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The map label for this gene is cca [H]

Identifier: 126451805

GI number: 126451805

Start: 255843

End: 257084

Strand: Direct

Name: cca [H]

Synonym: BURPS1106A_0272

Alternate gene names: 126451805

Gene position: 255843-257084 (Clockwise)

Preceding gene: 126454638

Following gene: 126451866

Centisome position: 6.41

GC content: 72.62

Gene sequence:

>1242_bases
ATGAAGATCTACGCGGTGGGCGGCGCGATCCGCGACGCACTGCTCGGCCTGCCGGTGCGCGACCGCGATTACGTCGTCGT
CGGCGCGACGCCCGAGCAGATGGCCGCGCAGCGCTTCAGGCCGGTCGGCAAGGATTTTCCGGTGTTCCTGCATCCGGATA
CGCACGAGGAATACGCGCTCGCGCGCACCGAACGCAAGACGGCGGCCGGCTATCACGGCTTCCAGTTCTATTACGCGCCC
GACGTCACGCTCGAGCAGGATCTCGTGCGGCGCGACCTGACGATCAACGCGATGGCGCGCGAAGTGAGCCCGGACGGCGC
GCTCGTCGGGCCGGTCGTCGATCCGTTCGGCGGGCAGGCGGATTTGCGCGCGAAGCTGTTCCGGCACGTCGGCGACGCGT
TCGTCGAGGATCCGGTGCGCATCCTGCGCGTCGCGCGCTTCGCCGCGCGCTTCGCCGAGTTCGCCGTCGCGCCCGACACG
GCCGCGCTGATGCGCGCGATGGTCGACGCGGGCGAGGTCGACGCGCTCGTGCCCGAGCGCGTGTGGCAGGAGCTCGCGCG
CGGGCTGATGGAGGCGAAGCCGTCGCGGATGTTCGCGGTGCTGCGCGAGTGCGGCGCGCTCGCACGGATCCTGCCCGAGA
TCGACGCGCTCTTCGGCGTGCCGCAGCGCGCCGACTATCACCCGGAGGTCGACACGGGCGTGCACGTGATGATGGTGATC
GATCACGCGGCGAAGCAGGGCTACTCGCTGGCGGTGCGTTTCGCGGCGCTCACGCACGACCTCGGCAAGGCGACGACGCC
CGCCGACGTGCTGCCGCGTCACATCGGCCACGAGGGGCGCAGCGTCGATCTGCTGAAGCCGCTGTGCGAGCGGCTGCGGG
TGCCGAACGAGTGCCGCGATCTGGCGCTCGTCGTCGCGCGCGAGCACGGCAATCTGCATCGCGTGATGGAGATGGGCGCG
GCCGCGCTGGTGCGCCTGCTCGAGCGCGCGGATGCGCTGCGCAAGCCGGCGCGCTTCGCCGAGGCGCTGCAGGCGAGCGA
GGCCGACGCGCGCGGCCGGCTCGGCCTCGAGACGAAGCCCTATCCGCAGGCCGAACGGCTGCGGCAGGCGCTCGTCGCCG
CGCGCGCGGTCGATGCGGGCGCGATCGCGCAGGGGCTCGCGGGCGAGCCCGCGAAGATCAAGGATGCGGTGCACCGGGCG
CGCGTGCGCGCGGTCGCGCAGGCGGTCGGCGTGGCCGATTGA

Upstream 100 bases:

>100_bases
GCGCGTACGCGGCGCGGGTCGCCGAGCTGCCGGCCGTGCGCGAATGGATCGACGGCGCGCGCGGCGAGACGCGCGTGATC
GACATCTACGGGCCATCGCG

Downstream 100 bases:

>100_bases
CCTCCCCGGGCGGCATCGGCTGCGGGTGCGCCGCGTGAGCGCGCGCGGGCGATAAGGGCACGGCTCGCCGCGTATCTTGG
CGGTCGACGGTCGACGGTCA

Product: multifunctional tRNA nucleotidyl transferase/2'3'-cyclic phosphodiesterase/2'nucleotidase/phosphatase

Products: NA

Alternate protein names: CCA-adding enzyme; tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl-transferase; tRNA nucleotidyltransferase; tRNA-NT; 2'-nucleotidase; 2',3'-cyclic phosphodiesterase; Phosphatase [H]

Number of amino acids: Translated: 413; Mature: 413

Protein sequence:

>413_residues
MKIYAVGGAIRDALLGLPVRDRDYVVVGATPEQMAAQRFRPVGKDFPVFLHPDTHEEYALARTERKTAAGYHGFQFYYAP
DVTLEQDLVRRDLTINAMAREVSPDGALVGPVVDPFGGQADLRAKLFRHVGDAFVEDPVRILRVARFAARFAEFAVAPDT
AALMRAMVDAGEVDALVPERVWQELARGLMEAKPSRMFAVLRECGALARILPEIDALFGVPQRADYHPEVDTGVHVMMVI
DHAAKQGYSLAVRFAALTHDLGKATTPADVLPRHIGHEGRSVDLLKPLCERLRVPNECRDLALVVAREHGNLHRVMEMGA
AALVRLLERADALRKPARFAEALQASEADARGRLGLETKPYPQAERLRQALVAARAVDAGAIAQGLAGEPAKIKDAVHRA
RVRAVAQAVGVAD

Sequences:

>Translated_413_residues
MKIYAVGGAIRDALLGLPVRDRDYVVVGATPEQMAAQRFRPVGKDFPVFLHPDTHEEYALARTERKTAAGYHGFQFYYAP
DVTLEQDLVRRDLTINAMAREVSPDGALVGPVVDPFGGQADLRAKLFRHVGDAFVEDPVRILRVARFAARFAEFAVAPDT
AALMRAMVDAGEVDALVPERVWQELARGLMEAKPSRMFAVLRECGALARILPEIDALFGVPQRADYHPEVDTGVHVMMVI
DHAAKQGYSLAVRFAALTHDLGKATTPADVLPRHIGHEGRSVDLLKPLCERLRVPNECRDLALVVAREHGNLHRVMEMGA
AALVRLLERADALRKPARFAEALQASEADARGRLGLETKPYPQAERLRQALVAARAVDAGAIAQGLAGEPAKIKDAVHRA
RVRAVAQAVGVAD
>Mature_413_residues
MKIYAVGGAIRDALLGLPVRDRDYVVVGATPEQMAAQRFRPVGKDFPVFLHPDTHEEYALARTERKTAAGYHGFQFYYAP
DVTLEQDLVRRDLTINAMAREVSPDGALVGPVVDPFGGQADLRAKLFRHVGDAFVEDPVRILRVARFAARFAEFAVAPDT
AALMRAMVDAGEVDALVPERVWQELARGLMEAKPSRMFAVLRECGALARILPEIDALFGVPQRADYHPEVDTGVHVMMVI
DHAAKQGYSLAVRFAALTHDLGKATTPADVLPRHIGHEGRSVDLLKPLCERLRVPNECRDLALVVAREHGNLHRVMEMGA
AALVRLLERADALRKPARFAEALQASEADARGRLGLETKPYPQAERLRQALVAARAVDAGAIAQGLAGEPAKIKDAVHRA
RVRAVAQAVGVAD

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789436, Length=410, Percent_Identity=53.1707317073171, Blast_Score=423, Evalue=1e-120,
Organism=Escherichia coli, GI87081691, Length=232, Percent_Identity=29.7413793103448, Blast_Score=70, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012006
- InterPro:   IPR003607
- InterPro:   IPR002646 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.21; =2.7.7.25 [H]

Molecular weight: Translated: 45088; Mature: 45088

Theoretical pI: Translated: 7.78; Mature: 7.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIYAVGGAIRDALLGLPVRDRDYVVVGATPEQMAAQRFRPVGKDFPVFLHPDTHEEYAL
CEEEEECHHHHHHHHCCCCCCCCEEEECCCHHHHHHHHCCCCCCCCCEEECCCCCHHHHH
ARTERKTAAGYHGFQFYYAPDVTLEQDLVRRDLTINAMAREVSPDGALVGPVVDPFGGQA
HHHHHHHHCCCCCEEEEECCCCCHHHHHHHHCCHHHHHHHHCCCCCCEECCCCCCCCCCH
DLRAKLFRHVGDAFVEDPVRILRVARFAARFAEFAVAPDTAALMRAMVDAGEVDALVPER
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHHH
VWQELARGLMEAKPSRMFAVLRECGALARILPEIDALFGVPQRADYHPEVDTGVHVMMVI
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEHEE
DHAAKQGYSLAVRFAALTHDLGKATTPADVLPRHIGHEGRSVDLLKPLCERLRVPNECRD
HHHHHCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHCCCCHHHHH
LALVVAREHGNLHRVMEMGAAALVRLLERADALRKPARFAEALQASEADARGRLGLETKP
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
YPQAERLRQALVAARAVDAGAIAQGLAGEPAKIKDAVHRARVRAVAQAVGVAD
CCHHHHHHHHHHHHHHHCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKIYAVGGAIRDALLGLPVRDRDYVVVGATPEQMAAQRFRPVGKDFPVFLHPDTHEEYAL
CEEEEECHHHHHHHHCCCCCCCCEEEECCCHHHHHHHHCCCCCCCCCEEECCCCCHHHHH
ARTERKTAAGYHGFQFYYAPDVTLEQDLVRRDLTINAMAREVSPDGALVGPVVDPFGGQA
HHHHHHHHCCCCCEEEEECCCCCHHHHHHHHCCHHHHHHHHCCCCCCEECCCCCCCCCCH
DLRAKLFRHVGDAFVEDPVRILRVARFAARFAEFAVAPDTAALMRAMVDAGEVDALVPER
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHHH
VWQELARGLMEAKPSRMFAVLRECGALARILPEIDALFGVPQRADYHPEVDTGVHVMMVI
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEHEE
DHAAKQGYSLAVRFAALTHDLGKATTPADVLPRHIGHEGRSVDLLKPLCERLRVPNECRD
HHHHHCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHCCCCHHHHH
LALVVAREHGNLHRVMEMGAAALVRLLERADALRKPARFAEALQASEADARGRLGLETKP
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
YPQAERLRQALVAARAVDAGAIAQGLAGEPAKIKDAVHRARVRAVAQAVGVAD
CCHHHHHHHHHHHHHHHCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA