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The map label for this gene is oprM [H]

Identifier: 126444944

GI number: 126444944

Start: 1039395

End: 1041254

Strand: Reverse

Name: oprM [H]

Synonym: BURPS668_A1081

Alternate gene names: 126444944

Gene position: 1041254-1039395 (Counterclockwise)

Preceding gene: 126445531

Following gene: 126443794

Centisome position: 33.29

GC content: 70.86

Gene sequence:

>1860_bases
ATGCATAGCCCACCGTTTCTTTCATCGCCCAAGCGCGCGCTGCCGATCGCGCTCGCGCTCGGCCTCGCCGGTTGCTCGAT
GGAGCCGGCCTACCAGCGGCCCGATGCGCCGATGCCGTCCGCGTATCCGAACGGCCCCGCCTACGCGACGCCGGGCGCGC
CGCGCGCGAACCCGGGCGAGCCGGCGGCGGCCGAGCTCGGCTGGCGGAATTTCCTCGCCGACGCGCAGTTGCAGCAGCTC
GTCGCGCTCGCGCTCGCGAACAACCGCGACCTGCGCGTCGCGACGCTCGACATCGACGAAGCGCGCGCGCTGTACCGCAT
CCAGCGCGCCGCGCAGTTTCCCGCAATCGATGCGAGCGTCGGACTCACGAGCCAGCGCATGAGCCCCGCGCTGCGCGCGC
CGGGGCAGTCCGCGGCGATCAACAGCTATGACGCGAGCGTCGGCCTCACCCATTTCGAGATCGACCTGTTCGGCCGCATG
CGCAGCCTGAGCCACGCCGCGCAGGAGCAATACCTCGCGACCGAGGAGGCGCGGCGCAGCGTCCACATCAGCCTCGTCGC
CGAAGTCGCGAACACCTACCTGACGCTGCTCGCCGATCGCGCGCTGCTCGCGCTCGCGCAGGACACGCTCAGGAGCCAGC
AGGACGCCGCCGACATGATCCACCGCGGCAAGCAGGCGGGCGCGATGGCGCAGCTCGACGAGCATCGCGCGGACACGCAG
GTGCAGACCGCACGCGTCGCCGCCGAGCAATACACGCGGCAGATCGCGCAGGACGAGAACGCGCTTGCCGTGCTGATCGG
CGGGCCGCTGCCGGCCGGCGTGTCGCGCGCGGCGCCGCTCGACGATCGCGCGCTGCTCGCGGAGTTCCCGGCGGGCCTGC
CGTCGACGCTGCTCGAGCGCCGCCCCGACATCATGGCCGCCGAGCATCGGCTCATCGCCGCGAACGCGAATATCGGCGCG
GCGCGCGCGGCGTTCTTCCCGAGGATCACGTTGACGGGCGCGCTCGGCGTGGCGAGCGCGAGCCTCGCGGGGCTGTTCTC
CGGCGGCGTCGCGTGGCTGTTCGTGCCGCAGCTCACGCTGCCGATCTTCAACGCGGGCAGCAATCAGGCGAATCTCGATC
TCGCGACGGTGCGCCGCGACATCAACGTGGCCGGCTACGAGCACACGATCCAGGACGCGTTCCGCGAGGTCGCCGACAAC
CTGGCCGCACGCGCGACCTACGAGCGGGAAGTGAAAGCGCAGGAGGCGATGATCCGCGACCTGGCCGAGACGAAGCGGCT
GGCCGACATGCGTTTTCGCAACGGCGTCGACGACTACTTCGGCGTGTTCGATGCGCAACGCCAGCTGTTCGCCGCGCAGC
AACTGCTCGTCACCTACAAGCTTGCCGGGCTGACGAGCCGCGTGACGCTATACAAGGCGCTCGGCGGCGGCTGGGTCGAG
TCGGCGGGCGCGGTGGCCGCGCAACCGCGGACGGGGCCGGCCGCGCCGATGGCTCGCCCGGCAGCCACTCCCCCCCAGAC
GACGCGACCCGCGCCGGTTGCTCGATCGCAGACGGTTCCACCGCGAACGGTTCAGCCCCCGATCGCCCAACCGGCAACCG
CGCAACCGCAAACGGCGCAAGCCGCGCCGGTTGCTCAGTCGCAGACGATTCCACTGCGAACGGTCCAGCCTCCTATCGCC
CAATCGTCAACCTCTCAACCGCAAACGGCGCAAGCCGCGCCGATCACTCAAGCGCGGACGACACCACTCCGAACGGCTCA
GCCGCCTGCCGCTCGACAACAGGCGCCGCAATCCGCTCCGGCGGTTCAACCGGAGGCAGCTCCACCGCAAACGGCCCAAC
CGCCGATTTTTCAGCCGTAA

Upstream 100 bases:

>100_bases
GCTCGCGATCTCGCGCCTGATGCAGCGCGCGCTGTCCGGCTGAACGCCGCGAGCGCCGCTTGCCCACCGTCTCGATTCTC
GATGCGAGGAGCCGCCGACC

Downstream 100 bases:

>100_bases
GCAAGGCGACAACATCGCGACGGGTATCGACGCAACGCACGCCCCCGTGCAAGCGACGCGATAAACCGCGCAAAAGACCG
CGCCAGCCGCGCTGAGCGCG

Product: RND efflux system outer membrane lipoprotein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 619; Mature: 619

Protein sequence:

>619_residues
MHSPPFLSSPKRALPIALALGLAGCSMEPAYQRPDAPMPSAYPNGPAYATPGAPRANPGEPAAAELGWRNFLADAQLQQL
VALALANNRDLRVATLDIDEARALYRIQRAAQFPAIDASVGLTSQRMSPALRAPGQSAAINSYDASVGLTHFEIDLFGRM
RSLSHAAQEQYLATEEARRSVHISLVAEVANTYLTLLADRALLALAQDTLRSQQDAADMIHRGKQAGAMAQLDEHRADTQ
VQTARVAAEQYTRQIAQDENALAVLIGGPLPAGVSRAAPLDDRALLAEFPAGLPSTLLERRPDIMAAEHRLIAANANIGA
ARAAFFPRITLTGALGVASASLAGLFSGGVAWLFVPQLTLPIFNAGSNQANLDLATVRRDINVAGYEHTIQDAFREVADN
LAARATYEREVKAQEAMIRDLAETKRLADMRFRNGVDDYFGVFDAQRQLFAAQQLLVTYKLAGLTSRVTLYKALGGGWVE
SAGAVAAQPRTGPAAPMARPAATPPQTTRPAPVARSQTVPPRTVQPPIAQPATAQPQTAQAAPVAQSQTIPLRTVQPPIA
QSSTSQPQTAQAAPITQARTTPLRTAQPPAARQQAPQSAPAVQPEAAPPQTAQPPIFQP

Sequences:

>Translated_619_residues
MHSPPFLSSPKRALPIALALGLAGCSMEPAYQRPDAPMPSAYPNGPAYATPGAPRANPGEPAAAELGWRNFLADAQLQQL
VALALANNRDLRVATLDIDEARALYRIQRAAQFPAIDASVGLTSQRMSPALRAPGQSAAINSYDASVGLTHFEIDLFGRM
RSLSHAAQEQYLATEEARRSVHISLVAEVANTYLTLLADRALLALAQDTLRSQQDAADMIHRGKQAGAMAQLDEHRADTQ
VQTARVAAEQYTRQIAQDENALAVLIGGPLPAGVSRAAPLDDRALLAEFPAGLPSTLLERRPDIMAAEHRLIAANANIGA
ARAAFFPRITLTGALGVASASLAGLFSGGVAWLFVPQLTLPIFNAGSNQANLDLATVRRDINVAGYEHTIQDAFREVADN
LAARATYEREVKAQEAMIRDLAETKRLADMRFRNGVDDYFGVFDAQRQLFAAQQLLVTYKLAGLTSRVTLYKALGGGWVE
SAGAVAAQPRTGPAAPMARPAATPPQTTRPAPVARSQTVPPRTVQPPIAQPATAQPQTAQAAPVAQSQTIPLRTVQPPIA
QSSTSQPQTAQAAPITQARTTPLRTAQPPAARQQAPQSAPAVQPEAAPPQTAQPPIFQP
>Mature_619_residues
MHSPPFLSSPKRALPIALALGLAGCSMEPAYQRPDAPMPSAYPNGPAYATPGAPRANPGEPAAAELGWRNFLADAQLQQL
VALALANNRDLRVATLDIDEARALYRIQRAAQFPAIDASVGLTSQRMSPALRAPGQSAAINSYDASVGLTHFEIDLFGRM
RSLSHAAQEQYLATEEARRSVHISLVAEVANTYLTLLADRALLALAQDTLRSQQDAADMIHRGKQAGAMAQLDEHRADTQ
VQTARVAAEQYTRQIAQDENALAVLIGGPLPAGVSRAAPLDDRALLAEFPAGLPSTLLERRPDIMAAEHRLIAANANIGA
ARAAFFPRITLTGALGVASASLAGLFSGGVAWLFVPQLTLPIFNAGSNQANLDLATVRRDINVAGYEHTIQDAFREVADN
LAARATYEREVKAQEAMIRDLAETKRLADMRFRNGVDDYFGVFDAQRQLFAAQQLLVTYKLAGLTSRVTLYKALGGGWVE
SAGAVAAQPRTGPAAPMARPAATPPQTTRPAPVARSQTVPPRTVQPPIAQPATAQPQTAQAAPVAQSQTIPLRTVQPPIA
QSSTSQPQTAQAAPITQARTTPLRTAQPPAARQQAPQSAPAVQPEAAPPQTAQPPIFQP

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: COG1538

COG function: function code MU; Outer membrane protein

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor. Note=Attached to the outer membrane by a lipid anchor and via the transmembrane beta-barrel. The membrane anchor is not necessary for antibiotic efflux. In one report (PubMed:10889211) the Cys-18- Gly mutant is reported

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI1786785, Length=478, Percent_Identity=41.2133891213389, Blast_Score=329, Evalue=4e-91,
Organism=Escherichia coli, GI2367348, Length=471, Percent_Identity=26.963906581741, Blast_Score=89, Evalue=7e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010131 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 65701; Mature: 65701

Theoretical pI: Translated: 9.37; Mature: 9.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHSPPFLSSPKRALPIALALGLAGCSMEPAYQRPDAPMPSAYPNGPAYATPGAPRANPGE
CCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
PAAAELGWRNFLADAQLQQLVALALANNRDLRVATLDIDEARALYRIQRAAQFPAIDASV
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECHHHHHHHHHHHHHHHCCCCCCCC
GLTSQRMSPALRAPGQSAAINSYDASVGLTHFEIDLFGRMRSLSHAAQEQYLATEEARRS
CCCHHHCCHHHCCCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHH
VHISLVAEVANTYLTLLADRALLALAQDTLRSQQDAADMIHRGKQAGAMAQLDEHRADTQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCHHHHHHHHHCCHH
VQTARVAAEQYTRQIAQDENALAVLIGGPLPAGVSRAAPLDDRALLAEFPAGLPSTLLER
HHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCHHHHHHCCCCCHHHHHHC
RPDIMAAEHRLIAANANIGAARAAFFPRITLTGALGVASASLAGLFSGGVAWLFVPQLTL
CCCHHHHCCEEEEECCCCCHHHHHHCCCEEEEHHHHHHHHHHHHHHHCCEEEEEECHHHC
PIFNAGSNQANLDLATVRRDINVAGYEHTIQDAFREVADNLAARATYEREVKAQEAMIRD
EEECCCCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LAETKRLADMRFRNGVDDYFGVFDAQRQLFAAQQLLVTYKLAGLTSRVTLYKALGGGWVE
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
SAGAVAAQPRTGPAAPMARPAATPPQTTRPAPVARSQTVPPRTVQPPIAQPATAQPQTAQ
CCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
AAPVAQSQTIPLRTVQPPIAQSSTSQPQTAQAAPITQARTTPLRTAQPPAARQQAPQSAP
CCCCCCCCCCCCEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCC
AVQPEAAPPQTAQPPIFQP
CCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MHSPPFLSSPKRALPIALALGLAGCSMEPAYQRPDAPMPSAYPNGPAYATPGAPRANPGE
CCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
PAAAELGWRNFLADAQLQQLVALALANNRDLRVATLDIDEARALYRIQRAAQFPAIDASV
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECHHHHHHHHHHHHHHHCCCCCCCC
GLTSQRMSPALRAPGQSAAINSYDASVGLTHFEIDLFGRMRSLSHAAQEQYLATEEARRS
CCCHHHCCHHHCCCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHH
VHISLVAEVANTYLTLLADRALLALAQDTLRSQQDAADMIHRGKQAGAMAQLDEHRADTQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCHHHHHHHHHCCHH
VQTARVAAEQYTRQIAQDENALAVLIGGPLPAGVSRAAPLDDRALLAEFPAGLPSTLLER
HHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCHHHHHHCCCCCHHHHHHC
RPDIMAAEHRLIAANANIGAARAAFFPRITLTGALGVASASLAGLFSGGVAWLFVPQLTL
CCCHHHHCCEEEEECCCCCHHHHHHCCCEEEEHHHHHHHHHHHHHHHCCEEEEEECHHHC
PIFNAGSNQANLDLATVRRDINVAGYEHTIQDAFREVADNLAARATYEREVKAQEAMIRD
EEECCCCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LAETKRLADMRFRNGVDDYFGVFDAQRQLFAAQQLLVTYKLAGLTSRVTLYKALGGGWVE
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
SAGAVAAQPRTGPAAPMARPAATPPQTTRPAPVARSQTVPPRTVQPPIAQPATAQPQTAQ
CCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
AAPVAQSQTIPLRTVQPPIAQSSTSQPQTAQAAPITQARTTPLRTAQPPAARQQAPQSAP
CCCCCCCCCCCCEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCC
AVQPEAAPPQTAQPPIFQP
CCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8226684; 10889211; 10984043; 7968531; 8540696; 9401051; 9603892; 10952562 [H]