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The map label for this gene is phaZ

Identifier: 126452644

GI number: 126452644

Start: 1165754

End: 1167220

Strand: Reverse

Name: phaZ

Synonym: BURPS1106A_1186

Alternate gene names: NA

Gene position: 1167220-1165754 (Counterclockwise)

Preceding gene: 126454902

Following gene: 126453295

Centisome position: 29.26

GC content: 66.26

Gene sequence:

>1467_bases
ATGCTTTACCAATTTCACGAATTCCAGCGGGCGATGCTGAGCCCGCTGACCGCCTGGGCACAAGCCGCGTCCAAATCGTT
CGCGAACCCGTCGAGTCCGCTTTCGCTCGTACCGGGCGCGACGCGCCTCGCGGCGGGCTACGAACTGCTGTACCGGCTCG
GCAAGGATTACGAAAAGCCGGAGTTCGGGATTCACCAAATCGTCAAGGACGGACACAACATTCCGATCGTCGAGCAGACG
ATCATCGAAAAGCCGTTCTGCCGGCTGCTGCGCTTCAAGCGCTACGCGGACGATTCGGGCGCCGTCGGGCAACTGAAGGA
CGAGCCCGTCGTGCTCGTGTGCGCGCCGCTGTCGGGCCACCACGCCACGCTGCTGCGCGACACGGTCCGCACGCTGCTGC
AGGACCACAAGGTCTACATCACCGACTGGATCGACGCGCGGATGGTGCCCGCCGAAGTCGGCTCGTTTCATCTCGACGAC
TACGTCGGCTACATCCAGAAATTCATCCGGCATATCGGCGCGCGCAACCTGCACGTGATCTCGGTATGTCAGCCGACCGT
GCCCGTGCTCGCGGCGATCTCGCTGATGGCGAGCCGCGGCGAAGACACGCCGCTCACGATGACGATGATGGGCGGGCCGA
TCGACGCGCGCAAGAGCCCGACGTCGGTGAATTCGCTCGCGACCAACCGCTCGCACTCGTGGTTCGAGAACAACGTGATC
CACACGGTGCCGGCGAACTATCCGGGCGAGGGTCGCAAGGTCTATCCGGGCTTCCTGCAGCACACGGGCTTCGTCGCGAT
GAACCCGGAGCGCCACGCGCAATCGCACTGGGACTTCTACCAGAGCCTGCTGCGCGGCGACGAGGAAGACGCCGAGGCAC
ACCGCCGCTTCTACGACGAATACAACGCGGTGCTCGACATGGCGGCCGAATACTATCTGGACACGATCCGCATCGTGTTC
CAGGAGTTCCGCCTCGCCGAGGGCACGTGGGACATCCACGGCGAGCGCGTGAAGCCGGCCGATATCCATTCGACCGCGCT
GATGACGATCGAGGGCGAACTCGACGACATCTCGGGCAGCGGCCAGACGCACGTCGCGCACGAGCTGTGCACGGGCATCG
AACAGGCGCACCGCCGCAGCCTCACCGCCGAGAAGTGCGGCCACTACGGCATTTTCTCGGGCCGCCGCTGGCGCACGATC
ATCTATCCGCAACTGCGCGACTTCATCCGCGAGCACGCGTCGCAGCCGAAGAAGCCGACGCCGCAGGCCGGTTCCACGCC
CAACTCGCCTTCCACGCCGAGCCGATCGGCATCGGGCAGCGAAACGGCGAGCGCCGCGCCCGCGAAAGCGGCCGCGCTCA
AGCTGAGCGCGAAGCGCACCGCCGCCAAAACCCGTGCGGCGAAGCCGCCCGCCGCCAAGCGCGGCGCAATCAAGCTCGCC
GCGCCCCGCACGCGCAAGGCCGCCTGA

Upstream 100 bases:

>100_bases
ATTCGATTCCACCACCCGAATCGGCCCAATTGCTGACTCATACGGCACGCCGCGACGCCCCGCGCCGCCTTCGCGCACCG
TTCTCCCATCGGGAGCTCGC

Downstream 100 bases:

>100_bases
CGCGCGCCCGCGGTCTCGCGCGCCGCCGCGCCGCGCGCCGCTTCGCGCAACCGGGCGCGAACGGCGCGTGTTTTCTTGGA
GCGGCGCGGCGCGAAGCGGC

Product: intracellular poly[D(-)-3-hydroxybutyrate] (PHB) depolymerase

Products: NA

Alternate protein names: Intracellular PHB Depolymerase; Polyhydroxyalkanoate Depolymerase; Poly-Beta-Hydroxyalkanoate Depolymerase; PHB Depolymerase; PHB De-Polymerase Domain-Containing Protein; Poly[D(-)-3-Hydroxybutyrate] Depolymerase; Intracellular Poly(3-Hydroxybutyrate) Depolymerase; Intracellular Polyhydroxyalkanoate Depolymerase; Polyhydroxyalkanoate Depolymerase Intracellular Subfamily; Poly(3-Hydroxyalkanoate) Depolymerase; Poly(3-Hydroxybutyrate) Depolymerase; Alpha/Beta Hydrolase Fold; Alpha/Beta Hydrolase Fold Protein; Intracellular PHB Depolymerase Protein; Poly-Beta-Hydroxyalkanoate Depolymerase-Like Protein; PHB De-Polymerase Domain Protein; PHB De-Polymerase; Intracellular Poly[D(-)-3-Hydroxybutyrate] Depolymerase; Poly[D(-)-3-Hydroxybutyrate](Phb) Depolymerase; Poly-Beta-Hydroxyalkanoate/Butyrate Depolymerase; Poly-Beta-Hydroxyalkanoate Depolymerase Protein; Poly[D(-)-3-Hydroxyalkanoate] Depolymerase; Polyhydroxybutanoate Depolymerase; Poly-Beta-Hydroxybutyrate Depolymerase

Number of amino acids: Translated: 488; Mature: 488

Protein sequence:

>488_residues
MLYQFHEFQRAMLSPLTAWAQAASKSFANPSSPLSLVPGATRLAAGYELLYRLGKDYEKPEFGIHQIVKDGHNIPIVEQT
IIEKPFCRLLRFKRYADDSGAVGQLKDEPVVLVCAPLSGHHATLLRDTVRTLLQDHKVYITDWIDARMVPAEVGSFHLDD
YVGYIQKFIRHIGARNLHVISVCQPTVPVLAAISLMASRGEDTPLTMTMMGGPIDARKSPTSVNSLATNRSHSWFENNVI
HTVPANYPGEGRKVYPGFLQHTGFVAMNPERHAQSHWDFYQSLLRGDEEDAEAHRRFYDEYNAVLDMAAEYYLDTIRIVF
QEFRLAEGTWDIHGERVKPADIHSTALMTIEGELDDISGSGQTHVAHELCTGIEQAHRRSLTAEKCGHYGIFSGRRWRTI
IYPQLRDFIREHASQPKKPTPQAGSTPNSPSTPSRSASGSETASAAPAKAAALKLSAKRTAAKTRAAKPPAAKRGAIKLA
APRTRKAA

Sequences:

>Translated_488_residues
MLYQFHEFQRAMLSPLTAWAQAASKSFANPSSPLSLVPGATRLAAGYELLYRLGKDYEKPEFGIHQIVKDGHNIPIVEQT
IIEKPFCRLLRFKRYADDSGAVGQLKDEPVVLVCAPLSGHHATLLRDTVRTLLQDHKVYITDWIDARMVPAEVGSFHLDD
YVGYIQKFIRHIGARNLHVISVCQPTVPVLAAISLMASRGEDTPLTMTMMGGPIDARKSPTSVNSLATNRSHSWFENNVI
HTVPANYPGEGRKVYPGFLQHTGFVAMNPERHAQSHWDFYQSLLRGDEEDAEAHRRFYDEYNAVLDMAAEYYLDTIRIVF
QEFRLAEGTWDIHGERVKPADIHSTALMTIEGELDDISGSGQTHVAHELCTGIEQAHRRSLTAEKCGHYGIFSGRRWRTI
IYPQLRDFIREHASQPKKPTPQAGSTPNSPSTPSRSASGSETASAAPAKAAALKLSAKRTAAKTRAAKPPAAKRGAIKLA
APRTRKAA
>Mature_488_residues
MLYQFHEFQRAMLSPLTAWAQAASKSFANPSSPLSLVPGATRLAAGYELLYRLGKDYEKPEFGIHQIVKDGHNIPIVEQT
IIEKPFCRLLRFKRYADDSGAVGQLKDEPVVLVCAPLSGHHATLLRDTVRTLLQDHKVYITDWIDARMVPAEVGSFHLDD
YVGYIQKFIRHIGARNLHVISVCQPTVPVLAAISLMASRGEDTPLTMTMMGGPIDARKSPTSVNSLATNRSHSWFENNVI
HTVPANYPGEGRKVYPGFLQHTGFVAMNPERHAQSHWDFYQSLLRGDEEDAEAHRRFYDEYNAVLDMAAEYYLDTIRIVF
QEFRLAEGTWDIHGERVKPADIHSTALMTIEGELDDISGSGQTHVAHELCTGIEQAHRRSLTAEKCGHYGIFSGRRWRTI
IYPQLRDFIREHASQPKKPTPQAGSTPNSPSTPSRSASGSETASAAPAKAAALKLSAKRTAAKTRAAKPPAAKRGAIKLA
APRTRKAA

Specific function: Unknown

COG id: COG4553

COG function: function code I; Poly-beta-hydroxyalkanoate depolymerase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 3.1.1.-

Molecular weight: Translated: 54139; Mature: 54139

Theoretical pI: Translated: 9.14; Mature: 9.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLYQFHEFQRAMLSPLTAWAQAASKSFANPSSPLSLVPGATRLAAGYELLYRLGKDYEKP
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEECCCHHHHHHHHHHHHHHCCCCCCC
EFGIHQIVKDGHNIPIVEQTIIEKPFCRLLRFKRYADDSGAVGQLKDEPVVLVCAPLSGH
CHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCC
HATLLRDTVRTLLQDHKVYITDWIDARMVPAEVGSFHLDDYVGYIQKFIRHIGARNLHVI
HHHHHHHHHHHHHHCCCEEEEECCCCEECHHHHCCCHHHHHHHHHHHHHHHHCCCCEEEE
SVCQPTVPVLAAISLMASRGEDTPLTMTMMGGPIDARKSPTSVNSLATNRSHSWFENNVI
EECCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCHHHHHCCCCCCHHHCCCE
HTVPANYPGEGRKVYPGFLQHTGFVAMNPERHAQSHWDFYQSLLRGDEEDAEAHRRFYDE
EECCCCCCCCCCEECCHHHHHCCEEEECCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
YNAVLDMAAEYYLDTIRIVFQEFRLAEGTWDIHGERVKPADIHSTALMTIEGELDDISGS
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCC
GQTHVAHELCTGIEQAHRRSLTAEKCGHYGIFSGRRWRTIIYPQLRDFIREHASQPKKPT
CCHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCCCEEEEECHHHHHHHHHHHCCCCCCC
PQAGSTPNSPSTPSRSASGSETASAAPAKAAALKLSAKRTAAKTRAAKPPAAKRGAIKLA
CCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEE
APRTRKAA
CCCCCCCC
>Mature Secondary Structure
MLYQFHEFQRAMLSPLTAWAQAASKSFANPSSPLSLVPGATRLAAGYELLYRLGKDYEKP
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEECCCHHHHHHHHHHHHHHCCCCCCC
EFGIHQIVKDGHNIPIVEQTIIEKPFCRLLRFKRYADDSGAVGQLKDEPVVLVCAPLSGH
CHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCC
HATLLRDTVRTLLQDHKVYITDWIDARMVPAEVGSFHLDDYVGYIQKFIRHIGARNLHVI
HHHHHHHHHHHHHHCCCEEEEECCCCEECHHHHCCCHHHHHHHHHHHHHHHHCCCCEEEE
SVCQPTVPVLAAISLMASRGEDTPLTMTMMGGPIDARKSPTSVNSLATNRSHSWFENNVI
EECCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCHHHHHCCCCCCHHHCCCE
HTVPANYPGEGRKVYPGFLQHTGFVAMNPERHAQSHWDFYQSLLRGDEEDAEAHRRFYDE
EECCCCCCCCCCEECCHHHHHCCEEEECCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
YNAVLDMAAEYYLDTIRIVFQEFRLAEGTWDIHGERVKPADIHSTALMTIEGELDDISGS
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCC
GQTHVAHELCTGIEQAHRRSLTAEKCGHYGIFSGRRWRTIIYPQLRDFIREHASQPKKPT
CCHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCCCEEEEECHHHHHHHHHHHCCCCCCC
PQAGSTPNSPSTPSRSASGSETASAAPAKAAALKLSAKRTAAKTRAAKPPAAKRGAIKLA
CCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEE
APRTRKAA
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA