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The map label for this gene is hgiDIIM [H]

Identifier: 126453573

GI number: 126453573

Start: 3597115

End: 3598149

Strand: Reverse

Name: hgiDIIM [H]

Synonym: BURPS1106A_3681

Alternate gene names: 126453573

Gene position: 3598149-3597115 (Counterclockwise)

Preceding gene: 126454476

Following gene: 126452260

Centisome position: 90.21

GC content: 57.58

Gene sequence:

>1035_bases
GTGGCGAATATTTCTTGTGTCGACTTGTTCTGCGGGGCGGGCGGGCTGACGCATGGTTTCGTTTTGGAGGATTTGCCCGT
GGTCGCAGGTATCGACCTGGACCCGGCATGCCGCTATCCGTACGAAGCGAACAACCGAGCCAAGTTCGTAGAGCGGGACG
TCAGCACGGTTACCACTGAAGAGTTAGAAACGTTGTTTGGCGACGCAGAGCTGACGATCCTTGCAGGCTGTGCGCCATGT
CAACCTTTCTCGAGTTACGCCCAGCGTTATGAACTTGATGGCGAAGATGGCAAGTGGGGTTTGCTGTACGAGTTTGCTCG
TCTTGCGCAGGGCACAGAACCCGATGTCATCACGATGGAAAACGTTCCTACCGTTGCTAAGCATGCTGTTTTCCATGACT
TCGTCGATACGCTGACCCGACTGGGGTACAAGGTGTGGTTCGACGTGGTCGACAGCTCGCGCTATGGTGTGCCGCAGATG
AGGCGGCGTATGGTACTGCTCGCATCGAAGCATGGCGATATCGAGATGATCGAGCCGACGCACAAAACGCCGAAGACAGT
GCGCCAGGCCATTGGTCGACTGCGTTCTTTGTCGGCAGGGGAGGCGGCCCCTAGGGACAAGTTGCACGTCGCGTCGACTC
TATCAGAGAAGAATCTGCAGCGGATCAAGGTATCGAAACCAGGGGGGACATGGCGCGATTGGCCGGAAGACCTCATCGCT
GAATGCCATCGTGCCGAGAGCGGCCGTACCTACCCAGGCGTCTATGGTCGGATGGAGTGGGACAAGCCAGCTCCTACGAT
GACGACTCAGTGTTATGGCTTCGGAAACGGACGGTTTGGCCACCCCGAGCAGGACCGCGCAATTTCGCTGAGGGAAGCGG
CAATTCTGCAAAGCTTCCCGCGCGACTACGCATTCGTTCCGAAGGATGGCGAGGTCAGTTTCACGGTGTTAGGGCGCCTG
ATCGGCAATGCGGTGCCGGTAAACCTGGGTCGCGCCATCGCACGGAGTATCAAGGAACATCTTGCATCGATCTAG

Upstream 100 bases:

>100_bases
CGCGGCCGAGCACGATGGTCGGGTCAAGGAGTGAAAGCGTTTTCTGCGGTCGGTTTCAATAGCGCCATAGGCTGCTTGAG
ACGCAATGAGGAGGTTTGAA

Downstream 100 bases:

>100_bases
CTTAACGGCTGATAGCTGAGAGAGCGTCCATGGCTTTCCGCGACCCACCGTCGACTGAAATCAGTCGCCGCATGGCGAAG
GTTCGGCAAAAGGCAACCGA

Product: modification methylase HgiDII

Products: NA

Alternate protein names: M.HgiDII; Cytosine-specific methyltransferase HgiDII [H]

Number of amino acids: Translated: 344; Mature: 343

Protein sequence:

>344_residues
MANISCVDLFCGAGGLTHGFVLEDLPVVAGIDLDPACRYPYEANNRAKFVERDVSTVTTEELETLFGDAELTILAGCAPC
QPFSSYAQRYELDGEDGKWGLLYEFARLAQGTEPDVITMENVPTVAKHAVFHDFVDTLTRLGYKVWFDVVDSSRYGVPQM
RRRMVLLASKHGDIEMIEPTHKTPKTVRQAIGRLRSLSAGEAAPRDKLHVASTLSEKNLQRIKVSKPGGTWRDWPEDLIA
ECHRAESGRTYPGVYGRMEWDKPAPTMTTQCYGFGNGRFGHPEQDRAISLREAAILQSFPRDYAFVPKDGEVSFTVLGRL
IGNAVPVNLGRAIARSIKEHLASI

Sequences:

>Translated_344_residues
MANISCVDLFCGAGGLTHGFVLEDLPVVAGIDLDPACRYPYEANNRAKFVERDVSTVTTEELETLFGDAELTILAGCAPC
QPFSSYAQRYELDGEDGKWGLLYEFARLAQGTEPDVITMENVPTVAKHAVFHDFVDTLTRLGYKVWFDVVDSSRYGVPQM
RRRMVLLASKHGDIEMIEPTHKTPKTVRQAIGRLRSLSAGEAAPRDKLHVASTLSEKNLQRIKVSKPGGTWRDWPEDLIA
ECHRAESGRTYPGVYGRMEWDKPAPTMTTQCYGFGNGRFGHPEQDRAISLREAAILQSFPRDYAFVPKDGEVSFTVLGRL
IGNAVPVNLGRAIARSIKEHLASI
>Mature_343_residues
ANISCVDLFCGAGGLTHGFVLEDLPVVAGIDLDPACRYPYEANNRAKFVERDVSTVTTEELETLFGDAELTILAGCAPCQ
PFSSYAQRYELDGEDGKWGLLYEFARLAQGTEPDVITMENVPTVAKHAVFHDFVDTLTRLGYKVWFDVVDSSRYGVPQMR
RRMVLLASKHGDIEMIEPTHKTPKTVRQAIGRLRSLSAGEAAPRDKLHVASTLSEKNLQRIKVSKPGGTWRDWPEDLIAE
CHRAESGRTYPGVYGRMEWDKPAPTMTTQCYGFGNGRFGHPEQDRAISLREAAILQSFPRDYAFVPKDGEVSFTVLGRLI
GNAVPVNLGRAIARSIKEHLASI

Specific function: This methylase recognizes the double-stranded sequence GTCGAC, causes specific methylation on C-? on both strands and protects the DNA from cleavage by the HgiDII endonuclease [H]

COG id: COG0270

COG function: function code L; Site-specific DNA methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the C5-methyltransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018117
- InterPro:   IPR001525 [H]

Pfam domain/function: PF00145 DNA_methylase [H]

EC number: =2.1.1.37 [H]

Molecular weight: Translated: 38164; Mature: 38033

Theoretical pI: Translated: 6.42; Mature: 6.42

Prosite motif: PS00095 C5_MTASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANISCVDLFCGAGGLTHGFVLEDLPVVAGIDLDPACRYPYEANNRAKFVERDVSTVTTE
CCCCEEEHHHHCCCCCCCCHHHHCCCEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
ELETLFGDAELTILAGCAPCQPFSSYAQRYELDGEDGKWGLLYEFARLAQGTEPDVITME
HHHHHHCCCCEEEEECCCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCEEEEC
NVPTVAKHAVFHDFVDTLTRLGYKVWFDVVDSSRYGVPQMRRRMVLLASKHGDIEMIEPT
CCCHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCHHHHHHEEEEECCCCCEEEECCC
HKTPKTVRQAIGRLRSLSAGEAAPRDKLHVASTLSEKNLQRIKVSKPGGTWRDWPEDLIA
CCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCHHEEEEECCCCCCCCCHHHHHH
ECHRAESGRTYPGVYGRMEWDKPAPTMTTQCYGFGNGRFGHPEQDRAISLREAAILQSFP
HHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHCC
RDYAFVPKDGEVSFTVLGRLIGNAVPVNLGRAIARSIKEHLASI
CCCEECCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
ANISCVDLFCGAGGLTHGFVLEDLPVVAGIDLDPACRYPYEANNRAKFVERDVSTVTTE
CCCEEEHHHHCCCCCCCCHHHHCCCEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
ELETLFGDAELTILAGCAPCQPFSSYAQRYELDGEDGKWGLLYEFARLAQGTEPDVITME
HHHHHHCCCCEEEEECCCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCEEEEC
NVPTVAKHAVFHDFVDTLTRLGYKVWFDVVDSSRYGVPQMRRRMVLLASKHGDIEMIEPT
CCCHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCHHHHHHEEEEECCCCCEEEECCC
HKTPKTVRQAIGRLRSLSAGEAAPRDKLHVASTLSEKNLQRIKVSKPGGTWRDWPEDLIA
CCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCHHEEEEECCCCCCCCCHHHHHH
ECHRAESGRTYPGVYGRMEWDKPAPTMTTQCYGFGNGRFGHPEQDRAISLREAAILQSFP
HHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHCC
RDYAFVPKDGEVSFTVLGRLIGNAVPVNLGRAIARSIKEHLASI
CCCEECCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1937045; 7607523 [H]