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The map label for this gene is narK [H]

Identifier: 126451711

GI number: 126451711

Start: 2640444

End: 2641724

Strand: Direct

Name: narK [H]

Synonym: BURPS1106A_2678

Alternate gene names: 126451711

Gene position: 2640444-2641724 (Clockwise)

Preceding gene: 126452085

Following gene: 126454415

Centisome position: 66.2

GC content: 68.07

Gene sequence:

>1281_bases
ATGGCTGCAACCTCCGCTTCCCCCGCCTCGCTCGACAAGCGCGCGATGCCCGTGCTCGCGTCGAGCACGATTGCATTCAC
GCTGTGCTTCGCCGTATGGATGATGTTCGCGATCCTCGGCATTCCGCTGAAGAAAACCCTCGGCCTGAACGACACCGAAT
TCGGCCTCATCGCCGCGATGCCCGTGCTCACCGGCTCGCTGATCCGCGTGCCGCTCGGCATCTGGACCGACCGCTACGGC
GGACGCATCGTGTTCTTCATCCTGATGCTCGTCACCGTCGTGCCGATCTGGCTGATCTCGTACGCGACCGAGCTCTGGCA
GTTCCTCCTGCTCGGCCTGTTCGTCGGGCTCGCGGGCGGCTCGTTCTCGGTCGGCACGCCCTACGTCGCGCGCTGGTTCC
CGAAAGCGCGCCAGGGTCTCGCGATGGGCGTGTTCGGCGCCGGCAACTCGGGCGCGGCCGTCAACAAGTTCGTCGCGCCC
GCGCTGATCGCCGCGGCGGGCACATGGACCATCGTGCCGCGCGTCTACGCGGTCGCGATGCTCGCGATGGCGCTCCTCTT
CTGGCTGTTCTCCGCCACCGATCCCGCGCACCGCTCGACGAACGCGACATCGCTGCGCGCGCAACTGCGCGTGCTGAAGA
ACCCGCGCGTATGGCGCTACTCGCAGTACTACTCGGTCGTGTTCGGCGGCTACGTCGGGCTGTCGCTGTGGCTCACGCAG
TACTACGTCGGCGAATACGGCTTCGGCATCCAGTCGGCCGCGTTCCTCGCCGCGTGCTTCTCGCTGCCGGGCGGCGTGCT
GCGCGCGATCGGCGGGTGGCTTTCCGACCGCTACGGCGCGTATCGCACGACGTGGTGGGTGATGTGGGTCTGCTGGGTGA
TGTTCTTCCTGCTCAGCTATCCGCCGACCGATTTCACGATCCGCGCCGCACACGGCCCGCTCGGCTTCCATCTGTCGCTC
ACGCCCGTCGCGTTCACCGCGCTGCTGTTCGCGGTCGGCATCGCGATGGCGGTCGGCAAGGCGTCGGTCTTCAAGTTCAT
CGCCGACGAGTTCCCGAACGACATCGGCGCGGTGTCGGGCGTCGTCGGCCTCGCGGGCGGCCTGGCCGGCTTCGCGCTGC
CGATTCTGTTCGGCGCGCTCGTCGACCTCACGGGCGTGCGCTCGACCTGCTTCATGCTGCTCTACGGCGCCGTCAGCGTG
AGCCTCGTCTGGATGTACTTCAGCTTCCGCGCCGAGCGTGCCGCGCACGACCAGCGCGTGCTCGCCACCCGCTCCGCGTG
A

Upstream 100 bases:

>100_bases
CGACCCAGCGACACGCGCGCGCCCGTTCCTCCGGCGAATAGGCAAGCGCATCGCCCGTCCCTACTCTTGAGATGCTCCTC
CTCGTCTCGAGAGAATCATC

Downstream 100 bases:

>100_bases
GCCTTGCTTCCTCGTCATCTTCGTTCACTCAACGGATTCGATCATGTCCACTTCTCTACTCGTGCGCTGGGATCCCGAAA
ACCCGGCCTTCTGGCAAGCC

Product: nitrate/nitrite porter (NNP) family protein

Products: NA

Alternate protein names: Nitrite facilitator [H]

Number of amino acids: Translated: 426; Mature: 425

Protein sequence:

>426_residues
MAATSASPASLDKRAMPVLASSTIAFTLCFAVWMMFAILGIPLKKTLGLNDTEFGLIAAMPVLTGSLIRVPLGIWTDRYG
GRIVFFILMLVTVVPIWLISYATELWQFLLLGLFVGLAGGSFSVGTPYVARWFPKARQGLAMGVFGAGNSGAAVNKFVAP
ALIAAAGTWTIVPRVYAVAMLAMALLFWLFSATDPAHRSTNATSLRAQLRVLKNPRVWRYSQYYSVVFGGYVGLSLWLTQ
YYVGEYGFGIQSAAFLAACFSLPGGVLRAIGGWLSDRYGAYRTTWWVMWVCWVMFFLLSYPPTDFTIRAAHGPLGFHLSL
TPVAFTALLFAVGIAMAVGKASVFKFIADEFPNDIGAVSGVVGLAGGLAGFALPILFGALVDLTGVRSTCFMLLYGAVSV
SLVWMYFSFRAERAAHDQRVLATRSA

Sequences:

>Translated_426_residues
MAATSASPASLDKRAMPVLASSTIAFTLCFAVWMMFAILGIPLKKTLGLNDTEFGLIAAMPVLTGSLIRVPLGIWTDRYG
GRIVFFILMLVTVVPIWLISYATELWQFLLLGLFVGLAGGSFSVGTPYVARWFPKARQGLAMGVFGAGNSGAAVNKFVAP
ALIAAAGTWTIVPRVYAVAMLAMALLFWLFSATDPAHRSTNATSLRAQLRVLKNPRVWRYSQYYSVVFGGYVGLSLWLTQ
YYVGEYGFGIQSAAFLAACFSLPGGVLRAIGGWLSDRYGAYRTTWWVMWVCWVMFFLLSYPPTDFTIRAAHGPLGFHLSL
TPVAFTALLFAVGIAMAVGKASVFKFIADEFPNDIGAVSGVVGLAGGLAGFALPILFGALVDLTGVRSTCFMLLYGAVSV
SLVWMYFSFRAERAAHDQRVLATRSA
>Mature_425_residues
AATSASPASLDKRAMPVLASSTIAFTLCFAVWMMFAILGIPLKKTLGLNDTEFGLIAAMPVLTGSLIRVPLGIWTDRYGG
RIVFFILMLVTVVPIWLISYATELWQFLLLGLFVGLAGGSFSVGTPYVARWFPKARQGLAMGVFGAGNSGAAVNKFVAPA
LIAAAGTWTIVPRVYAVAMLAMALLFWLFSATDPAHRSTNATSLRAQLRVLKNPRVWRYSQYYSVVFGGYVGLSLWLTQY
YVGEYGFGIQSAAFLAACFSLPGGVLRAIGGWLSDRYGAYRTTWWVMWVCWVMFFLLSYPPTDFTIRAAHGPLGFHLSLT
PVAFTALLFAVGIAMAVGKASVFKFIADEFPNDIGAVSGVVGLAGGLAGFALPILFGALVDLTGVRSTCFMLLYGAVSVS
LVWMYFSFRAERAAHDQRVLATRSA

Specific function: Involved in excretion of nitrite produced by the dissimilatory reduction of nitrate [H]

COG id: COG2223

COG function: function code P; Nitrate/nitrite transporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Nitrate/nitrite porter (TC 2.A.1.8) family [H]

Homologues:

Organism=Escherichia coli, GI1787475, Length=438, Percent_Identity=26.027397260274, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1787743, Length=431, Percent_Identity=25.9860788863109, Blast_Score=74, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 46056; Mature: 45925

Theoretical pI: Translated: 10.08; Mature: 10.08

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHCCCC
>Mature Secondary Structure 
AATSASPASLDKRAMPVLASSTIAFTLCFAVWMMFAILGIPLKKTLGLNDTEFGLIAAM
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCHHHHHHH
PVLTGSLIRVPLGIWTDRYGGRIVFFILMLVTVVPIWLISYATELWQFLLLGLFVGLAGG
HHHHCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
SFSVGTPYVARWFPKARQGLAMGVFGAGNSGAAVNKFVAPALIAAAGTWTIVPRVYAVAM
CCCCCCCHHHHHCHHHHCCCEEEEECCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHH
LAMALLFWLFSATDPAHRSTNATSLRAQLRVLKNPRVWRYSQYYSVVFGGYVGLSLWLTQ
HHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHH
YYVGEYGFGIQSAAFLAACFSLPGGVLRAIGGWLSDRYGAYRTTWWVMWVCWVMFFLLSY
HHHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCC
PPTDFTIRAAHGPLGFHLSLTPVAFTALLFAVGIAMAVGKASVFKFIADEFPNDIGAVSG
CCCCEEEEECCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
VVGLAGGLAGFALPILFGALVDLTGVRSTCFMLLYGAVSVSLVWMYFSFRAERAAHDQRV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LATRSA
HHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8846791; 9384377; 9353933 [H]