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The map label for this gene is gcvA [H]

Identifier: 126444309

GI number: 126444309

Start: 818782

End: 819687

Strand: Reverse

Name: gcvA [H]

Synonym: BURPS668_A0840

Alternate gene names: 126444309

Gene position: 819687-818782 (Counterclockwise)

Preceding gene: 126444104

Following gene: 126442957

Centisome position: 26.21

GC content: 72.3

Gene sequence:

>906_bases
GTGCTTATGACGAGACCAGACCGTCTGCCGCCGATGCAGACCTTGTCCGCGTTCGAAGCGGCCGCGCGCCTCGCGAGCTT
CACGGCCGCCGCGCGCGAGCTCGGCTCGACGCAGCCGGCCGTCAGCCAGCGCGTCGTTCAGCTCGAAGAGGATCTCGGCA
CGCCGCTCTTCGAGCGCGGGCGCCGCGGCGTCACGCTGACAGAGGACGGCGCGCGGCTCTTCGCGGCGGTCCGCCAAAGC
CTCGACGCGCTGCGCGCCGCGACGGCGGACATCCGCAGCCGCCGCGCGAACGGCACATTCACACTCGTCACCGATTTCGG
CTTCGCCACCTACTGGCTGATGCCGCGCCTCGACGATCTGAAGCGCGCGATGCCCGGCGTCGACGTGCGCGTCGTCACAT
CTCAGGACATCGATCCGCAGCGCGAGCACGCCGACGTCGCGATCCTGTTCGGCGCCGGCGACTGGCCGGGCTGCACATCG
ACGCGGCTCTTTCAGGAGCACGTGACGCCCGTGTGCTCGCCCGCGTTTCGCACCGCGCATGCCGATATCGCGCGGCCGGC
CGACCTGTTGCGCGCGCCGCTGCTGCACGTGCAGCCGACGCGCCCCGAGCGCTGGCTCGCGTGGCGCGATTGGTTCGACG
CGCATGGGCTCGCCGCGCCGCCCGAGCCGCACGGGCTGACGTTCAACAGCTACTCGCTCGTGATTCAAGCGGCGCTGATG
AATCAGGGTGTCGCGCTCGGCTGGGCGCCGCTCGTCGACACGCCGATCGCGGCCGGCCAGCTCGTGCGGCTCGTCGACGC
GCCCGTCGTCACGCCGCGCGGCTACTACCTCGTTCTGCCGCCCGCGCGGCCGGAGGCGCGCGCGGTGCCGCTCTTTCGCC
GCTGGCTGCTCGGCGCATGCGCATGA

Upstream 100 bases:

>100_bases
TCGGAACGGCGGCGCGACAGGCTGCCGCCGCCGGCCGGATCTCACTATTGCCGGCCGTAATGCGGCCGTGAAGGCATCGA
ATGCTTATGAGCCATCAGAG

Downstream 100 bases:

>100_bases
CGCATCGGGCATTGGCGCGCCGATTCCAGCAGCGCGGCGATTCGGTTCTATCCGCATCCGATCTGGCTTGATTAACTGGC
GTATCGCCTCGGACGCCCGG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 301; Mature: 301

Protein sequence:

>301_residues
MLMTRPDRLPPMQTLSAFEAAARLASFTAAARELGSTQPAVSQRVVQLEEDLGTPLFERGRRGVTLTEDGARLFAAVRQS
LDALRAATADIRSRRANGTFTLVTDFGFATYWLMPRLDDLKRAMPGVDVRVVTSQDIDPQREHADVAILFGAGDWPGCTS
TRLFQEHVTPVCSPAFRTAHADIARPADLLRAPLLHVQPTRPERWLAWRDWFDAHGLAAPPEPHGLTFNSYSLVIQAALM
NQGVALGWAPLVDTPIAAGQLVRLVDAPVVTPRGYYLVLPPARPEARAVPLFRRWLLGACA

Sequences:

>Translated_301_residues
MLMTRPDRLPPMQTLSAFEAAARLASFTAAARELGSTQPAVSQRVVQLEEDLGTPLFERGRRGVTLTEDGARLFAAVRQS
LDALRAATADIRSRRANGTFTLVTDFGFATYWLMPRLDDLKRAMPGVDVRVVTSQDIDPQREHADVAILFGAGDWPGCTS
TRLFQEHVTPVCSPAFRTAHADIARPADLLRAPLLHVQPTRPERWLAWRDWFDAHGLAAPPEPHGLTFNSYSLVIQAALM
NQGVALGWAPLVDTPIAAGQLVRLVDAPVVTPRGYYLVLPPARPEARAVPLFRRWLLGACA
>Mature_301_residues
MLMTRPDRLPPMQTLSAFEAAARLASFTAAARELGSTQPAVSQRVVQLEEDLGTPLFERGRRGVTLTEDGARLFAAVRQS
LDALRAATADIRSRRANGTFTLVTDFGFATYWLMPRLDDLKRAMPGVDVRVVTSQDIDPQREHADVAILFGAGDWPGCTS
TRLFQEHVTPVCSPAFRTAHADIARPADLLRAPLLHVQPTRPERWLAWRDWFDAHGLAAPPEPHGLTFNSYSLVIQAALM
NQGVALGWAPLVDTPIAAGQLVRLVDAPVVTPRGYYLVLPPARPEARAVPLFRRWLLGACA

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=298, Percent_Identity=37.248322147651, Blast_Score=191, Evalue=4e-50,
Organism=Escherichia coli, GI1788706, Length=292, Percent_Identity=34.2465753424658, Blast_Score=153, Evalue=1e-38,
Organism=Escherichia coli, GI1786448, Length=295, Percent_Identity=30.5084745762712, Blast_Score=122, Evalue=2e-29,
Organism=Escherichia coli, GI1786401, Length=262, Percent_Identity=28.2442748091603, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI87081978, Length=269, Percent_Identity=28.996282527881, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI145693193, Length=304, Percent_Identity=25.9868421052632, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI145693105, Length=123, Percent_Identity=33.3333333333333, Blast_Score=68, Evalue=9e-13,
Organism=Escherichia coli, GI1787601, Length=126, Percent_Identity=34.1269841269841, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI157672245, Length=166, Percent_Identity=30.7228915662651, Blast_Score=65, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33057; Mature: 33057

Theoretical pI: Translated: 8.50; Mature: 8.50

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLMTRPDRLPPMQTLSAFEAAARLASFTAAARELGSTQPAVSQRVVQLEEDLGTPLFERG
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHHCC
RRGVTLTEDGARLFAAVRQSLDALRAATADIRSRRANGTFTLVTDFGFATYWLMPRLDDL
CCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHCCCHHHH
KRAMPGVDVRVVTSQDIDPQREHADVAILFGAGDWPGCTSTRLFQEHVTPVCSPAFRTAH
HHHCCCCCEEEEECCCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHCCHHCCHHHHHHH
ADIARPADLLRAPLLHVQPTRPERWLAWRDWFDAHGLAAPPEPHGLTFNSYSLVIQAALM
HHHCCCHHHHHCCCCEECCCCCHHHHHHHHHHCCCCCCCCCCCCCEEECHHHHHHHHHHH
NQGVALGWAPLVDTPIAAGQLVRLVDAPVVTPRGYYLVLPPARPEARAVPLFRRWLLGAC
HCCCEEEEHHHHCCCCHHHHHHHHHCCCEECCCCCEEEECCCCCCCCCCHHHHHHHHHCC
A
C
>Mature Secondary Structure
MLMTRPDRLPPMQTLSAFEAAARLASFTAAARELGSTQPAVSQRVVQLEEDLGTPLFERG
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHHCC
RRGVTLTEDGARLFAAVRQSLDALRAATADIRSRRANGTFTLVTDFGFATYWLMPRLDDL
CCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHCCCHHHH
KRAMPGVDVRVVTSQDIDPQREHADVAILFGAGDWPGCTSTRLFQEHVTPVCSPAFRTAH
HHHCCCCCEEEEECCCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHCCHHCCHHHHHHH
ADIARPADLLRAPLLHVQPTRPERWLAWRDWFDAHGLAAPPEPHGLTFNSYSLVIQAALM
HHHCCCHHHHHCCCCEECCCCCHHHHHHHHHHCCCCCCCCCCCCCEEECHHHHHHHHHHH
NQGVALGWAPLVDTPIAAGQLVRLVDAPVVTPRGYYLVLPPARPEARAVPLFRRWLLGAC
HCCCEEEEHHHHCCCCHHHHHHHHHCCCEECCCCCEEEECCCCCCCCCCHHHHHHHHHCC
A
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]