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The map label for this gene is gatA [H]

Identifier: 126454395

GI number: 126454395

Start: 2916345

End: 2918195

Strand: Direct

Name: gatA [H]

Synonym: BURPS1106A_2972

Alternate gene names: 126454395

Gene position: 2916345-2918195 (Clockwise)

Preceding gene: 126451975

Following gene: 126455293

Centisome position: 73.12

GC content: 74.28

Gene sequence:

>1851_bases
GTGGGCCACCGTGACGCGACGCCGCGCACCATTCGGCATGCGAAGCGAGGCGCGCGGCGCGCAACAGCAGCGCTCAGCGG
CGGCGCGACAGCGCGCGCCGCGCGGACGGCTTCGACGCCGCGACACCTCGACGCCGAGCCCGCGCGCGATTCCGTATCGA
TGCGCGAGGCGCGCGAACGGACGCGCGCGAAACGACAGCCGCATGCGATATCGCACGCGAACCGCGGTCAAACGCGTTCC
CGTCGCGATCGACCGCCCCCCGCGCCCCTTCGTTGCCCGCCCCGACGTCCGCTCGCGCGGCCGTGCGCCGTCGGCGCCGC
GTCGCATGCGAAGCGCCCGCGCGCGCCGCCGCGCCTCCGCGGCGCAACGCTGTTCTACAATGCGCGAACCGGCCGCCGAG
CGCCATCGCGCGGCTTCGCGAAGCGCCGAATCGTGCCGATCCGCTTCGTCAACCCGATCGAACCCGATACCGTCATGACC
ACTTTCGCCCCCTTCCCGCCGCTCGCCCGCCTCGCCGCCGATCTCGCCGCCGGCGTGACGACGAGCCGCGCGCTCGTCGA
AGCCGCGCTCGAACGGATCGCCGATCCGGCGGGGCAAGGCGCGCTCGCGTTCACCGAGGTCGACGCGCCGCGCGCGCGCG
CGGCCGCCGACGCGCACGATCGCCTGCGCGCGGCGGGCACCGTGCTGTCACCGCTCGCGGGCATTCCGGTGTCGGTGAAG
GATCTGTTCGACGTCGCGGGCCAGGTCACGCGCGCGGGCTCCCGCGTGCTCGCCGACGCGCCCCCGGCCGCCGACGACGC
CGTCGCGGTCGCCCGCCTCAAGCGCGCGGGCGCCGTGCTCGTCGGCCGCACGAACATGAGCGAATTCGCGTTCTCCGGCC
TGGGCCTCAATCCGCATTACGGCACGCCGCGCTCGCCGTACCGGCGCGACGTGCCGGGCGACGAGCGGATCGCGGGCGGC
TCGTCGTCGGGCGCGGCCGCATCGGTTGCGGACGGCATGGCGGCCGTCGCGCTCGCGACCGATACGGGCGGCTCGATCCG
CATCCCCGCCGCGCTCTGCGGACTGACGGGCTTCAAGCCGACCGCGAGCCGGATTCCGAAGCAAGGCGGCGTGCCGCTGT
CGACGACGCTCGATTCGTTCGGCCCGATCGGCGTATCGGTCGCGTGCTGCGCGCTCGTCGACCGGATCCTCGCGGGCCTC
GAGCCGCACGTGTGCGCCGCGCGCCCGCTCGAGGGCGTGCGGCTCGGCGTGCTGACGAACTATGTGACGGACGGCATGGA
CGCGCACGTCGCCACGTCGCTCGACGCCGCGCTCAAGCATCTGGAAGCGGCGGGCGCGATCGTGTCGGACGCGCGCTTCG
CGCCGCTCGACACGCTGCCCGGCATCAACCGCATCGGTTTCGCTTCGATCGAGGCGCATGCGTGGCATCGCCCGCTCGTC
GCGCGCCACCGCGACGCGTACGATCCGCGCGTGCTCGCGCGCATCCTGAAGGGCGAGCCGGCGAGCGCCGCCGATTACCT
CGATCTCGTCGCCGCGCGCGCGGCGCTCATCGACGAGGCGGCGCGCACCGTGTGGCAGCGCTTCGATGCGCTCGTCGCGC
CGACGGTGCCCATCGCGCCGCCGCGCATCGCCGAGCTCGAACGGGACGACGACGCGTTCGCCCGCACGAACGCGCTCGTG
CTGCGCAACCCGAGCGCGTTCAATTTTCTCGATGCGTGCGCGCTATCGCTGCCGTGCCATCCGCGCGACACGGCGCCCGT
CGGCCTGATGCTCGCCGCCGCACCGCATCACGACGACGCGCTGCTCGCGATCGGCCAGGCGGTCGAAGCGGTGCTCGCGA
CGATCCGCTGA

Upstream 100 bases:

>100_bases
GAGAATCAACAAGAATTCATTATGCTTCAGTACACGATCGACGGCAAACCGACAGAATTTCGTCAATATTGTTGCAACGT
TGTGGCGAATTCACGCCGAT

Downstream 100 bases:

>100_bases
GCGCCCCCCACCCGCGCGCCGGATGCCGCGCCGTCGAAAAGCGGCGCGACGTTCGCGTTAAAATCCGACGATTCATTTTC
CCCGACCGCTCGAAGGAATC

Product: amidase

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 616; Mature: 615

Protein sequence:

>616_residues
MGHRDATPRTIRHAKRGARRATAALSGGATARAARTASTPRHLDAEPARDSVSMREARERTRAKRQPHAISHANRGQTRS
RRDRPPPAPLRCPPRRPLARPCAVGAASHAKRPRAPPRLRGATLFYNARTGRRAPSRGFAKRRIVPIRFVNPIEPDTVMT
TFAPFPPLARLAADLAAGVTTSRALVEAALERIADPAGQGALAFTEVDAPRARAAADAHDRLRAAGTVLSPLAGIPVSVK
DLFDVAGQVTRAGSRVLADAPPAADDAVAVARLKRAGAVLVGRTNMSEFAFSGLGLNPHYGTPRSPYRRDVPGDERIAGG
SSSGAAASVADGMAAVALATDTGGSIRIPAALCGLTGFKPTASRIPKQGGVPLSTTLDSFGPIGVSVACCALVDRILAGL
EPHVCAARPLEGVRLGVLTNYVTDGMDAHVATSLDAALKHLEAAGAIVSDARFAPLDTLPGINRIGFASIEAHAWHRPLV
ARHRDAYDPRVLARILKGEPASAADYLDLVAARAALIDEAARTVWQRFDALVAPTVPIAPPRIAELERDDDAFARTNALV
LRNPSAFNFLDACALSLPCHPRDTAPVGLMLAAAPHHDDALLAIGQAVEAVLATIR

Sequences:

>Translated_616_residues
MGHRDATPRTIRHAKRGARRATAALSGGATARAARTASTPRHLDAEPARDSVSMREARERTRAKRQPHAISHANRGQTRS
RRDRPPPAPLRCPPRRPLARPCAVGAASHAKRPRAPPRLRGATLFYNARTGRRAPSRGFAKRRIVPIRFVNPIEPDTVMT
TFAPFPPLARLAADLAAGVTTSRALVEAALERIADPAGQGALAFTEVDAPRARAAADAHDRLRAAGTVLSPLAGIPVSVK
DLFDVAGQVTRAGSRVLADAPPAADDAVAVARLKRAGAVLVGRTNMSEFAFSGLGLNPHYGTPRSPYRRDVPGDERIAGG
SSSGAAASVADGMAAVALATDTGGSIRIPAALCGLTGFKPTASRIPKQGGVPLSTTLDSFGPIGVSVACCALVDRILAGL
EPHVCAARPLEGVRLGVLTNYVTDGMDAHVATSLDAALKHLEAAGAIVSDARFAPLDTLPGINRIGFASIEAHAWHRPLV
ARHRDAYDPRVLARILKGEPASAADYLDLVAARAALIDEAARTVWQRFDALVAPTVPIAPPRIAELERDDDAFARTNALV
LRNPSAFNFLDACALSLPCHPRDTAPVGLMLAAAPHHDDALLAIGQAVEAVLATIR
>Mature_615_residues
GHRDATPRTIRHAKRGARRATAALSGGATARAARTASTPRHLDAEPARDSVSMREARERTRAKRQPHAISHANRGQTRSR
RDRPPPAPLRCPPRRPLARPCAVGAASHAKRPRAPPRLRGATLFYNARTGRRAPSRGFAKRRIVPIRFVNPIEPDTVMTT
FAPFPPLARLAADLAAGVTTSRALVEAALERIADPAGQGALAFTEVDAPRARAAADAHDRLRAAGTVLSPLAGIPVSVKD
LFDVAGQVTRAGSRVLADAPPAADDAVAVARLKRAGAVLVGRTNMSEFAFSGLGLNPHYGTPRSPYRRDVPGDERIAGGS
SSGAAASVADGMAAVALATDTGGSIRIPAALCGLTGFKPTASRIPKQGGVPLSTTLDSFGPIGVSVACCALVDRILAGLE
PHVCAARPLEGVRLGVLTNYVTDGMDAHVATSLDAALKHLEAAGAIVSDARFAPLDTLPGINRIGFASIEAHAWHRPLVA
RHRDAYDPRVLARILKGEPASAADYLDLVAARAALIDEAARTVWQRFDALVAPTVPIAPPRIAELERDDDAFARTNALVL
RNPSAFNFLDACALSLPCHPRDTAPVGLMLAAAPHHDDALLAIGQAVEAVLATIR

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=250, Percent_Identity=33.2, Blast_Score=92, Evalue=2e-18,
Organism=Homo sapiens, GI195972892, Length=197, Percent_Identity=32.48730964467, Blast_Score=91, Evalue=3e-18,
Organism=Homo sapiens, GI166795287, Length=138, Percent_Identity=39.1304347826087, Blast_Score=76, Evalue=9e-14,
Organism=Caenorhabditis elegans, GI17543272, Length=437, Percent_Identity=24.9427917620137, Blast_Score=107, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI71990152, Length=170, Percent_Identity=37.0588235294118, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17538252, Length=159, Percent_Identity=33.3333333333333, Blast_Score=87, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17537465, Length=360, Percent_Identity=26.3888888888889, Blast_Score=83, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17556264, Length=135, Percent_Identity=38.5185185185185, Blast_Score=81, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6319685, Length=390, Percent_Identity=28.974358974359, Blast_Score=104, Evalue=5e-23,
Organism=Saccharomyces cerevisiae, GI6323950, Length=153, Percent_Identity=38.562091503268, Blast_Score=72, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24648113, Length=280, Percent_Identity=33.5714285714286, Blast_Score=111, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24648435, Length=216, Percent_Identity=33.7962962962963, Blast_Score=87, Evalue=4e-17,
Organism=Drosophila melanogaster, GI24648437, Length=216, Percent_Identity=33.7962962962963, Blast_Score=87, Evalue=4e-17,
Organism=Drosophila melanogaster, GI45550774, Length=216, Percent_Identity=33.7962962962963, Blast_Score=87, Evalue=4e-17,
Organism=Drosophila melanogaster, GI24648441, Length=146, Percent_Identity=37.6712328767123, Blast_Score=85, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24648439, Length=146, Percent_Identity=37.6712328767123, Blast_Score=85, Evalue=1e-16,
Organism=Drosophila melanogaster, GI21356731, Length=142, Percent_Identity=37.3239436619718, Blast_Score=73, Evalue=5e-13,
Organism=Drosophila melanogaster, GI24652985, Length=156, Percent_Identity=33.974358974359, Blast_Score=69, Evalue=1e-11,
Organism=Drosophila melanogaster, GI19922090, Length=156, Percent_Identity=33.974358974359, Blast_Score=69, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24652981, Length=156, Percent_Identity=33.974358974359, Blast_Score=69, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24652983, Length=156, Percent_Identity=33.974358974359, Blast_Score=69, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: NA

Molecular weight: Translated: 64696; Mature: 64564

Theoretical pI: Translated: 11.19; Mature: 11.19

Prosite motif: PS00571 AMIDASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGHRDATPRTIRHAKRGARRATAALSGGATARAARTASTPRHLDAEPARDSVSMREARER
CCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHH
TRAKRQPHAISHANRGQTRSRRDRPPPAPLRCPPRRPLARPCAVGAASHAKRPRAPPRLR
HHHHCCCCHHHCCCCCCCHHHCCCCCCCCCCCCCCCCCCCHHHCCCHHHCCCCCCCCCCC
GATLFYNARTGRRAPSRGFAKRRIVPIRFVNPIEPDTVMTTFAPFPPLARLAADLAAGVT
CCEEEEECCCCCCCCCCCHHHCCEEEEEECCCCCCCCEEEECCCCCHHHHHHHHHHHCCH
TSRALVEAALERIADPAGQGALAFTEVDAPRARAAADAHDRLRAAGTVLSPLAGIPVSVK
HHHHHHHHHHHHHCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHH
DLFDVAGQVTRAGSRVLADAPPAADDAVAVARLKRAGAVLVGRTNMSEFAFSGLGLNPHY
HHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHCCEEEEECCCHHHHHHHCCCCCCCC
GTPRSPYRRDVPGDERIAGGSSSGAAASVADGMAAVALATDTGGSIRIPAALCGLTGFKP
CCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCEEEEEEECCCCCEECHHHHHCCCCCCC
TASRIPKQGGVPLSTTLDSFGPIGVSVACCALVDRILAGLEPHVCAARPLEGVRLGVLTN
HHHHCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEHHHHHH
YVTDGMDAHVATSLDAALKHLEAAGAIVSDARFAPLDTLPGINRIGFASIEAHAWHRPLV
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEHHHHHHCCHH
ARHRDAYDPRVLARILKGEPASAADYLDLVAARAALIDEAARTVWQRFDALVAPTVPIAP
HHCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
PRIAELERDDDAFARTNALVLRNPSAFNFLDACALSLPCHPRDTAPVGLMLAAAPHHDDA
CHHHHCCCCCHHHHHCCEEEEECCCHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCH
LLAIGQAVEAVLATIR
HHHHHHHHHHHHHHCC
>Mature Secondary Structure 
GHRDATPRTIRHAKRGARRATAALSGGATARAARTASTPRHLDAEPARDSVSMREARER
CCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHH
TRAKRQPHAISHANRGQTRSRRDRPPPAPLRCPPRRPLARPCAVGAASHAKRPRAPPRLR
HHHHCCCCHHHCCCCCCCHHHCCCCCCCCCCCCCCCCCCCHHHCCCHHHCCCCCCCCCCC
GATLFYNARTGRRAPSRGFAKRRIVPIRFVNPIEPDTVMTTFAPFPPLARLAADLAAGVT
CCEEEEECCCCCCCCCCCHHHCCEEEEEECCCCCCCCEEEECCCCCHHHHHHHHHHHCCH
TSRALVEAALERIADPAGQGALAFTEVDAPRARAAADAHDRLRAAGTVLSPLAGIPVSVK
HHHHHHHHHHHHHCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHH
DLFDVAGQVTRAGSRVLADAPPAADDAVAVARLKRAGAVLVGRTNMSEFAFSGLGLNPHY
HHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHCCEEEEECCCHHHHHHHCCCCCCCC
GTPRSPYRRDVPGDERIAGGSSSGAAASVADGMAAVALATDTGGSIRIPAALCGLTGFKP
CCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCEEEEEEECCCCCEECHHHHHCCCCCCC
TASRIPKQGGVPLSTTLDSFGPIGVSVACCALVDRILAGLEPHVCAARPLEGVRLGVLTN
HHHHCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEHHHHHH
YVTDGMDAHVATSLDAALKHLEAAGAIVSDARFAPLDTLPGINRIGFASIEAHAWHRPLV
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEHHHHHHCCHH
ARHRDAYDPRVLARILKGEPASAADYLDLVAARAALIDEAARTVWQRFDALVAPTVPIAP
HHCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
PRIAELERDDDAFARTNALVLRNPSAFNFLDACALSLPCHPRDTAPVGLMLAAAPHHDDA
CHHHHCCCCCHHHHHCCEEEEECCCHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCH
LLAIGQAVEAVLATIR
HHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA