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The map label for this gene is dmlA [H]

Identifier: 284159969

GI number: 284159969

Start: 9863

End: 10948

Strand: Reverse

Name: dmlA [H]

Synonym: BURPS668_A0015

Alternate gene names: 284159969

Gene position: 10948-9863 (Counterclockwise)

Preceding gene: 126445418

Following gene: 126443517

Centisome position: 0.35

GC content: 67.31

Gene sequence:

>1086_bases
ATGAGCGAGAAGGTGTACAGGATCGCCGTCATCCCCGGCGACGGGATCGGCGTGGAAGTGATGCCCGAAGGGCTGCGCGC
GCTCGACGCGGTGAGCCGCCGCTTCGGCTTGCGCTTCGCATACGAGCCGATCGAATGGGCAAGCTGCGACTATTACGCGA
AGCACGGGCAGATGATGCCCGACGACTGGAAGACGCAACTGTCCGGCATGGACGCGCTGCTGTTCGGCGCGGTGGGCTGG
CCCGAGACGGTGCCCGATCACATATCGCTGTGGGGCTCGCTGATCAAATTCCGCCGCGAGTTCGATCAATACGTGAACCT
GCGTCCCGCGCGCCTGTTCGACGGCGTGCCGTGCCCGCTCGCCGGCCGCAAGGCGGGCGACATCGATTTCATGATCGTGC
GCGAGAACACCGAGGGCGAATACTCGGCCGTCGGCGGCACGATGTTCAAGGGCACCGAGCGCGAGTTCGTCGTCCAGCAG
GCGGTGTTCACGCGGCACGGCACCGAGCGCGTGCTGAAGTTCGCGTTCGAGCTCGCGCAGCGCCGCGCAAAGCGGCTCAC
CGTCGCGACGAAGAGCAACGGCATCGCGATCAGCATGCCGTGGTGGGACGCGCGCGCCGCCGAGATGGCCGCACGCTATC
CGGACGTCGCATGGGACAAGCAGCATATCGACATCCTGTGCGCACGCTTCGTGATGCAGCCGGACCGCTTCGACGTCGTC
GTCGCGTCGAACCTGTTCGGCGATATCCTGTCCGATCTCGGCCCCGCCTGCACCGGCACGATCGGCATCGCGCCGTCGGC
GAACCTGAATCCGGAGCGCAACTTTCCGTCGCTGTTCGAGCCGGTGCACGGGTCCGCGCCGGATATCGCCGGCAAGCACA
TTGCCAACCCGATCGCGATGATCTGGTCGGCCGCGATGATGGTCGATTTCCTCGGCGCCGGCACGGGCCCCGCGCGCGAA
GCGCACGATGCGATCGTCGCCGCGATCGAGCACGTGCTGAAGCACGGCCCGCGCACGCGCGACATGGGCGGCCGAGCATC
GACGCGCGAAGTGGGCGACGCAATCGTCGCGCACCTGCTCGGCTGA

Upstream 100 bases:

>100_bases
TCGGCGATCGCGCAAGCTCCGCGCGATCGCTTTCCCGACGCCGCCCGCGCCTGGCGCGACGACGCGCGCGCGGCAGGATA
CGAAGGACGAGGACCTCAGC

Downstream 100 bases:

>100_bases
ACGCCGAACGCACGCGCGCGTCGCGCGTCGCGCGCCGCTTCGACACCACTTTCCGTCGGGATCGCGCCACGGGCTCGACG
GCTATCGCGCAAGCGAGTCT

Product: tartrate dehydrogenase

Products: NA

Alternate protein names: D-malate degradation protein A; D-malate oxidase [H]

Number of amino acids: Translated: 361; Mature: 360

Protein sequence:

>361_residues
MSEKVYRIAVIPGDGIGVEVMPEGLRALDAVSRRFGLRFAYEPIEWASCDYYAKHGQMMPDDWKTQLSGMDALLFGAVGW
PETVPDHISLWGSLIKFRREFDQYVNLRPARLFDGVPCPLAGRKAGDIDFMIVRENTEGEYSAVGGTMFKGTEREFVVQQ
AVFTRHGTERVLKFAFELAQRRAKRLTVATKSNGIAISMPWWDARAAEMAARYPDVAWDKQHIDILCARFVMQPDRFDVV
VASNLFGDILSDLGPACTGTIGIAPSANLNPERNFPSLFEPVHGSAPDIAGKHIANPIAMIWSAAMMVDFLGAGTGPARE
AHDAIVAAIEHVLKHGPRTRDMGGRASTREVGDAIVAHLLG

Sequences:

>Translated_361_residues
MSEKVYRIAVIPGDGIGVEVMPEGLRALDAVSRRFGLRFAYEPIEWASCDYYAKHGQMMPDDWKTQLSGMDALLFGAVGW
PETVPDHISLWGSLIKFRREFDQYVNLRPARLFDGVPCPLAGRKAGDIDFMIVRENTEGEYSAVGGTMFKGTEREFVVQQ
AVFTRHGTERVLKFAFELAQRRAKRLTVATKSNGIAISMPWWDARAAEMAARYPDVAWDKQHIDILCARFVMQPDRFDVV
VASNLFGDILSDLGPACTGTIGIAPSANLNPERNFPSLFEPVHGSAPDIAGKHIANPIAMIWSAAMMVDFLGAGTGPARE
AHDAIVAAIEHVLKHGPRTRDMGGRASTREVGDAIVAHLLG
>Mature_360_residues
SEKVYRIAVIPGDGIGVEVMPEGLRALDAVSRRFGLRFAYEPIEWASCDYYAKHGQMMPDDWKTQLSGMDALLFGAVGWP
ETVPDHISLWGSLIKFRREFDQYVNLRPARLFDGVPCPLAGRKAGDIDFMIVRENTEGEYSAVGGTMFKGTEREFVVQQA
VFTRHGTERVLKFAFELAQRRAKRLTVATKSNGIAISMPWWDARAAEMAARYPDVAWDKQHIDILCARFVMQPDRFDVVV
ASNLFGDILSDLGPACTGTIGIAPSANLNPERNFPSLFEPVHGSAPDIAGKHIANPIAMIWSAAMMVDFLGAGTGPAREA
HDAIVAAIEHVLKHGPRTRDMGGRASTREVGDAIVAHLLG

Specific function: Catalyzes the NAD(+)-dependent oxidative decarboxylation of D-malate into pyruvate. Is essential for aerobic growth on D- malate as the sole carbon source. But is not required for anaerobic D-malate utilization, although DmlA is expressed and active in th

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family [H]

Homologues:

Organism=Homo sapiens, GI5031777, Length=359, Percent_Identity=33.1476323119777, Blast_Score=175, Evalue=6e-44,
Organism=Homo sapiens, GI28178821, Length=365, Percent_Identity=27.3972602739726, Blast_Score=129, Evalue=6e-30,
Organism=Homo sapiens, GI28178816, Length=365, Percent_Identity=27.3972602739726, Blast_Score=126, Evalue=3e-29,
Organism=Homo sapiens, GI4758582, Length=360, Percent_Identity=27.5, Blast_Score=126, Evalue=4e-29,
Organism=Homo sapiens, GI28178838, Length=312, Percent_Identity=28.2051282051282, Blast_Score=115, Evalue=7e-26,
Organism=Homo sapiens, GI28178819, Length=203, Percent_Identity=30.0492610837438, Blast_Score=96, Evalue=4e-20,
Organism=Escherichia coli, GI1788101, Length=358, Percent_Identity=70.6703910614525, Blast_Score=541, Evalue=1e-155,
Organism=Escherichia coli, GI87081683, Length=367, Percent_Identity=40.0544959128065, Blast_Score=221, Evalue=7e-59,
Organism=Escherichia coli, GI1787381, Length=403, Percent_Identity=29.0322580645161, Blast_Score=114, Evalue=9e-27,
Organism=Caenorhabditis elegans, GI71986051, Length=355, Percent_Identity=32.6760563380282, Blast_Score=155, Evalue=2e-38,
Organism=Caenorhabditis elegans, GI17550882, Length=358, Percent_Identity=28.7709497206704, Blast_Score=147, Evalue=7e-36,
Organism=Caenorhabditis elegans, GI25144293, Length=354, Percent_Identity=26.8361581920904, Blast_Score=123, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI17505779, Length=356, Percent_Identity=25.8426966292135, Blast_Score=110, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6322097, Length=373, Percent_Identity=35.9249329758713, Blast_Score=200, Evalue=3e-52,
Organism=Saccharomyces cerevisiae, GI6324709, Length=364, Percent_Identity=32.967032967033, Blast_Score=166, Evalue=5e-42,
Organism=Saccharomyces cerevisiae, GI6319830, Length=368, Percent_Identity=33.4239130434783, Blast_Score=152, Evalue=6e-38,
Organism=Saccharomyces cerevisiae, GI6324291, Length=363, Percent_Identity=28.9256198347107, Blast_Score=129, Evalue=1e-30,
Organism=Drosophila melanogaster, GI24643270, Length=369, Percent_Identity=33.8753387533875, Blast_Score=181, Evalue=9e-46,
Organism=Drosophila melanogaster, GI24643268, Length=369, Percent_Identity=33.8753387533875, Blast_Score=181, Evalue=1e-45,
Organism=Drosophila melanogaster, GI24661184, Length=362, Percent_Identity=31.4917127071823, Blast_Score=163, Evalue=2e-40,
Organism=Drosophila melanogaster, GI161078635, Length=358, Percent_Identity=27.9329608938547, Blast_Score=124, Evalue=7e-29,
Organism=Drosophila melanogaster, GI161078637, Length=358, Percent_Identity=27.9329608938547, Blast_Score=124, Evalue=9e-29,
Organism=Drosophila melanogaster, GI161078633, Length=358, Percent_Identity=27.9329608938547, Blast_Score=124, Evalue=9e-29,
Organism=Drosophila melanogaster, GI24650122, Length=358, Percent_Identity=27.9329608938547, Blast_Score=124, Evalue=9e-29,
Organism=Drosophila melanogaster, GI161078639, Length=354, Percent_Identity=28.2485875706215, Blast_Score=123, Evalue=2e-28,
Organism=Drosophila melanogaster, GI281362242, Length=359, Percent_Identity=27.0194986072423, Blast_Score=112, Evalue=5e-25,
Organism=Drosophila melanogaster, GI24648872, Length=359, Percent_Identity=27.0194986072423, Blast_Score=112, Evalue=5e-25,
Organism=Drosophila melanogaster, GI20130355, Length=361, Percent_Identity=26.0387811634349, Blast_Score=107, Evalue=1e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019818
- InterPro:   IPR001804
- InterPro:   IPR011829 [H]

Pfam domain/function: PF00180 Iso_dh [H]

EC number: =1.1.1.83 [H]

Molecular weight: Translated: 39698; Mature: 39567

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS00470 IDH_IMDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEKVYRIAVIPGDGIGVEVMPEGLRALDAVSRRFGLRFAYEPIEWASCDYYAKHGQMMP
CCCCEEEEEEECCCCCCEEECHHHHHHHHHHHHHHCCEEEECCCCCCCCHHHHHCCCCCC
DDWKTQLSGMDALLFGAVGWPETVPDHISLWGSLIKFRREFDQYVNLRPARLFDGVPCPL
HHHHHHHCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCC
AGRKAGDIDFMIVRENTEGEYSAVGGTMFKGTEREFVVQQAVFTRHGTERVLKFAFELAQ
CCCCCCCEEEEEEEECCCCCHHHCCCHHCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHH
RRAKRLTVATKSNGIAISMPWWDARAAEMAARYPDVAWDKQHIDILCARFVMQPDRFDVV
HHHHHEEEEECCCCEEEEECCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCEEE
VASNLFGDILSDLGPACTGTIGIAPSANLNPERNFPSLFEPVHGSAPDIAGKHIANPIAM
EHHHHHHHHHHHCCCHHCCCEECCCCCCCCCCCCCCHHHHCCCCCCCCCCHHHHCCHHHH
IWSAAMMVDFLGAGTGPAREAHDAIVAAIEHVLKHGPRTRDMGGRASTREVGDAIVAHLL
HHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHC
G
C
>Mature Secondary Structure 
SEKVYRIAVIPGDGIGVEVMPEGLRALDAVSRRFGLRFAYEPIEWASCDYYAKHGQMMP
CCCEEEEEEECCCCCCEEECHHHHHHHHHHHHHHCCEEEECCCCCCCCHHHHHCCCCCC
DDWKTQLSGMDALLFGAVGWPETVPDHISLWGSLIKFRREFDQYVNLRPARLFDGVPCPL
HHHHHHHCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCC
AGRKAGDIDFMIVRENTEGEYSAVGGTMFKGTEREFVVQQAVFTRHGTERVLKFAFELAQ
CCCCCCCEEEEEEEECCCCCHHHCCCHHCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHH
RRAKRLTVATKSNGIAISMPWWDARAAEMAARYPDVAWDKQHIDILCARFVMQPDRFDVV
HHHHHEEEEECCCCEEEEECCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCEEE
VASNLFGDILSDLGPACTGTIGIAPSANLNPERNFPSLFEPVHGSAPDIAGKHIANPIAM
EHHHHHHHHHHHCCCHHCCCEECCCCCCCCCCCCCCHHHHCCCCCCCCCCHHHHCCHHHH
IWSAAMMVDFLGAGTGPAREAHDAIVAAIEHVLKHGPRTRDMGGRASTREVGDAIVAHLL
HHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHC
G
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503 [H]