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The map label for this gene is pilB [H]

Identifier: 126452463

GI number: 126452463

Start: 3433860

End: 3435083

Strand: Direct

Name: pilB [H]

Synonym: BURPS1106A_3531

Alternate gene names: 126452463

Gene position: 3433860-3435083 (Clockwise)

Preceding gene: 126453893

Following gene: 126451673

Centisome position: 86.09

GC content: 72.14

Gene sequence:

>1224_bases
GTGCGGTCCGCAGCATCCGCGCTGCCCCGGCCGCGCGCGCCCGAGCTCGCCGACGACGCCCCCGCGGTCGGTATCGTCGC
CGACATGCTGCGCGCGGCCCACGAGCGCGATGCTTCCGACATCCATGTCGAGCCCTGTGAATCCGGCTGGCGCGTGCGTC
TGCGCATCGACGGCGTGCTGCACGAGTTCGCGCGGCCGCCCACTCATTTGCGCGACGCATGCGTGACGCGCATCAAGGTG
CTCGCGCGGATGGACATCGCCGAGCGCCGCGTGCCGCAGGACGGCCGGCTGCGGCTCCCGCTCGCGCCGGGGCGCGCGGG
CGACTACCGCGTGAGCTCGCTGCCGACGCTGCATGGCGAGAAACTCGTGCTGCGGCGTCTGGAGACGCTGCCCGCCGATC
TGTCGCTCGCCGCGCTCGGCTTCGACGCGGCTCAGGCACGCGGCGTCGAGGCGGCGATCGGCGCGCCGCACGGTCTCATC
CTCGTCACGGGGCCGACGGGCAGCGGCAAGACGCTGTCACTGTATTGCTTCCTGCAAATGCTGAACGACGTGTCGCGCAA
CGTCTGCACGGTCGAGGATCCGGCCGAGATCGAGCTCGACGGCATCAACCAGGTCGGCGTGCGCGAGAAGGCGGGCCTCA
CCTTCGCGGTCGCGCTGCGCGCGTTCCTGCGGCAGGATCCCGACGTCATCATGGTCGGCGAGATACGCGATGCGCAGACC
GCCGACGTCGCGCTGAAAGCCGCGCAGACCGGGCACCTCGTGCTGTCGACGCTGCACACGAATGACGCGCCCGCCGCGGT
AGCCCGCCTGCTCGACATCGGCGTCGCGCCGTACAACCTCGCCGCCGCGCTGCGGCTCGTGACAGCGCAGCGGCTCGTGC
GACGCCTGTGCCCCGCTTGCCGCGCGCCATCGGACGCGTCGCCCGCCACACTGCGCGCCGCGGGATTCGACGCCGCGTCG
ATCGACGCCGGCTGGCGCCCGTATGCCGCGAACGGATGCGCCGCATGCCATGGAATCGGTTATCGGGGGCGCATCGGCAT
CCATCAGGTGATGCCGCTGTCCGCCGAGTTGCGCAACCTGACCGTCGCGCGCGCGAGCAGCGCCGAACTCGCGCGACAAG
CGCGCGCCGAAGGCATGGCGAGCCTGCGCGATGCGGCACTCGCCCGCGTTCGCGACGGCACGACGAGCTTGAGCGAAGCG
CTCGCCACGACGGAGGCGGCATGA

Upstream 100 bases:

>100_bases
CCGTCCGCACGACGCGCGATGATCGTTTCATCGTGTCGAGCAGGCGGCCGCGAGGCCGGTTCCGATGTCCGCAACGCCCT
CCAACCCTTCTTTCCCCGCC

Downstream 100 bases:

>100_bases
GTACACGCGGACCGGCTTCGCCGATCGAGTCCCGATACCGTTGGGCGGGCGTGACGATCGACGGCGCGCGGCGGCGCGGC
ATGTTGATCGCCGTCGATCC

Product: type IV pilus biogenesis protein PilB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 407; Mature: 407

Protein sequence:

>407_residues
MRSAASALPRPRAPELADDAPAVGIVADMLRAAHERDASDIHVEPCESGWRVRLRIDGVLHEFARPPTHLRDACVTRIKV
LARMDIAERRVPQDGRLRLPLAPGRAGDYRVSSLPTLHGEKLVLRRLETLPADLSLAALGFDAAQARGVEAAIGAPHGLI
LVTGPTGSGKTLSLYCFLQMLNDVSRNVCTVEDPAEIELDGINQVGVREKAGLTFAVALRAFLRQDPDVIMVGEIRDAQT
ADVALKAAQTGHLVLSTLHTNDAPAAVARLLDIGVAPYNLAAALRLVTAQRLVRRLCPACRAPSDASPATLRAAGFDAAS
IDAGWRPYAANGCAACHGIGYRGRIGIHQVMPLSAELRNLTVARASSAELARQARAEGMASLRDAALARVRDGTTSLSEA
LATTEAA

Sequences:

>Translated_407_residues
MRSAASALPRPRAPELADDAPAVGIVADMLRAAHERDASDIHVEPCESGWRVRLRIDGVLHEFARPPTHLRDACVTRIKV
LARMDIAERRVPQDGRLRLPLAPGRAGDYRVSSLPTLHGEKLVLRRLETLPADLSLAALGFDAAQARGVEAAIGAPHGLI
LVTGPTGSGKTLSLYCFLQMLNDVSRNVCTVEDPAEIELDGINQVGVREKAGLTFAVALRAFLRQDPDVIMVGEIRDAQT
ADVALKAAQTGHLVLSTLHTNDAPAAVARLLDIGVAPYNLAAALRLVTAQRLVRRLCPACRAPSDASPATLRAAGFDAAS
IDAGWRPYAANGCAACHGIGYRGRIGIHQVMPLSAELRNLTVARASSAELARQARAEGMASLRDAALARVRDGTTSLSEA
LATTEAA
>Mature_407_residues
MRSAASALPRPRAPELADDAPAVGIVADMLRAAHERDASDIHVEPCESGWRVRLRIDGVLHEFARPPTHLRDACVTRIKV
LARMDIAERRVPQDGRLRLPLAPGRAGDYRVSSLPTLHGEKLVLRRLETLPADLSLAALGFDAAQARGVEAAIGAPHGLI
LVTGPTGSGKTLSLYCFLQMLNDVSRNVCTVEDPAEIELDGINQVGVREKAGLTFAVALRAFLRQDPDVIMVGEIRDAQT
ADVALKAAQTGHLVLSTLHTNDAPAAVARLLDIGVAPYNLAAALRLVTAQRLVRRLCPACRAPSDASPATLRAAGFDAAS
IDAGWRPYAANGCAACHGIGYRGRIGIHQVMPLSAELRNLTVARASSAELARQARAEGMASLRDAALARVRDGTTSLSEA
LATTEAA

Specific function: Involved in the translocation of the type IV pilin [H]

COG id: COG2804

COG function: function code NU; Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1789723, Length=382, Percent_Identity=46.0732984293194, Blast_Score=330, Evalue=8e-92,
Organism=Escherichia coli, GI1786296, Length=385, Percent_Identity=44.9350649350649, Blast_Score=313, Evalue=2e-86,
Organism=Escherichia coli, GI87082188, Length=280, Percent_Identity=35.3571428571429, Blast_Score=108, Evalue=8e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013374
- InterPro:   IPR007831
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E; PF05157 GSPII_E_N [H]

EC number: NA

Molecular weight: Translated: 43158; Mature: 43158

Theoretical pI: Translated: 8.23; Mature: 8.23

Prosite motif: PS51007 CYTC ; PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSAASALPRPRAPELADDAPAVGIVADMLRAAHERDASDIHVEPCESGWRVRLRIDGVL
CCCCHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCEEEEEEHHHH
HEFARPPTHLRDACVTRIKVLARMDIAERRVPQDGRLRLPLAPGRAGDYRVSSLPTLHGE
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCEECCCCCCCCCH
KLVLRRLETLPADLSLAALGFDAAQARGVEAAIGAPHGLILVTGPTGSGKTLSLYCFLQM
HHHHHHHHHCCCCCEEEEECCCHHHHCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHH
LNDVSRNVCTVEDPAEIELDGINQVGVREKAGLTFAVALRAFLRQDPDVIMVGEIRDAQT
HHHHHCCEEECCCCCEEEECCCCCCCCCHHCCCHHHHHHHHHHHCCCCEEEEECCCCCCH
ADVALKAAQTGHLVLSTLHTNDAPAAVARLLDIGVAPYNLAAALRLVTAQRLVRRLCPAC
HHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
RAPSDASPATLRAAGFDAASIDAGWRPYAANGCAACHGIGYRGRIGIHQVMPLSAELRNL
CCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCHHHHCCCHHHHHHE
TVARASSAELARQARAEGMASLRDAALARVRDGTTSLSEALATTEAA
EEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCC
>Mature Secondary Structure
MRSAASALPRPRAPELADDAPAVGIVADMLRAAHERDASDIHVEPCESGWRVRLRIDGVL
CCCCHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCEEEEEEHHHH
HEFARPPTHLRDACVTRIKVLARMDIAERRVPQDGRLRLPLAPGRAGDYRVSSLPTLHGE
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCEECCCCCCCCCH
KLVLRRLETLPADLSLAALGFDAAQARGVEAAIGAPHGLILVTGPTGSGKTLSLYCFLQM
HHHHHHHHHCCCCCEEEEECCCHHHHCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHH
LNDVSRNVCTVEDPAEIELDGINQVGVREKAGLTFAVALRAFLRQDPDVIMVGEIRDAQT
HHHHHCCEEECCCCCEEEECCCCCCCCCHHCCCHHHHHHHHHHHCCCCEEEEECCCCCCH
ADVALKAAQTGHLVLSTLHTNDAPAAVARLLDIGVAPYNLAAALRLVTAQRLVRRLCPAC
HHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
RAPSDASPATLRAAGFDAASIDAGWRPYAANGCAACHGIGYRGRIGIHQVMPLSAELRNL
CCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCHHHHCCCHHHHHHE
TVARASSAELARQARAEGMASLRDAALARVRDGTTSLSEALATTEAA
EEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8820654 [H]