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The map label for this gene is narK [H]

Identifier: 126454415

GI number: 126454415

Start: 2641681

End: 2643153

Strand: Direct

Name: narK [H]

Synonym: BURPS1106A_2679

Alternate gene names: 126454415

Gene position: 2641681-2643153 (Clockwise)

Preceding gene: 126451711

Following gene: 126451639

Centisome position: 66.23

GC content: 67.89

Gene sequence:

>1473_bases
GTGCCGCGCACGACCAGCGCGTGCTCGCCACCCGCTCCGCGTGAGCCTTGCTTCCTCGTCATCTTCGTTCACTCAACGGA
TTCGATCATGTCCACTTCTCTACTCGTGCGCTGGGATCCCGAAAACCCGGCCTTCTGGCAAGCCAAGGGGCGCCCCGTCG
CGTGGCGCAATCTCGCGATCTCGATTCCCGCGCTGATGCTCGCGTTCGTCGTCTGGTCGCTCTGGAGCGTCGTCGTCGTC
AACCTCGATCGCGCGGGCTTTCACTTCAGCAAGAACCAGCTGTTCTGGCTCACCGCGCTGCCCGCGCTCTCCGGCGCGAC
GCTGCGCATCTTCTATTCGTTCCTCGTGCCGATCTTCGGCGGCCGCCGCTTCACCGCGATCTCGACCGCGACGCTGCTGA
TTCCCGCGCTCGGAATGGGCTTCGCGCTGCGCGACCCCGGCACGGGCTACCCGACGCTCCTCATCCTCGCGCTGCTCTGC
GGGTTCGGCGGCGCGAACTTCAGCTCGTCGATGGCGAACATCAGCTTCTTCTTCCCGAAGGCGAAGAAAGGACTCGCGAC
CGGCCTGAACGCGGGCATCGGCAACCTCGGCGTGTCGGTCGTGCAGTTCGTCACGCCGCTCGTGATCTCGGCGGGCCTGT
TCGGCGCGCTCGCGGGCGATCCGCAGAGCGTCGTCGCGCACGGCGCGACGACGAACCTGTGGTTGCAGAACGCAGGCTTC
GTGTGGGTGCCGTTCATCGTCGTCGCGACGCTCGCCGCGTGGTTCGGGATGAATGACATCGCCGACGCGAAGGCGTCGTT
CGCCGAGCAGGCGGTGATCTTCCGCCGGCTGCACAACTGGCTGATGTGCTGGCTGTACGTCGGCACCTTCGGTTCGTTCA
TCGGCTTCTCGGCGGGCTTCGCGCTCCTCACGAAGGCGCTCTTCCCGAACGTGAACCCGACCGCGTACGCGTTCATCGGC
CCGCTCGCGGGCGCGCTGATGCGCCCCGTCGGCGGATGGGTGTCCGACCGCATCGGCGGCGCGCGCGTGACGTTCTGGAC
CTTCGCCGCGATGATCGCGGCCGTCGGCGGCGTGATCGCGACGCTGCCCTCGGGCGGCGCCGCGGGCAGCTTCGCGGGCT
TCCTCGCGATGTTCATCGTGCTGTTCGCGCTCACGGGCATCGGCAACGGCTCGACGTTCCGGATGATCCCGGTGATCTTC
CTCACCGAGCGGCAGCGCGCCGCGCAAGGCCAGGACGAGGCCGCGCGCAAGCAGGCGGTGCTCGACGCCGGCAAGGAATC
GGCCGCGGTGCTCGGCTTTTCGGGCGCGATCGGCGCGTACGGCGGCTTCTTCATCCCGAAGAGTTTCGGCACGTCGCTCG
ACATGACGGGCTCGGCCGTGCCGGCGCTCGTGTGCTTCATCGCGTTCTACGTGTCGTGCGTCGCGATCACCTGGTGGTTC
TACGCGCGCCGCAACGCGTCGATGCCGTGCTGA

Upstream 100 bases:

>100_bases
CTCGTCGACCTCACGGGCGTGCGCTCGACCTGCTTCATGCTGCTCTACGGCGCCGTCAGCGTGAGCCTCGTCTGGATGTA
CTTCAGCTTCCGCGCCGAGC

Downstream 100 bases:

>100_bases
CGATAGCGCGCTGACGCCTCCCGCCTCTTTGCCGGATGCGGCGCGCTGCGACCAGCGGCCGCACCCGGTTCGCATTGCCC
GAGGGCGCGCCGCGCCCTCT

Product: nitrite extrusion protein

Products: NA

Alternate protein names: Nitrite facilitator 1 [H]

Number of amino acids: Translated: 490; Mature: 489

Protein sequence:

>490_residues
MPRTTSACSPPAPREPCFLVIFVHSTDSIMSTSLLVRWDPENPAFWQAKGRPVAWRNLAISIPALMLAFVVWSLWSVVVV
NLDRAGFHFSKNQLFWLTALPALSGATLRIFYSFLVPIFGGRRFTAISTATLLIPALGMGFALRDPGTGYPTLLILALLC
GFGGANFSSSMANISFFFPKAKKGLATGLNAGIGNLGVSVVQFVTPLVISAGLFGALAGDPQSVVAHGATTNLWLQNAGF
VWVPFIVVATLAAWFGMNDIADAKASFAEQAVIFRRLHNWLMCWLYVGTFGSFIGFSAGFALLTKALFPNVNPTAYAFIG
PLAGALMRPVGGWVSDRIGGARVTFWTFAAMIAAVGGVIATLPSGGAAGSFAGFLAMFIVLFALTGIGNGSTFRMIPVIF
LTERQRAAQGQDEAARKQAVLDAGKESAAVLGFSGAIGAYGGFFIPKSFGTSLDMTGSAVPALVCFIAFYVSCVAITWWF
YARRNASMPC

Sequences:

>Translated_490_residues
MPRTTSACSPPAPREPCFLVIFVHSTDSIMSTSLLVRWDPENPAFWQAKGRPVAWRNLAISIPALMLAFVVWSLWSVVVV
NLDRAGFHFSKNQLFWLTALPALSGATLRIFYSFLVPIFGGRRFTAISTATLLIPALGMGFALRDPGTGYPTLLILALLC
GFGGANFSSSMANISFFFPKAKKGLATGLNAGIGNLGVSVVQFVTPLVISAGLFGALAGDPQSVVAHGATTNLWLQNAGF
VWVPFIVVATLAAWFGMNDIADAKASFAEQAVIFRRLHNWLMCWLYVGTFGSFIGFSAGFALLTKALFPNVNPTAYAFIG
PLAGALMRPVGGWVSDRIGGARVTFWTFAAMIAAVGGVIATLPSGGAAGSFAGFLAMFIVLFALTGIGNGSTFRMIPVIF
LTERQRAAQGQDEAARKQAVLDAGKESAAVLGFSGAIGAYGGFFIPKSFGTSLDMTGSAVPALVCFIAFYVSCVAITWWF
YARRNASMPC
>Mature_489_residues
PRTTSACSPPAPREPCFLVIFVHSTDSIMSTSLLVRWDPENPAFWQAKGRPVAWRNLAISIPALMLAFVVWSLWSVVVVN
LDRAGFHFSKNQLFWLTALPALSGATLRIFYSFLVPIFGGRRFTAISTATLLIPALGMGFALRDPGTGYPTLLILALLCG
FGGANFSSSMANISFFFPKAKKGLATGLNAGIGNLGVSVVQFVTPLVISAGLFGALAGDPQSVVAHGATTNLWLQNAGFV
WVPFIVVATLAAWFGMNDIADAKASFAEQAVIFRRLHNWLMCWLYVGTFGSFIGFSAGFALLTKALFPNVNPTAYAFIGP
LAGALMRPVGGWVSDRIGGARVTFWTFAAMIAAVGGVIATLPSGGAAGSFAGFLAMFIVLFALTGIGNGSTFRMIPVIFL
TERQRAAQGQDEAARKQAVLDAGKESAAVLGFSGAIGAYGGFFIPKSFGTSLDMTGSAVPALVCFIAFYVSCVAITWWFY
ARRNASMPC

Specific function: Involved in excretion of nitrite produced by the dissimilatory reduction of nitrate [H]

COG id: COG2223

COG function: function code P; Nitrate/nitrite transporter

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Nitrate/nitrite porter (TC 2.A.1.8) family [H]

Homologues:

Organism=Escherichia coli, GI1787475, Length=466, Percent_Identity=53.6480686695279, Blast_Score=450, Evalue=1e-128,
Organism=Escherichia coli, GI1787743, Length=462, Percent_Identity=54.1125541125541, Blast_Score=417, Evalue=1e-117,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR004737 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 52041; Mature: 51910

Theoretical pI: Translated: 10.22; Mature: 10.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRTTSACSPPAPREPCFLVIFVHSTDSIMSTSLLVRWDPENPAFWQAKGRPVAWRNLAI
CCCCCCCCCCCCCCCCEEEEEEEECCCCHHEEEEEEEECCCCCCEEECCCCCEEHHHHHH
SIPALMLAFVVWSLWSVVVVNLDRAGFHFSKNQLFWLTALPALSGATLRIFYSFLVPIFG
HHHHHHHHHHHHHHHHHHHHCCCCCCCEECCCCEEEEEECCCCCCHHHHHHHHHHHHHHC
GRRFTAISTATLLIPALGMGFALRDPGTGYPTLLILALLCGFGGANFSSSMANISFFFPK
CCEEEHHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHCCCCCCCCCCCCCEEEECCH
AKKGLATGLNAGIGNLGVSVVQFVTPLVISAGLFGALAGDPQSVVAHGATTNLWLQNAGF
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCEEEEECCCC
VWVPFIVVATLAAWFGMNDIADAKASFAEQAVIFRRLHNWLMCWLYVGTFGSFIGFSAGF
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALLTKALFPNVNPTAYAFIGPLAGALMRPVGGWVSDRIGGARVTFWTFAAMIAAVGGVIA
HHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCHHHHCCCCCCHHHHHHHHHHHHHHHHHH
TLPSGGAAGSFAGFLAMFIVLFALTGIGNGSTFRMIPVIFLTERQRAAQGQDEAARKQAV
CCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEEECHHHHHCCCHHHHHHHHH
LDAGKESAAVLGFSGAIGAYGGFFIPKSFGTSLDMTGSAVPALVCFIAFYVSCVAITWWF
HHCCCCCCEEEEECCCHHCCCCEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
YARRNASMPC
HHHCCCCCCC
>Mature Secondary Structure 
PRTTSACSPPAPREPCFLVIFVHSTDSIMSTSLLVRWDPENPAFWQAKGRPVAWRNLAI
CCCCCCCCCCCCCCCEEEEEEEECCCCHHEEEEEEEECCCCCCEEECCCCCEEHHHHHH
SIPALMLAFVVWSLWSVVVVNLDRAGFHFSKNQLFWLTALPALSGATLRIFYSFLVPIFG
HHHHHHHHHHHHHHHHHHHHCCCCCCCEECCCCEEEEEECCCCCCHHHHHHHHHHHHHHC
GRRFTAISTATLLIPALGMGFALRDPGTGYPTLLILALLCGFGGANFSSSMANISFFFPK
CCEEEHHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHCCCCCCCCCCCCCEEEECCH
AKKGLATGLNAGIGNLGVSVVQFVTPLVISAGLFGALAGDPQSVVAHGATTNLWLQNAGF
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCEEEEECCCC
VWVPFIVVATLAAWFGMNDIADAKASFAEQAVIFRRLHNWLMCWLYVGTFGSFIGFSAGF
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALLTKALFPNVNPTAYAFIGPLAGALMRPVGGWVSDRIGGARVTFWTFAAMIAAVGGVIA
HHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCHHHHCCCCCCHHHHHHHHHHHHHHHHHH
TLPSGGAAGSFAGFLAMFIVLFALTGIGNGSTFRMIPVIFLTERQRAAQGQDEAARKQAV
CCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEEECHHHHHCCCHHHHHHHHH
LDAGKESAAVLGFSGAIGAYGGFFIPKSFGTSLDMTGSAVPALVCFIAFYVSCVAITWWF
HHCCCCCCEEEEECCCHHCCCCEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
YARRNASMPC
HHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2668029; 8905232; 9278503; 2657652 [H]