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The map label for this gene is hsrA [H]

Identifier: 126444986

GI number: 126444986

Start: 962347

End: 963807

Strand: Reverse

Name: hsrA [H]

Synonym: BURPS668_A0992

Alternate gene names: 126444986

Gene position: 963807-962347 (Counterclockwise)

Preceding gene: 126442414

Following gene: 126442936

Centisome position: 30.82

GC content: 68.65

Gene sequence:

>1461_bases
TTGCGCGCGCAAGCATGCCGCAACAATCGATGCAATAATGGCGTCTTTACGCGCGTCGTTGCCATGCCGTCCGCCCTCTA
CGCTTTCATCGCACCGCTGATCGTCGCTTGCGCGTTGTTCATGGAGAGCGTGGATGCCAACATCATCGTCACGGCATTGC
CCGCGATGGCGCGCGACTTCGGGCAGAACCCCGTCACGCTGAACATCGCGATCACGAGCTACGTCGTCGGCCTCGGCGTG
TTCATCCCGATCTGCGGGTGGCTCGCGGACCGCTTCGGCGCGCGCACCGTGTTCCGCACCGCGATCGGCATCTTCGTCGC
GGGCTCGCTGCTGTGCGCGGCATCGAACAATCTCGAGCTCTTCACGTTCGCGCGCTTCGTGCAAGGCGTCGGCGGCGCGA
TGATGGTGCCCGTCGGGCGCATCATCATCTTCCGCGCGGTGCCGCGCACGGAGCTCGTGCGCGCGATGAACTACCTGAGC
GTGCCCGCGCTCTTCGGGCCGGCGGCCGGGCCGCTCCTCGGCGGCTTCATCACGACGTACCTGCACTGGCGGCTGATCTT
CTTCATCAACGTGCCGATCGGCATCCTCGGCATCTATCTCGCGAACAGGCACATCGCGAACACGCACGAGCTCGATCCCG
GCCCGCTCGACGGGTTCGGCTTCGTGCTGTCCGCCGCCGGCGCGGCGCTCCTGCTGATGGGGCTCACGCTGCTCGACGGC
GCGCTCGTCACGCGCGGCGCGGCGCTCGCGATGGGCGCGACGGGCGCGGCGCTCCTCGGCGGCTACGTGCTGTATGCGCG
GCGCGTCGAGCGGCCGGTGCTCGATCTGCGCTTTCTGCGCATTCCGACCTATCACGCAAGCGTCGTCGGCGGCTCGCTGT
TCCGGATCGGCCTCGGCGCAGTGCCGTTTCTGCTGCCGCTCGCGCTGCAGGAAGGGCTCGGCATGAGCGCGTTCCATTCC
GGCGCGATCACGTGCGCGTCGGCCGTCGGCGGCGCGCTCACGCGGATGCTCGCGCCGCGCACGCTCAAGCGCTTCGGCTT
TCGCACGGTGCTGATGTACAACGCGGCGTTCTCCGGGCTCGCGATCGCCGCGTACGGCGTGTTCCATCCCGGCATGTCGA
CGCTCGCGATCTGGCTCATCGTGCTCGTCGGCGGCATCTTTCCGGCGCTGCAGTTCACGAGCCTCAATTCGATGATCTAC
GCGGAAATCGCGGCGCGCGACGCGGGCCGCGCGACGAGCCTCGGCAGCGTCGTCCAGCAGATGTCGCTCGGCCTCGGCGT
GACGGTCGCGGCGCTCGTGCTGCACGTGTCGCATTGGGCGCAGGGGCATCCGGCGATGGTCTGGTCGGACTTCTGGCCGG
CGTTCGTCGTGGTCGGCCTATGCTCGTTCGCATCGATTCCGATCACGCGCCGGCTGTCGCCGAACGCGGGCGACGAGGTG
GCGCGCGGCAAGCGCGGCTGA

Upstream 100 bases:

>100_bases
GCGCAGACGCGCGCCGCCCCAACGACACCGCCGCGGCACCGCGCGCCGCGGCCCGATCGCACGCGGCGCGCGAACGGCAA
CGCGTGACGGCAACACGTCG

Downstream 100 bases:

>100_bases
AGCAGGCCGCACGGCCGAAGCGGTCAGGAAAACGAAGCAAGCGAAGCCGCGTGCGGGGCGCGCTGCGCGCCGCCGCCGGG
CATGGTCAGCCGGAATTGAG

Product: major facilitator transporter

Products: Proton [Cytoplasm]; drug [Periplasm] [C]

Alternate protein names: High-copy suppressor of rspA [H]

Number of amino acids: Translated: 486; Mature: 486

Protein sequence:

>486_residues
MRAQACRNNRCNNGVFTRVVAMPSALYAFIAPLIVACALFMESVDANIIVTALPAMARDFGQNPVTLNIAITSYVVGLGV
FIPICGWLADRFGARTVFRTAIGIFVAGSLLCAASNNLELFTFARFVQGVGGAMMVPVGRIIIFRAVPRTELVRAMNYLS
VPALFGPAAGPLLGGFITTYLHWRLIFFINVPIGILGIYLANRHIANTHELDPGPLDGFGFVLSAAGAALLLMGLTLLDG
ALVTRGAALAMGATGAALLGGYVLYARRVERPVLDLRFLRIPTYHASVVGGSLFRIGLGAVPFLLPLALQEGLGMSAFHS
GAITCASAVGGALTRMLAPRTLKRFGFRTVLMYNAAFSGLAIAAYGVFHPGMSTLAIWLIVLVGGIFPALQFTSLNSMIY
AEIAARDAGRATSLGSVVQQMSLGLGVTVAALVLHVSHWAQGHPAMVWSDFWPAFVVVGLCSFASIPITRRLSPNAGDEV
ARGKRG

Sequences:

>Translated_486_residues
MRAQACRNNRCNNGVFTRVVAMPSALYAFIAPLIVACALFMESVDANIIVTALPAMARDFGQNPVTLNIAITSYVVGLGV
FIPICGWLADRFGARTVFRTAIGIFVAGSLLCAASNNLELFTFARFVQGVGGAMMVPVGRIIIFRAVPRTELVRAMNYLS
VPALFGPAAGPLLGGFITTYLHWRLIFFINVPIGILGIYLANRHIANTHELDPGPLDGFGFVLSAAGAALLLMGLTLLDG
ALVTRGAALAMGATGAALLGGYVLYARRVERPVLDLRFLRIPTYHASVVGGSLFRIGLGAVPFLLPLALQEGLGMSAFHS
GAITCASAVGGALTRMLAPRTLKRFGFRTVLMYNAAFSGLAIAAYGVFHPGMSTLAIWLIVLVGGIFPALQFTSLNSMIY
AEIAARDAGRATSLGSVVQQMSLGLGVTVAALVLHVSHWAQGHPAMVWSDFWPAFVVVGLCSFASIPITRRLSPNAGDEV
ARGKRG
>Mature_486_residues
MRAQACRNNRCNNGVFTRVVAMPSALYAFIAPLIVACALFMESVDANIIVTALPAMARDFGQNPVTLNIAITSYVVGLGV
FIPICGWLADRFGARTVFRTAIGIFVAGSLLCAASNNLELFTFARFVQGVGGAMMVPVGRIIIFRAVPRTELVRAMNYLS
VPALFGPAAGPLLGGFITTYLHWRLIFFINVPIGILGIYLANRHIANTHELDPGPLDGFGFVLSAAGAALLLMGLTLLDG
ALVTRGAALAMGATGAALLGGYVLYARRVERPVLDLRFLRIPTYHASVVGGSLFRIGLGAVPFLLPLALQEGLGMSAFHS
GAITCASAVGGALTRMLAPRTLKRFGFRTVLMYNAAFSGLAIAAYGVFHPGMSTLAIWLIVLVGGIFPALQFTSLNSMIY
AEIAARDAGRATSLGSVVQQMSLGLGVTVAALVLHVSHWAQGHPAMVWSDFWPAFVVVGLCSFASIPITRRLSPNAGDEV
ARGKRG

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1790195, Length=457, Percent_Identity=36.7614879649891, Blast_Score=296, Evalue=2e-81,
Organism=Escherichia coli, GI1788392, Length=406, Percent_Identity=36.6995073891626, Blast_Score=221, Evalue=8e-59,
Organism=Escherichia coli, GI1789042, Length=416, Percent_Identity=25.9615384615385, Blast_Score=112, Evalue=4e-26,
Organism=Escherichia coli, GI1788710, Length=409, Percent_Identity=24.6943765281174, Blast_Score=102, Evalue=6e-23,
Organism=Escherichia coli, GI87081983, Length=295, Percent_Identity=28.135593220339, Blast_Score=98, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6322958, Length=264, Percent_Identity=29.5454545454545, Blast_Score=76, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6325455, Length=231, Percent_Identity=26.4069264069264, Blast_Score=66, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR001411 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 51386; Mature: 51386

Theoretical pI: Translated: 10.39; Mature: 10.39

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRAQACRNNRCNNGVFTRVVAMPSALYAFIAPLIVACALFMESVDANIIVTALPAMARDF
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHH
GQNPVTLNIAITSYVVGLGVFIPICGWLADRFGARTVFRTAIGIFVAGSLLCAASNNLEL
CCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCHH
FTFARFVQGVGGAMMVPVGRIIIFRAVPRTELVRAMNYLSVPALFGPAAGPLLGGFITTY
HHHHHHHHCCCCEEHHHHHHEEEEECCCHHHHHHHHHHHHCHHHCCCCHHHHHHHHHHHH
LHWRLIFFINVPIGILGIYLANRHIANTHELDPGPLDGFGFVLSAAGAALLLMGLTLLDG
HHHHHHHHHHCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
ALVTRGAALAMGATGAALLGGYVLYARRVERPVLDLRFLRIPTYHASVVGGSLFRIGLGA
HHHHCCHHHHCCCCHHHHHHHHHHHHHHHCCCHHHEEEEECCCHHHHHHCCHHHHHHHHH
VPFLLPLALQEGLGMSAFHSGAITCASAVGGALTRMLAPRTLKRFGFRTVLMYNAAFSGL
HHHHHHHHHHHCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
AIAAYGVFHPGMSTLAIWLIVLVGGIFPALQFTSLNSMIYAEIAARDAGRATSLGSVVQQ
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
MSLGLGVTVAALVLHVSHWAQGHPAMVWSDFWPAFVVVGLCSFASIPITRRLSPNAGDEV
HHCCCHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHCCCCEECCCCCCCCHH
ARGKRG
HCCCCC
>Mature Secondary Structure
MRAQACRNNRCNNGVFTRVVAMPSALYAFIAPLIVACALFMESVDANIIVTALPAMARDF
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHH
GQNPVTLNIAITSYVVGLGVFIPICGWLADRFGARTVFRTAIGIFVAGSLLCAASNNLEL
CCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCHH
FTFARFVQGVGGAMMVPVGRIIIFRAVPRTELVRAMNYLSVPALFGPAAGPLLGGFITTY
HHHHHHHHCCCCEEHHHHHHEEEEECCCHHHHHHHHHHHHCHHHCCCCHHHHHHHHHHHH
LHWRLIFFINVPIGILGIYLANRHIANTHELDPGPLDGFGFVLSAAGAALLLMGLTLLDG
HHHHHHHHHHCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
ALVTRGAALAMGATGAALLGGYVLYARRVERPVLDLRFLRIPTYHASVVGGSLFRIGLGA
HHHHCCHHHHCCCCHHHHHHHHHHHHHHHCCCHHHEEEEECCCHHHHHHCCHHHHHHHHH
VPFLLPLALQEGLGMSAFHSGAITCASAVGGALTRMLAPRTLKRFGFRTVLMYNAAFSGL
HHHHHHHHHHHCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
AIAAYGVFHPGMSTLAIWLIVLVGGIFPALQFTSLNSMIYAEIAARDAGRATSLGSVVQQ
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
MSLGLGVTVAALVLHVSHWAQGHPAMVWSDFWPAFVVVGLCSFASIPITRRLSPNAGDEV
HHCCCHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHCCCCEECCCCCCCCHH
ARGKRG
HCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; drug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + drug [Cytoplasm] = Proton [Cytoplasm] + drug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7686882; 9278503 [H]