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The map label for this gene is mlaE [H]

Identifier: 126451565

GI number: 126451565

Start: 3644483

End: 3645250

Strand: Reverse

Name: mlaE [H]

Synonym: BURPS1106A_3734

Alternate gene names: 126451565

Gene position: 3645250-3644483 (Counterclockwise)

Preceding gene: 126451478

Following gene: 126454511

Centisome position: 91.4

GC content: 65.49

Gene sequence:

>768_bases
ATGATCAGCGCGATCGGGCGTTTCGTGATCGGCGGACTCGAACGCACGGGCTATGCGACCCGGATGTTCGTGCGCGTCGT
GCTCGAATTCTTCTCGCTGTTGCGGCGGCCGCGGCTCGTCACGAAGCAGATCCATTTTCTCGGCAATTATTCGTTCGTGA
TCATCGCCGTGTCGGGCCTGTTCGTCGGCTTCGTGCTCGGCCTGCAGGGCTACTACACGCTGAACCGCTACGGCTCCGAG
CAGGCGTTGGGCCTGCTCGTCGCGCTGTCGCTCGTGCGCGAGCTCGGCCCCGTCGTGTCGGCGCTGCTGTTCGCCGGCCG
CGCGGGCACGTCGCTCACGGCCGAGATCGGCCTGATGAAGGCGGGCGAGCAACTGACCGCGCTCGAGATGATGGCCGTCG
ATCCGCTGAAGAACGTGATCGCGCCGCGGATGTGGGCGGGCGTCATCGCGATGCCGCTGCTCGCGGCGATCTTCAGCGCG
GTCGGCGTGCTCGGCGGCTATGTCGTCGGCGTGCTGCTGATCGGCGTCGATCCCGGCGCGTTCTGGTCGCAGATGCAAGG
CGGCGTCGAAGTGTGGGCCGACGTCGGCAACGGCGTGATCAAGAGCGTCGTGTTCGGCTTCGCCGTCACGTTTATCGCGC
TGTTTCAGGGTTACGAAGCGAAGCCGACACCCGAGGGCGTGTCGCACGCGACGACCAAGACCGTCGTGTATGCGTCGCTC
GCCGTACTCGGCCTCGATTTTCTGCTGACCGCGTTGATGTTCAGCTAA

Upstream 100 bases:

>100_bases
GCGTGCGCCAGTTCATCGACGGCGCGCCGGACGGCCCGTTCAAATTTCACTACATGAGCCAGCCGCTCGCGGCGGACTTC
GGACTGGGCGGAGGGCGCAG

Downstream 100 bases:

>100_bases
GCCTTTTCTGGGATGACGATGAAAAAGACTGCTCTCGACTTCTGGGTCGGCCTCTTCGTAGTGTTGGGCTTTCTCGCGTT
GCTGTTCCTCGCGCTGAAGG

Product: toluene tolerance ABC transporter, permease protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 255; Mature: 255

Protein sequence:

>255_residues
MISAIGRFVIGGLERTGYATRMFVRVVLEFFSLLRRPRLVTKQIHFLGNYSFVIIAVSGLFVGFVLGLQGYYTLNRYGSE
QALGLLVALSLVRELGPVVSALLFAGRAGTSLTAEIGLMKAGEQLTALEMMAVDPLKNVIAPRMWAGVIAMPLLAAIFSA
VGVLGGYVVGVLLIGVDPGAFWSQMQGGVEVWADVGNGVIKSVVFGFAVTFIALFQGYEAKPTPEGVSHATTKTVVYASL
AVLGLDFLLTALMFS

Sequences:

>Translated_255_residues
MISAIGRFVIGGLERTGYATRMFVRVVLEFFSLLRRPRLVTKQIHFLGNYSFVIIAVSGLFVGFVLGLQGYYTLNRYGSE
QALGLLVALSLVRELGPVVSALLFAGRAGTSLTAEIGLMKAGEQLTALEMMAVDPLKNVIAPRMWAGVIAMPLLAAIFSA
VGVLGGYVVGVLLIGVDPGAFWSQMQGGVEVWADVGNGVIKSVVFGFAVTFIALFQGYEAKPTPEGVSHATTKTVVYASL
AVLGLDFLLTALMFS
>Mature_255_residues
MISAIGRFVIGGLERTGYATRMFVRVVLEFFSLLRRPRLVTKQIHFLGNYSFVIIAVSGLFVGFVLGLQGYYTLNRYGSE
QALGLLVALSLVRELGPVVSALLFAGRAGTSLTAEIGLMKAGEQLTALEMMAVDPLKNVIAPRMWAGVIAMPLLAAIFSA
VGVLGGYVVGVLLIGVDPGAFWSQMQGGVEVWADVGNGVIKSVVFGFAVTFIALFQGYEAKPTPEGVSHATTKTVVYASL
AVLGLDFLLTALMFS

Specific function: Part of the ABC transporter complex mlaFEDB that actively prevents phospholipid accumulation at the cell surface. Probably maintains lipid asymmetry in the outer membrane by retrograde trafficking of phospholipids from the outer membrane to the inner memb

COG id: COG0767

COG function: function code Q; ABC-type transport system involved in resistance to organic solvents, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mlaE permease family [H]

Homologues:

Organism=Escherichia coli, GI1789585, Length=241, Percent_Identity=59.3360995850622, Blast_Score=271, Evalue=3e-74,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003453 [H]

Pfam domain/function: PF02405 DUF140 [H]

EC number: NA

Molecular weight: Translated: 27133; Mature: 27133

Theoretical pI: Translated: 9.55; Mature: 9.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHCC
>Mature Secondary Structure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HHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]