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The map label for this gene is ilvB [H]

Identifier: 126452197

GI number: 126452197

Start: 1253365

End: 1255128

Strand: Direct

Name: ilvB [H]

Synonym: BURPS1106A_1281

Alternate gene names: 126452197

Gene position: 1253365-1255128 (Clockwise)

Preceding gene: 126455107

Following gene: 126454468

Centisome position: 31.42

GC content: 63.66

Gene sequence:

>1764_bases
ATGAACATGCCCAGCGCGGAATTCTCCACGTCGGAATCCCTTTCCCCTCAGAAAACCGGCTCCATCGGCGCCACCGTGCT
CATGAAGGCACTGGCCGACGAGAACGTCGAATTCATCTGGGGCTACCCTGGCGGCTCGGTCCTCTACATCTACGACGAGC
TGTACAAGCAAGACAAGATTCAACACGTGCTCGTGCGCCACGAGCAGGCCGCCGTGCACGCCGCGGACGCGTACTCGCGC
TCGACGGGCAAGGTCGGCGTGTGTCTCGTCACATCCGGCCCGGGCGTGACGAACGCGGTGACGGGCATCGCCACCGCCTA
CATGGATTCGATCCCGCTCGTCGTGATCAGCGGCCAGGTGCCGACCGCGGCGATCGGCCAGGACGCGTTCCAGGAGTGCG
ATACCGTTGGCATCACGCGGCCGTGCGTGAAGCACAATTTCCTCGTGAAGGACGTGCGCGAGCTCGCGCAAACCGTCAAG
AAGGCGTTCTACATCGCGCGTACCGGCCGGCCCGGTCCGGTCCTCATCGACATCCCGAAGGACATCTCGAAGACGCCGTG
CGAGTACGAGCCCGTCAAGAACGTGTCGCTGCGCTCGTACAACCCGGTCACGAAAGGCCATTCGGGGCAGATCCGCAAGG
CGGTGTCGCTGCTGCTGTCCGCGAAGCGTCCGTACATCTACACGGGCGGCGGCATCATCCTCGCCGACGCGTCGCGCGAG
CTGAACCAGTTCGCCGACCTGCTCGGCTATCCCGTCACGAACACGCTGATGGGCCTCGGCGGCTATCGCTCGTCGGACAG
GAAATTCCTCGGCATGCTCGGCATGCACGGCACCTACGAAGCGAACATGGCGATGCAGCACTGCGACGTGTTGATCGCGA
TCGGCGCGCGCTTCGACGACCGCGTGATCGGCGATCCGGCGCACTTCGCGTCGCGCCCGCGCAAGATCATCCATATCGAC
ATCGATCCGTCGTCGATCTCGAAGCGCGTGAAGGTCGACATCCCGATCGTCGGCGACGTGAAGGAAGTGCTCAAGGAGCT
GATCGAGCAGTTGCAGACGGCCGAGCACGGCCCGGACGCCGACGCGCTCGCGCAGTGGTGGAAGGAAATCGAAGGCTGGC
GCGGGACGGATTGCCTGAAGTACGACCGCGCGAGCGAGATCATCAAGCCGCAATACGTCGTCGAGAAGCTCTGGGAGCTC
ACCGACGGCAACGCGTTCGTCTGCTCGGACGTCGGCCAGCACCAGATGTGGGCCGCGCAGTTCTACCGCTTCAACAAGCC
GCGCCGCTGGATCAACTCGGGCGGCCTCGGCACGATGGGCTTCGGCCTGCCCGCCGCGATGGGCGTCAAGATGGCGCACC
CGGACGACGACGTCGTCTGCATCACGGGCGAGGGCTCGATCCAGATGTGCATCCAGGAGCTGTCGACCTGCAAGCAGTAC
GACACTCCGGTGAAGATCATTTCGCTGAACAACCGCTACCTCGGCATGGTCCGCCAATGGCAGCAGATCGAATACAGCAA
GCGCTATTCGCATTCGTACATGGATGCGCTGCCCGATTTCGTGAAGCTCGCGCAGGCGTACGGCCATATCGGGATGCGCA
TCGAAAAATCCGCGGACGTCGAGCCGGCGCTCAAGGAAGCGCTGAGCCTGAAGGACCGCACCGTGTTTCTCGACTTCCAG
ACCGACCCCACCGAGAACGTCTGGCCGATGGTCCAGGCCGGCAAGGGCATCACCGAGATGCTGCTCGGATCGGAAGATCT
GTAA

Upstream 100 bases:

>100_bases
TGAAGCCCGCCTTTCAACTGGACGCGCCGCTTGAACCCGAGCCACAAGCCCGGCAGAGAGCACCCGATCAATTTTTTGCC
GAAAATTCGAAAGGTCAAAG

Downstream 100 bases:

>100_bases
CGGCGCGATGCGCTCGAAGCCGCGGGCGGCCGCCTTCACGAAGGGCGGCGCGGCACGCGGCGGGCGCCGCGCGACACGCA
ACGACACGGGATTATTCTGG

Product: acetolactate synthase 3 catalytic subunit

Products: NA

Alternate protein names: AHAS-III; ALS-III; Acetohydroxy-acid synthase III large subunit [H]

Number of amino acids: Translated: 587; Mature: 587

Protein sequence:

>587_residues
MNMPSAEFSTSESLSPQKTGSIGATVLMKALADENVEFIWGYPGGSVLYIYDELYKQDKIQHVLVRHEQAAVHAADAYSR
STGKVGVCLVTSGPGVTNAVTGIATAYMDSIPLVVISGQVPTAAIGQDAFQECDTVGITRPCVKHNFLVKDVRELAQTVK
KAFYIARTGRPGPVLIDIPKDISKTPCEYEPVKNVSLRSYNPVTKGHSGQIRKAVSLLLSAKRPYIYTGGGIILADASRE
LNQFADLLGYPVTNTLMGLGGYRSSDRKFLGMLGMHGTYEANMAMQHCDVLIAIGARFDDRVIGDPAHFASRPRKIIHID
IDPSSISKRVKVDIPIVGDVKEVLKELIEQLQTAEHGPDADALAQWWKEIEGWRGTDCLKYDRASEIIKPQYVVEKLWEL
TDGNAFVCSDVGQHQMWAAQFYRFNKPRRWINSGGLGTMGFGLPAAMGVKMAHPDDDVVCITGEGSIQMCIQELSTCKQY
DTPVKIISLNNRYLGMVRQWQQIEYSKRYSHSYMDALPDFVKLAQAYGHIGMRIEKSADVEPALKEALSLKDRTVFLDFQ
TDPTENVWPMVQAGKGITEMLLGSEDL

Sequences:

>Translated_587_residues
MNMPSAEFSTSESLSPQKTGSIGATVLMKALADENVEFIWGYPGGSVLYIYDELYKQDKIQHVLVRHEQAAVHAADAYSR
STGKVGVCLVTSGPGVTNAVTGIATAYMDSIPLVVISGQVPTAAIGQDAFQECDTVGITRPCVKHNFLVKDVRELAQTVK
KAFYIARTGRPGPVLIDIPKDISKTPCEYEPVKNVSLRSYNPVTKGHSGQIRKAVSLLLSAKRPYIYTGGGIILADASRE
LNQFADLLGYPVTNTLMGLGGYRSSDRKFLGMLGMHGTYEANMAMQHCDVLIAIGARFDDRVIGDPAHFASRPRKIIHID
IDPSSISKRVKVDIPIVGDVKEVLKELIEQLQTAEHGPDADALAQWWKEIEGWRGTDCLKYDRASEIIKPQYVVEKLWEL
TDGNAFVCSDVGQHQMWAAQFYRFNKPRRWINSGGLGTMGFGLPAAMGVKMAHPDDDVVCITGEGSIQMCIQELSTCKQY
DTPVKIISLNNRYLGMVRQWQQIEYSKRYSHSYMDALPDFVKLAQAYGHIGMRIEKSADVEPALKEALSLKDRTVFLDFQ
TDPTENVWPMVQAGKGITEMLLGSEDL
>Mature_587_residues
MNMPSAEFSTSESLSPQKTGSIGATVLMKALADENVEFIWGYPGGSVLYIYDELYKQDKIQHVLVRHEQAAVHAADAYSR
STGKVGVCLVTSGPGVTNAVTGIATAYMDSIPLVVISGQVPTAAIGQDAFQECDTVGITRPCVKHNFLVKDVRELAQTVK
KAFYIARTGRPGPVLIDIPKDISKTPCEYEPVKNVSLRSYNPVTKGHSGQIRKAVSLLLSAKRPYIYTGGGIILADASRE
LNQFADLLGYPVTNTLMGLGGYRSSDRKFLGMLGMHGTYEANMAMQHCDVLIAIGARFDDRVIGDPAHFASRPRKIIHID
IDPSSISKRVKVDIPIVGDVKEVLKELIEQLQTAEHGPDADALAQWWKEIEGWRGTDCLKYDRASEIIKPQYVVEKLWEL
TDGNAFVCSDVGQHQMWAAQFYRFNKPRRWINSGGLGTMGFGLPAAMGVKMAHPDDDVVCITGEGSIQMCIQELSTCKQY
DTPVKIISLNNRYLGMVRQWQQIEYSKRYSHSYMDALPDFVKLAQAYGHIGMRIEKSADVEPALKEALSLKDRTVFLDFQ
TDPTENVWPMVQAGKGITEMLLGSEDL

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=585, Percent_Identity=25.6410256410256, Blast_Score=175, Evalue=1e-43,
Organism=Homo sapiens, GI21361361, Length=511, Percent_Identity=27.3972602739726, Blast_Score=156, Evalue=6e-38,
Organism=Escherichia coli, GI87081685, Length=571, Percent_Identity=57.6182136602452, Blast_Score=679, Evalue=0.0,
Organism=Escherichia coli, GI1790104, Length=560, Percent_Identity=41.0714285714286, Blast_Score=418, Evalue=1e-118,
Organism=Escherichia coli, GI1786717, Length=534, Percent_Identity=34.0823970037453, Blast_Score=315, Evalue=7e-87,
Organism=Escherichia coli, GI1787096, Length=566, Percent_Identity=28.0918727915194, Blast_Score=208, Evalue=8e-55,
Organism=Escherichia coli, GI1788716, Length=563, Percent_Identity=23.4458259325044, Blast_Score=141, Evalue=1e-34,
Organism=Caenorhabditis elegans, GI17531299, Length=550, Percent_Identity=26.3636363636364, Blast_Score=157, Evalue=1e-38,
Organism=Caenorhabditis elegans, GI17531301, Length=550, Percent_Identity=26.3636363636364, Blast_Score=157, Evalue=1e-38,
Organism=Caenorhabditis elegans, GI17542570, Length=517, Percent_Identity=26.6924564796905, Blast_Score=140, Evalue=3e-33,
Organism=Saccharomyces cerevisiae, GI6323755, Length=586, Percent_Identity=42.320819112628, Blast_Score=456, Evalue=1e-129,
Organism=Saccharomyces cerevisiae, GI6320816, Length=476, Percent_Identity=25.6302521008403, Blast_Score=107, Evalue=5e-24,
Organism=Saccharomyces cerevisiae, GI6321524, Length=518, Percent_Identity=21.042471042471, Blast_Score=84, Evalue=5e-17,
Organism=Saccharomyces cerevisiae, GI6320123, Length=544, Percent_Identity=20.2205882352941, Blast_Score=81, Evalue=5e-16,
Organism=Saccharomyces cerevisiae, GI6323073, Length=488, Percent_Identity=22.3360655737705, Blast_Score=77, Evalue=5e-15,
Organism=Drosophila melanogaster, GI19922626, Length=567, Percent_Identity=26.1022927689594, Blast_Score=179, Evalue=6e-45,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 64867; Mature: 64867

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNMPSAEFSTSESLSPQKTGSIGATVLMKALADENVEFIWGYPGGSVLYIYDELYKQDKI
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCEEEEEHHHHHHHHH
QHVLVRHEQAAVHAADAYSRSTGKVGVCLVTSGPGVTNAVTGIATAYMDSIPLVVISGQV
HHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHHHHHHCCCCEEEEECCC
PTAAIGQDAFQECDTVGITRPCVKHNFLVKDVRELAQTVKKAFYIARTGRPGPVLIDIPK
CCHHHCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCEEEECCC
DISKTPCEYEPVKNVSLRSYNPVTKGHSGQIRKAVSLLLSAKRPYIYTGGGIILADASRE
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEECCCEEEECCHHH
LNQFADLLGYPVTNTLMGLGGYRSSDRKFLGMLGMHGTYEANMAMQHCDVLIAIGARFDD
HHHHHHHHCCCHHHHHHHCCCCCCCCHHHHHHHCCCCCCHHHHHHHHCCEEEEECCCCCC
RVIGDPAHFASRPRKIIHIDIDPSSISKRVKVDIPIVGDVKEVLKELIEQLQTAEHGPDA
CCCCCCHHHHCCCCEEEEEEECHHHHCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCH
DALAQWWKEIEGWRGTDCLKYDRASEIIKPQYVVEKLWELTDGNAFVCSDVGQHQMWAAQ
HHHHHHHHHHCCCCCCCHHHHCCHHHHCCHHHHHHHHHHCCCCCEEEECCCCCHHHHHHH
FYRFNKPRRWINSGGLGTMGFGLPAAMGVKMAHPDDDVVCITGEGSIQMCIQELSTCKQY
HHHCCCCHHHHCCCCCCCCCCCCHHHHCEEEECCCCCEEEEECCCHHHHHHHHHHHHHHC
DTPVKIISLNNRYLGMVRQWQQIEYSKRYSHSYMDALPDFVKLAQAYGHIGMRIEKSADV
CCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCC
EPALKEALSLKDRTVFLDFQTDPTENVWPMVQAGKGITEMLLGSEDL
CHHHHHHHCCCCCEEEEEECCCCCCCCCHHHHCCCCHHHHHCCCCCC
>Mature Secondary Structure
MNMPSAEFSTSESLSPQKTGSIGATVLMKALADENVEFIWGYPGGSVLYIYDELYKQDKI
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCEEEEEHHHHHHHHH
QHVLVRHEQAAVHAADAYSRSTGKVGVCLVTSGPGVTNAVTGIATAYMDSIPLVVISGQV
HHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHHHHHHCCCCEEEEECCC
PTAAIGQDAFQECDTVGITRPCVKHNFLVKDVRELAQTVKKAFYIARTGRPGPVLIDIPK
CCHHHCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCEEEECCC
DISKTPCEYEPVKNVSLRSYNPVTKGHSGQIRKAVSLLLSAKRPYIYTGGGIILADASRE
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEECCCEEEECCHHH
LNQFADLLGYPVTNTLMGLGGYRSSDRKFLGMLGMHGTYEANMAMQHCDVLIAIGARFDD
HHHHHHHHCCCHHHHHHHCCCCCCCCHHHHHHHCCCCCCHHHHHHHHCCEEEEECCCCCC
RVIGDPAHFASRPRKIIHIDIDPSSISKRVKVDIPIVGDVKEVLKELIEQLQTAEHGPDA
CCCCCCHHHHCCCCEEEEEEECHHHHCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCH
DALAQWWKEIEGWRGTDCLKYDRASEIIKPQYVVEKLWELTDGNAFVCSDVGQHQMWAAQ
HHHHHHHHHHCCCCCCCHHHHCCHHHHCCHHHHHHHHHHCCCCCEEEECCCCCHHHHHHH
FYRFNKPRRWINSGGLGTMGFGLPAAMGVKMAHPDDDVVCITGEGSIQMCIQELSTCKQY
HHHCCCCHHHHCCCCCCCCCCCCHHHHCEEEECCCCCEEEEECCCHHHHHHHHHHHHHHC
DTPVKIISLNNRYLGMVRQWQQIEYSKRYSHSYMDALPDFVKLAQAYGHIGMRIEKSADV
CCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCC
EPALKEALSLKDRTVFLDFQTDPTENVWPMVQAGKGITEMLLGSEDL
CHHHHHHHCCCCCEEEEEECCCCCCCCCHHHHCCCCHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6308579; 1630901; 9278503; 3891724; 9298646 [H]