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The map label for this gene is ampD [H]

Identifier: 126452698

GI number: 126452698

Start: 3419619

End: 3420209

Strand: Reverse

Name: ampD [H]

Synonym: BURPS1106A_3514

Alternate gene names: 126452698

Gene position: 3420209-3419619 (Counterclockwise)

Preceding gene: 126454725

Following gene: 126454560

Centisome position: 85.75

GC content: 68.36

Gene sequence:

>591_bases
ATGAGCGAGGCGGGGCGCTTCGCGGTCGGCGCCGACGGCTGGGTCGACGGCGCGCGGCGCGAGCCGTCGCCGAATTTCGA
AGCGCGGCCGGACGGCGCAGCCATCTCGCTCGTCGTGATCCACAACATCAGTCTGCCGCCCGGCGAATTCGGCGGCGACG
CGATCGTCGCGCTGTTCCAGAATCGTCTCGACTGCGATGCGCATCCGTACTACGCCGCGCATCTGCGCGGCGTGCGGGTT
TCCGCGCATTTCCTCGTCAGGCGGGACGGCGCGCTGATCCAGTTCGTGTCGTGCGACGCGCGCGCTTGGCACGCGGGCGC
GTCGAGCTTCTTCGGCCGCGAGCGCTGTAACGATTTTTCAATCGGCATCGAGCTCGAAGGCGCGGACGACGTCGCGTTCG
ACGCGCGGCAATACGCGACGCTCGCCGCGCTCGCACGCGCGCTTGCCGCGCGTTACCCGATCGATGCGTTCGCGGGCCAC
GAGCACGTTGCGCCGGGACGCAAGACCGACCCCGGTCCGCATTTCGATTGGCAACGTTTCGCGGACGATGGCGGTTTCCC
GCCTCGATACTTTCCCTACCGGAAGCACTGA

Upstream 100 bases:

>100_bases
ATGCGCCGAAGAGCGACGCCGTCGTCGCGGGCGGCGAGTATTTCTGCAGTCCGGAGCACGCGGCGCGCCATGCCGCGCAT
TCGGGCAGCCGCAGCGGGCA

Downstream 100 bases:

>100_bases
CGGCAGCGGCGGGCGCGTGGTCTTTTTGACGCGTGCCGTATTGTTTCGCCGGCCACGCCTTGTCGCGTCGGCGTATCGCG
CTTGTCAATAGATCGCCAGC

Product: N-acetyl-anhydromuranmyl-L-alanine amidase

Products: NA

Alternate protein names: N-acetylmuramoyl-L-alanine amidase [H]

Number of amino acids: Translated: 196; Mature: 195

Protein sequence:

>196_residues
MSEAGRFAVGADGWVDGARREPSPNFEARPDGAAISLVVIHNISLPPGEFGGDAIVALFQNRLDCDAHPYYAAHLRGVRV
SAHFLVRRDGALIQFVSCDARAWHAGASSFFGRERCNDFSIGIELEGADDVAFDARQYATLAALARALAARYPIDAFAGH
EHVAPGRKTDPGPHFDWQRFADDGGFPPRYFPYRKH

Sequences:

>Translated_196_residues
MSEAGRFAVGADGWVDGARREPSPNFEARPDGAAISLVVIHNISLPPGEFGGDAIVALFQNRLDCDAHPYYAAHLRGVRV
SAHFLVRRDGALIQFVSCDARAWHAGASSFFGRERCNDFSIGIELEGADDVAFDARQYATLAALARALAARYPIDAFAGH
EHVAPGRKTDPGPHFDWQRFADDGGFPPRYFPYRKH
>Mature_195_residues
SEAGRFAVGADGWVDGARREPSPNFEARPDGAAISLVVIHNISLPPGEFGGDAIVALFQNRLDCDAHPYYAAHLRGVRVS
AHFLVRRDGALIQFVSCDARAWHAGASSFFGRERCNDFSIGIELEGADDVAFDARQYATLAALARALAARYPIDAFAGHE
HVAPGRKTDPGPHFDWQRFADDGGFPPRYFPYRKH

Specific function: Involved in both cell wall peptidoglycans recycling and beta-lactamase induction. Specifically cleaves the amide bond between the lactyl group of N-acetylmuramic acid and the alpha- amino group of the L-alanine in degradation products containing an anhydr

COG id: COG3023

COG function: function code V; Negative regulator of beta-lactamase expression

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 2 family [H]

Homologues:

Organism=Escherichia coli, GI1786300, Length=172, Percent_Identity=59.8837209302326, Blast_Score=199, Evalue=1e-52,
Organism=Escherichia coli, GI1787092, Length=130, Percent_Identity=40.7692307692308, Blast_Score=75, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002502 [H]

Pfam domain/function: PF01510 Amidase_2 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 21468; Mature: 21337

Theoretical pI: Translated: 6.60; Mature: 6.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEAGRFAVGADGWVDGARREPSPNFEARPDGAAISLVVIHNISLPPGEFGGDAIVALFQ
CCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEECCCCCCCCCCHHHHHHHH
NRLDCDAHPYYAAHLRGVRVSAHFLVRRDGALIQFVSCDARAWHAGASSFFGRERCNDFS
HCCCCCCCCEEEHHHCCEEEEEEEEEECCCCEEEEEECCCCHHHCCCHHHHHHHCCCCEE
IGIELEGADDVAFDARQYATLAALARALAARYPIDAFAGHEHVAPGRKTDPGPHFDWQRF
EEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCCCCCCCCCCCHHHH
ADDGGFPPRYFPYRKH
CCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SEAGRFAVGADGWVDGARREPSPNFEARPDGAAISLVVIHNISLPPGEFGGDAIVALFQ
CCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEECCCCCCCCCCHHHHHHHH
NRLDCDAHPYYAAHLRGVRVSAHFLVRRDGALIQFVSCDARAWHAGASSFFGRERCNDFS
HCCCCCCCCEEEHHHCCEEEEEEEEEECCCCEEEEEECCCCHHHCCCHHHHHHHCCCCEE
IGIELEGADDVAFDARQYATLAALARALAARYPIDAFAGHEHVAPGRKTDPGPHFDWQRF
EEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCCCCCCCCCCCHHHH
ADDGGFPPRYFPYRKH
CCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8383940 [H]