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The map label for this gene is iolD [H]

Identifier: 126453463

GI number: 126453463

Start: 1629377

End: 1631317

Strand: Reverse

Name: iolD [H]

Synonym: BURPS1106A_1676

Alternate gene names: 126453463

Gene position: 1631317-1629377 (Counterclockwise)

Preceding gene: 126452179

Following gene: 126454709

Centisome position: 40.9

GC content: 73.52

Gene sequence:

>1941_bases
GTGAACGACTTCATCGACGATCCGGCCGCGAGCGTGCAACGCACGGTCCGGCTGACCGCCGCGCAGGCGCTCGTGCGCTA
TCTGGCCGCGCAGCGCGTCGCGACCGGCGACGGCGACGCGCCGCTGTTCGGCGGCGTGTTCGCGATCTTCGGGCACGGCA
ACGTCGCGGGCCTGGGCGAGGCGCTGCATCAATACCGCGACGCGCTGCCGACCTACCGCGCGCACAACGAGCAGGCGATG
GCGCACAGCGCGATCGCGTACGCGAAGGCGCACCTGCGCCGCCGGATGATGGCCGTGACGACATCGATCGGCCCCGGCGC
GACGAACCTCGTCACCGCGGCCGCGCTCGCGCACGTGAACCGGCTGCCGGTGCTGCTGCTGCCGGGCGACGTGTTCGTGT
CGCGCGCGCCGGACCCGGTGCTGCAGCAGATCGAGGATTTCCATGACGGCGCGATCTCGGCGAACGACGCGTTCAAGGCC
GTGTCGCGCTACTTCGACCGGATCGTGCATCCGGCCCAGTTGCTCACCGCGCTGCCGCGCGCGCTGCGCGTGCTGACCGA
CGCCGCGCTCTGCGGCCCCGTGACGCTCGCGCTGCCGCAGGACGTGCAGACGATGGCCTACGACTACCCGGCCGAGTTCT
TCGCGCCGCGCGCCGTCACGTTCCATGCGCCCGCGCCCGTCGAGGCGGAGCTCGACGCGGCGCTCGCGCCACTGCGCGCG
TCGGCCCGGCCGTTCATCGTCGCGGGCGGCGGCGCGCTATACAGCGAAGGCGGCGCGGCCGCGCTCGCCGCGTTCGCCGA
GCGCCACGGCATCCCGGTCGGCGAGACGCAGGCCGGCAAGAGCGCACTGCCGTGGGACCACCCGCTGAACGCCGGCGCGA
TCGGCGTGACGGGCTCGCCCGCGGCGAACGCGCTCGCGCACGACGCCGATTGCGTGATCGCGGTCGGCACGCGGCTGCAG
GATTTCACGACCGGCTCGAACACGCTGTTCGCGCGCGCACGCGTCATCGGCATCAACGCGAACGCGTTCGATGCGCTCAA
GCATCGCGCGACGATCGTGCAGGCGGACGCGCGGCTCGCGCTCGACGCGCTCGGCGCGCGCCTCGAAGGCTGGCGCGCCG
ACGCCGCGTGGACGGCGCGCGCGCACGCGCGCGCGAACGCATGGCGCGACGCCGTCGCGGCCGTCACGCATGCGCCGCCG
CGCGCGCGGGCGCTGCCGTACGACGCCGACGTGATCGGCGCCGTGCAGCGCTCGGCGCCCGATTCGGCCGCGCGCGACAT
CGCCGTGTGCGCGGCGGGCACGCTGCCCGCCGAGCTGCACAAGCTGTGGCGCGCGGCGGCGCCGGGCGGCTATCACGTCG
AATACGGCTATTCGTGCATGGGCTACGAGATCGCGGGCGGGCTCGGCGCGAAGCTCGCGAAGCCCGAGCGCGAGGTGATC
GTGATGGTCGGCGACGGCAGCTATCTGATGATGAACAGCGAGATCGCGACGTCGGTGATGCTCGGCGCGAAGCTGATCGT
CGTGGTGCTCGACAACCGCGGCTACGGCTGCATCAACCGGCTGCAGCAGGCGTGCGGCGGCGCGCCGTTCAACAACCTGT
TCGACGACTGCGTGCAAGGCCCGCTCGGCGCGCCGCGCATCGATTTCGCCGCGCACGCGCGCGCGCTCGGCGCGCGCGCG
GAGCACGTCGCCGATATCGCCGCGCTCGAAGCGGCGATGGCGCGCGCCCGCGCGTCCGATCGCACGTACGTGATCTGCAT
CGACACCGATCCCGCCCGCACGACGCCCGACGGCGGCTGGTGGTGGGAAGTCGCGGTGCCCGAAGTGTCGCCGCGCGACG
CGGTGCGCGACGCGCGCGCGGATTACGAGCGGCACCTCGCCGCGCGCGCGGGCGCTCCCGCCTCTCGTTCAACCCCCTCT
ACTCCGGAATCATCGACATGA

Upstream 100 bases:

>100_bases
GCGCGCGGCCGCCACGCCGGCGCCGTGCCGCGCGTGGCCGCCTAGGAGCCGCGCGATGAATCGCAGAGCAATGGATGACG
ACACCCGACGGAGCCCGAGC

Downstream 100 bases:

>100_bases
GCGCACCCGACATCCGTATCGGCATCAACCCGCTGTCGTGGATGAACGACGATCTGCCGTCGCTCGGCGGCGAGACGCCG
CTCGCCGTCGCGCTGACCGA

Product: iolD protein

Products: pyruvate; 2-aceto-2-hydroxy-butyrate; CO2 [C]

Alternate protein names: THcHDO hydrolase [H]

Number of amino acids: Translated: 646; Mature: 646

Protein sequence:

>646_residues
MNDFIDDPAASVQRTVRLTAAQALVRYLAAQRVATGDGDAPLFGGVFAIFGHGNVAGLGEALHQYRDALPTYRAHNEQAM
AHSAIAYAKAHLRRRMMAVTTSIGPGATNLVTAAALAHVNRLPVLLLPGDVFVSRAPDPVLQQIEDFHDGAISANDAFKA
VSRYFDRIVHPAQLLTALPRALRVLTDAALCGPVTLALPQDVQTMAYDYPAEFFAPRAVTFHAPAPVEAELDAALAPLRA
SARPFIVAGGGALYSEGGAAALAAFAERHGIPVGETQAGKSALPWDHPLNAGAIGVTGSPAANALAHDADCVIAVGTRLQ
DFTTGSNTLFARARVIGINANAFDALKHRATIVQADARLALDALGARLEGWRADAAWTARAHARANAWRDAVAAVTHAPP
RARALPYDADVIGAVQRSAPDSAARDIAVCAAGTLPAELHKLWRAAAPGGYHVEYGYSCMGYEIAGGLGAKLAKPEREVI
VMVGDGSYLMMNSEIATSVMLGAKLIVVVLDNRGYGCINRLQQACGGAPFNNLFDDCVQGPLGAPRIDFAAHARALGARA
EHVADIAALEAAMARARASDRTYVICIDTDPARTTPDGGWWWEVAVPEVSPRDAVRDARADYERHLAARAGAPASRSTPS
TPESST

Sequences:

>Translated_646_residues
MNDFIDDPAASVQRTVRLTAAQALVRYLAAQRVATGDGDAPLFGGVFAIFGHGNVAGLGEALHQYRDALPTYRAHNEQAM
AHSAIAYAKAHLRRRMMAVTTSIGPGATNLVTAAALAHVNRLPVLLLPGDVFVSRAPDPVLQQIEDFHDGAISANDAFKA
VSRYFDRIVHPAQLLTALPRALRVLTDAALCGPVTLALPQDVQTMAYDYPAEFFAPRAVTFHAPAPVEAELDAALAPLRA
SARPFIVAGGGALYSEGGAAALAAFAERHGIPVGETQAGKSALPWDHPLNAGAIGVTGSPAANALAHDADCVIAVGTRLQ
DFTTGSNTLFARARVIGINANAFDALKHRATIVQADARLALDALGARLEGWRADAAWTARAHARANAWRDAVAAVTHAPP
RARALPYDADVIGAVQRSAPDSAARDIAVCAAGTLPAELHKLWRAAAPGGYHVEYGYSCMGYEIAGGLGAKLAKPEREVI
VMVGDGSYLMMNSEIATSVMLGAKLIVVVLDNRGYGCINRLQQACGGAPFNNLFDDCVQGPLGAPRIDFAAHARALGARA
EHVADIAALEAAMARARASDRTYVICIDTDPARTTPDGGWWWEVAVPEVSPRDAVRDARADYERHLAARAGAPASRSTPS
TPESST
>Mature_646_residues
MNDFIDDPAASVQRTVRLTAAQALVRYLAAQRVATGDGDAPLFGGVFAIFGHGNVAGLGEALHQYRDALPTYRAHNEQAM
AHSAIAYAKAHLRRRMMAVTTSIGPGATNLVTAAALAHVNRLPVLLLPGDVFVSRAPDPVLQQIEDFHDGAISANDAFKA
VSRYFDRIVHPAQLLTALPRALRVLTDAALCGPVTLALPQDVQTMAYDYPAEFFAPRAVTFHAPAPVEAELDAALAPLRA
SARPFIVAGGGALYSEGGAAALAAFAERHGIPVGETQAGKSALPWDHPLNAGAIGVTGSPAANALAHDADCVIAVGTRLQ
DFTTGSNTLFARARVIGINANAFDALKHRATIVQADARLALDALGARLEGWRADAAWTARAHARANAWRDAVAAVTHAPP
RARALPYDADVIGAVQRSAPDSAARDIAVCAAGTLPAELHKLWRAAAPGGYHVEYGYSCMGYEIAGGLGAKLAKPEREVI
VMVGDGSYLMMNSEIATSVMLGAKLIVVVLDNRGYGCINRLQQACGGAPFNNLFDDCVQGPLGAPRIDFAAHARALGARA
EHVADIAALEAAMARARASDRTYVICIDTDPARTTPDGGWWWEVAVPEVSPRDAVRDARADYERHLAARAGAPASRSTPS
TPESST

Specific function: Involved in the cleavage of the C1-C2 bond of 3D- (3,5/4)-trihydroxycyclohexane-1,2-dione (THcHDO) to yield 5-deoxy- glucuronate (5DG) [H]

COG id: COG3962

COG function: function code E; Acetolactate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Escherichia coli, GI87081685, Length=535, Percent_Identity=25.7943925233645, Blast_Score=119, Evalue=6e-28,
Organism=Escherichia coli, GI1790104, Length=525, Percent_Identity=25.5238095238095, Blast_Score=118, Evalue=1e-27,
Organism=Escherichia coli, GI1786717, Length=537, Percent_Identity=26.6294227188082, Blast_Score=106, Evalue=6e-24,
Organism=Escherichia coli, GI1787096, Length=491, Percent_Identity=23.8289205702648, Blast_Score=82, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6323755, Length=607, Percent_Identity=23.0642504118616, Blast_Score=123, Evalue=8e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: 2.2.1.6 [C]

Molecular weight: Translated: 67903; Mature: 67903

Theoretical pI: Translated: 6.86; Mature: 6.86

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDFIDDPAASVQRTVRLTAAQALVRYLAAQRVATGDGDAPLFGGVFAIFGHGNVAGLGE
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHCCEEEEECCCCHHHHHH
ALHQYRDALPTYRAHNEQAMAHSAIAYAKAHLRRRMMAVTTSIGPGATNLVTAAALAHVN
HHHHHHHCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHC
RLPVLLLPGDVFVSRAPDPVLQQIEDFHDGAISANDAFKAVSRYFDRIVHPAQLLTALPR
CCCEEEECCHHEECCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHH
ALRVLTDAALCGPVTLALPQDVQTMAYDYPAEFFAPRAVTFHAPAPVEAELDAALAPLRA
HHHHHHHHHHHCCEEEECCCCHHHHHHCCCHHHCCCCEEEEECCCCCCHHHHHHHHHHHC
SARPFIVAGGGALYSEGGAAALAAFAERHGIPVGETQAGKSALPWDHPLNAGAIGVTGSP
CCCCEEEECCCEEECCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCC
AANALAHDADCVIAVGTRLQDFTTGSNTLFARARVIGINANAFDALKHRATIVQADARLA
HHHHHHCCCCEEEEECCCHHHCCCCCCEEEEEEEEEEECCCHHHHHHHHHHEEECHHHHH
LDALGARLEGWRADAAWTARAHARANAWRDAVAAVTHAPPRARALPYDADVIGAVQRSAP
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCC
DSAARDIAVCAAGTLPAELHKLWRAAAPGGYHVEYGYSCMGYEIAGGLGAKLAKPEREVI
CHHHHCEEEEECCCCHHHHHHHHHHCCCCCEEEEECCEEECEEECCCCCCCCCCCCCEEE
VMVGDGSYLMMNSEIATSVMLGAKLIVVVLDNRGYGCINRLQQACGGAPFNNLFDDCVQG
EEECCCCEEEECHHHHHHHHHCCEEEEEEECCCCCHHHHHHHHHHCCCCHHHHHHHHHCC
PLGAPRIDFAAHARALGARAEHVADIAALEAAMARARASDRTYVICIDTDPARTTPDGGW
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCE
WWEVAVPEVSPRDAVRDARADYERHLAARAGAPASRSTPSTPESST
EEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MNDFIDDPAASVQRTVRLTAAQALVRYLAAQRVATGDGDAPLFGGVFAIFGHGNVAGLGE
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHCCEEEEECCCCHHHHHH
ALHQYRDALPTYRAHNEQAMAHSAIAYAKAHLRRRMMAVTTSIGPGATNLVTAAALAHVN
HHHHHHHCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHC
RLPVLLLPGDVFVSRAPDPVLQQIEDFHDGAISANDAFKAVSRYFDRIVHPAQLLTALPR
CCCEEEECCHHEECCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHH
ALRVLTDAALCGPVTLALPQDVQTMAYDYPAEFFAPRAVTFHAPAPVEAELDAALAPLRA
HHHHHHHHHHHCCEEEECCCCHHHHHHCCCHHHCCCCEEEEECCCCCCHHHHHHHHHHHC
SARPFIVAGGGALYSEGGAAALAAFAERHGIPVGETQAGKSALPWDHPLNAGAIGVTGSP
CCCCEEEECCCEEECCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCC
AANALAHDADCVIAVGTRLQDFTTGSNTLFARARVIGINANAFDALKHRATIVQADARLA
HHHHHHCCCCEEEEECCCHHHCCCCCCEEEEEEEEEEECCCHHHHHHHHHHEEECHHHHH
LDALGARLEGWRADAAWTARAHARANAWRDAVAAVTHAPPRARALPYDADVIGAVQRSAP
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCC
DSAARDIAVCAAGTLPAELHKLWRAAAPGGYHVEYGYSCMGYEIAGGLGAKLAKPEREVI
CHHHHCEEEEECCCCHHHHHHHHHHCCCCCEEEEECCEEECEEECCCCCCCCCCCCCEEE
VMVGDGSYLMMNSEIATSVMLGAKLIVVVLDNRGYGCINRLQQACGGAPFNNLFDDCVQG
EEECCCCEEEECHHHHHHHHHCCEEEEEEECCCCCHHHHHHHHHHCCCCHHHHHHHHHCC
PLGAPRIDFAAHARALGARAEHVADIAALEAAMARARASDRTYVICIDTDPARTTPDGGW
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCE
WWEVAVPEVSPRDAVRDARADYERHLAARAGAPASRSTPSTPESST
EEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: FAD [C]

Metal ions: Mg2+ [C]

Kcat value (1/min): 4002 [C]

Specific activity: 53

Km value (mM): 5 {2-oxobutanoate}} 1 {2-oxobutanoate}} 7.6 {pyruvate}} 7 {pyruvate}} 2.9 {pyruvate}} 2.6 {pyruvate}} 1.3 {pyruvate}} 1 {pyruvate}} [C]

Substrates: acetolactate; CO2; pyruvate; 2-oxobutanoate [C]

Specific reaction: acetolactate + CO2 = 2 pyruvate pyruvate + 2-oxobutanoate = 2-aceto-2-hydroxy-butyrate + CO2 [C]

General reaction: C-C bond formation; decarboxylation [C]

Inhibitor: @OXOB02.txt*2-oxobutanoate!; chlorimuronethyl; chlorsulphuron; Ile; imazapyr; Leu; Val [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA