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The map label for this gene is dusB [H]

Identifier: 126452924

GI number: 126452924

Start: 3314558

End: 3315622

Strand: Direct

Name: dusB [H]

Synonym: BURPS1106A_3396

Alternate gene names: 126452924

Gene position: 3314558-3315622 (Clockwise)

Preceding gene: 126454612

Following gene: 126451778

Centisome position: 83.1

GC content: 67.79

Gene sequence:

>1065_bases
ATGCCCGTCCTAGGCTCTCACGTCCTGCGCAACAATCTGTTCGTCGCCCCGATGGCGGGCGTCACGGACCGGCCGTTCCG
CCAGCTGTGCAAGCGGCTCGGCGCGGGCTATGCGGTGTCGGAGATGGTCGCGTCGAACGCGCAGCTGTGGAAGAGCGAGA
AGACGATGCGCCGCGCGAACCACGCGGGCGAGGTCGAGCCGATCGCGGTGCAGATCGCGGGCGCCGACCCGGCGATGATG
GCCGAGGCCGCGCGTCACAACGTCGCGAACGGCGCGCAGATCATCGACATCAACATGGGTTGCCCGGCGAAGAAGGTCTG
CAACGTCGCCGCCGGCTCGGCGCTGCTGCAGAACGAGCCGCTCGTGCAGCGGATCGTCGAGGCGGTCGTCGGCGCGGTGG
GCGCGGGCGCCGACGCCGTGCCCGTCACGCTGAAGATCCGCACCGGCTGGGACCGCGATCACAAGAACGCGCTCAGGATC
GCGACGCTCGCCGAGGCGGCCGGCATCTCGATGCTGACCGTGCACGGCCGCACCCGCGCGGACCTGTACCGCGGCGACGC
CGAATACGACACGATCGCGGCCGTGAAAGCCGCCGTGCGCATTCCGGTCGTCGCGAACGGCGACATCACGTCGCCGCGAA
AGGCGAAGGCCGTGCTCGACGCGACGCGCGCCGACGCGCTGATGATCGGCCGCGCCGCGCAAGGAAGGCCGTGGCTCTTC
CGCGAAATCGATCATTTCCTGCAAAGCGGCGAGCTTCTGCCGCCGCCGCGCATCGACGAGATCCGCCAGGTGATGAACGA
ACACCTGGAGGATCACTACGCGTTCTACGGCGAATTCACGGGAGTCCGTACTGCACGCAAGCACATCGGCTGGTACACTC
GCGGCCTTTCCGGCGCGAACGGGTTCCGGCACCGAATGAACACGCTCGAAACCACCCGCGAGCAGCTTGCCGCCGTCAAC
GAATTCTTCGACGCGCAACAGGCGCTGTCCGATCGCCTCGTCTATGTGGACGACGAAGACGGCGACCGCGGCGAGCCGGA
CGATTCTAACCAGTTAGCAGCATGA

Upstream 100 bases:

>100_bases
GACAGTTGCCTAATTCGGCGGCACCGGCGGTTACGGTAAAATACGCGTTTTCCCTTTCGCCGACTCCCCGCCCGCGCCTC
GCGCGGGCGCTCCCATTGCG

Downstream 100 bases:

>100_bases
GCAAGCACAACATCGAACAATGTGTCCGCGACAGCCTGGACGGCTACTTCCGGGATCTCGACGGCTCCAATCCGCACGAC
GTCTATGAAATGGTGATGTC

Product: tRNA-dihydrouridine synthase B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 354; Mature: 353

Protein sequence:

>354_residues
MPVLGSHVLRNNLFVAPMAGVTDRPFRQLCKRLGAGYAVSEMVASNAQLWKSEKTMRRANHAGEVEPIAVQIAGADPAMM
AEAARHNVANGAQIIDINMGCPAKKVCNVAAGSALLQNEPLVQRIVEAVVGAVGAGADAVPVTLKIRTGWDRDHKNALRI
ATLAEAAGISMLTVHGRTRADLYRGDAEYDTIAAVKAAVRIPVVANGDITSPRKAKAVLDATRADALMIGRAAQGRPWLF
REIDHFLQSGELLPPPRIDEIRQVMNEHLEDHYAFYGEFTGVRTARKHIGWYTRGLSGANGFRHRMNTLETTREQLAAVN
EFFDAQQALSDRLVYVDDEDGDRGEPDDSNQLAA

Sequences:

>Translated_354_residues
MPVLGSHVLRNNLFVAPMAGVTDRPFRQLCKRLGAGYAVSEMVASNAQLWKSEKTMRRANHAGEVEPIAVQIAGADPAMM
AEAARHNVANGAQIIDINMGCPAKKVCNVAAGSALLQNEPLVQRIVEAVVGAVGAGADAVPVTLKIRTGWDRDHKNALRI
ATLAEAAGISMLTVHGRTRADLYRGDAEYDTIAAVKAAVRIPVVANGDITSPRKAKAVLDATRADALMIGRAAQGRPWLF
REIDHFLQSGELLPPPRIDEIRQVMNEHLEDHYAFYGEFTGVRTARKHIGWYTRGLSGANGFRHRMNTLETTREQLAAVN
EFFDAQQALSDRLVYVDDEDGDRGEPDDSNQLAA
>Mature_353_residues
PVLGSHVLRNNLFVAPMAGVTDRPFRQLCKRLGAGYAVSEMVASNAQLWKSEKTMRRANHAGEVEPIAVQIAGADPAMMA
EAARHNVANGAQIIDINMGCPAKKVCNVAAGSALLQNEPLVQRIVEAVVGAVGAGADAVPVTLKIRTGWDRDHKNALRIA
TLAEAAGISMLTVHGRTRADLYRGDAEYDTIAAVKAAVRIPVVANGDITSPRKAKAVLDATRADALMIGRAAQGRPWLFR
EIDHFLQSGELLPPPRIDEIRQVMNEHLEDHYAFYGEFTGVRTARKHIGWYTRGLSGANGFRHRMNTLETTREQLAAVNE
FFDAQQALSDRLVYVDDEDGDRGEPDDSNQLAA

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family. DusB subfamily [H]

Homologues:

Organism=Homo sapiens, GI239788483, Length=285, Percent_Identity=31.2280701754386, Blast_Score=117, Evalue=1e-26,
Organism=Homo sapiens, GI239788462, Length=289, Percent_Identity=31.8339100346021, Blast_Score=115, Evalue=4e-26,
Organism=Homo sapiens, GI31742496, Length=228, Percent_Identity=32.0175438596491, Blast_Score=101, Evalue=9e-22,
Organism=Homo sapiens, GI40807366, Length=233, Percent_Identity=32.618025751073, Blast_Score=93, Evalue=4e-19,
Organism=Homo sapiens, GI8923374, Length=255, Percent_Identity=27.0588235294118, Blast_Score=69, Evalue=5e-12,
Organism=Escherichia coli, GI1789660, Length=321, Percent_Identity=56.6978193146417, Blast_Score=361, Evalue=1e-101,
Organism=Escherichia coli, GI1788462, Length=241, Percent_Identity=31.5352697095436, Blast_Score=108, Evalue=5e-25,
Organism=Escherichia coli, GI145693211, Length=243, Percent_Identity=29.2181069958848, Blast_Score=80, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17543114, Length=286, Percent_Identity=27.972027972028, Blast_Score=97, Evalue=9e-21,
Organism=Caenorhabditis elegans, GI25144369, Length=224, Percent_Identity=30.8035714285714, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17507177, Length=235, Percent_Identity=31.063829787234, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17510279, Length=254, Percent_Identity=28.3464566929134, Blast_Score=74, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6323433, Length=320, Percent_Identity=26.875, Blast_Score=92, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6323560, Length=267, Percent_Identity=28.0898876404494, Blast_Score=79, Evalue=8e-16,
Organism=Saccharomyces cerevisiae, GI6323437, Length=228, Percent_Identity=27.1929824561404, Blast_Score=75, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24585320, Length=288, Percent_Identity=30.9027777777778, Blast_Score=120, Evalue=1e-27,
Organism=Drosophila melanogaster, GI24580595, Length=231, Percent_Identity=31.6017316017316, Blast_Score=98, Evalue=8e-21,
Organism=Drosophila melanogaster, GI19920448, Length=231, Percent_Identity=31.6017316017316, Blast_Score=98, Evalue=8e-21,
Organism=Drosophila melanogaster, GI19921524, Length=229, Percent_Identity=27.9475982532751, Blast_Score=86, Evalue=3e-17,
Organism=Drosophila melanogaster, GI45549423, Length=259, Percent_Identity=30.1158301158301, Blast_Score=70, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004652
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 38492; Mature: 38361

Theoretical pI: Translated: 7.07; Mature: 7.07

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPVLGSHVLRNNLFVAPMAGVTDRPFRQLCKRLGAGYAVSEMVASNAQLWKSEKTMRRAN
CCCCHHHHHHCCEEEEECCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHC
HAGEVEPIAVQIAGADPAMMAEAARHNVANGAQIIDINMGCPAKKVCNVAAGSALLQNEP
CCCCCCEEEEEEECCCHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHCHHHHCCCH
LVQRIVEAVVGAVGAGADAVPVTLKIRTGWDRDHKNALRIATLAEAAGISMLTVHGRTRA
HHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCHHHHHHHHHHCCCEEEEECCCCHH
DLYRGDAEYDTIAAVKAAVRIPVVANGDITSPRKAKAVLDATRADALMIGRAAQGRPWLF
HHHCCCCCHHHHHHHHHHHEEEEEECCCCCCCHHHHHHHHHHHHCCEEECCCCCCCCHHH
REIDHFLQSGELLPPPRIDEIRQVMNEHLEDHYAFYGEFTGVRTARKHIGWYTRGLSGAN
HHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
GFRHRMNTLETTREQLAAVNEFFDAQQALSDRLVYVDDEDGDRGEPDDSNQLAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
PVLGSHVLRNNLFVAPMAGVTDRPFRQLCKRLGAGYAVSEMVASNAQLWKSEKTMRRAN
CCCHHHHHHCCEEEEECCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHC
HAGEVEPIAVQIAGADPAMMAEAARHNVANGAQIIDINMGCPAKKVCNVAAGSALLQNEP
CCCCCCEEEEEEECCCHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHCHHHHCCCH
LVQRIVEAVVGAVGAGADAVPVTLKIRTGWDRDHKNALRIATLAEAAGISMLTVHGRTRA
HHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCHHHHHHHHHHCCCEEEEECCCCHH
DLYRGDAEYDTIAAVKAAVRIPVVANGDITSPRKAKAVLDATRADALMIGRAAQGRPWLF
HHHCCCCCHHHHHHHHHHHEEEEEECCCCCCCHHHHHHHHHHHHCCEEECCCCCCCCHHH
REIDHFLQSGELLPPPRIDEIRQVMNEHLEDHYAFYGEFTGVRTARKHIGWYTRGLSGAN
HHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
GFRHRMNTLETTREQLAAVNEFFDAQQALSDRLVYVDDEDGDRGEPDDSNQLAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12534463 [H]