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The map label for this gene is ispF

Identifier: 126452393

GI number: 126452393

Start: 2364126

End: 2364614

Strand: Reverse

Name: ispF

Synonym: BURPS1106A_2400

Alternate gene names: 126452393

Gene position: 2364614-2364126 (Counterclockwise)

Preceding gene: 126454537

Following gene: 126453200

Centisome position: 59.29

GC content: 68.51

Gene sequence:

>489_bases
ATGGATTTCAGAATCGGACAAGGCTACGACGTGCACCAGCTCGTTCCCGGACGCCCGCTCATCATCGGCGGCGTGACGAT
CCCTTACGAGCGGGGGCTGCTCGGCCACTCGGACGCCGACGTGCTGCTGCACGCGATCACCGACGCGCTCTTCGGCGCGG
CGGCGCTCGGCGACATCGGCCGCCACTTCTCCGACACCGATCCGCGCTTCAAGGGCGCCGACAGCCGCGCGCTGTTGCGC
GAATGCGCATCGCGCGTCGCGCAGGCGGGTTTTGCGATCCGCAACGTCGACAGCACGATCATCGCGCAGGCGCCGAAGCT
TGCGCCGCATATCGACGCGATGCGCGCGAACATCGCGGCCGATCTCGACTTGCCGCTCGATCGCGTGAACGTGAAGGCGA
AGACGAACGAGAAGCTCGGCTATCTCGGGCGCGGTGAGGGGATCGAGGCGCAGGCGGCCGCGCTCGTCGTGCGCGAAGCG
GCCGCGTGA

Upstream 100 bases:

>100_bases
TATCCGGAGGATTTCGACCTCGCGGAGGCGATCCTCGCGCAACCGGCGCGCGCTTCCTGAACCGCATCTTCACCATCGCA
TCACACTCGGATCGAATCAC

Downstream 100 bases:

>100_bases
GCGGGGGGCATGAATGGGGGCGGGGCGCGGAACGCATCGCGCGCCCGGCGCGCGCGGCGCGCTTCGCGCTCCACGCGGCG
CCGTGGCCTCGCGGCGGCCT

Product: 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Products: NA

Alternate protein names: MECDP-synthase; MECPS

Number of amino acids: Translated: 162; Mature: 162

Protein sequence:

>162_residues
MDFRIGQGYDVHQLVPGRPLIIGGVTIPYERGLLGHSDADVLLHAITDALFGAAALGDIGRHFSDTDPRFKGADSRALLR
ECASRVAQAGFAIRNVDSTIIAQAPKLAPHIDAMRANIAADLDLPLDRVNVKAKTNEKLGYLGRGEGIEAQAAALVVREA
AA

Sequences:

>Translated_162_residues
MDFRIGQGYDVHQLVPGRPLIIGGVTIPYERGLLGHSDADVLLHAITDALFGAAALGDIGRHFSDTDPRFKGADSRALLR
ECASRVAQAGFAIRNVDSTIIAQAPKLAPHIDAMRANIAADLDLPLDRVNVKAKTNEKLGYLGRGEGIEAQAAALVVREA
AA
>Mature_162_residues
MDFRIGQGYDVHQLVPGRPLIIGGVTIPYERGLLGHSDADVLLHAITDALFGAAALGDIGRHFSDTDPRFKGADSRALLR
ECASRVAQAGFAIRNVDSTIIAQAPKLAPHIDAMRANIAADLDLPLDRVNVKAKTNEKLGYLGRGEGIEAQAAALVVREA
AA

Specific function: Converts 4-diphosphocytidyl-2-C-methyl-D-erythritol 2- phosphate into 2-C-methyl-D-erythritol 2,4-cyclodiphosphate (MECDP) and CMP

COG id: COG0245

COG function: function code I; 2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispF family

Homologues:

Organism=Escherichia coli, GI1789103, Length=156, Percent_Identity=63.4615384615385, Blast_Score=185, Evalue=1e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPF_BURM7 (A3MKM3)

Other databases:

- EMBL:   CP000548
- RefSeq:   YP_001080809.1
- ProteinModelPortal:   A3MKM3
- SMR:   A3MKM3
- GeneID:   4893522
- GenomeReviews:   CP000548_GR
- KEGG:   bmn:BMA10247_1258
- TIGR:   BMA10247_1258
- HOGENOM:   HBG630070
- OMA:   HKFGGEG
- ProtClustDB:   PRK00084
- BioCyc:   BMAL320389:BMA10247_1258-MONOMER
- HAMAP:   MF_00107
- InterPro:   IPR003526
- InterPro:   IPR020555
- Gene3D:   G3DSA:3.30.1330.50
- TIGRFAMs:   TIGR00151

Pfam domain/function: PF02542 YgbB; SSF69765 YgbB_synth

EC number: =4.6.1.12

Molecular weight: Translated: 17175; Mature: 17175

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: PS01350 ISPF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDFRIGQGYDVHQLVPGRPLIIGGVTIPYERGLLGHSDADVLLHAITDALFGAAALGDIG
CCCCCCCCCCHHHCCCCCCEEEECEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
RHFSDTDPRFKGADSRALLRECASRVAQAGFAIRNVDSTIIAQAPKLAPHIDAMRANIAA
HCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCCCCHHHHHHHHHHCC
DLDLPLDRVNVKAKTNEKLGYLGRGEGIEAQAAALVVREAAA
CCCCCCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MDFRIGQGYDVHQLVPGRPLIIGGVTIPYERGLLGHSDADVLLHAITDALFGAAALGDIG
CCCCCCCCCCHHHCCCCCCEEEECEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
RHFSDTDPRFKGADSRALLRECASRVAQAGFAIRNVDSTIIAQAPKLAPHIDAMRANIAA
HCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCCCCHHHHHHHHHHCC
DLDLPLDRVNVKAKTNEKLGYLGRGEGIEAQAAALVVREAAA
CCCCCCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA