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The map label for this gene is gcvA [H]

Identifier: 126444719

GI number: 126444719

Start: 406850

End: 407746

Strand: Reverse

Name: gcvA [H]

Synonym: BURPS668_A0444

Alternate gene names: 126444719

Gene position: 407746-406850 (Counterclockwise)

Preceding gene: 126445469

Following gene: 126443219

Centisome position: 13.04

GC content: 69.79

Gene sequence:

>897_bases
ATGAAGCATCTGTATCCGAACGTCGCCGAACTGCACGCGTTCGCCAGTTCTGCGAAGCATCTGAACTTTTCGTATGCGGC
GCGCGAGCTCGGCCTCACGCCGAGCGCGGTCAGCCGGCAGATCGCGAGCCTCGAGGCGCTGCTCGGCGTGAAGCTGTTCG
TGCGCGAAGGCCGCAATCTCGCGCTCACGCGCGCGGGGCAGGTCTATCAGGCGCGCGTCGCGGGGCCCCTGCGCGAGATC
GGCAATGCGTCGCTCGAATTGCTGAGCGCGCGCGAGGACAGCAATCTGCTGACCATTGCGAGCGTGCCGACCTTCACGAC
GAAATGGCTCGTGCCGCGCCTGCCGCGCTTTCTCGAAACCGCGCCTGACATCACGCTGAGCTTCCGGCGCCACCTCGCGC
CGGGCGACCTGTTCCCGCTCGGGCTCGATGCGGCGATCCGCTACGGCGACGGCCGCTGGGAAGGCGTCCAGTGCGACTAT
CTCGACGGCCGCACGTTCGTGCCCGTATGCGCGCCCGGATTCGCCGAGCGTTACGCCCTGCGCGAACCCGCGGACATCGC
CGCCGCGCCGCGCCTCGTGCACGAGCAGGCCGAATGCGCGTGGCTCGCGTGGGCGGACCGGCACCGCGCCACGCAGATGA
ACGCGCTCGCCGGCCCACGCTTCGAGCAGTACTCGGTGCTGATCCAGGCGGCGCAGGCAGGGCTCGGGATCGCGCTCATT
CCCGCGTTCCTGATTCGCGCGCCGCTCGCGGCCGGCACGCTCGTGCAGCCGCTCGACGCGCCCGTCGACGTCGACGAGCA
GAGCCATTACCTGTGCTACGCGCCGGAGCGGCTGCAGGCGAGTGCATCGCTGCGGCTGCTGCGCGAATGGATGCTCGGCG
AGTGCGCATGCGCATGA

Upstream 100 bases:

>100_bases
ATCGCGACGACGGCCGCCAGTGTGGGCGCGCGACATGGCGTCGTCATGAAAAAAACGTACAAACTCCGCATCGCTCTTTG
CATCGACGGAATACTTCGCG

Downstream 100 bases:

>100_bases
CGCGCATCGCGCCGCCTTGTCCGCGCCGCCGCACCTGCGCTCTCGTGACGCGGCGAAACCGCGCGCCGCTCGGCTTTTGC
GCGCGGCGGACCTAGTATGA

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 298; Mature: 298

Protein sequence:

>298_residues
MKHLYPNVAELHAFASSAKHLNFSYAARELGLTPSAVSRQIASLEALLGVKLFVREGRNLALTRAGQVYQARVAGPLREI
GNASLELLSAREDSNLLTIASVPTFTTKWLVPRLPRFLETAPDITLSFRRHLAPGDLFPLGLDAAIRYGDGRWEGVQCDY
LDGRTFVPVCAPGFAERYALREPADIAAAPRLVHEQAECAWLAWADRHRATQMNALAGPRFEQYSVLIQAAQAGLGIALI
PAFLIRAPLAAGTLVQPLDAPVDVDEQSHYLCYAPERLQASASLRLLREWMLGECACA

Sequences:

>Translated_298_residues
MKHLYPNVAELHAFASSAKHLNFSYAARELGLTPSAVSRQIASLEALLGVKLFVREGRNLALTRAGQVYQARVAGPLREI
GNASLELLSAREDSNLLTIASVPTFTTKWLVPRLPRFLETAPDITLSFRRHLAPGDLFPLGLDAAIRYGDGRWEGVQCDY
LDGRTFVPVCAPGFAERYALREPADIAAAPRLVHEQAECAWLAWADRHRATQMNALAGPRFEQYSVLIQAAQAGLGIALI
PAFLIRAPLAAGTLVQPLDAPVDVDEQSHYLCYAPERLQASASLRLLREWMLGECACA
>Mature_298_residues
MKHLYPNVAELHAFASSAKHLNFSYAARELGLTPSAVSRQIASLEALLGVKLFVREGRNLALTRAGQVYQARVAGPLREI
GNASLELLSAREDSNLLTIASVPTFTTKWLVPRLPRFLETAPDITLSFRRHLAPGDLFPLGLDAAIRYGDGRWEGVQCDY
LDGRTFVPVCAPGFAERYALREPADIAAAPRLVHEQAECAWLAWADRHRATQMNALAGPRFEQYSVLIQAAQAGLGIALI
PAFLIRAPLAAGTLVQPLDAPVDVDEQSHYLCYAPERLQASASLRLLREWMLGECACA

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=298, Percent_Identity=32.5503355704698, Blast_Score=144, Evalue=7e-36,
Organism=Escherichia coli, GI1788706, Length=277, Percent_Identity=30.3249097472924, Blast_Score=123, Evalue=1e-29,
Organism=Escherichia coli, GI1786448, Length=285, Percent_Identity=31.2280701754386, Blast_Score=123, Evalue=2e-29,
Organism=Escherichia coli, GI145693193, Length=300, Percent_Identity=30, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1786401, Length=249, Percent_Identity=28.9156626506024, Blast_Score=81, Evalue=9e-17,
Organism=Escherichia coli, GI157672245, Length=195, Percent_Identity=31.2820512820513, Blast_Score=69, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32640; Mature: 32640

Theoretical pI: Translated: 7.15; Mature: 7.15

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKHLYPNVAELHAFASSAKHLNFSYAARELGLTPSAVSRQIASLEALLGVKLFVREGRNL
CCCCCCCHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCE
ALTRAGQVYQARVAGPLREIGNASLELLSAREDSNLLTIASVPTFTTKWLVPRLPRFLET
EEEECCCHHHHHHHHHHHHHCCHHEEHHCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHC
APDITLSFRRHLAPGDLFPLGLDAAIRYGDGRWEGVQCDYLDGRTFVPVCAPGFAERYAL
CCCCCHHHHHHCCCCCCCCCCHHHEEEECCCCCCCEEEEEECCCEEEEECCCCHHHHHHC
REPADIAAAPRLVHEQAECAWLAWADRHRATQMNALAGPRFEQYSVLIQAAQAGLGIALI
CCCHHHHHHHHHHHHHHHEEEEEHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHHH
PAFLIRAPLAAGTLVQPLDAPVDVDEQSHYLCYAPERLQASASLRLLREWMLGECACA
HHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKHLYPNVAELHAFASSAKHLNFSYAARELGLTPSAVSRQIASLEALLGVKLFVREGRNL
CCCCCCCHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCE
ALTRAGQVYQARVAGPLREIGNASLELLSAREDSNLLTIASVPTFTTKWLVPRLPRFLET
EEEECCCHHHHHHHHHHHHHCCHHEEHHCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHC
APDITLSFRRHLAPGDLFPLGLDAAIRYGDGRWEGVQCDYLDGRTFVPVCAPGFAERYAL
CCCCCHHHHHHCCCCCCCCCCHHHEEEECCCCCCCEEEEEECCCEEEEECCCCHHHHHHC
REPADIAAAPRLVHEQAECAWLAWADRHRATQMNALAGPRFEQYSVLIQAAQAGLGIALI
CCCHHHHHHHHHHHHHHHEEEEEHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHHH
PAFLIRAPLAAGTLVQPLDAPVDVDEQSHYLCYAPERLQASASLRLLREWMLGECACA
HHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]