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The map label for this gene is sucB [H]

Identifier: 126452386

GI number: 126452386

Start: 1736460

End: 1737725

Strand: Direct

Name: sucB [H]

Synonym: BURPS1106A_1773

Alternate gene names: 126452386

Gene position: 1736460-1737725 (Clockwise)

Preceding gene: 126453274

Following gene: 126455059

Centisome position: 43.54

GC content: 68.09

Gene sequence:

>1266_bases
ATGGCTATCGTAGAAGTCAAAGTCCCCCAGCTTTCGGAGTCGGTTTCGGAAGCGACCATGCTGCAGTGGAAGAAGAAGCC
CGGCGAAGCCGTCGCGCAGGACGAAATCCTGATCGAGCTCGAGACGGACAAGGTCGTCCTCGAAGTGCCGGCGCCGGCCG
CGGGCGTGCTCGCGCAGGTGCTGCAGAACGACGGTGACACGGTCGTCGCCGACCAGGTGATCGCGACCATCGACACCGAA
GCGAAGGCAGGCGCCGCCGCGGCAGCGGCAGGCGCCGCCGACGTTCAGCCGGCCGCCGCTCCCGTCGCCGCGCCGGCGCC
CGCCGCACAGCCGGCCGCCGCCGCCGCGTCGAGCACCGCGGCCGCATCGCCCGCCGCGTCGAAGCTGATGGCCGAGAAGG
GCATCGGCGCCGGCGACGTCGCCGGCTCGGGCCGCGACGGCCGCATCACGAAGGGCGACGTGCTGTCCGCGGGCAGCGCG
CCGGCCGCCGCGCCGGCGAAGGCCGCCGCGAAGCCGGCGCTGCCGGAAGTGAAGGTGCCGGCATCGGCGACGACGTGGCT
CAACGACCGTCCGGAGCAGCGCGTGCCGATGTCGCGCCTGCGCGCGCGCATCGCCGAGCGTCTGCTCGAATCGCAGCAGA
CCAACGCGATCCTCACGACGTTCAACGAAGTGAACATGCAGCCGGTGATGGACCTGCGCGCGAAGTACAAGGACAAGTTC
GAGAAGGAGCACGGCGTGAAGCTCGGCTTCATGTCGTTCTTCGTGAAGGCGGCCGTTCACGCGCTGAAGAAGTTCCCGCT
CGTGAACGCGTCGATCGACGGCAACGACATCGTCTACCACGGCTACTTCGACATCGGCATCGCGGTCGGTTCGCCGCGCG
GCCTCGTCGTGCCGATCCTGCGCAACGCGGATCAGCTGAGCCTCGCCGAGATCGAGAAGAAGATCGCCGAATTCGGCCAG
AAGGCAAAGGACGGCAAGCTGTCGATCGAGGAAATGACGGGCGGCACGTTCTCGATCTCGAACGGCGGCGTGTTCGGCTC
GATGCTGTCGACGCCGATCATCAACCCGCCGCAGTCGGCGATCCTCGGCGTGCACGCGACGAAGGAGCGCCCGGTCGTCG
AGAACGGCCAGATCGTGATCCGCCCGATCAACTATCTCGCGCTGTCGTACGACCACCGGATCATCGACGGTCGCGAAGCG
GTGCTGTCGCTCGTCGCGATGAAGGACGCGCTCGAAGATCCGGCGCGCCTGCTGCTCGACCTGTAA

Upstream 100 bases:

>100_bases
TTCCCGCGTGCTTTCGGACGCCGCGCGCCGCGCGCCGTGATACCCGACAGCGCACGCGCCAGTCTCGTCGTTACCCAGAT
TAAGCATCCAGGAAATTCAC

Downstream 100 bases:

>100_bases
GCACGCTTTTCATACCGTATCGAACCGCTCAGGCGGGCCGCGCGGGCGCCCATTGCGAGGCGCCTGCTCGGCTTGCCGGA
GCCCAATCAAAGGATTGTCA

Product: dihydrolipoamide succinyltransferase

Products: NA

Alternate protein names: 2-oxoglutarate dehydrogenase complex component E2; OGDC-E2; Dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenase complex [H]

Number of amino acids: Translated: 421; Mature: 420

Protein sequence:

>421_residues
MAIVEVKVPQLSESVSEATMLQWKKKPGEAVAQDEILIELETDKVVLEVPAPAAGVLAQVLQNDGDTVVADQVIATIDTE
AKAGAAAAAAGAADVQPAAAPVAAPAPAAQPAAAAASSTAAASPAASKLMAEKGIGAGDVAGSGRDGRITKGDVLSAGSA
PAAAPAKAAAKPALPEVKVPASATTWLNDRPEQRVPMSRLRARIAERLLESQQTNAILTTFNEVNMQPVMDLRAKYKDKF
EKEHGVKLGFMSFFVKAAVHALKKFPLVNASIDGNDIVYHGYFDIGIAVGSPRGLVVPILRNADQLSLAEIEKKIAEFGQ
KAKDGKLSIEEMTGGTFSISNGGVFGSMLSTPIINPPQSAILGVHATKERPVVENGQIVIRPINYLALSYDHRIIDGREA
VLSLVAMKDALEDPARLLLDL

Sequences:

>Translated_421_residues
MAIVEVKVPQLSESVSEATMLQWKKKPGEAVAQDEILIELETDKVVLEVPAPAAGVLAQVLQNDGDTVVADQVIATIDTE
AKAGAAAAAAGAADVQPAAAPVAAPAPAAQPAAAAASSTAAASPAASKLMAEKGIGAGDVAGSGRDGRITKGDVLSAGSA
PAAAPAKAAAKPALPEVKVPASATTWLNDRPEQRVPMSRLRARIAERLLESQQTNAILTTFNEVNMQPVMDLRAKYKDKF
EKEHGVKLGFMSFFVKAAVHALKKFPLVNASIDGNDIVYHGYFDIGIAVGSPRGLVVPILRNADQLSLAEIEKKIAEFGQ
KAKDGKLSIEEMTGGTFSISNGGVFGSMLSTPIINPPQSAILGVHATKERPVVENGQIVIRPINYLALSYDHRIIDGREA
VLSLVAMKDALEDPARLLLDL
>Mature_420_residues
AIVEVKVPQLSESVSEATMLQWKKKPGEAVAQDEILIELETDKVVLEVPAPAAGVLAQVLQNDGDTVVADQVIATIDTEA
KAGAAAAAAGAADVQPAAAPVAAPAPAAQPAAAAASSTAAASPAASKLMAEKGIGAGDVAGSGRDGRITKGDVLSAGSAP
AAAPAKAAAKPALPEVKVPASATTWLNDRPEQRVPMSRLRARIAERLLESQQTNAILTTFNEVNMQPVMDLRAKYKDKFE
KEHGVKLGFMSFFVKAAVHALKKFPLVNASIDGNDIVYHGYFDIGIAVGSPRGLVVPILRNADQLSLAEIEKKIAEFGQK
AKDGKLSIEEMTGGTFSISNGGVFGSMLSTPIINPPQSAILGVHATKERPVVENGQIVIRPINYLALSYDHRIIDGREAV
LSLVAMKDALEDPARLLLDL

Specific function: The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of three enzymatic components:2- oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2)

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI19923748, Length=234, Percent_Identity=56.4102564102564, Blast_Score=285, Evalue=5e-77,
Organism=Homo sapiens, GI31711992, Length=442, Percent_Identity=31.447963800905, Blast_Score=174, Evalue=2e-43,
Organism=Homo sapiens, GI110671329, Length=437, Percent_Identity=27.6887871853547, Blast_Score=160, Evalue=2e-39,
Organism=Homo sapiens, GI203098753, Length=469, Percent_Identity=28.997867803838, Blast_Score=154, Evalue=2e-37,
Organism=Homo sapiens, GI203098816, Length=469, Percent_Identity=28.997867803838, Blast_Score=152, Evalue=4e-37,
Organism=Homo sapiens, GI260898739, Length=161, Percent_Identity=37.888198757764, Blast_Score=100, Evalue=2e-21,
Organism=Escherichia coli, GI1786946, Length=424, Percent_Identity=52.3584905660377, Blast_Score=442, Evalue=1e-125,
Organism=Escherichia coli, GI1786305, Length=430, Percent_Identity=32.3255813953488, Blast_Score=164, Evalue=9e-42,
Organism=Caenorhabditis elegans, GI25146366, Length=425, Percent_Identity=43.0588235294118, Blast_Score=327, Evalue=6e-90,
Organism=Caenorhabditis elegans, GI17560088, Length=447, Percent_Identity=31.3199105145414, Blast_Score=182, Evalue=2e-46,
Organism=Caenorhabditis elegans, GI17537937, Length=430, Percent_Identity=27.4418604651163, Blast_Score=162, Evalue=3e-40,
Organism=Caenorhabditis elegans, GI17538894, Length=325, Percent_Identity=31.6923076923077, Blast_Score=137, Evalue=1e-32,
Organism=Saccharomyces cerevisiae, GI6320352, Length=414, Percent_Identity=43.2367149758454, Blast_Score=328, Evalue=7e-91,
Organism=Saccharomyces cerevisiae, GI6324258, Length=454, Percent_Identity=30.1762114537445, Blast_Score=150, Evalue=3e-37,
Organism=Drosophila melanogaster, GI24645909, Length=242, Percent_Identity=55.3719008264463, Blast_Score=283, Evalue=2e-76,
Organism=Drosophila melanogaster, GI18859875, Length=441, Percent_Identity=27.891156462585, Blast_Score=152, Evalue=3e-37,
Organism=Drosophila melanogaster, GI24582497, Length=228, Percent_Identity=34.6491228070175, Blast_Score=132, Evalue=3e-31,
Organism=Drosophila melanogaster, GI20129315, Length=228, Percent_Identity=34.6491228070175, Blast_Score=132, Evalue=4e-31,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053
- InterPro:   IPR006255 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.61 [H]

