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The map label for this gene is gloA [H]

Identifier: 126452193

GI number: 126452193

Start: 689764

End: 690153

Strand: Direct

Name: gloA [H]

Synonym: BURPS1106A_0712

Alternate gene names: 126452193

Gene position: 689764-690153 (Clockwise)

Preceding gene: 126452897

Following gene: 126452599

Centisome position: 17.29

GC content: 61.79

Gene sequence:

>390_bases
ATGCGTTTGCTTCACACGATGCTGCGAGTCGGCGATCTCGACCGCTCGATCAAGTTCTACACCGAGCTGCTCGGCATGAA
ATTGCTGCGCCGCCAAGATTATCCGGACGGGAAGTTCACGCTCGCGTTCGTCGGCTATGGCGACGAGCGGGACCACACGG
TCATCGAGCTCACCCACAACTGGGACACGAAATCGTACGAGCTCGGCACGGGCTTCGGCCATCTGGCGCTCGAAGTCGAA
GACGCGTACAAGGCGTGCGAGCAGATCAAGGCGCAGGGCGGCAAGGTCACGCGCGAGGCCGGCCCGATGAAGCACGGCAC
GACGGTGATCGCGTTCGTCGAGGATCCGGACGGCTACAAGATCGAGTTCATCCAGCGCAACGCTCACTGA

Upstream 100 bases:

>100_bases
TATCGCCCCTGCTGCGCGGATGGGCGATGCGGCATCCGGCGGGAATCGTGTGGGTCTGGTACATTCGCACGTTCAAGCAA
GTCCCCACGGAGTACCCATC

Downstream 100 bases:

>100_bases
GCGCTGTCTCGCCGGCGCGGCGTGCCGCGCCGGCCGGCGGCCGGGCGCGCGATGTGCTCGGGCGGCCGCGCGCGGCCTGT
TGCATGACCGGGGCAAGACG

Product: lactoylglutathione lyase

Products: NA

Alternate protein names: Aldoketomutase; Glyoxalase I; Glx I; Ketone-aldehyde mutase; Methylglyoxalase; S-D-lactoylglutathione methylglyoxal lyase [H]

Number of amino acids: Translated: 129; Mature: 129

Protein sequence:

>129_residues
MRLLHTMLRVGDLDRSIKFYTELLGMKLLRRQDYPDGKFTLAFVGYGDERDHTVIELTHNWDTKSYELGTGFGHLALEVE
DAYKACEQIKAQGGKVTREAGPMKHGTTVIAFVEDPDGYKIEFIQRNAH

Sequences:

>Translated_129_residues
MRLLHTMLRVGDLDRSIKFYTELLGMKLLRRQDYPDGKFTLAFVGYGDERDHTVIELTHNWDTKSYELGTGFGHLALEVE
DAYKACEQIKAQGGKVTREAGPMKHGTTVIAFVEDPDGYKIEFIQRNAH
>Mature_129_residues
MRLLHTMLRVGDLDRSIKFYTELLGMKLLRRQDYPDGKFTLAFVGYGDERDHTVIELTHNWDTKSYELGTGFGHLALEVE
DAYKACEQIKAQGGKVTREAGPMKHGTTVIAFVEDPDGYKIEFIQRNAH

Specific function: Catalyzes the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S-lactoylglutathione [H]

COG id: COG0346

COG function: function code E; Lactoylglutathione lyase and related lyases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glyoxalase I family [H]

Homologues:

Organism=Homo sapiens, GI118402586, Length=151, Percent_Identity=38.4105960264901, Blast_Score=99, Evalue=1e-21,
Organism=Homo sapiens, GI217330598, Length=137, Percent_Identity=29.1970802919708, Blast_Score=64, Evalue=4e-11,
Organism=Escherichia coli, GI1787940, Length=125, Percent_Identity=68.8, Blast_Score=184, Evalue=1e-48,
Organism=Caenorhabditis elegans, GI17552228, Length=133, Percent_Identity=26.3157894736842, Blast_Score=65, Evalue=9e-12,
Organism=Saccharomyces cerevisiae, GI6323639, Length=139, Percent_Identity=37.410071942446, Blast_Score=95, Evalue=3e-21,
Organism=Drosophila melanogaster, GI20129743, Length=151, Percent_Identity=37.7483443708609, Blast_Score=91, Evalue=3e-19,
Organism=Drosophila melanogaster, GI19920404, Length=136, Percent_Identity=29.4117647058824, Blast_Score=71, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004360
- InterPro:   IPR004361
- InterPro:   IPR018146 [H]

Pfam domain/function: PF00903 Glyoxalase [H]

EC number: =4.4.1.5 [H]

Molecular weight: Translated: 14734; Mature: 14734

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS00934 GLYOXALASE_I_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLLHTMLRVGDLDRSIKFYTELLGMKLLRRQDYPDGKFTLAFVGYGDERDHTVIELTHN
CHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCEEEEEEEC
WDTKSYELGTGFGHLALEVEDAYKACEQIKAQGGKVTREAGPMKHGTTVIAFVEDPDGYK
CCCCEEECCCCCCEEEEEEHHHHHHHHHHHHCCCEEECCCCCCCCCCEEEEEEECCCCCE
IEFIQRNAH
EEEEEECCC
>Mature Secondary Structure
MRLLHTMLRVGDLDRSIKFYTELLGMKLLRRQDYPDGKFTLAFVGYGDERDHTVIELTHN
CHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCEEEEEEEC
WDTKSYELGTGFGHLALEVEDAYKACEQIKAQGGKVTREAGPMKHGTTVIAFVEDPDGYK
CCCCEEECCCCCCEEEEEEHHHHHHHHHHHHCCCEEECCCCCCCCCCEEEEEEECCCCCE
IEFIQRNAH
EEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10761919 [H]