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The map label for this gene is ansA [H]

Identifier: 126451697

GI number: 126451697

Start: 2284987

End: 2286030

Strand: Reverse

Name: ansA [H]

Synonym: BURPS1106A_2305

Alternate gene names: 126451697

Gene position: 2286030-2284987 (Counterclockwise)

Preceding gene: 126454130

Following gene: 126453403

Centisome position: 57.32

GC content: 72.03

Gene sequence:

>1044_bases
ATGAACGCACCGACATCCTCCCATTCCCCTCACGTTTCGAACCCGGCGCCGCGCGCGCTGCCGCGCATCGCCGTCCTCGC
GACGGGCGGCACGATCGCGGGCGCGGCGCCCGACGCCGCGCAGACGGCCGGCTACCAGGCGGGCGCGCTCGGCGTCGACA
GGCTGCTCGCCGCCGTGCCCGCGCTCGCGCAGATCGCGACGATCGAGGCCGAGCAGGTCGCGAGCATCGACAGCAAGGAC
TTGTCGCCCGCGCTGTGGACGACGCTCGCCGAGCGGATCGACGCGCTTGCGGCGAACGACGCGATCGACGGCATCGTGAT
CACGCACGGCACCGACACGCTCGAAGAGACTGCGTACCTGCTGCATCTGACCGTGAAGACCGCGAAGCCTGTCGTGATGA
CGGCGGCGATGCGCCCGGCGACCGCGCTGTCGTCCGACGGCCCGCTCAACCTGCTGAACGCGGTCGCCGTCGCGGGCAGC
GCGGCGGCGCGCGGGCAGGGCGTGCTCGTCGCGTTCAACAACCGGATCCATAGCGCGCGCGACGTCGTGAAGACGAGCAC
CTATGCGGTCGATGCATTTCACTCGCCCGAACTGGGCGCGCTCGGCTGGGTGCAGGACGGCCGCATCGAGTTCGCGCGCC
GCGCGACGCGCCCGCATACGCTCGACTCGCGCTTCGCGATCGGCGCCGCCTGGCCGCAGGTCGAGGTCGTCGCGAGCTAT
GCGGGCGCATCGCGCGTGATCGTCGATGCATGCGTCGCGGCGGGCGCGCGCGGGCTCGTCGTCGCGGGCACGGGCAACGG
CTCGATCCATGCGGCGCTGCAGGCGGCGCTCGCCGATGCGGCGGCGAAGGGCGTCGCGATCGTGCGCGCGTCGCGCGTCG
GCTCGGGACACGTGATGCGCAACGGGGCGGCGAACGACGATGCGCTCGGCTTCGTGAGCGCGGGTTCGCTCAATCCGTAC
AAGGCGCGCGTGCTGCTGATGCTCGCGCTGGCGGCCGGCGTCGCCGACGTGCGCGAATTGCAGCAGGTGTTCGATACGTA
TTGA

Upstream 100 bases:

>100_bases
GTCGCGCAACCGGCGGGAGCGGCGCGCGCGAGCGCGAGCGTGCACCCGGGAACACACCGCGCTTGCCGAAGGGCGGCCGG
CTGCCGTTACAATCGCGGCC

Downstream 100 bases:

>100_bases
CCGAATCATGCGCGCCGGCATCGGAGGCGGCGCGGCCGGCCGATCGCGCGGCGTGCGCCGCCTCCGAGCGGACCGATGCG
CGCCGAATCGAACCGGAGAC

Product: L-asparaginase, type II

Products: NA

Alternate protein names: L-ASNase; L-asparagine amidohydrolase [H]

Number of amino acids: Translated: 347; Mature: 347

Protein sequence:

>347_residues
MNAPTSSHSPHVSNPAPRALPRIAVLATGGTIAGAAPDAAQTAGYQAGALGVDRLLAAVPALAQIATIEAEQVASIDSKD
LSPALWTTLAERIDALAANDAIDGIVITHGTDTLEETAYLLHLTVKTAKPVVMTAAMRPATALSSDGPLNLLNAVAVAGS
AAARGQGVLVAFNNRIHSARDVVKTSTYAVDAFHSPELGALGWVQDGRIEFARRATRPHTLDSRFAIGAAWPQVEVVASY
AGASRVIVDACVAAGARGLVVAGTGNGSIHAALQAALADAAAKGVAIVRASRVGSGHVMRNGAANDDALGFVSAGSLNPY
KARVLLMLALAAGVADVRELQQVFDTY

Sequences:

>Translated_347_residues
MNAPTSSHSPHVSNPAPRALPRIAVLATGGTIAGAAPDAAQTAGYQAGALGVDRLLAAVPALAQIATIEAEQVASIDSKD
LSPALWTTLAERIDALAANDAIDGIVITHGTDTLEETAYLLHLTVKTAKPVVMTAAMRPATALSSDGPLNLLNAVAVAGS
AAARGQGVLVAFNNRIHSARDVVKTSTYAVDAFHSPELGALGWVQDGRIEFARRATRPHTLDSRFAIGAAWPQVEVVASY
AGASRVIVDACVAAGARGLVVAGTGNGSIHAALQAALADAAAKGVAIVRASRVGSGHVMRNGAANDDALGFVSAGSLNPY
KARVLLMLALAAGVADVRELQQVFDTY
>Mature_347_residues
MNAPTSSHSPHVSNPAPRALPRIAVLATGGTIAGAAPDAAQTAGYQAGALGVDRLLAAVPALAQIATIEAEQVASIDSKD
LSPALWTTLAERIDALAANDAIDGIVITHGTDTLEETAYLLHLTVKTAKPVVMTAAMRPATALSSDGPLNLLNAVAVAGS
AAARGQGVLVAFNNRIHSARDVVKTSTYAVDAFHSPELGALGWVQDGRIEFARRATRPHTLDSRFAIGAAWPQVEVVASY
AGASRVIVDACVAAGARGLVVAGTGNGSIHAALQAALADAAAKGVAIVRASRVGSGHVMRNGAANDDALGFVSAGSLNPY
KARVLLMLALAAGVADVRELQQVFDTY

Specific function: Unknown

COG id: COG0252

COG function: function code EJ; L-asparaginase/archaeal Glu-tRNAGln amidotransferase subunit D

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the asparaginase 1 family [H]

Homologues:

Organism=Homo sapiens, GI122937331, Length=227, Percent_Identity=29.5154185022026, Blast_Score=72, Evalue=1e-12,
Organism=Escherichia coli, GI1789327, Length=331, Percent_Identity=47.1299093655589, Blast_Score=281, Evalue=6e-77,
Organism=Escherichia coli, GI1788065, Length=296, Percent_Identity=31.7567567567568, Blast_Score=107, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI17558278, Length=333, Percent_Identity=28.2282282282282, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17558280, Length=333, Percent_Identity=28.2282282282282, Blast_Score=83, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI193207372, Length=333, Percent_Identity=28.2282282282282, Blast_Score=83, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI193207374, Length=333, Percent_Identity=28.2282282282282, Blast_Score=83, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6323189, Length=335, Percent_Identity=37.3134328358209, Blast_Score=197, Evalue=2e-51,
Organism=Saccharomyces cerevisiae, GI6323187, Length=335, Percent_Identity=37.3134328358209, Blast_Score=197, Evalue=2e-51,
Organism=Saccharomyces cerevisiae, GI6323186, Length=335, Percent_Identity=37.3134328358209, Blast_Score=197, Evalue=2e-51,
Organism=Saccharomyces cerevisiae, GI6323184, Length=335, Percent_Identity=37.3134328358209, Blast_Score=197, Evalue=2e-51,
Organism=Saccharomyces cerevisiae, GI6320527, Length=338, Percent_Identity=34.6153846153846, Blast_Score=189, Evalue=8e-49,
Organism=Drosophila melanogaster, GI24639609, Length=254, Percent_Identity=27.9527559055118, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24645530, Length=237, Percent_Identity=28.6919831223629, Blast_Score=73, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004550
- InterPro:   IPR006034
- InterPro:   IPR020827 [H]

Pfam domain/function: PF00710 Asparaginase [H]

EC number: =3.5.1.1 [H]

Molecular weight: Translated: 35293; Mature: 35293

Theoretical pI: Translated: 7.03; Mature: 7.03

Prosite motif: PS00144 ASN_GLN_ASE_1 ; PS00917 ASN_GLN_ASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAPTSSHSPHVSNPAPRALPRIAVLATGGTIAGAAPDAAQTAGYQAGALGVDRLLAAVP
CCCCCCCCCCCCCCCCCCCCCEEEEEEECCEECCCCCCHHHHCCCCCCCHHHHHHHHHHH
ALAQIATIEAEQVASIDSKDLSPALWTTLAERIDALAANDAIDGIVITHGTDTLEETAYL
HHHHHHHHCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCHHHCEEE
LHLTVKTAKPVVMTAAMRPATALSSDGPLNLLNAVAVAGSAAARGQGVLVAFNNRIHSAR
EEEEECCCCCEEEEECCCCCHHCCCCCCHHHHHHHHHHCCCCCCCCEEEEEECCCHHHHH
DVVKTSTYAVDAFHSPELGALGWVQDGRIEFARRATRPHTLDSRFAIGAAWPQVEVVASY
HHHHHHHEEEECCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCEEECCCCCHHHHHHHH
AGASRVIVDACVAAGARGLVVAGTGNGSIHAALQAALADAAAKGVAIVRASRVGSGHVMR
CCCHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHCCEEEEEECCCCCCCEEE
NGAANDDALGFVSAGSLNPYKARVLLMLALAAGVADVRELQQVFDTY
CCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHCC
>Mature Secondary Structure
MNAPTSSHSPHVSNPAPRALPRIAVLATGGTIAGAAPDAAQTAGYQAGALGVDRLLAAVP
CCCCCCCCCCCCCCCCCCCCCEEEEEEECCEECCCCCCHHHHCCCCCCCHHHHHHHHHHH
ALAQIATIEAEQVASIDSKDLSPALWTTLAERIDALAANDAIDGIVITHGTDTLEETAYL
HHHHHHHHCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCHHHCEEE
LHLTVKTAKPVVMTAAMRPATALSSDGPLNLLNAVAVAGSAAARGQGVLVAFNNRIHSAR
EEEEECCCCCEEEEECCCCCHHCCCCCCHHHHHHHHHHCCCCCCCCEEEEEECCCHHHHH
DVVKTSTYAVDAFHSPELGALGWVQDGRIEFARRATRPHTLDSRFAIGAAWPQVEVVASY
HHHHHHHEEEECCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCEEECCCCCHHHHHHHH
AGASRVIVDACVAAGARGLVVAGTGNGSIHAALQAALADAAAKGVAIVRASRVGSGHVMR
CCCHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHCCEEEEEECCCCCCCEEE
NGAANDDALGFVSAGSLNPYKARVLLMLALAAGVADVRELQQVFDTY
CCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8898907; 14500908 [H]