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The map label for this gene is purT [H]

Identifier: 126452032

GI number: 126452032

Start: 1177996

End: 1179210

Strand: Direct

Name: purT [H]

Synonym: BURPS1106A_1196

Alternate gene names: 126452032

Gene position: 1177996-1179210 (Clockwise)

Preceding gene: 126454939

Following gene: 126451713

Centisome position: 29.54

GC content: 71.93

Gene sequence:

>1215_bases
ATGCAGATCGGTCAGCGGCTCGGCACGCCGCTTTCGCCGTCCGCCACGCGCGTCATGCTGCTCGGCGCGGGCGAGTTGGG
CAAGGAAGTCATCATCGCGTTGCAGCGGCTCGGCGTCGAAGTGATCGCCGTCGACCGCTATCCGAACGCACCCGGCCATC
AGGTCGCGCATCGCGCGCACGTGATCGACATGACGGACCCGGACGCGTTGCGCGCGCTCGTCGACGCGGAGCGTCCGCAT
CTCGTCGTGCCCGAAATCGAGGCGATCGCGACCGACGCGCTCGCCGCGATCGAGGCGGCCGGCGTCTGCGAGGTGATCCC
GACCGCGCGCGCGACGCAGCTCACGATGAATCGCGAGGGCATTCGCCGGCTCGCGGCCGAGGAGCTCGGGCTGCCGACGT
CGCCGTACGCGTTCGCGCAGTCGTTCGACGAATTCGCGGCGGCCGTCGCGCGGATCGGCTTTCCGTGCGTCGTGAAGCCC
GTGATGTCGTCGTCGGGCAAGGGGCAGTCGGTCGTGAGAAGCGAGGCCGACATCGAGCCCGCGTGGCGCTACGCGATGGC
GGGCGGCCGCGTGAATCACGGCCGCGTGATCGTCGAGGGCTTCATCCGGTTCGATTACGAGATCACGCAGCTCACGGTGC
GCGCGATCGATCCGGCGAGCGGGCAGACGCGCACCTCCTTCTGCGCGCCGATCGGGCACCTGCAGGTGGCGGGCGACTAC
GTCGAATCGTGGCAGCCGCAGCCGATGAGCGCGAAGGCGCTCGAGCGCTCGCGCGACATCGCGCATCGCGTGACGAGCGC
GCTCGGCGGCCGCGGGATCTTCGGCGTCGAGCTGTTCGTGCGCGGTGACGATGTCTGGTTCTCCGAGGTGAGCCCGCGCC
CGCACGACACGGGGCTCGTGACGCTCGCGTCGCAGCGCCAGTCGGAGTTCGAGCTGCATGCGCGCGCGATCCTCGGCCTG
CCGGTCGAGCCCGCGCTCGCGACGCCCGCCGCGTCGGCCGTGATCTACGGCGGGCTCGACGAAGCGGGCATCGCGTTCGA
GGGCGTGCGCGACGCGCTCGCGGTGCCGGGCGCCGATCTGCGGCTGTTCGGCAAGCCCGAGAGCTTCGCGAAGCGCCGGA
TGGGCGTCGCGCTCGCGACGGGCGCGAACGTCGACGAAGCGCGCGAGCGCGCGAAGCGGGCCGCCGCCGCGGTGCGTCCG
GTGTCCGCGCGTTGA

Upstream 100 bases:

>100_bases
CCGCGCGCGCCGGCGCGCGCCCAATCGGCCGGCGGCGCGCGGGCCCTGCGTGTTAGCATCGCAGGTCGTTTTCGCCATCC
ATCATCCAGCGGGAAATCTC

Downstream 100 bases:

>100_bases
CGAGTGCTGACGAGTGCTGACGTGTGGCGCGCATCGCGCGCCTGCGCATGCCGGGGCGGGGCGCGCCGGCCGCGACTGGC
CGCCGCGGTCGCGGCAATCG

Product: phosphoribosylglycinamide formyltransferase 2

Products: 5'-Phosphoribosyl-N-Formylglycinamide; Pyrophosphate; acetylphosphate; ADP [C]

Alternate protein names: GART 2; 5'-phosphoribosylglycinamide transformylase 2; Formate-dependent GAR transformylase; GAR transformylase 2 [H]

Number of amino acids: Translated: 404; Mature: 404

Protein sequence:

>404_residues
MQIGQRLGTPLSPSATRVMLLGAGELGKEVIIALQRLGVEVIAVDRYPNAPGHQVAHRAHVIDMTDPDALRALVDAERPH
LVVPEIEAIATDALAAIEAAGVCEVIPTARATQLTMNREGIRRLAAEELGLPTSPYAFAQSFDEFAAAVARIGFPCVVKP
VMSSSGKGQSVVRSEADIEPAWRYAMAGGRVNHGRVIVEGFIRFDYEITQLTVRAIDPASGQTRTSFCAPIGHLQVAGDY
VESWQPQPMSAKALERSRDIAHRVTSALGGRGIFGVELFVRGDDVWFSEVSPRPHDTGLVTLASQRQSEFELHARAILGL
PVEPALATPAASAVIYGGLDEAGIAFEGVRDALAVPGADLRLFGKPESFAKRRMGVALATGANVDEARERAKRAAAAVRP
VSAR

Sequences:

>Translated_404_residues
MQIGQRLGTPLSPSATRVMLLGAGELGKEVIIALQRLGVEVIAVDRYPNAPGHQVAHRAHVIDMTDPDALRALVDAERPH
LVVPEIEAIATDALAAIEAAGVCEVIPTARATQLTMNREGIRRLAAEELGLPTSPYAFAQSFDEFAAAVARIGFPCVVKP
VMSSSGKGQSVVRSEADIEPAWRYAMAGGRVNHGRVIVEGFIRFDYEITQLTVRAIDPASGQTRTSFCAPIGHLQVAGDY
VESWQPQPMSAKALERSRDIAHRVTSALGGRGIFGVELFVRGDDVWFSEVSPRPHDTGLVTLASQRQSEFELHARAILGL
PVEPALATPAASAVIYGGLDEAGIAFEGVRDALAVPGADLRLFGKPESFAKRRMGVALATGANVDEARERAKRAAAAVRP
VSAR
>Mature_404_residues
MQIGQRLGTPLSPSATRVMLLGAGELGKEVIIALQRLGVEVIAVDRYPNAPGHQVAHRAHVIDMTDPDALRALVDAERPH
LVVPEIEAIATDALAAIEAAGVCEVIPTARATQLTMNREGIRRLAAEELGLPTSPYAFAQSFDEFAAAVARIGFPCVVKP
VMSSSGKGQSVVRSEADIEPAWRYAMAGGRVNHGRVIVEGFIRFDYEITQLTVRAIDPASGQTRTSFCAPIGHLQVAGDY
VESWQPQPMSAKALERSRDIAHRVTSALGGRGIFGVELFVRGDDVWFSEVSPRPHDTGLVTLASQRQSEFELHARAILGL
PVEPALATPAASAVIYGGLDEAGIAFEGVRDALAVPGADLRLFGKPESFAKRRMGVALATGANVDEARERAKRAAAAVRP
VSAR

Specific function: Catalyzes two reactions:the first one is the production of beta-formyl glycinamide ribonucleotide (GAR) from formate, ATP and beta GAR; the second, a side reaction, is the production of acetyl phosphate and ADP from acetate and ATP [H]

COG id: COG0027

COG function: function code F; Formate-dependent phosphoribosylglycinamide formyltransferase (GAR transformylase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1788155, Length=396, Percent_Identity=63.1313131313131, Blast_Score=463, Evalue=1e-132,
Organism=Escherichia coli, GI1786733, Length=355, Percent_Identity=25.3521126760563, Blast_Score=64, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6324702, Length=385, Percent_Identity=23.6363636363636, Blast_Score=92, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR003135
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR005862
- InterPro:   IPR011054 [H]

Pfam domain/function: PF02222 ATP-grasp [H]

EC number: 2.1.2.- [C]

Molecular weight: Translated: 43084; Mature: 43084

Theoretical pI: Translated: 6.61; Mature: 6.61

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQIGQRLGTPLSPSATRVMLLGAGELGKEVIIALQRLGVEVIAVDRYPNAPGHQVAHRAH
CCCCHHCCCCCCCCCCEEEEEECCHHHHHHHHHHHHCCEEEEEEECCCCCCCHHHHHHEE
VIDMTDPDALRALVDAERPHLVVPEIEAIATDALAAIEAAGVCEVIPTARATQLTMNREG
EEECCCHHHHHHHHCCCCCEEECCCHHHHHHHHHHHHHHCCCHHHCCCCCCEEEEECHHH
IRRLAAEELGLPTSPYAFAQSFDEFAAAVARIGFPCVVKPVMSSSGKGQSVVRSEADIEP
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEHHHHCCCCCCCHHHHCCCCCCH
AWRYAMAGGRVNHGRVIVEGFIRFDYEITQLTVRAIDPASGQTRTSFCAPIGHLQVAGDY
HHHHHHCCCCCCCCEEEEEEEHHCCEEEEEEEEEEECCCCCCCCHHHHCCCCCEEECCHH
VESWQPQPMSAKALERSRDIAHRVTSALGGRGIFGVELFVRGDDVWFSEVSPRPHDTGLV
HHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCEEEECCCCCCCCCCEE
TLASQRQSEFELHARAILGLPVEPALATPAASAVIYGGLDEAGIAFEGVRDALAVPGADL
EEECCCCHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCCCCCCCHHHHHHHHHCCCCCE
RLFGKPESFAKRRMGVALATGANVDEARERAKRAAAAVRPVSAR
EEECCCHHHHHHHCCEEEECCCCHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MQIGQRLGTPLSPSATRVMLLGAGELGKEVIIALQRLGVEVIAVDRYPNAPGHQVAHRAH
CCCCHHCCCCCCCCCCEEEEEECCHHHHHHHHHHHHCCEEEEEEECCCCCCCHHHHHHEE
VIDMTDPDALRALVDAERPHLVVPEIEAIATDALAAIEAAGVCEVIPTARATQLTMNREG
EEECCCHHHHHHHHCCCCCEEECCCHHHHHHHHHHHHHHCCCHHHCCCCCCEEEEECHHH
IRRLAAEELGLPTSPYAFAQSFDEFAAAVARIGFPCVVKPVMSSSGKGQSVVRSEADIEP
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEHHHHCCCCCCCHHHHCCCCCCH
AWRYAMAGGRVNHGRVIVEGFIRFDYEITQLTVRAIDPASGQTRTSFCAPIGHLQVAGDY
HHHHHHCCCCCCCCEEEEEEEHHCCEEEEEEEEEEECCCCCCCCHHHHCCCCCEEECCHH
VESWQPQPMSAKALERSRDIAHRVTSALGGRGIFGVELFVRGDDVWFSEVSPRPHDTGLV
HHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCEEEECCCCCCCCCCEE
TLASQRQSEFELHARAILGLPVEPALATPAASAVIYGGLDEAGIAFEGVRDALAVPGADL
EEECCCCHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCCCCCCCHHHHHHHHHCCCCCE
RLFGKPESFAKRRMGVALATGANVDEARERAKRAAAAVRPVSAR
EEECCCHHHHHHHCCEEEECCCCHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Formate; 5'-Phospho-Ribosylglycinamide; acetate; ATP [C]

Specific reaction: Formate + ATP + 5'-Phospho-Ribosylglycinamide = 5'-Phosphoribosyl-N-Formylglycinamide + ADP + Pyrophosphate. acetate + ATP = acetylphosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA