Click here to switch to the map view.

The map label for this gene is pdhB [H]

Identifier: 126452707

GI number: 126452707

Start: 2626342

End: 2627988

Strand: Reverse

Name: pdhB [H]

Synonym: BURPS1106A_2666

Alternate gene names: 126452707

Gene position: 2627988-2626342 (Counterclockwise)

Preceding gene: 126454194

Following gene: 126452790

Centisome position: 65.89

GC content: 69.52

Gene sequence:

>1647_bases
ATGAGTCAAGCGATCGAAGTCAAGGTGCCGGATATCGGCGATTACAAGGACGTGCCCGTCATCGAAGTGCTCGTGAAGCC
GGGCGATGCGGTCGAGCCCGAGCAGTCGCTCGTCACGCTCGAATCGGACAAGGCGACGATGGACGTGCCGAGCCCGTCGG
CGGGCACGGTCAAGGAAGTGAAGGTGAAGGTCGGCGACGCGGTGTCGCAAGGCTCGCTGATCGTGCTGCTCGACGGCGCG
CAGGCGGCGGCCCAGCCCGCGCAGGCGAACGGCGCCGCGACGAGCGCCGCGCAGCCGGCGGCGGCGCCCGCTGCCGCGCC
TGCGCCGGCGGCGGCCGCGGGCGGCGGCACGGTCGACGTGAAGGTGCCGGACATCGGCGACTACAAGGACGTGCCCGTCA
TCGAGATCGCCGTGAAGATCGGCGACACGGTCGAGAAGGAGCAGTCGCTCGTCACGCTCGAATCGGACAAGGCGACGATG
GACGTGCCGAGCCCGGCCGCGGGCGTCGTCAAGGACATCAAGGTGAAGGTCGGCGATGCGGTGTCGGAAGGTTCGCTGAT
CGTCGTGCTCGAAGCATCGGGCGGCGCCGCCGCGAGCGCGCCGCAGGCGGCCGCGCCCGCCCCCGCGCCGGCGCCCGCGC
CCGCGCCCGCGCCCGCGCCGCAGGCCGCACCCGCGGCTGCGCCGGCCCCCGCGCAGGCACCGGCACCCGCCGCGAGCGGC
GAGTACCGCGCGAGCCACGCGTCGCCGTCGGTGCGCAAGTTCGCGCGCGAGCTCGGCGTCGACGTGTCGCGCGTCACGGG
CACGGGGCCGAAGAGCCGCATCACGAAGGACGACGTCACCGCGTTCGTGAAGGGCGTGATGACGGGACAGCGCGCGGCGC
CCGGCGCCGCGGCCGCGCCCGCGGGCGGCGGCGAGCTGAACCTGCTGCCGTGGCCGAAGGTCGACTTCTCGAAGTTCGGC
CCGTTCGAGGCGAAGCCGCTGTCGCGCATCAAGAAGATCTCGGGCGCGAACCTGCATCGCAACTGGGTGATGATCCCGCA
CGTCACGAACAACGACGAGGCGGACATCACCGAGCTCGAAGCGCTGCGCGTGCAACTGAACAAGGAGCACGAGAAGGCGG
GCGTGAAGTTCACGATGCTCGCGTTCGTGATCAAGGCGGTCGTCGCCGCGCTGAAGAAGTTCCCGACCTTCAACGCGAGC
CTCGATGGCGACAACCTCGTGTTCAAGCAGTACTACCACATCGGTTTCGCCGCCGACACGCCGAACGGCCTCGTCGTGCC
GGTGATCCGCGACGCGGACAAGAAGGGGCTCGTCGACATCGCGAAGGAAATGGCCGAGCTGTCGAAGGCCGCGCGCGAAG
GCAAGCTCAAGCCGGACCAGATGCAGGGCGGCTGCTTCTCGATCTCGTCGCTCGGCGGGATCGGCGGCACGCACTTCACG
CCGATCATCAATGCGCCGGAAGTGGCGATCCTCGGGCTGTCGCGCGGCCAGATGAAGCCGGTGTGGGACGGCAAGCAGTT
TGTGCCGCGCCTCACGCTGCCGCTGTCGCTGTCGTATGACCATCGCGTGATCGATGGCGCGGAAGCCGCGCGGTTCAATG
CGTATCTCGGCGCGTTGCTTGCCGATTTCCGTCGCATCATTCTTTGA

Upstream 100 bases:

>100_bases
CGCTGTCGACGATTTCGACGAACGAAGCGGAGGCGGGTGGCGCGGCTGCGCCTTGCATGGGATCGGAGCGGCCGCTCCGA
TCGACAGGAGACTGAAACAG

Downstream 100 bases:

>100_bases
TGAGCGTGGGAGTGGCTCGCGGGGGCGTGGGTTTTTTGTCGGTGATCCGCGCTTCGTCGTTGAACCTGTTGCGCTAACGG
TCTATCAGCGTCGCCCCTGC

Product: dihydrolipoamide acetyltransferase

Products: NA

Alternate protein names: Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; E2 [H]

Number of amino acids: Translated: 548; Mature: 547

Protein sequence:

>548_residues
MSQAIEVKVPDIGDYKDVPVIEVLVKPGDAVEPEQSLVTLESDKATMDVPSPSAGTVKEVKVKVGDAVSQGSLIVLLDGA
QAAAQPAQANGAATSAAQPAAAPAAAPAPAAAAGGGTVDVKVPDIGDYKDVPVIEIAVKIGDTVEKEQSLVTLESDKATM
DVPSPAAGVVKDIKVKVGDAVSEGSLIVVLEASGGAAASAPQAAAPAPAPAPAPAPAPAPQAAPAAAPAPAQAPAPAASG
EYRASHASPSVRKFARELGVDVSRVTGTGPKSRITKDDVTAFVKGVMTGQRAAPGAAAAPAGGGELNLLPWPKVDFSKFG
PFEAKPLSRIKKISGANLHRNWVMIPHVTNNDEADITELEALRVQLNKEHEKAGVKFTMLAFVIKAVVAALKKFPTFNAS
LDGDNLVFKQYYHIGFAADTPNGLVVPVIRDADKKGLVDIAKEMAELSKAAREGKLKPDQMQGGCFSISSLGGIGGTHFT
PIINAPEVAILGLSRGQMKPVWDGKQFVPRLTLPLSLSYDHRVIDGAEAARFNAYLGALLADFRRIIL

Sequences:

>Translated_548_residues
MSQAIEVKVPDIGDYKDVPVIEVLVKPGDAVEPEQSLVTLESDKATMDVPSPSAGTVKEVKVKVGDAVSQGSLIVLLDGA
QAAAQPAQANGAATSAAQPAAAPAAAPAPAAAAGGGTVDVKVPDIGDYKDVPVIEIAVKIGDTVEKEQSLVTLESDKATM
DVPSPAAGVVKDIKVKVGDAVSEGSLIVVLEASGGAAASAPQAAAPAPAPAPAPAPAPAPQAAPAAAPAPAQAPAPAASG
EYRASHASPSVRKFARELGVDVSRVTGTGPKSRITKDDVTAFVKGVMTGQRAAPGAAAAPAGGGELNLLPWPKVDFSKFG
PFEAKPLSRIKKISGANLHRNWVMIPHVTNNDEADITELEALRVQLNKEHEKAGVKFTMLAFVIKAVVAALKKFPTFNAS
LDGDNLVFKQYYHIGFAADTPNGLVVPVIRDADKKGLVDIAKEMAELSKAAREGKLKPDQMQGGCFSISSLGGIGGTHFT
PIINAPEVAILGLSRGQMKPVWDGKQFVPRLTLPLSLSYDHRVIDGAEAARFNAYLGALLADFRRIIL
>Mature_547_residues
SQAIEVKVPDIGDYKDVPVIEVLVKPGDAVEPEQSLVTLESDKATMDVPSPSAGTVKEVKVKVGDAVSQGSLIVLLDGAQ
AAAQPAQANGAATSAAQPAAAPAAAPAPAAAAGGGTVDVKVPDIGDYKDVPVIEIAVKIGDTVEKEQSLVTLESDKATMD
VPSPAAGVVKDIKVKVGDAVSEGSLIVVLEASGGAAASAPQAAAPAPAPAPAPAPAPAPQAAPAAAPAPAQAPAPAASGE
YRASHASPSVRKFARELGVDVSRVTGTGPKSRITKDDVTAFVKGVMTGQRAAPGAAAAPAGGGELNLLPWPKVDFSKFGP
FEAKPLSRIKKISGANLHRNWVMIPHVTNNDEADITELEALRVQLNKEHEKAGVKFTMLAFVIKAVVAALKKFPTFNASL
DGDNLVFKQYYHIGFAADTPNGLVVPVIRDADKKGLVDIAKEMAELSKAAREGKLKPDQMQGGCFSISSLGGIGGTHFTP
IINAPEVAILGLSRGQMKPVWDGKQFVPRLTLPLSLSYDHRVIDGAEAARFNAYLGALLADFRRIIL

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 lipoyl-binding domains [H]

Homologues:

Organism=Homo sapiens, GI110671329, Length=461, Percent_Identity=29.2841648590022, Blast_Score=170, Evalue=3e-42,
Organism=Homo sapiens, GI31711992, Length=424, Percent_Identity=31.8396226415094, Blast_Score=159, Evalue=7e-39,
Organism=Homo sapiens, GI203098816, Length=484, Percent_Identity=27.6859504132231, Blast_Score=139, Evalue=8e-33,
Organism=Homo sapiens, GI203098753, Length=440, Percent_Identity=28.4090909090909, Blast_Score=136, Evalue=5e-32,
Organism=Homo sapiens, GI19923748, Length=227, Percent_Identity=34.3612334801762, Blast_Score=122, Evalue=7e-28,
Organism=Homo sapiens, GI260898739, Length=153, Percent_Identity=37.2549019607843, Blast_Score=96, Evalue=6e-20,
Organism=Escherichia coli, GI1786305, Length=547, Percent_Identity=52.8336380255942, Blast_Score=510, Evalue=1e-146,
Organism=Escherichia coli, GI1786946, Length=434, Percent_Identity=31.3364055299539, Blast_Score=186, Evalue=2e-48,
Organism=Caenorhabditis elegans, GI17537937, Length=444, Percent_Identity=28.3783783783784, Blast_Score=170, Evalue=2e-42,
Organism=Caenorhabditis elegans, GI17560088, Length=443, Percent_Identity=31.3769751693002, Blast_Score=152, Evalue=6e-37,
Organism=Caenorhabditis elegans, GI25146366, Length=425, Percent_Identity=31.2941176470588, Blast_Score=145, Evalue=6e-35,
Organism=Caenorhabditis elegans, GI17538894, Length=309, Percent_Identity=33.0097087378641, Blast_Score=119, Evalue=3e-27,
Organism=Saccharomyces cerevisiae, GI6320352, Length=419, Percent_Identity=29.8329355608592, Blast_Score=172, Evalue=2e-43,
Organism=Saccharomyces cerevisiae, GI6324258, Length=434, Percent_Identity=29.0322580645161, Blast_Score=131, Evalue=3e-31,
Organism=Drosophila melanogaster, GI18859875, Length=431, Percent_Identity=32.7146171693735, Blast_Score=184, Evalue=2e-46,
Organism=Drosophila melanogaster, GI24582497, Length=232, Percent_Identity=31.4655172413793, Blast_Score=124, Evalue=2e-28,
Organism=Drosophila melanogaster, GI20129315, Length=232, Percent_Identity=31.4655172413793, Blast_Score=124, Evalue=2e-28,
Organism=Drosophila melanogaster, GI24645909, Length=207, Percent_Identity=35.2657004830918, Blast_Score=120, Evalue=2e-27,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 912 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR006256
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.12 [H]

Molecular weight: Translated: 56434; Mature: 56303

Theoretical pI: Translated: 6.43; Mature: 6.43

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQAIEVKVPDIGDYKDVPVIEVLVKPGDAVEPEQSLVTLESDKATMDVPSPSAGTVKEV
CCCEEEEECCCCCCCCCCCEEEEEECCCCCCCCCCCEEEEECCCCEEECCCCCCCCCEEE
KVKVGDAVSQGSLIVLLDGAQAAAQPAQANGAATSAAQPAAAPAAAPAPAAAAGGGTVDV
EEEECCCCCCCCEEEEECCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEE
KVPDIGDYKDVPVIEIAVKIGDTVEKEQSLVTLESDKATMDVPSPAAGVVKDIKVKVGDA
ECCCCCCCCCCCEEEEEEECCCCHHCCCCEEEEECCCCEECCCCCHHHHHHHEEEEECCC
VSEGSLIVVLEASGGAAASAPQAAAPAPAPAPAPAPAPAPQAAPAAAPAPAQAPAPAASG
CCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
EYRASHASPSVRKFARELGVDVSRVTGTGPKSRITKDDVTAFVKGVMTGQRAAPGAAAAP
CCCCCCCCHHHHHHHHHHCCEEEEECCCCCHHCCCHHHHHHHHHHHHCCCCCCCCCCCCC
AGGGELNLLPWPKVDFSKFGPFEAKPLSRIKKISGANLHRNWVMIPHVTNNDEADITELE
CCCCEEEECCCCCCCHHHCCCCCCCHHHHHHHHCCCCEECCEEEEEEECCCCCCCHHHHH
ALRVQLNKEHEKAGVKFTMLAFVIKAVVAALKKFPTFNASLDGDNLVFKQYYHIGFAADT
HHHHHHCCHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEEEEEEECC
PNGLVVPVIRDADKKGLVDIAKEMAELSKAAREGKLKPDQMQGGCFSISSLGGIGGTHFT
CCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHCCCEEEECCCCCCCCCCCC
PIINAPEVAILGLSRGQMKPVWDGKQFVPRLTLPLSLSYDHRVIDGAEAARFNAYLGALL
EECCCCCEEEEECCCCCCCCCCCCHHHCCEEEEEEEECCCCEEECCCHHHHHHHHHHHHH
ADFRRIIL
HHHHHHHC
>Mature Secondary Structure 
SQAIEVKVPDIGDYKDVPVIEVLVKPGDAVEPEQSLVTLESDKATMDVPSPSAGTVKEV
CCEEEEECCCCCCCCCCCEEEEEECCCCCCCCCCCEEEEECCCCEEECCCCCCCCCEEE
KVKVGDAVSQGSLIVLLDGAQAAAQPAQANGAATSAAQPAAAPAAAPAPAAAAGGGTVDV
EEEECCCCCCCCEEEEECCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEE
KVPDIGDYKDVPVIEIAVKIGDTVEKEQSLVTLESDKATMDVPSPAAGVVKDIKVKVGDA
ECCCCCCCCCCCEEEEEEECCCCHHCCCCEEEEECCCCEECCCCCHHHHHHHEEEEECCC
VSEGSLIVVLEASGGAAASAPQAAAPAPAPAPAPAPAPAPQAAPAAAPAPAQAPAPAASG
CCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
EYRASHASPSVRKFARELGVDVSRVTGTGPKSRITKDDVTAFVKGVMTGQRAAPGAAAAP
CCCCCCCCHHHHHHHHHHCCEEEEECCCCCHHCCCHHHHHHHHHHHHCCCCCCCCCCCCC
AGGGELNLLPWPKVDFSKFGPFEAKPLSRIKKISGANLHRNWVMIPHVTNNDEADITELE
CCCCEEEECCCCCCCHHHCCCCCCCHHHHHHHHCCCCEECCEEEEEEECCCCCCCHHHHH
ALRVQLNKEHEKAGVKFTMLAFVIKAVVAALKKFPTFNASLDGDNLVFKQYYHIGFAADT
HHHHHHCCHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEEEEEEECC
PNGLVVPVIRDADKKGLVDIAKEMAELSKAAREGKLKPDQMQGGCFSISSLGGIGGTHFT
CCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHCCCEEEECCCCCCCCCCCC
PIINAPEVAILGLSRGQMKPVWDGKQFVPRLTLPLSLSYDHRVIDGAEAARFNAYLGALL
EECCCCCEEEEECCCCCCCCCCCCHHHCCEEEEEEEECCCCEEECCCHHHHHHHHHHHHH
ADFRRIIL
HHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8021225 [H]