Molecular weight: Translated: 43947; Mature: 43816

Theoretical pI: Translated: 5.53; Mature: 5.53

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIVEVKVPQLSESVSEATMLQWKKKPGEAVAQDEILIELETDKVVLEVPAPAAGVLAQV
CEEEEEECCHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCEEEEECCCCHHHHHHHH
LQNDGDTVVADQVIATIDTEAKAGAAAAAAGAADVQPAAAPVAAPAPAAQPAAAAASSTA
HHCCCCEEEHHHHHHEECCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCC
AASPAASKLMAEKGIGAGDVAGSGRDGRITKGDVLSAGSAPAAAPAKAAAKPALPEVKVP
CCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEECCCCCCCCCCHHHHCCCCCCCEECC
ASATTWLNDRPEQRVPMSRLRARIAERLLESQQTNAILTTFNEVNMQPVMDLRAKYKDKF
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEHHHCCCCHHHHHHHHHHHHH
EKEHGVKLGFMSFFVKAAVHALKKFPLVNASIDGNDIVYHGYFDIGIAVGSPRGLVVPIL
HHHHCCEEHHHHHHHHHHHHHHHHCCCEECCCCCCEEEEEEEEEEEEEECCCCCEEEEEE
RNADQLSLAEIEKKIAEFGQKAKDGKLSIEEMTGGTFSISNGGVFGSMLSTPIINPPQSA
CCCCCHHHHHHHHHHHHHCCCCCCCCEEEEECCCCEEEECCCCEEHHHHCCCCCCCCHHH
ILGVHATKERPVVENGQIVIRPINYLALSYDHRIIDGREAVLSLVAMKDALEDPARLLLD
EEEEECCCCCCCCCCCCEEEEECCEEEEECCCEEECCHHHHHHHHHHHHHHCCHHHHHHC
L
C
>Mature Secondary Structure 
AIVEVKVPQLSESVSEATMLQWKKKPGEAVAQDEILIELETDKVVLEVPAPAAGVLAQV
EEEEEECCHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCEEEEECCCCHHHHHHHH
LQNDGDTVVADQVIATIDTEAKAGAAAAAAGAADVQPAAAPVAAPAPAAQPAAAAASSTA
HHCCCCEEEHHHHHHEECCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCC
AASPAASKLMAEKGIGAGDVAGSGRDGRITKGDVLSAGSAPAAAPAKAAAKPALPEVKVP
CCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEECCCCCCCCCCHHHHCCCCCCCEECC
ASATTWLNDRPEQRVPMSRLRARIAERLLESQQTNAILTTFNEVNMQPVMDLRAKYKDKF
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEHHHCCCCHHHHHHHHHHHHH
EKEHGVKLGFMSFFVKAAVHALKKFPLVNASIDGNDIVYHGYFDIGIAVGSPRGLVVPIL
HHHHCCEEHHHHHHHHHHHHHHHHCCCEECCCCCCEEEEEEEEEEEEEECCCCCEEEEEE
RNADQLSLAEIEKKIAEFGQKAKDGKLSIEEMTGGTFSISNGGVFGSMLSTPIINPPQSA
CCCCCHHHHHHHHHHHHHCCCCCCCCEEEEECCCCEEEECCCCEEHHHHCCCCCCCCHHH
ILGVHATKERPVVENGQIVIRPINYLALSYDHRIIDGREAVLSLVAMKDALEDPARLLLD
EEEEECCCCCCCCCCCCEEEEECCEEEEECCCEEECCHHHHHHHHHHHHHHCCHHHHHHC
L
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8867378 [H]