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The map label for this gene is proP [H]

Identifier: 126452173

GI number: 126452173

Start: 1448522

End: 1450126

Strand: Reverse

Name: proP [H]

Synonym: BURPS1106A_1492

Alternate gene names: 126452173

Gene position: 1450126-1448522 (Counterclockwise)

Preceding gene: 126455280

Following gene: 126453543

Centisome position: 36.36

GC content: 70.34

Gene sequence:

>1605_bases
ATGCGCCCTTTCACGCTCGCGACCGACCCTCGTCCGATCGCGAGCGGCACTCCCGGCAAACCGTGGCGAACCGCGCCCCG
TCGCGCGGTTCGGCCGCCGTCACAGGAGTTTTCGACCTTGACTGCAACACCCGCCCCCTCCAGTTCGTCCAGCGCGCCCA
CCGAAGGCGCGCTTCCCGCCGCTGCGCACGAGATCACCGTCGTCGATCAGGGCCTGCTCAAGCGCGCCGTCGGCGCGATG
GCGCTCGGCAACGCGATGGAATGGTTCGACTTCGGCGTCTACAGCTACATCGCCGTCACGCTCGGCCAGGTGTTCTTCCC
GTCGAGCAGCCCGTCCGCGCAGTTGCTCGCGACGTTCGGCACGTTCGCCGCCGCCTTCCTCGTGCGCCCGCTCGGCGGGA
TGGTGTTCGGGCCGCTCGGCGATCGCATCGGCCGCCAGCGCGTGCTCGCGATGACGATGATCATGATGGCGGTCGGCACG
TTCGCGATCGGCCTGATCCCGAGCTACGACTCGATCGGCCTCCTCGCGCCCGTGCTGCTCCTCGTCGCGCGTCTCGTGCA
AGGCTTCTCGACGGGCGGCGAGTACGGCGGCGCGGCAACCTTCATCGCCGAGTTCTCGACCGACAAGCGCCGCGGCTTCA
TGGGCAGCTTCCTCGAGTTCGGCACGCTGATCGGCTATGTGATGGGCGCGGGCGTCGTCGCGCTGCTGACGGCTTCGCTG
TCGCACGACGCGCTGCTGTCGTGGGGCTGGCGCGTGCCGTTCCTGATCGCCGGCCCGCTCGGCCTGATCGGCCTGTACAT
CCGGATGAGGCTCGAGGAAACGCCCGCGTTCAAGCGGCAGGCCGAAGCGCGCGAAGCGCAGGACAAGGCCGTGCCGAAGG
CGCATTTCCGCCGACAGCTCGCGCGGCACTGGCGCGCGCTGCTGCTGTGCGTCGGCCTCGTGCTGATCTTCAACGTCACC
GATTACATGGCGCTGTCGTACCTGCCGAGCTATCTGTCGTCGACGCTGCACTTCGACGAGGCGCACGGCCTCGTGCTGAT
CCTGATCGTGATGGTGCTGATGATGCCGATGACGCTCGCCACGGGCCGCCTGTCGGACGCCGTCGGCCGCAAGCCGGTGA
TGCTCGCCGGCTGCGTCGGGCTCTTCGCGCTCGCGATTCCCGCGCTGCTCCTGATCCGCACCGGCGAGACGGCGCTCGTG
TTCGGCGGCCTGCTGATCCTCGGCGCACTGCTGTCGTGCTTCACGGGCGTGATGCCGTCGGCGCTGCCCGCGCTCTTTCC
GACCGAGATCCGCTACGGCGCGCTCGCGATCGGCTTCAACGTGTCGGTGTCGCTGTTCGGCGGCACGACGCCGCTCGCCG
CCGCGTGGCTCGTCGACGCGACGGGCAACCTGATGATGCCCGCGTACTACCTGATGGGCGCGGCCGTGATCGGCGCGATC
TCGGTGCTCGCGCTGCCCGAGAGCGCGCGCCAGCCGCTCAAGGGCTCGCCGCCCGCCGTCGCGTCGCACCGCGAGGCACA
CGCGCTCGCGCGCGAGATCAAGCGCCGCGAGGCGGCCGAGCGCGACGACAGCGGCTACCCGTCGGCCGCGGCGTTGCGCG
CGTGA

Upstream 100 bases:

>100_bases
TCGCCGACCGTCCCCATGCGGCGGTCTTTTCTCACGAGTGCAAGCGCGCGACGACCCTCGGAGCCGCGCCGATCGGTTTC
CGTTATGGATTAAGCGTTTC

Downstream 100 bases:

>100_bases
GGTTCGGCCGCCGCGACGCCGCCGTGTAGGCGCGTCGCTCGATGACGGACCGCGGAGGCGGTCTCGCGATGCCCGTCGGG
CGTGGCGAGACCGCCTCCGC

Product: putative proline/betaine transporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Proline porter II; PPII [H]

Number of amino acids: Translated: 534; Mature: 534

Protein sequence:

>534_residues
MRPFTLATDPRPIASGTPGKPWRTAPRRAVRPPSQEFSTLTATPAPSSSSSAPTEGALPAAAHEITVVDQGLLKRAVGAM
ALGNAMEWFDFGVYSYIAVTLGQVFFPSSSPSAQLLATFGTFAAAFLVRPLGGMVFGPLGDRIGRQRVLAMTMIMMAVGT
FAIGLIPSYDSIGLLAPVLLLVARLVQGFSTGGEYGGAATFIAEFSTDKRRGFMGSFLEFGTLIGYVMGAGVVALLTASL
SHDALLSWGWRVPFLIAGPLGLIGLYIRMRLEETPAFKRQAEAREAQDKAVPKAHFRRQLARHWRALLLCVGLVLIFNVT
DYMALSYLPSYLSSTLHFDEAHGLVLILIVMVLMMPMTLATGRLSDAVGRKPVMLAGCVGLFALAIPALLLIRTGETALV
FGGLLILGALLSCFTGVMPSALPALFPTEIRYGALAIGFNVSVSLFGGTTPLAAAWLVDATGNLMMPAYYLMGAAVIGAI
SVLALPESARQPLKGSPPAVASHREAHALAREIKRREAAERDDSGYPSAAALRA

Sequences:

>Translated_534_residues
MRPFTLATDPRPIASGTPGKPWRTAPRRAVRPPSQEFSTLTATPAPSSSSSAPTEGALPAAAHEITVVDQGLLKRAVGAM
ALGNAMEWFDFGVYSYIAVTLGQVFFPSSSPSAQLLATFGTFAAAFLVRPLGGMVFGPLGDRIGRQRVLAMTMIMMAVGT
FAIGLIPSYDSIGLLAPVLLLVARLVQGFSTGGEYGGAATFIAEFSTDKRRGFMGSFLEFGTLIGYVMGAGVVALLTASL
SHDALLSWGWRVPFLIAGPLGLIGLYIRMRLEETPAFKRQAEAREAQDKAVPKAHFRRQLARHWRALLLCVGLVLIFNVT
DYMALSYLPSYLSSTLHFDEAHGLVLILIVMVLMMPMTLATGRLSDAVGRKPVMLAGCVGLFALAIPALLLIRTGETALV
FGGLLILGALLSCFTGVMPSALPALFPTEIRYGALAIGFNVSVSLFGGTTPLAAAWLVDATGNLMMPAYYLMGAAVIGAI
SVLALPESARQPLKGSPPAVASHREAHALAREIKRREAAERDDSGYPSAAALRA
>Mature_534_residues
MRPFTLATDPRPIASGTPGKPWRTAPRRAVRPPSQEFSTLTATPAPSSSSSAPTEGALPAAAHEITVVDQGLLKRAVGAM
ALGNAMEWFDFGVYSYIAVTLGQVFFPSSSPSAQLLATFGTFAAAFLVRPLGGMVFGPLGDRIGRQRVLAMTMIMMAVGT
FAIGLIPSYDSIGLLAPVLLLVARLVQGFSTGGEYGGAATFIAEFSTDKRRGFMGSFLEFGTLIGYVMGAGVVALLTASL
SHDALLSWGWRVPFLIAGPLGLIGLYIRMRLEETPAFKRQAEAREAQDKAVPKAHFRRQLARHWRALLLCVGLVLIFNVT
DYMALSYLPSYLSSTLHFDEAHGLVLILIVMVLMMPMTLATGRLSDAVGRKPVMLAGCVGLFALAIPALLLIRTGETALV
FGGLLILGALLSCFTGVMPSALPALFPTEIRYGALAIGFNVSVSLFGGTTPLAAAWLVDATGNLMMPAYYLMGAAVIGAI
SVLALPESARQPLKGSPPAVASHREAHALAREIKRREAAERDDSGYPSAAALRA

Specific function: Proton symporter that senses osmotic shifts and responds by importing osmolytes such as proline, glycine betaine, stachydrine, pipecolic acid, ectoine and taurine. It is both an osmosensor and an osmoregulator which is available to participate early in th

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=475, Percent_Identity=51.3684210526316, Blast_Score=517, Evalue=1e-148,
Organism=Escherichia coli, GI1788292, Length=444, Percent_Identity=31.5315315315315, Blast_Score=206, Evalue=4e-54,
Organism=Escherichia coli, GI1788942, Length=382, Percent_Identity=34.8167539267016, Blast_Score=199, Evalue=3e-52,
Organism=Escherichia coli, GI1789941, Length=439, Percent_Identity=34.1685649202733, Blast_Score=184, Evalue=1e-47,
Organism=Escherichia coli, GI87082231, Length=210, Percent_Identity=28.5714285714286, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI87082404, Length=365, Percent_Identity=24.9315068493151, Blast_Score=75, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR015041
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF08946 Osmo_CC; PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 56556; Mature: 56556

Theoretical pI: Translated: 9.90; Mature: 9.90

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1 ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRPFTLATDPRPIASGTPGKPWRTAPRRAVRPPSQEFSTLTATPAPSSSSSAPTEGALPA
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHCCEEECCCCCCCCCCCCCCCCCCC
AAHEITVVDQGLLKRAVGAMALGNAMEWFDFGVYSYIAVTLGQVFFPSSSPSAQLLATFG
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHH
TFAAAFLVRPLGGMVFGPLGDRIGRQRVLAMTMIMMAVGTFAIGLIPSYDSIGLLAPVLL
HHHHHHHHHHHCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHH
LVARLVQGFSTGGEYGGAATFIAEFSTDKRRGFMGSFLEFGTLIGYVMGAGVVALLTASL
HHHHHHHHHCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
SHDALLSWGWRVPFLIAGPLGLIGLYIRMRLEETPAFKRQAEAREAQDKAVPKAHFRRQL
CHHHHHHCCCCCCCCEECHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCHHHHHHHH
ARHWRALLLCVGLVLIFNVTDYMALSYLPSYLSSTLHFDEAHGLVLILIVMVLMMPMTLA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCHHHHHHHHHHHHHHHHH
TGRLSDAVGRKPVMLAGCVGLFALAIPALLLIRTGETALVFGGLLILGALLSCFTGVMPS
HCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
ALPALFPTEIRYGALAIGFNVSVSLFGGTTPLAAAWLVDATGNLMMPAYYLMGAAVIGAI
HHHHHCCCHHCCCEEEEECEEEEEEECCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
SVLALPESARQPLKGSPPAVASHREAHALAREIKRREAAERDDSGYPSAAALRA
HHHHCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCC
>Mature Secondary Structure
MRPFTLATDPRPIASGTPGKPWRTAPRRAVRPPSQEFSTLTATPAPSSSSSAPTEGALPA
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHCCEEECCCCCCCCCCCCCCCCCCC
AAHEITVVDQGLLKRAVGAMALGNAMEWFDFGVYSYIAVTLGQVFFPSSSPSAQLLATFG
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHH
TFAAAFLVRPLGGMVFGPLGDRIGRQRVLAMTMIMMAVGTFAIGLIPSYDSIGLLAPVLL
HHHHHHHHHHHCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHH
LVARLVQGFSTGGEYGGAATFIAEFSTDKRRGFMGSFLEFGTLIGYVMGAGVVALLTASL
HHHHHHHHHCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
SHDALLSWGWRVPFLIAGPLGLIGLYIRMRLEETPAFKRQAEAREAQDKAVPKAHFRRQL
CHHHHHHCCCCCCCCEECHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCHHHHHHHH
ARHWRALLLCVGLVLIFNVTDYMALSYLPSYLSSTLHFDEAHGLVLILIVMVLMMPMTLA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCHHHHHHHHHHHHHHHHH
TGRLSDAVGRKPVMLAGCVGLFALAIPALLLIRTGETALVFGGLLILGALLSCFTGVMPS
HCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
ALPALFPTEIRYGALAIGFNVSVSLFGGTTPLAAAWLVDATGNLMMPAYYLMGAAVIGAI
HHHHHCCCHHCCCEEEEECEEEEEEECCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
SVLALPESARQPLKGSPPAVASHREAHALAREIKRREAAERDDSGYPSAAALRA
HHHHCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]