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The map label for this gene is pckG

Identifier: 126452752

GI number: 126452752

Start: 1431841

End: 1433706

Strand: Direct

Name: pckG

Synonym: BURPS1106A_1475

Alternate gene names: 126452752

Gene position: 1431841-1433706 (Clockwise)

Preceding gene: 126451526

Following gene: 126453413

Centisome position: 35.9

GC content: 68.81

Gene sequence:

>1866_bases
ATGACCCGAAGCAACGTGGTCGCGGCGACGCGCACCGTTCCGATCGACGTTCCCGAATACGTGAAGCACCGCGGCCTGAT
CGACTGGGTCGCGCGTATCGCGGAGTTGACCGAGCCGGATCGCGTTGTCTGGTGCGACGGTTCGCAGCAGGAGTACGACC
GCCTCTGCGACGCGATGGTCGAACAGCGCACGATGGTGCGCCTCAATCCGGCGAAGCGGCCGAACTCGTTTCTCGCGCTG
TCCGATCCGTCCGACGTCGCGCGTGTCGAAGACCGCACGTTCATCTGCAGCGAGCATCGCGACGACGCGGGCCCGACCAA
TCACTGGGTCGCGCCCGCCGAAATGCGCGCGACGCTCAACGGCCTGTTTCGCGGCGCGATGCGCGGCCGCACGCTGTACG
TCGTGCCGTTCTCGATGGGCCCGCTCGGCTCGCCGATCGCGCACATCGGCGTCGAGCTCTCGGATAGCCCCTACGTCGTC
GTCAACATGCGGATCATGACGCGCATGGGGCGCGCGGTGCTCGACGCGCTCGGCGAGCGCGGCGAGTACGTGCCGTGCGT
GCACAGCGTCGGGCGCCCGCTCGCGGCGGGCGAGCAGGATGTGCCGTGGCCGTGCAATCCGACCAAGTACATCGTGCATT
TTCCCGAGTCGCGTGAGATCTGGAGCTTCGGCTCGGGCTACGGCGGCAACGCGCTCCTCGGCAAGAAGTGCTTCGCGCTG
CGGATCGCGTCGACGATGGGGCGCGACGAAGGCTGGCTCGCCGAGCACATGCTGATCCTCGGCGTGACCTCGCCCGAGGG
CCGCAAGTATCACATCGCCGCGGCGTTTCCTTCCGCGTGCGGCAAGACCAACTTCGCGATGCTGATCCCGCCGAAGGGCT
TCGAGGGCTGGCGCGTGACGACGATCGGCGACGACATCGCGTGGCTCAAGCCGGGCCGCGACGGGCGGCTGTATGCGATC
AACCCGGAGGCGGGCTATTTTGGCGTCGCGCCGGGCACGGGCGAGAAGACCAATCCGAACGCGCTCGCGACGCTCAGGGA
GAACGTGATCTTCACGAACGTCGCGCTCACGGAGGACGGCGACGTCTGGTGGGAAGGCCTCACCGACACGCCGCCCGCGC
GGCTCACCGATTGGCAGGGCAACGCATGGACGCCCGAGATCGGCCGCGAGACGGGCCGCAAGGCCGCGCATCCGAACTCG
CGCTTCACGGCGCCCGCGTCGCAGTGCCCGTCGATCGACGACGATTGGGAGAACCCGGGCGGCGTGCCGATCGACGCGTT
CATCTTCGGCGGCCGCCGCTCGACGACGGTGCCGCTCGTCACCGAGGCGCGCGACTGGATCGAAGGCGTGTACATGGCGG
CGACGATGGGCTCGGAGACGACGGCCGCGGCGGCGGGGCAGCAGGGCATCGTGCGGCGCGATCCGTTCGCGATGCTGCCG
TTCTGCGGCTACAACATGAGCGACTATTTCTCGCACTGGCTCGCGCTCGGCGAGAAGCTCGCGGCGGCGGGCGCGACGTT
GCCGAAGATCTACTGCGTGAACTGGTTCCGCAAGGACGCGGACGGCCGCTTCGCGTGGCCCGGCTTCGGCGAGAACATGC
GCGTGCTGAAGTGGATGCTCGACCGGATCGACGGCCGCGGCGAGGGCGTCGAGCACGCGTTCGGCGTGACGCCGCGCTAC
GAGGATCTGCATTGGGCGGGGCTCGCGTTCTCGCCCGCGCAGTATGCGCAGGTCACGTCGATGAATCCGGACGAATGGCG
CGCCGAGCTCGCGCTGCACGCGGAGCTGTTCGACAAGCTGAGCGCGCGGCTGCCGGATGCGCTCGCCGAAACGAAGGCGA
GGATCGAGAAAAGGCTCGGCGGCTGA

Upstream 100 bases:

>100_bases
GCGTCGCCCCCAGCCATCGCGTAGCGCCGCTACGCCCGCCGCGCGCGTCATCGCGCGGCGCACGCACGCAATTCTGGTTC
TACAACACGGAGACACTCTC

Downstream 100 bases:

>100_bases
GCAACGCGGCAAATCGCGAGCACGCCGCACGGCGGCGACGCGGCCGGGACGGCGCGCGCCGGAGCCGCGCGCGAGATATG
CCGCTTGCGCCGCCGTGCGC

Product: phosphoenolpyruvate carboxykinase

Products: NA

Alternate protein names: PEP carboxykinase; PEPCK; Phosphoenolpyruvate carboxylase

Number of amino acids: Translated: 621; Mature: 620

Protein sequence:

>621_residues
MTRSNVVAATRTVPIDVPEYVKHRGLIDWVARIAELTEPDRVVWCDGSQQEYDRLCDAMVEQRTMVRLNPAKRPNSFLAL
SDPSDVARVEDRTFICSEHRDDAGPTNHWVAPAEMRATLNGLFRGAMRGRTLYVVPFSMGPLGSPIAHIGVELSDSPYVV
VNMRIMTRMGRAVLDALGERGEYVPCVHSVGRPLAAGEQDVPWPCNPTKYIVHFPESREIWSFGSGYGGNALLGKKCFAL
RIASTMGRDEGWLAEHMLILGVTSPEGRKYHIAAAFPSACGKTNFAMLIPPKGFEGWRVTTIGDDIAWLKPGRDGRLYAI
NPEAGYFGVAPGTGEKTNPNALATLRENVIFTNVALTEDGDVWWEGLTDTPPARLTDWQGNAWTPEIGRETGRKAAHPNS
RFTAPASQCPSIDDDWENPGGVPIDAFIFGGRRSTTVPLVTEARDWIEGVYMAATMGSETTAAAAGQQGIVRRDPFAMLP
FCGYNMSDYFSHWLALGEKLAAAGATLPKIYCVNWFRKDADGRFAWPGFGENMRVLKWMLDRIDGRGEGVEHAFGVTPRY
EDLHWAGLAFSPAQYAQVTSMNPDEWRAELALHAELFDKLSARLPDALAETKARIEKRLGG

Sequences:

