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The map label for this gene is grpE

Identifier: 126451559

GI number: 126451559

Start: 3231438

End: 3231995

Strand: Reverse

Name: grpE

Synonym: BURPS1106A_3313

Alternate gene names: 126451559

Gene position: 3231995-3231438 (Counterclockwise)

Preceding gene: 126452935

Following gene: 126455111

Centisome position: 81.03

GC content: 68.28

Gene sequence:

>558_bases
ATGGAAAACACGCAAGAGAACCCGACTGACCAAACGACTGAAGAAACCGGTCGCGAGGCGCAGGCTGCGGAACCGGCGGC
GCAAGCCGCGGAAAACGCGGCGCCGGCTGCCGAGGCCGCGCTCGCCGAGGCTCAAGCCAAGATCGCCGAGCTGCAGGAGA
GCTTCCTGCGCGCGAAGGCGGAAACCGAGAACGTGCGCCGCCGTGCGCAGGACGACGTGGCGAAGGCGCACAAGTTCGCG
ATCGAGGGTTTCGCCGAGAACCTGTTGCCCGTGCTCGACAGCCTGGAGGCGGCGGTCGGCGATACGTCCGGCGATCTCGC
GAAAGTGCGCGAGGGCGTCGAGCTGACGCTGCGCCAGTTGACGAGCGCGCTCGAGAAGGGCCGCGTCGCGGCGCTCAACC
CGGTCGGCGAGAAGTTCGATCCGCATCTGCACCAGGCGATCTCGATGGTGCCGGCCGACCAGGAACCGAACACGGTCGTC
GCCGTGCTGCAGAAGGGCTACACGATCGCCGACCGCGTGCTGCGCCCGGCGCTCGTGACGGTCGCCCAGCCGAAGTAA

Upstream 100 bases:

>100_bases
CCATTTGCACCGATATTGTGCGGCGCCGGCCGTTCGCCCGAACGAGGCGCGAAGCCGGCTGCCGTTTTGGCTTTAACCTC
TAAATCGACTGTCTAGCGAT

Downstream 100 bases:

>100_bases
GGGCAAGGGGCGGCGATGGCGGGCACGGGCGTCGACGCGGCCGCGTTCGCGGCGTTCGACATGCAGGCGCTCGACGCCGA
GCACTTCGACGCCGGGCTCG

Product: heat shock protein GrpE

Products: NA

Alternate protein names: HSP-70 cofactor

Number of amino acids: Translated: 185; Mature: 185

Protein sequence:

>185_residues
MENTQENPTDQTTEETGREAQAAEPAAQAAENAAPAAEAALAEAQAKIAELQESFLRAKAETENVRRRAQDDVAKAHKFA
IEGFAENLLPVLDSLEAAVGDTSGDLAKVREGVELTLRQLTSALEKGRVAALNPVGEKFDPHLHQAISMVPADQEPNTVV
AVLQKGYTIADRVLRPALVTVAQPK

Sequences:

>Translated_185_residues
MENTQENPTDQTTEETGREAQAAEPAAQAAENAAPAAEAALAEAQAKIAELQESFLRAKAETENVRRRAQDDVAKAHKFA
IEGFAENLLPVLDSLEAAVGDTSGDLAKVREGVELTLRQLTSALEKGRVAALNPVGEKFDPHLHQAISMVPADQEPNTVV
AVLQKGYTIADRVLRPALVTVAQPK
>Mature_185_residues
MENTQENPTDQTTEETGREAQAAEPAAQAAENAAPAAEAALAEAQAKIAELQESFLRAKAETENVRRRAQDDVAKAHKFA
IEGFAENLLPVLDSLEAAVGDTSGDLAKVREGVELTLRQLTSALEKGRVAALNPVGEKFDPHLHQAISMVPADQEPNTVV
AVLQKGYTIADRVLRPALVTVAQPK

Specific function: Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with dnaK and grpE. It is the nucleotide exchange factor for dnaK and may function as a thermosensor. Unfolded

COG id: COG0576

COG function: function code O; Molecular chaperone GrpE (heat shock protein)

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the grpE family

Homologues:

Organism=Homo sapiens, GI24308295, Length=192, Percent_Identity=36.4583333333333, Blast_Score=104, Evalue=5e-23,
Organism=Homo sapiens, GI40255109, Length=191, Percent_Identity=34.0314136125654, Blast_Score=86, Evalue=1e-17,
Organism=Escherichia coli, GI1788967, Length=193, Percent_Identity=41.9689119170984, Blast_Score=125, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI17552458, Length=172, Percent_Identity=33.1395348837209, Blast_Score=83, Evalue=8e-17,
Organism=Saccharomyces cerevisiae, GI6324806, Length=154, Percent_Identity=33.1168831168831, Blast_Score=91, Evalue=7e-20,
Organism=Drosophila melanogaster, GI24653432, Length=177, Percent_Identity=35.0282485875706, Blast_Score=95, Evalue=3e-20,

Paralogues:

None

Copy number: 2359 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): GRPE_BURM7 (A3MNA1)

Other databases:

- EMBL:   CP000548
- RefSeq:   YP_001081737.1
- ProteinModelPortal:   A3MNA1
- SMR:   A3MNA1
- GeneID:   4892348
- GenomeReviews:   CP000548_GR
- KEGG:   bmn:BMA10247_2208
- TIGR:   BMA10247_2208
- HOGENOM:   HBG732630
- OMA:   NLHQAVM
- ProtClustDB:   PRK14139
- BioCyc:   BMAL320389:BMA10247_2208-MONOMER
- GO:   GO:0005739
- HAMAP:   MF_01151
- InterPro:   IPR000740
- InterPro:   IPR013805
- InterPro:   IPR009012
- Gene3D:   G3DSA:3.90.20.20
- Gene3D:   G3DSA:2.30.22.10
- PANTHER:   PTHR21237
- PRINTS:   PR00773

Pfam domain/function: PF01025 GrpE; SSF51064 GrpE_head

EC number: NA

Molecular weight: Translated: 19741; Mature: 19741

Theoretical pI: Translated: 4.49; Mature: 4.49

Prosite motif: PS01071 GRPE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENTQENPTDQTTEETGREAQAAEPAAQAAENAAPAAEAALAEAQAKIAELQESFLRAKA
CCCCCCCCCCCHHHHHCCHHHHCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
ETENVRRRAQDDVAKAHKFAIEGFAENLLPVLDSLEAAVGDTSGDLAKVREGVELTLRQL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
TSALEKGRVAALNPVGEKFDPHLHQAISMVPADQEPNTVVAVLQKGYTIADRVLRPALVT
HHHHHCCCEEEECCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHH
VAQPK
HCCCC
>Mature Secondary Structure
MENTQENPTDQTTEETGREAQAAEPAAQAAENAAPAAEAALAEAQAKIAELQESFLRAKA
CCCCCCCCCCCHHHHHCCHHHHCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
ETENVRRRAQDDVAKAHKFAIEGFAENLLPVLDSLEAAVGDTSGDLAKVREGVELTLRQL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
TSALEKGRVAALNPVGEKFDPHLHQAISMVPADQEPNTVVAVLQKGYTIADRVLRPALVT
HHHHHCCCEEEECCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHH
VAQPK
HCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA