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The map label for this gene is potH [H]

Identifier: 126452007

GI number: 126452007

Start: 2161891

End: 2162820

Strand: Reverse

Name: potH [H]

Synonym: BURPS1106A_2177

Alternate gene names: 126452007

Gene position: 2162820-2161891 (Counterclockwise)

Preceding gene: 126453071

Following gene: 126455120

Centisome position: 54.23

GC content: 62.69

Gene sequence:

>930_bases
ATGAACGCGCTTTCGTCCCTGTTTGCGTGGCCCGTCAGGAAGTTCGGCCTCACAGGCAGAACGGCCGTGATCGCGGGCCC
GTTCTTCTGGCTCGTGCTGTTCTTCCTCGTGCCGTTCGTGCTCGTCGTCAAGATCAGTTTCGCGCAGCTGCAGCTCGGCA
TTCCGCCGTATACGGAACTCACGGCGCTCGCCGACGGCGTGCTCAGCATCAAGCTGAACCTGCAGCACTACGCGTTCCTG
TTCAGCGACAGCCTGTACTTCGCGACCTACGTGAACTCGGTCGTCGTCGCGGCCGTCACGACGCTGCTGTGCCTGCTGAT
CGGCTATCCGATGGCGTACTACATCGCGCGCTCGAACCCGGGCACGCGCAACCTGCTGATGATGGCGGTGATGCTGCCGT
TCTGGACGTCGTTCCTGATCCGCGTGTATGCATGGATCGGCATCCTGAAGAACAACGGGCTGCTCAACAACTTCCTGCTG
TGGACGGGGCTCATCAGCACGCCGATCGAGCTGTACCGGACGAACGTCGGTGTCTATATCGGGATGGTCTACTCGTATCT
GCCGTTCCTCGTGATGCCGCTCTACGCGCACCTGGTGAAGATGGACCTGCGCCTGCTCGAAGCCGCCTACGACCTGGGCG
CGAAGCCGTGGAAGGCGTTCGTGCAGATCACGCTGCCGCTGTCGAAGAACGGGATCATCGCGGGCTGCCTGCTCGTGTTC
ATCCCGGCCGTCGGCGAGTACGTGATTCCCGAGCTGCTTGGCGGCGCGAACACGCTGATGATCGGCCGCGTGATGTGGAA
CGAGTTCTTCAACAACGCGGACTGGCCGATGGCGTCGGCGGTGACCTGCGCGATGGTGCTGCTGCTCCTCGTGCCGATGG
CGATGTTCCAGCACTTCCAGGCGAAGGAGCAGGAGGGCCACCGCCGATGA

Upstream 100 bases:

>100_bases
CGAACGTGTCGAGCCTCGCGATCGCCGATCTCGATTCGCCCGGCTGGGGCGATGAAATCTACGTGCGCTGGAGCGCCGCG
GCCGGCGTGGTGCTGACGTC

Downstream 100 bases:

>100_bases
AGCCGAATCGCTATCTGCAGGTGCTGATGCTCGCGATCGGCTTCGGTTTCCTCTATATCCCGATCGCGAGCCTCGTCGTC
TATTCGTTCAACGATTCGAA

Product: putrescine ABC transporter, permease protein PotH

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 309; Mature: 309

Protein sequence:

>309_residues
MNALSSLFAWPVRKFGLTGRTAVIAGPFFWLVLFFLVPFVLVVKISFAQLQLGIPPYTELTALADGVLSIKLNLQHYAFL
FSDSLYFATYVNSVVVAAVTTLLCLLIGYPMAYYIARSNPGTRNLLMMAVMLPFWTSFLIRVYAWIGILKNNGLLNNFLL
WTGLISTPIELYRTNVGVYIGMVYSYLPFLVMPLYAHLVKMDLRLLEAAYDLGAKPWKAFVQITLPLSKNGIIAGCLLVF
IPAVGEYVIPELLGGANTLMIGRVMWNEFFNNADWPMASAVTCAMVLLLLVPMAMFQHFQAKEQEGHRR

Sequences:

>Translated_309_residues
MNALSSLFAWPVRKFGLTGRTAVIAGPFFWLVLFFLVPFVLVVKISFAQLQLGIPPYTELTALADGVLSIKLNLQHYAFL
FSDSLYFATYVNSVVVAAVTTLLCLLIGYPMAYYIARSNPGTRNLLMMAVMLPFWTSFLIRVYAWIGILKNNGLLNNFLL
WTGLISTPIELYRTNVGVYIGMVYSYLPFLVMPLYAHLVKMDLRLLEAAYDLGAKPWKAFVQITLPLSKNGIIAGCLLVF
IPAVGEYVIPELLGGANTLMIGRVMWNEFFNNADWPMASAVTCAMVLLLLVPMAMFQHFQAKEQEGHRR
>Mature_309_residues
MNALSSLFAWPVRKFGLTGRTAVIAGPFFWLVLFFLVPFVLVVKISFAQLQLGIPPYTELTALADGVLSIKLNLQHYAFL
FSDSLYFATYVNSVVVAAVTTLLCLLIGYPMAYYIARSNPGTRNLLMMAVMLPFWTSFLIRVYAWIGILKNNGLLNNFLL
WTGLISTPIELYRTNVGVYIGMVYSYLPFLVMPLYAHLVKMDLRLLEAAYDLGAKPWKAFVQITLPLSKNGIIAGCLLVF
IPAVGEYVIPELLGGANTLMIGRVMWNEFFNNADWPMASAVTCAMVLLLLVPMAMFQHFQAKEQEGHRR

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine [H]

COG id: COG1176

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component I

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787080, Length=289, Percent_Identity=59.1695501730104, Blast_Score=333, Evalue=7e-93,
Organism=Escherichia coli, GI1787369, Length=266, Percent_Identity=42.4812030075188, Blast_Score=194, Evalue=4e-51,
Organism=Escherichia coli, GI1787713, Length=245, Percent_Identity=28.9795918367347, Blast_Score=111, Evalue=6e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 34646; Mature: 34646

Theoretical pI: Translated: 9.34; Mature: 9.34

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNALSSLFAWPVRKFGLTGRTAVIAGPFFWLVLFFLVPFVLVVKISFAQLQLGIPPYTEL
CCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCHHHH
TALADGVLSIKLNLQHYAFLFSDSLYFATYVNSVVVAAVTTLLCLLIGYPMAYYIARSNP
HHHHCCCEEEEEEHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHEEECCCC
GTRNLLMMAVMLPFWTSFLIRVYAWIGILKNNGLLNNFLLWTGLISTPIELYRTNVGVYI
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
GMVYSYLPFLVMPLYAHLVKMDLRLLEAAYDLGAKPWKAFVQITLPLSKNGIIAGCLLVF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCHHHHHHHHH
IPAVGEYVIPELLGGANTLMIGRVMWNEFFNNADWPMASAVTCAMVLLLLVPMAMFQHFQ
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
AKEQEGHRR
HHHHCCCCC
>Mature Secondary Structure
MNALSSLFAWPVRKFGLTGRTAVIAGPFFWLVLFFLVPFVLVVKISFAQLQLGIPPYTEL
CCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCHHHH
TALADGVLSIKLNLQHYAFLFSDSLYFATYVNSVVVAAVTTLLCLLIGYPMAYYIARSNP
HHHHCCCEEEEEEHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHEEECCCC
GTRNLLMMAVMLPFWTSFLIRVYAWIGILKNNGLLNNFLLWTGLISTPIELYRTNVGVYI
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
GMVYSYLPFLVMPLYAHLVKMDLRLLEAAYDLGAKPWKAFVQITLPLSKNGIIAGCLLVF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCHHHHHHHHH
IPAVGEYVIPELLGGANTLMIGRVMWNEFFNNADWPMASAVTCAMVLLLLVPMAMFQHFQ
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
AKEQEGHRR
HHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8416922; 8905232; 9278503 [H]