>Translated_621_residues
MTRSNVVAATRTVPIDVPEYVKHRGLIDWVARIAELTEPDRVVWCDGSQQEYDRLCDAMVEQRTMVRLNPAKRPNSFLAL
SDPSDVARVEDRTFICSEHRDDAGPTNHWVAPAEMRATLNGLFRGAMRGRTLYVVPFSMGPLGSPIAHIGVELSDSPYVV
VNMRIMTRMGRAVLDALGERGEYVPCVHSVGRPLAAGEQDVPWPCNPTKYIVHFPESREIWSFGSGYGGNALLGKKCFAL
RIASTMGRDEGWLAEHMLILGVTSPEGRKYHIAAAFPSACGKTNFAMLIPPKGFEGWRVTTIGDDIAWLKPGRDGRLYAI
NPEAGYFGVAPGTGEKTNPNALATLRENVIFTNVALTEDGDVWWEGLTDTPPARLTDWQGNAWTPEIGRETGRKAAHPNS
RFTAPASQCPSIDDDWENPGGVPIDAFIFGGRRSTTVPLVTEARDWIEGVYMAATMGSETTAAAAGQQGIVRRDPFAMLP
FCGYNMSDYFSHWLALGEKLAAAGATLPKIYCVNWFRKDADGRFAWPGFGENMRVLKWMLDRIDGRGEGVEHAFGVTPRY
EDLHWAGLAFSPAQYAQVTSMNPDEWRAELALHAELFDKLSARLPDALAETKARIEKRLGG
>Mature_620_residues
TRSNVVAATRTVPIDVPEYVKHRGLIDWVARIAELTEPDRVVWCDGSQQEYDRLCDAMVEQRTMVRLNPAKRPNSFLALS
DPSDVARVEDRTFICSEHRDDAGPTNHWVAPAEMRATLNGLFRGAMRGRTLYVVPFSMGPLGSPIAHIGVELSDSPYVVV
NMRIMTRMGRAVLDALGERGEYVPCVHSVGRPLAAGEQDVPWPCNPTKYIVHFPESREIWSFGSGYGGNALLGKKCFALR
IASTMGRDEGWLAEHMLILGVTSPEGRKYHIAAAFPSACGKTNFAMLIPPKGFEGWRVTTIGDDIAWLKPGRDGRLYAIN
PEAGYFGVAPGTGEKTNPNALATLRENVIFTNVALTEDGDVWWEGLTDTPPARLTDWQGNAWTPEIGRETGRKAAHPNSR
FTAPASQCPSIDDDWENPGGVPIDAFIFGGRRSTTVPLVTEARDWIEGVYMAATMGSETTAAAAGQQGIVRRDPFAMLPF
CGYNMSDYFSHWLALGEKLAAAGATLPKIYCVNWFRKDADGRFAWPGFGENMRVLKWMLDRIDGRGEGVEHAFGVTPRYE
DLHWAGLAFSPAQYAQVTSMNPDEWRAELALHAELFDKLSARLPDALAETKARIEKRLGG

Specific function: Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle

COG id: COG1274

COG function: function code C; Phosphoenolpyruvate carboxykinase (GTP)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoenolpyruvate carboxykinase [GTP] family

Homologues:

Organism=Homo sapiens, GI187281517, Length=606, Percent_Identity=50.8250825082508, Blast_Score=593, Evalue=1e-169,
Organism=Homo sapiens, GI66346721, Length=610, Percent_Identity=50.327868852459, Blast_Score=573, Evalue=1e-163,
Organism=Homo sapiens, GI66346723, Length=387, Percent_Identity=52.9715762273902, Blast_Score=391, Evalue=1e-108,
Organism=Caenorhabditis elegans, GI71989645, Length=607, Percent_Identity=48.9291598023064, Blast_Score=563, Evalue=1e-161,
Organism=Caenorhabditis elegans, GI71989658, Length=607, Percent_Identity=48.9291598023064, Blast_Score=562, Evalue=1e-160,
Organism=Caenorhabditis elegans, GI17555492, Length=617, Percent_Identity=49.2706645056726, Blast_Score=558, Evalue=1e-159,
Organism=Caenorhabditis elegans, GI71989653, Length=597, Percent_Identity=49.0787269681742, Blast_Score=555, Evalue=1e-158,
Organism=Caenorhabditis elegans, GI32564411, Length=558, Percent_Identity=52.5089605734767, Blast_Score=548, Evalue=1e-156,
Organism=Caenorhabditis elegans, GI32564407, Length=529, Percent_Identity=51.7958412098299, Blast_Score=509, Evalue=1e-144,
Organism=Caenorhabditis elegans, GI71989649, Length=482, Percent_Identity=53.1120331950208, Blast_Score=497, Evalue=1e-141,
Organism=Caenorhabditis elegans, GI32564409, Length=375, Percent_Identity=49.0666666666667, Blast_Score=324, Evalue=8e-89,
Organism=Caenorhabditis elegans, GI17540956, Length=532, Percent_Identity=24.6240601503759, Blast_Score=171, Evalue=1e-42,
Organism=Drosophila melanogaster, GI24655085, Length=597, Percent_Identity=49.5812395309883, Blast_Score=582, Evalue=1e-166,
Organism=Drosophila melanogaster, GI24655082, Length=607, Percent_Identity=48.1054365733114, Blast_Score=559, Evalue=1e-159,
Organism=Drosophila melanogaster, GI161077226, Length=576, Percent_Identity=48.7847222222222, Blast_Score=537, Evalue=1e-153,
Organism=Drosophila melanogaster, GI24655088, Length=509, Percent_Identity=50.8840864440079, Blast_Score=516, Evalue=1e-146,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PCKG_BURMA (Q62FI7)

Other databases:

- EMBL:   CP000010
- RefSeq:   YP_104540.1
- ProteinModelPortal:   Q62FI7
- SMR:   Q62FI7
- GeneID:   3089419
- GenomeReviews:   CP000010_GR
- KEGG:   bma:BMA3042
- TIGR:   BMA3042
- HOGENOM:   HBG484437
- OMA:   CGYHMGD
- ProtClustDB:   PRK04210
- BioCyc:   BMAL243160:BMA_3042-MONOMER
- BRENDA:   4.1.1.32
- GO:   GO:0005737
- GO:   GO:0006094
- HAMAP:   MF_00452
- InterPro:   IPR018091
- InterPro:   IPR013035
- InterPro:   IPR008209
- InterPro:   IPR008210
- Gene3D:   G3DSA:3.90.228.20
- Gene3D:   G3DSA:3.40.449.10
- PANTHER:   PTHR11561
- PIRSF:   PIRSF001348

Pfam domain/function: PF00821 PEPCK; SSF68923 PEP_carboxykinase_N

EC number: =4.1.1.32

Molecular weight: Translated: 68534; Mature: 68403

Theoretical pI: Translated: 6.08; Mature: 6.08

Prosite motif: PS00505 PEPCK_GTP

Important sites: ACT_SITE 280-280 BINDING 88-88 BINDING 229-229 BINDING 236-236 BINDING 278-278 BINDING 401-401 BINDING 432-432

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRSNVVAATRTVPIDVPEYVKHRGLIDWVARIAELTEPDRVVWCDGSQQEYDRLCDAMV
CCCCCEEEEEEEECCCCHHHHHHCCHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHH
EQRTMVRLNPAKRPNSFLALSDPSDVARVEDRTFICSEHRDDAGPTNHWVAPAEMRATLN
HCCCEEEECCCCCCCCEEEECCCCHHHHHCCCEEEECCCCCCCCCCCCCCCHHHHHHHHH
GLFRGAMRGRTLYVVPFSMGPLGSPIAHIGVELSDSPYVVVNMRIMTRMGRAVLDALGER
HHHHHHHCCCEEEEEEECCCCCCCCHHHEEEEECCCCEEEEEEHHHHHHHHHHHHHHHCC
GEYVPCVHSVGRPLAAGEQDVPWPCNPTKYIVHFPESREIWSFGSGYGGNALLGKKCFAL
CCEEHHHHHCCCCCCCCCCCCCCCCCCCEEEEECCCCCCEECCCCCCCCCCHHCCHHHHH
RIASTMGRDEGWLAEHMLILGVTSPEGRKYHIAAAFPSACGKTNFAMLIPPKGFEGWRVT
HHHHHCCCCCCHHEEEEEEEEECCCCCCEEEEEEECCCCCCCCCEEEEECCCCCCCCEEE
TIGDDIAWLKPGRDGRLYAINPEAGYFGVAPGTGEKTNPNALATLRENVIFTNVALTEDG
EECCCEEEECCCCCCEEEEECCCCCEEECCCCCCCCCCCHHHHHHHCCEEEEEEEEECCC
DVWWEGLTDTPPARLTDWQGNAWTPEIGRETGRKAAHPNSRFTAPASQCPSIDDDWENPG
CEEECCCCCCCCCCEECCCCCCCCCHHHHHCCCCCCCCCCCCCCCHHHCCCCCCCCCCCC
GVPIDAFIFGGRRSTTVPLVTEARDWIEGVYMAATMGSETTAAAAGQQGIVRRDPFAMLP
CCEEEEEEECCCCCCEEEEEHHHHHHHHHEEEEEECCCCHHHHHCCCCCCEECCCCEECC
FCGYNMSDYFSHWLALGEKLAAAGATLPKIYCVNWFRKDADGRFAWPGFGENMRVLKWML
CCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEHHHHCCCCCCEECCCCCCCHHHHHHHH
DRIDGRGEGVEHAFGVTPRYEDLHWAGLAFSPAQYAQVTSMNPDEWRAELALHAELFDKL
HHCCCCCCCCHHHCCCCCCCCCCEEECEEECCHHHHHCCCCCHHHHHHHHHHHHHHHHHH
SARLPDALAETKARIEKRLGG
HHHCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TRSNVVAATRTVPIDVPEYVKHRGLIDWVARIAELTEPDRVVWCDGSQQEYDRLCDAMV
CCCCEEEEEEEECCCCHHHHHHCCHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHH
EQRTMVRLNPAKRPNSFLALSDPSDVARVEDRTFICSEHRDDAGPTNHWVAPAEMRATLN
HCCCEEEECCCCCCCCEEEECCCCHHHHHCCCEEEECCCCCCCCCCCCCCCHHHHHHHHH
GLFRGAMRGRTLYVVPFSMGPLGSPIAHIGVELSDSPYVVVNMRIMTRMGRAVLDALGER
HHHHHHHCCCEEEEEEECCCCCCCCHHHEEEEECCCCEEEEEEHHHHHHHHHHHHHHHCC
GEYVPCVHSVGRPLAAGEQDVPWPCNPTKYIVHFPESREIWSFGSGYGGNALLGKKCFAL
CCEEHHHHHCCCCCCCCCCCCCCCCCCCEEEEECCCCCCEECCCCCCCCCCHHCCHHHHH
RIASTMGRDEGWLAEHMLILGVTSPEGRKYHIAAAFPSACGKTNFAMLIPPKGFEGWRVT
HHHHHCCCCCCHHEEEEEEEEECCCCCCEEEEEEECCCCCCCCCEEEEECCCCCCCCEEE
TIGDDIAWLKPGRDGRLYAINPEAGYFGVAPGTGEKTNPNALATLRENVIFTNVALTEDG
EECCCEEEECCCCCCEEEEECCCCCEEECCCCCCCCCCCHHHHHHHCCEEEEEEEEECCC
DVWWEGLTDTPPARLTDWQGNAWTPEIGRETGRKAAHPNSRFTAPASQCPSIDDDWENPG
CEEECCCCCCCCCCEECCCCCCCCCHHHHHCCCCCCCCCCCCCCCHHHCCCCCCCCCCCC
GVPIDAFIFGGRRSTTVPLVTEARDWIEGVYMAATMGSETTAAAAGQQGIVRRDPFAMLP
CCEEEEEEECCCCCCEEEEEHHHHHHHHHEEEEEECCCCHHHHHCCCCCCEECCCCEECC
FCGYNMSDYFSHWLALGEKLAAAGATLPKIYCVNWFRKDADGRFAWPGFGENMRVLKWML
CCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEHHHHCCCCCCEECCCCCCCHHHHHHHH
DRIDGRGEGVEHAFGVTPRYEDLHWAGLAFSPAQYAQVTSMNPDEWRAELALHAELFDKL
HHCCCCCCCCHHHCCCCCCCCCCEEECEEECCHHHHHCCCCCHHHHHHHHHHHHHHHHHH
SARLPDALAETKARIEKRLGG
HHHCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA