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The map label for this gene is gloB [H]

Identifier: 126452658

GI number: 126452658

Start: 1453306

End: 1454112

Strand: Direct

Name: gloB [H]

Synonym: BURPS1106A_1498

Alternate gene names: 126452658

Gene position: 1453306-1454112 (Clockwise)

Preceding gene: 126454434

Following gene: 126451560

Centisome position: 36.44

GC content: 72.86

Gene sequence:

>807_bases
ATGAACGAGCTGGAATACGTGCCGGTGCCGGCATTCGACGACAACTACATCTGGCTCGTGTCGGACGGCCGCCAGGCGGT
CGCCGTCGATCCGGGCGAAGCCGCGCCGGTGCGCCGCCATCTCGACGCGCGAGGCTGGCGGCTGACCGCTATTTTACTCA
CGCACCACCACCGCGACCACGTCGGCGGAGTGGCCGAGCTGCTCGCGGGCGCGCGCGAAGGCGAGCCGATCGCGGTGTTC
GGCCCCGCGCACGAGGCGATCGAGCACATCACGCGCCGCGTGGCGGGCGGCGAGCGCGTGCGCATCGGCGCGCCCGCGCT
CGAGTTCGACGTGATCGACGTGCCGGGCCACACGCGCGGCCACATCGCATACTTCCAGCCGGCGGGGCCGGGCAACGCCG
CGCCGCATCTCTTTTGCGGCGACACGCTGTTCTCGTGCGGCTGCGGCCGCCTCTTCGAGGGCACGCCCGCGCAGATGCTC
GCGTCGCTCGACGCGCTCGCGGCGCTGCCGGGCGACACGCGCGTGCACTGCGCGCACGAGTACACGCTGTCGAACATCCA
GTTCGCGCTCGCATGCGAGCCGGGGAACGCGGCGCTCGCCGCGTGGCGCGACGAAGCGCGCGCGCGCCGCGCCCGCGGCG
AGCCGACGTTGCCCACGACGATCGCGCACGAGTGCGCGGTGAACCCGTTCCTGCGCGCGGACAGCGCCTCGATCCGCGCG
ACGCTCGAGGCGCGGCTGCACGAGGCGGCGCCCGACCGGCTCGCGGCGTTCACGTTGATGCGCGAGTGGAAAAACCGTTT
TCGTTGA

Upstream 100 bases:

>100_bases
GCCTGCTCCCAGCCAAGCACATAGCGCCCGGGCGGGGAGTCGGTCCAGGCGGGCCAGTCTATAATCGAACGGTCAGACAT
ATCTCGGTGATTGCGCGCCC

Downstream 100 bases:

>100_bases
GCCCGTGCAATAGTCGGCCAAGCTCAACCGGAATCTCGAGAATCGGCCCCAAATACCCGATTTAGCAGGTTTTTTCGGCC
TTTTTGTTGACGCGAAGCAC

Product: metallo-beta-lactamase family protein

Products: NA

Alternate protein names: Glyoxalase II; Glx II [H]

Number of amino acids: Translated: 268; Mature: 268

Protein sequence:

>268_residues
MNELEYVPVPAFDDNYIWLVSDGRQAVAVDPGEAAPVRRHLDARGWRLTAILLTHHHRDHVGGVAELLAGAREGEPIAVF
GPAHEAIEHITRRVAGGERVRIGAPALEFDVIDVPGHTRGHIAYFQPAGPGNAAPHLFCGDTLFSCGCGRLFEGTPAQML
ASLDALAALPGDTRVHCAHEYTLSNIQFALACEPGNAALAAWRDEARARRARGEPTLPTTIAHECAVNPFLRADSASIRA
TLEARLHEAAPDRLAAFTLMREWKNRFR

Sequences:

>Translated_268_residues
MNELEYVPVPAFDDNYIWLVSDGRQAVAVDPGEAAPVRRHLDARGWRLTAILLTHHHRDHVGGVAELLAGAREGEPIAVF
GPAHEAIEHITRRVAGGERVRIGAPALEFDVIDVPGHTRGHIAYFQPAGPGNAAPHLFCGDTLFSCGCGRLFEGTPAQML
ASLDALAALPGDTRVHCAHEYTLSNIQFALACEPGNAALAAWRDEARARRARGEPTLPTTIAHECAVNPFLRADSASIRA
TLEARLHEAAPDRLAAFTLMREWKNRFR
>Mature_268_residues
MNELEYVPVPAFDDNYIWLVSDGRQAVAVDPGEAAPVRRHLDARGWRLTAILLTHHHRDHVGGVAELLAGAREGEPIAVF
GPAHEAIEHITRRVAGGERVRIGAPALEFDVIDVPGHTRGHIAYFQPAGPGNAAPHLFCGDTLFSCGCGRLFEGTPAQML
ASLDALAALPGDTRVHCAHEYTLSNIQFALACEPGNAALAAWRDEARARRARGEPTLPTTIAHECAVNPFLRADSASIRA
TLEARLHEAAPDRLAAFTLMREWKNRFR

Specific function: Thiolesterase that catalyzes the hydrolysis of S-D- lactoyl-glutathione to form glutathione and D-lactic acid [H]

COG id: COG0491

COG function: function code R; Zn-dependent hydrolases, including glyoxylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family [H]

Homologues:

Organism=Homo sapiens, GI94538320, Length=263, Percent_Identity=37.2623574144487, Blast_Score=169, Evalue=2e-42,
Organism=Homo sapiens, GI94538322, Length=263, Percent_Identity=36.5019011406844, Blast_Score=168, Evalue=5e-42,
Organism=Homo sapiens, GI14150041, Length=233, Percent_Identity=41.6309012875536, Blast_Score=154, Evalue=7e-38,
Organism=Homo sapiens, GI116642887, Length=244, Percent_Identity=35.2459016393443, Blast_Score=145, Evalue=4e-35,
Organism=Homo sapiens, GI21703352, Length=244, Percent_Identity=35.2459016393443, Blast_Score=144, Evalue=6e-35,
Organism=Homo sapiens, GI46361987, Length=203, Percent_Identity=42.8571428571429, Blast_Score=134, Evalue=8e-32,
Organism=Escherichia coli, GI1786406, Length=262, Percent_Identity=45.8015267175573, Blast_Score=198, Evalue=2e-52,
Organism=Escherichia coli, GI1787158, Length=193, Percent_Identity=34.1968911917098, Blast_Score=70, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17536925, Length=260, Percent_Identity=34.6153846153846, Blast_Score=140, Evalue=5e-34,
Organism=Saccharomyces cerevisiae, GI6320478, Length=269, Percent_Identity=31.2267657992565, Blast_Score=100, Evalue=3e-22,
Organism=Saccharomyces cerevisiae, GI6324614, Length=239, Percent_Identity=30.1255230125523, Blast_Score=84, Evalue=2e-17,
Organism=Drosophila melanogaster, GI21356335, Length=268, Percent_Identity=36.5671641791045, Blast_Score=166, Evalue=1e-41,
Organism=Drosophila melanogaster, GI24667711, Length=268, Percent_Identity=36.5671641791045, Blast_Score=166, Evalue=1e-41,
Organism=Drosophila melanogaster, GI24667703, Length=268, Percent_Identity=36.5671641791045, Blast_Score=165, Evalue=3e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001279
- InterPro:   IPR017782 [H]

Pfam domain/function: PF00753 Lactamase_B [H]

EC number: =3.1.2.6 [H]

Molecular weight: Translated: 29131; Mature: 29131

Theoretical pI: Translated: 6.62; Mature: 6.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNELEYVPVPAFDDNYIWLVSDGRQAVAVDPGEAAPVRRHLDARGWRLTAILLTHHHRDH
CCCCCEEECCCCCCCEEEEEECCCEEEEECCCCCCHHHHHHCCCCEEEEEEEEECCCCHH
VGGVAELLAGAREGEPIAVFGPAHEAIEHITRRVAGGERVRIGAPALEFDVIDVPGHTRG
HHHHHHHHHCCCCCCCEEEECCHHHHHHHHHHHHCCCCEEEECCCCEEEEEEECCCCCCC
HIAYFQPAGPGNAAPHLFCGDTLFSCGCGRLFEGTPAQMLASLDALAALPGDTRVHCAHE
CEEEECCCCCCCCCCEEEECCHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEEE
YTLSNIQFALACEPGNAALAAWRDEARARRARGEPTLPTTIAHECAVNPFLRADSASIRA
EEECCEEEEEEECCCCCEEEHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCEECCCCHHHH
TLEARLHEAAPDRLAAFTLMREWKNRFR
HHHHHHHHCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNELEYVPVPAFDDNYIWLVSDGRQAVAVDPGEAAPVRRHLDARGWRLTAILLTHHHRDH
CCCCCEEECCCCCCCEEEEEECCCEEEEECCCCCCHHHHHHCCCCEEEEEEEEECCCCHH
VGGVAELLAGAREGEPIAVFGPAHEAIEHITRRVAGGERVRIGAPALEFDVIDVPGHTRG
HHHHHHHHHCCCCCCCEEEECCHHHHHHHHHHHHCCCCEEEECCCCEEEEEEECCCCCCC
HIAYFQPAGPGNAAPHLFCGDTLFSCGCGRLFEGTPAQMLASLDALAALPGDTRVHCAHE
CEEEECCCCCCCCCCEEEECCHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEEE
YTLSNIQFALACEPGNAALAAWRDEARARRARGEPTLPTTIAHECAVNPFLRADSASIRA
EEECCEEEEEEECCCCCEEEHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCEECCCCHHHH
TLEARLHEAAPDRLAAFTLMREWKNRFR
HHHHHHHHCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA