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The map label for this gene is murD

Identifier: 126453698

GI number: 126453698

Start: 3456295

End: 3457809

Strand: Reverse

Name: murD

Synonym: BURPS1106A_3552

Alternate gene names: 126453698

Gene position: 3457809-3456295 (Counterclockwise)

Preceding gene: 126454548

Following gene: 126452793

Centisome position: 86.7

GC content: 72.61

Gene sequence:

>1515_bases
ATGTTCGGAGATCGGCAGCGGCCGATGGTGCTCGTGCTGGGGCTCGGCGAATCGGGCCTCGCGATCGCGCGGTGGTGCGC
GAGGCACGGGTGCCGGTTGCGCGTCGCCGATACGCGCGAGACGCCGCCCAACCTGGCCGCGCTGACGGCGGCGGGCGTCG
ATTTCGAATTCGTCGGCGGCGCGTTTTCGCCGGCGCTCGTCGACGGCGGGATCGAACTCGTCGCGCTGAGCCCCGGCCTG
TCGCCGCTCGCGGAGGATCTCGCGCCGCTCGTCGCCGCCGCACGCGAGCGCGGCATTCCCGTGTGGGGCGAGCTCGAATT
CTTCGCGCAGGCGCTCGCGGCGCTCGGCGCGAACGGCTACGCGCCGAAGGTGATCGCGATCACCGGCACGAACGGCAAGA
CGACGACGACGAGCCTCGCGGGCCTGCTGTGCGAGCGCGCGGGCAAGAAGGTCGCGGTCGCGGGCAACATCAGCCCGGCG
ATGCTCGACAAGCTGACCGAAGCGATCGACGCGGCGGCGCTGCCGGACGTGTGGGTGCTCGAGCTGTCGAGCTTTCAGCT
CGACACCGCGCACACGTTCGCGCCCGATGCGGCAACGATCCTCAACATCACGCAGGACCATCTCGACTGGCACGGCGGCT
TCGCCGCGTATGCGGCCGCGAAGGGCCGCGTCTTCGGGCCGCGCACGGTGCGCGTGCTCAATCGCGACGATGCCGAGGTG
ATGCGCTTCGCGCCGCCCGCCGCGGCCGCCGACGCGCCGCGCGCGGTCACGTTCGGCCTGAACGAGCCGGCCGCCGACGG
CGATTACGGCCTGCTGCGCGAAAACGGCATCGCATGGCTCGTCGAGGCGATCGATCGCGACGGGGCCGATGCGCCCGCCG
CGCCGTCACGCCGGCGCAAGCAGGAGGCGGCGAATCCGCCCGACATCGCGCTCAAGCGGCTGATGCCCGCCGACGCGTTG
CGCATCCGCGGGCTGCATAACGCGGCGAACGCGCTCGCCGCCTATGCGCTCGCGCGCGCGATCGGCCTGCCGGCCGCGCC
TCTCCTGCACGGCTTGCGCGAATACCGCGGCGAGCCGCACCGCGTCGAAGTGATCGCGACGCTCGACGGCGTCGACTATG
TCGACGACAGCAAGGGCACGAACGTCGGCGCGACGGTCGCGGCGCTCGACGGCCTCGCGCAGCGTGCGGTGCTGATCGCG
GGCGGCGACGGCAAGGGGCAGGATTTCGAGCCGCTCGCGGCGCCCGTCGCGCGCTGGTGCCGCGCGGTGATGCTGATCGG
CCGCGACGCGCCCGCGTTGCGCGAGGCGCTTGCCGACACGGGCGTGCCGCTTGCCGATCACGCGACGCTCGAAGCCGCGG
TGCGCGCGGCGAGCGCGCTCGCGCAGCCGGGGGACGCGGTGCTGCTGTCGCCCGCGTGCGCGAGCCTCGACATGTTCCGC
AATTACGCACACCGGGCCGACGTGTTCCGCAGCGCGGTCGAAGATATCGCCTTGGAAAAAGGAACGACGCTATGA

Upstream 100 bases:

>100_bases
CAGGTGGTGGTGCGTTTCTGGATCATCACGTTGATGCTGTGCCTGTTCGGTTTGTCCACCCTCAAGCTGCGGTAAAGGGA
AGGTAAGGATGTTTGGCGAG

Downstream 100 bases:

>100_bases
GCTGGTCCGATCGCCTCGTTTCCCGTTTCAACGGCGTCCGCGACGCGGGCGGCGCCGCCGCGCCGCGCACCGCCGCACGG
GCGGCCTCGGGCGCGCGCGC

Product: UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Products: NA

Alternate protein names: D-glutamic acid-adding enzyme; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Number of amino acids: Translated: 504; Mature: 504

Protein sequence:

>504_residues
MFGDRQRPMVLVLGLGESGLAIARWCARHGCRLRVADTRETPPNLAALTAAGVDFEFVGGAFSPALVDGGIELVALSPGL
SPLAEDLAPLVAAARERGIPVWGELEFFAQALAALGANGYAPKVIAITGTNGKTTTTSLAGLLCERAGKKVAVAGNISPA
MLDKLTEAIDAAALPDVWVLELSSFQLDTAHTFAPDAATILNITQDHLDWHGGFAAYAAAKGRVFGPRTVRVLNRDDAEV
MRFAPPAAAADAPRAVTFGLNEPAADGDYGLLRENGIAWLVEAIDRDGADAPAAPSRRRKQEAANPPDIALKRLMPADAL
RIRGLHNAANALAAYALARAIGLPAAPLLHGLREYRGEPHRVEVIATLDGVDYVDDSKGTNVGATVAALDGLAQRAVLIA
GGDGKGQDFEPLAAPVARWCRAVMLIGRDAPALREALADTGVPLADHATLEAAVRAASALAQPGDAVLLSPACASLDMFR
NYAHRADVFRSAVEDIALEKGTTL

Sequences:

>Translated_504_residues
MFGDRQRPMVLVLGLGESGLAIARWCARHGCRLRVADTRETPPNLAALTAAGVDFEFVGGAFSPALVDGGIELVALSPGL
SPLAEDLAPLVAAARERGIPVWGELEFFAQALAALGANGYAPKVIAITGTNGKTTTTSLAGLLCERAGKKVAVAGNISPA
MLDKLTEAIDAAALPDVWVLELSSFQLDTAHTFAPDAATILNITQDHLDWHGGFAAYAAAKGRVFGPRTVRVLNRDDAEV
MRFAPPAAAADAPRAVTFGLNEPAADGDYGLLRENGIAWLVEAIDRDGADAPAAPSRRRKQEAANPPDIALKRLMPADAL
RIRGLHNAANALAAYALARAIGLPAAPLLHGLREYRGEPHRVEVIATLDGVDYVDDSKGTNVGATVAALDGLAQRAVLIA
GGDGKGQDFEPLAAPVARWCRAVMLIGRDAPALREALADTGVPLADHATLEAAVRAASALAQPGDAVLLSPACASLDMFR
NYAHRADVFRSAVEDIALEKGTTL
>Mature_504_residues
MFGDRQRPMVLVLGLGESGLAIARWCARHGCRLRVADTRETPPNLAALTAAGVDFEFVGGAFSPALVDGGIELVALSPGL
SPLAEDLAPLVAAARERGIPVWGELEFFAQALAALGANGYAPKVIAITGTNGKTTTTSLAGLLCERAGKKVAVAGNISPA
MLDKLTEAIDAAALPDVWVLELSSFQLDTAHTFAPDAATILNITQDHLDWHGGFAAYAAAKGRVFGPRTVRVLNRDDAEV
MRFAPPAAAADAPRAVTFGLNEPAADGDYGLLRENGIAWLVEAIDRDGADAPAAPSRRRKQEAANPPDIALKRLMPADAL
RIRGLHNAANALAAYALARAIGLPAAPLLHGLREYRGEPHRVEVIATLDGVDYVDDSKGTNVGATVAALDGLAQRAVLIA
GGDGKGQDFEPLAAPVARWCRAVMLIGRDAPALREALADTGVPLADHATLEAAVRAASALAQPGDAVLLSPACASLDMFR
NYAHRADVFRSAVEDIALEKGTTL

Specific function: Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA)

COG id: COG0771

COG function: function code M; UDP-N-acetylmuramoylalanine-D-glutamate ligase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family

Homologues:

Organism=Escherichia coli, GI1786276, Length=493, Percent_Identity=38.5395537525355, Blast_Score=235, Evalue=5e-63,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MURD_BURM7 (A3MR61)

Other databases:

- EMBL:   CP000548
- RefSeq:   YP_001082747.1
- ProteinModelPortal:   A3MR61
- SMR:   A3MR61
- GeneID:   4893750
- GenomeReviews:   CP000548_GR
- KEGG:   bmn:BMA10247_3230
- TIGR:   BMA10247_3230
- HOGENOM:   HBG750024
- OMA:   VKYYNDS
- ProtClustDB:   PRK02006
- BioCyc:   BMAL320389:BMA10247_3230-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00639
- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR016040
- InterPro:   IPR005762
- Gene3D:   G3DSA:3.90.190.20
- Gene3D:   G3DSA:3.40.1190.10
- Gene3D:   G3DSA:3.40.50.720
- TIGRFAMs:   TIGR01087

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M; SSF53244 Mur_ligase_C; SSF53623 Mur_ligase_cen

EC number: =6.3.2.9

Molecular weight: Translated: 52549; Mature: 52549

Theoretical pI: Translated: 5.21; Mature: 5.21

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFGDRQRPMVLVLGLGESGLAIARWCARHGCRLRVADTRETPPNLAALTAAGVDFEFVGG
CCCCCCCCEEEEEECCCCCHHHHHHHHHCCCEEEECCCCCCCCCCEEEEECCCCEEECCC
AFSPALVDGGIELVALSPGLSPLAEDLAPLVAAARERGIPVWGELEFFAQALAALGANGY
CCCCHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCC
APKVIAITGTNGKTTTTSLAGLLCERAGKKVAVAGNISPAMLDKLTEAIDAAALPDVWVL
CCEEEEEECCCCCCHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHCCCCEEEE
ELSSFQLDTAHTFAPDAATILNITQDHLDWHGGFAAYAAAKGRVFGPRTVRVLNRDDAEV
EECCEEECCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHCCCCEECCCEEEEECCCCHHH
MRFAPPAAAADAPRAVTFGLNEPAADGDYGLLRENGIAWLVEAIDRDGADAPAAPSRRRK
HEECCCCCCCCCCCEEEECCCCCCCCCCCCEECCCCHHHHHHHHCCCCCCCCCCCHHHHH
QEAANPPDIALKRLMPADALRIRGLHNAANALAAYALARAIGLPAAPLLHGLREYRGEPH
HHCCCCHHHHHHHHCCHHHHHHCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCC
RVEVIATLDGVDYVDDSKGTNVGATVAALDGLAQRAVLIAGGDGKGQDFEPLAAPVARWC
EEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHCEEEEECCCCCCCCCCHHHHHHHHHH
RAVMLIGRDAPALREALADTGVPLADHATLEAAVRAASALAQPGDAVLLSPACASLDMFR
HHHHHHCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHH
NYAHRADVFRSAVEDIALEKGTTL
HHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MFGDRQRPMVLVLGLGESGLAIARWCARHGCRLRVADTRETPPNLAALTAAGVDFEFVGG
CCCCCCCCEEEEEECCCCCHHHHHHHHHCCCEEEECCCCCCCCCCEEEEECCCCEEECCC
AFSPALVDGGIELVALSPGLSPLAEDLAPLVAAARERGIPVWGELEFFAQALAALGANGY
CCCCHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCC
APKVIAITGTNGKTTTTSLAGLLCERAGKKVAVAGNISPAMLDKLTEAIDAAALPDVWVL
CCEEEEEECCCCCCHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHCCCCEEEE
ELSSFQLDTAHTFAPDAATILNITQDHLDWHGGFAAYAAAKGRVFGPRTVRVLNRDDAEV
EECCEEECCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHCCCCEECCCEEEEECCCCHHH
MRFAPPAAAADAPRAVTFGLNEPAADGDYGLLRENGIAWLVEAIDRDGADAPAAPSRRRK
HEECCCCCCCCCCCEEEECCCCCCCCCCCCEECCCCHHHHHHHHCCCCCCCCCCCHHHHH
QEAANPPDIALKRLMPADALRIRGLHNAANALAAYALARAIGLPAAPLLHGLREYRGEPH
HHCCCCHHHHHHHHCCHHHHHHCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCC
RVEVIATLDGVDYVDDSKGTNVGATVAALDGLAQRAVLIAGGDGKGQDFEPLAAPVARWC
EEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHCEEEEECCCCCCCCCCHHHHHHHHHH
RAVMLIGRDAPALREALADTGVPLADHATLEAAVRAASALAQPGDAVLLSPACASLDMFR
HHHHHHCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHH
NYAHRADVFRSAVEDIALEKGTTL
HHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA