSTEALHAWGWRVPFLIGLVIGPVGFYVRRHLVDSEAFLQARERGKHVTLGEVFARHG
RAVVCGLGAVIALTVTVYVLISYLPTFAVKQLKLPYAQSFYAVIIGNLLLTALSPVAGAWSDRIGRKGLSLWALGLTLVM
IYPLFAWLDASPSIARLIAVQAMLSVTLAGYYGPFGAMIAELFPANVRSTGLSLAYNVAVMVFGGFGQFIVTWLIKTTGT
PLAPTYYVMAGLALSLVALAFVPTERDALD
>Mature_429_residues
ASIAASTKTAGQVLNRRAVVAAVIGNALEWYDFTVFSFMTVVIAQLFFPTSSEYSSLLLTTATFGVAFFMRPIGGIVLGL
YADRAGRKAALSLVILLMTFGIFLIAVAPPYAAIGIGGPLLIVLGRLLQGFSAGGEFGSATALLIEAAPLSRRGYYGSWQ
MASQAAALLFGSLVGAAVTRGLSTEALHAWGWRVPFLIGLVIGPVGFYVRRHLVDSEAFLQARERGKHVTLGEVFARHGR
AVVCGLGAVIALTVTVYVLISYLPTFAVKQLKLPYAQSFYAVIIGNLLLTALSPVAGAWSDRIGRKGLSLWALGLTLVMI
YPLFAWLDASPSIARLIAVQAMLSVTLAGYYGPFGAMIAELFPANVRSTGLSLAYNVAVMVFGGFGQFIVTWLIKTTGTP
LAPTYYVMAGLALSLVALAFVPTERDALD

Specific function: Uptake of citrate across the boundary membrane with the concomitant transport of protons into the cell (symport system) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=406, Percent_Identity=31.5270935960591, Blast_Score=230, Evalue=1e-61,
Organism=Escherichia coli, GI1788942, Length=412, Percent_Identity=33.7378640776699, Blast_Score=192, Evalue=4e-50,
Organism=Escherichia coli, GI1789941, Length=421, Percent_Identity=32.3040380047506, Blast_Score=173, Evalue=2e-44,
Organism=Escherichia coli, GI1788292, Length=421, Percent_Identity=29.4536817102138, Blast_Score=164, Evalue=1e-41,
Organism=Escherichia coli, GI87082231, Length=217, Percent_Identity=28.110599078341, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI87082404, Length=373, Percent_Identity=24.9329758713137, Blast_Score=69, Evalue=8e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 45640; Mature: 45508

Theoretical pI: Translated: 10.04; Mature: 10.04

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1 ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASIAASTKTAGQVLNRRAVVAAVIGNALEWYDFTVFSFMTVVIAQLFFPTSSEYSSLLL
CCCCCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
TTATFGVAFFMRPIGGIVLGLYADRAGRKAALSLVILLMTFGIFLIAVAPPYAAIGIGGP
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCHH
LLIVLGRLLQGFSAGGEFGSATALLIEAAPLSRRGYYGSWQMASQAAALLFGSLVGAAVT
HHHHHHHHHHHHCCCCCCCCHHEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
RGLSTEALHAWGWRVPFLIGLVIGPVGFYVRRHLVDSEAFLQARERGKHVTLGEVFARHG
CCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCHHHHHHHCC
RAVVCGLGAVIALTVTVYVLISYLPTFAVKQLKLPYAQSFYAVIIGNLLLTALSPVAGAW
CEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
SDRIGRKGLSLWALGLTLVMIYPLFAWLDASPSIARLIAVQAMLSVTLAGYYGPFGAMIA
HHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHH
ELFPANVRSTGLSLAYNVAVMVFGGFGQFIVTWLIKTTGTPLAPTYYVMAGLALSLVALA
HHCCCCHHHHCHHHHHHHHHHEECCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHH
FVPTERDALD
HCCCCCCCCC
>Mature Secondary Structure 
ASIAASTKTAGQVLNRRAVVAAVIGNALEWYDFTVFSFMTVVIAQLFFPTSSEYSSLLL
CCCCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
TTATFGVAFFMRPIGGIVLGLYADRAGRKAALSLVILLMTFGIFLIAVAPPYAAIGIGGP
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCHH
LLIVLGRLLQGFSAGGEFGSATALLIEAAPLSRRGYYGSWQMASQAAALLFGSLVGAAVT
HHHHHHHHHHHHCCCCCCCCHHEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
RGLSTEALHAWGWRVPFLIGLVIGPVGFYVRRHLVDSEAFLQARERGKHVTLGEVFARHG
CCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCHHHHHHHCC
RAVVCGLGAVIALTVTVYVLISYLPTFAVKQLKLPYAQSFYAVIIGNLLLTALSPVAGAW
CEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
SDRIGRKGLSLWALGLTLVMIYPLFAWLDASPSIARLIAVQAMLSVTLAGYYGPFGAMIA
HHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHH
ELFPANVRSTGLSLAYNVAVMVFGGFGQFIVTWLIKTTGTPLAPTYYVMAGLALSLVALA
HHCCCCHHHHCHHHHHHHHHHEECCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHH
FVPTERDALD
HCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]