Click here to switch to the map view.

The map label for this gene is ilvE [H]

Identifier: 126444335

GI number: 126444335

Start: 2496556

End: 2497518

Strand: Reverse

Name: ilvE [H]

Synonym: BURPS668_A2593

Alternate gene names: 126444335

Gene position: 2497518-2496556 (Counterclockwise)

Preceding gene: 126444613

Following gene: 126442756

Centisome position: 79.86

GC content: 65.42

Gene sequence:

>963_bases
GTGAGGAGCGCAACAACGATGAAATCGAGCTACGTGTGGAAGAACGGTGAACTGGTGCCGTGGGAGCATGCGCAGGTGCA
TGTGATGAGCCATGCGCTGCATTACGGGACGAGTGTGTTCGAAGGCGTGCGCGCGTACGAGATCGGCGAGAAGGCGGCGA
TCCTCTGCGGCCGTGAGCACTTCGAGCGCCTGCTGTTCAGCTGCAAGGTCGCGCGGATTGCGTCGCCGATGAGCGTCGGG
CAATGGATGGAGGCGACGGTCGAGACGCTGCGCGCGAACGGTCAGCGCAGCGCGTACATCCGGCCGCTCGTCTATCGCGG
CGCGGGCGAATCGCTCGGGCTCGACGCGCGACAGTGCCCGGCCGAGGCACTGCTCATCACCACGCCGTGGGGCGCGTATC
TCGGCGACGAGGCGTTGCAGCAAGGCGTCGACGTGCAGGTGAGCAGCTGGCGGCGCAACGGCGCGGGAGCGGCAAGCACG
CTGGCGAAGATCGGCGGCCAGTACGTCAACGGTCAGGCGATCGTGATGGAGGCGCACGAGAACGGGATGAGCGAAGGCAT
CGCGCTCGATACGAACGGTTTCGTCAGCGAAGGCAGCGGCGAGAACGTGTTCCTTGTCTACAAGAACGAAATCTTCACGC
CGTCGATCGGCAGCAGCATCCTGAGCGGCATCACGCGCCACTGCGTGATCCGGATCGCGCGCGATCTCGGCTATACCGTG
ACCGAAACGAGCATTCCGCGCGAGATGCTCTACCTCGCCGACGAAATCTTCTTCACGGGCACCGCCGTCGAGATTTGTCC
CGTTCGCTCGGTCGACCGGATGCCGGTCGGCAGCGGCAAGCGCGGCGCGGTGACGAAGGCGATCCAGGATCAGTACTTCG
GCATCGTGCGCGGCACGCACGCGGACAAATGGAACTGGCTCACGCCCGTGGCGGTGCCGTCGCGCGACGGAGCACGGGCA
TGA

Upstream 100 bases:

>100_bases
TCACGCGCGCGATCGCGAAGAACCGGCTCGAGCGCAAGGTACGCGGCGCGATCGCAGAGGGGCGCAGCGTCGTCGAGGCA
TTCAATCAATACAACGTCAT

Downstream 100 bases:

>100_bases
GCGCGCGCGAAGCCGCAGCCGAATCGCCTTGGCTGATCGTCCATGAGGCGCGCAACGCGCGCGTGCGGCTCTTCTGCTTT
CACTACGCGGGCGCGACCGC

Product: branched-chain amino acid aminotransferase

Products: NA

Alternate protein names: BCAT; Transaminase B [H]

Number of amino acids: Translated: 320; Mature: 320

Protein sequence:

>320_residues
MRSATTMKSSYVWKNGELVPWEHAQVHVMSHALHYGTSVFEGVRAYEIGEKAAILCGREHFERLLFSCKVARIASPMSVG
QWMEATVETLRANGQRSAYIRPLVYRGAGESLGLDARQCPAEALLITTPWGAYLGDEALQQGVDVQVSSWRRNGAGAAST
LAKIGGQYVNGQAIVMEAHENGMSEGIALDTNGFVSEGSGENVFLVYKNEIFTPSIGSSILSGITRHCVIRIARDLGYTV
TETSIPREMLYLADEIFFTGTAVEICPVRSVDRMPVGSGKRGAVTKAIQDQYFGIVRGTHADKWNWLTPVAVPSRDGARA

Sequences:

>Translated_320_residues
MRSATTMKSSYVWKNGELVPWEHAQVHVMSHALHYGTSVFEGVRAYEIGEKAAILCGREHFERLLFSCKVARIASPMSVG
QWMEATVETLRANGQRSAYIRPLVYRGAGESLGLDARQCPAEALLITTPWGAYLGDEALQQGVDVQVSSWRRNGAGAAST
LAKIGGQYVNGQAIVMEAHENGMSEGIALDTNGFVSEGSGENVFLVYKNEIFTPSIGSSILSGITRHCVIRIARDLGYTV
TETSIPREMLYLADEIFFTGTAVEICPVRSVDRMPVGSGKRGAVTKAIQDQYFGIVRGTHADKWNWLTPVAVPSRDGARA
>Mature_320_residues
MRSATTMKSSYVWKNGELVPWEHAQVHVMSHALHYGTSVFEGVRAYEIGEKAAILCGREHFERLLFSCKVARIASPMSVG
QWMEATVETLRANGQRSAYIRPLVYRGAGESLGLDARQCPAEALLITTPWGAYLGDEALQQGVDVQVSSWRRNGAGAAST
LAKIGGQYVNGQAIVMEAHENGMSEGIALDTNGFVSEGSGENVFLVYKNEIFTPSIGSSILSGITRHCVIRIARDLGYTV
TETSIPREMLYLADEIFFTGTAVEICPVRSVDRMPVGSGKRGAVTKAIQDQYFGIVRGTHADKWNWLTPVAVPSRDGARA

Specific function: Acts on leucine, isoleucine and valine [H]

COG id: COG0115

COG function: function code EH; Branched-chain amino acid aminotransferase/4-amino-4-deoxychorismate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Escherichia coli, GI48994963, Length=310, Percent_Identity=49.3548387096774, Blast_Score=298, Evalue=2e-82,
Organism=Saccharomyces cerevisiae, GI6322002, Length=337, Percent_Identity=25.5192878338279, Blast_Score=69, Evalue=1e-12,

Paralogues:

None

Copy number: 2342 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 11,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001544
- InterPro:   IPR018300
- InterPro:   IPR005785 [H]

Pfam domain/function: PF01063 Aminotran_4 [H]

EC number: =2.6.1.42 [H]

Molecular weight: Translated: 34862; Mature: 34862

Theoretical pI: Translated: 7.22; Mature: 7.22

Prosite motif: PS00770 AA_TRANSFER_CLASS_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSATTMKSSYVWKNGELVPWEHAQVHVMSHALHYGTSVFEGVRAYEIGEKAAILCGREH
CCCCCCCHHHEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCHHHHHCCCHHEEEECHHH
FERLLFSCKVARIASPMSVGQWMEATVETLRANGQRSAYIRPLVYRGAGESLGLDARQCP
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCEEECEEEEECCCCCCCCCHHHCC
AEALLITTPWGAYLGDEALQQGVDVQVSSWRRNGAGAASTLAKIGGQYVNGQAIVMEAHE
CCEEEEECCCCHHCCHHHHHCCCCEEEECCCCCCCCHHHHHHHHCCEEECCEEEEEEECC
NGMSEGIALDTNGFVSEGSGENVFLVYKNEIFTPSIGSSILSGITRHCVIRIARDLGYTV
CCCCCCEEECCCCCEECCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEE
TETSIPREMLYLADEIFFTGTAVEICPVRSVDRMPVGSGKRGAVTKAIQDQYFGIVRGTH
ECCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHEEECCC
ADKWNWLTPVAVPSRDGARA
CCCCCCCCCEECCCCCCCCC
>Mature Secondary Structure
MRSATTMKSSYVWKNGELVPWEHAQVHVMSHALHYGTSVFEGVRAYEIGEKAAILCGREH
CCCCCCCHHHEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCHHHHHCCCHHEEEECHHH
FERLLFSCKVARIASPMSVGQWMEATVETLRANGQRSAYIRPLVYRGAGESLGLDARQCP
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCEEECEEEEECCCCCCCCCHHHCC
AEALLITTPWGAYLGDEALQQGVDVQVSSWRRNGAGAASTLAKIGGQYVNGQAIVMEAHE
CCEEEEECCCCHHCCHHHHHCCCCEEEECCCCCCCCHHHHHHHHCCEEECCEEEEEEECC
NGMSEGIALDTNGFVSEGSGENVFLVYKNEIFTPSIGSSILSGITRHCVIRIARDLGYTV
CCCCCCEEECCCCCEECCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEE
TETSIPREMLYLADEIFFTGTAVEICPVRSVDRMPVGSGKRGAVTKAIQDQYFGIVRGTH
ECCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHEEECCC
ADKWNWLTPVAVPSRDGARA
CCCCCCCCCEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9371463 [H]