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The map label for this gene is rpfG [H]

Identifier: 126444221

GI number: 126444221

Start: 2255166

End: 2256593

Strand: Reverse

Name: rpfG [H]

Synonym: BURPS668_A2314

Alternate gene names: 126444221

Gene position: 2256593-2255166 (Counterclockwise)

Preceding gene: 126442725

Following gene: 126445421

Centisome position: 72.15

GC content: 68.91

Gene sequence:

>1428_bases
ATGAGCAAACGAGCGAAAGCGAGCAGTCCTTCGATATCGATACATAGCAACGGGCCCGCCGGGGAGGACGACGCGCGGCC
GGCGCATGCGGCGGGCGACGCGCCCGCCGATGCGCCGGTCGTGCTGCTCGTCGACGACGAGCCGAGCGTGCTGTCGGCGC
TCAAGCGCGTGTTTCGCCCGAAGCATTACCGGACGCTGTGCGCCGACAGCGGCGAAGCGGCGCTCGATCTGCTGGCCGCG
GGCGAAGTCGACCTGATCGTGTCCGATATGCGGATGCCGCGGATGAGCGGCGCGGAACTGCTCACGCGCGTGGGCGAACG
ACAGCCGGACACGATGCGCATTCTGCTGACCGGTTATTCCGAAATCGATTCGGCGGTGCGCGCGATCAACGAAGCGGGCA
TCTACCGGTACCTGACGAAGCCGTGGGACGATCACGACTTGCTGCTGACCGTCGAGCAGGCGCTCGAGCAGCAGCGCCTG
CGCCGCGAGACCGCCCGGCTCGACGCGCTCACGCGCAAGCAGAACGATGCGTTGCGCGCGTTCAACGCGGGGCTCGAAGC
GCAGGTGCAGGCGCGCGTCGAAGAGATCCGCCAGACGATGCTGTTCCTCGAAGACGCGCAACGCGACCTGACGCACAATT
TCGCCGCGATGGCGCAGGTGTGCGCGAACATGATCGAGCTGCGCTGCGGCGCGCCGGGCGGCGAGTCGCAACGCGTCGGC
GAGGCGGCGCGCGATCTGGCGCTCGCGCTCGGCATGAGCGGCTGGCAAGCGCTGGAGCTGTTCTTCGCGGGCCTGCTGCA
CGGAATCGGCAAGCTGTCGCTGCCGGACGAGCTGCTGCACCGGCCGCTCAACCGGCTGACGCCGGACGAGAGCCGCCTCT
ATTACCAGCACCCGCTGCGCGCGCAGATGGTGCTCACGCCGGTCCAGCAGCTCGGCCGCGTCGCGCACGTGATTCATCAC
CAATACGAGCGGTTCGACGGGCGCGGCACGCCCGACGGGCTTGCCGCCAACGCGATTCCGCTCGGCTCGCGCATTCTCGC
CGTCGCGCGCGATTTCGACGCGCTCTGTAGCGGCAGCATGTTCACACGGCCGTACACGGTGCAGCAGGCGATGGCGGTGC
TCGTCTCGCAGTCCGGCCTGCGCTACGACGCACGCGTCGTCGACCGATTCGTCGCGCTGTCGAAGGAAGCGGCGGCGGTC
GACCGGCGCGCGAGCGTATCGCGCATCAACGCTTCGCAACTCGCCGAAGGCATGCGGCTTGCCGACGACCTGCGCAACGG
CCGCGGCATCCTGCTGATGACGAAGGGCAGCGTCGTGTCGTCGCATCAGGCCGCGCAGGTGCGCCGCTTCGAGGCGAGCG
AAACGGCGCCGTTCGTCATTTTCGTCGATGCGGCGGGCGGCGAGCCGCCGCGCGGCATGTTGGACTGA

Upstream 100 bases:

>100_bases
CATTTCTGATGTTTTTCGCGTTTCCCACGTTTTCCCTAGTGCGCCCGAGCGCGCGGGCTGCCGATATCCCAAAAAAAGAG
AAATCGGCAATGAGGGCACC

Downstream 100 bases:

>100_bases
TACATCACGGCCGGCGACGGCGAACCGCCCCGTGCCCGGCCACGAAGACATCGGTTCGTTCGAAGCCGACTGATGAAGGG
GAGATCTGTCGTGACCATGT

Product: response regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 475; Mature: 474

Protein sequence:

>475_residues
MSKRAKASSPSISIHSNGPAGEDDARPAHAAGDAPADAPVVLLVDDEPSVLSALKRVFRPKHYRTLCADSGEAALDLLAA
GEVDLIVSDMRMPRMSGAELLTRVGERQPDTMRILLTGYSEIDSAVRAINEAGIYRYLTKPWDDHDLLLTVEQALEQQRL
RRETARLDALTRKQNDALRAFNAGLEAQVQARVEEIRQTMLFLEDAQRDLTHNFAAMAQVCANMIELRCGAPGGESQRVG
EAARDLALALGMSGWQALELFFAGLLHGIGKLSLPDELLHRPLNRLTPDESRLYYQHPLRAQMVLTPVQQLGRVAHVIHH
QYERFDGRGTPDGLAANAIPLGSRILAVARDFDALCSGSMFTRPYTVQQAMAVLVSQSGLRYDARVVDRFVALSKEAAAV
DRRASVSRINASQLAEGMRLADDLRNGRGILLMTKGSVVSSHQAAQVRRFEASETAPFVIFVDAAGGEPPRGMLD

Sequences:

>Translated_475_residues
MSKRAKASSPSISIHSNGPAGEDDARPAHAAGDAPADAPVVLLVDDEPSVLSALKRVFRPKHYRTLCADSGEAALDLLAA
GEVDLIVSDMRMPRMSGAELLTRVGERQPDTMRILLTGYSEIDSAVRAINEAGIYRYLTKPWDDHDLLLTVEQALEQQRL
RRETARLDALTRKQNDALRAFNAGLEAQVQARVEEIRQTMLFLEDAQRDLTHNFAAMAQVCANMIELRCGAPGGESQRVG
EAARDLALALGMSGWQALELFFAGLLHGIGKLSLPDELLHRPLNRLTPDESRLYYQHPLRAQMVLTPVQQLGRVAHVIHH
QYERFDGRGTPDGLAANAIPLGSRILAVARDFDALCSGSMFTRPYTVQQAMAVLVSQSGLRYDARVVDRFVALSKEAAAV
DRRASVSRINASQLAEGMRLADDLRNGRGILLMTKGSVVSSHQAAQVRRFEASETAPFVIFVDAAGGEPPRGMLD
>Mature_474_residues
SKRAKASSPSISIHSNGPAGEDDARPAHAAGDAPADAPVVLLVDDEPSVLSALKRVFRPKHYRTLCADSGEAALDLLAAG
EVDLIVSDMRMPRMSGAELLTRVGERQPDTMRILLTGYSEIDSAVRAINEAGIYRYLTKPWDDHDLLLTVEQALEQQRLR
RETARLDALTRKQNDALRAFNAGLEAQVQARVEEIRQTMLFLEDAQRDLTHNFAAMAQVCANMIELRCGAPGGESQRVGE
AARDLALALGMSGWQALELFFAGLLHGIGKLSLPDELLHRPLNRLTPDESRLYYQHPLRAQMVLTPVQQLGRVAHVIHHQ
YERFDGRGTPDGLAANAIPLGSRILAVARDFDALCSGSMFTRPYTVQQAMAVLVSQSGLRYDARVVDRFVALSKEAAAVD
RRASVSRINASQLAEGMRLADDLRNGRGILLMTKGSVVSSHQAAQVRRFEASETAPFVIFVDAAGGEPPRGMLD

Specific function: Positively regulates the synthesis of extracellular enzymes and extracellular polysaccharides. The cell-cell signaling system encoded by rpf genes is required for full virulence and is implicated in regulation of the formation and dispersal of biofilms [H

COG id: COG3437

COG function: function code KT; Response regulator containing a CheY-like receiver domain and an HD-GYP domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1788550, Length=127, Percent_Identity=36.2204724409449, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI1788905, Length=117, Percent_Identity=37.6068376068376, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI1790299, Length=126, Percent_Identity=34.1269841269841, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI1790437, Length=115, Percent_Identity=33.9130434782609, Blast_Score=66, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR003607
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 51913; Mature: 51782

Theoretical pI: Translated: 6.66; Mature: 6.66

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKRAKASSPSISIHSNGPAGEDDARPAHAAGDAPADAPVVLLVDDEPSVLSALKRVFRP
CCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHCC
KHYRTLCADSGEAALDLLAAGEVDLIVSDMRMPRMSGAELLTRVGERQPDTMRILLTGYS
CHHHHHHCCCCHHHHHHHHCCCHHEEEHHHCCCCCCHHHHHHHHCCCCCCEEEEEEECHH
EIDSAVRAINEAGIYRYLTKPWDDHDLLLTVEQALEQQRLRRETARLDALTRKQNDALRA
HHHHHHHHHHHHHHHHHHCCCCCCCHHEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FNAGLEAQVQARVEEIRQTMLFLEDAQRDLTHNFAAMAQVCANMIELRCGAPGGESQRVG
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHH
EAARDLALALGMSGWQALELFFAGLLHGIGKLSLPDELLHRPLNRLTPDESRLYYQHPLR
HHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCEEECCCCH
AQMVLTPVQQLGRVAHVIHHQYERFDGRGTPDGLAANAIPLGSRILAVARDFDALCSGSM
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCC
FTRPYTVQQAMAVLVSQSGLRYDARVVDRFVALSKEAAAVDRRASVSRINASQLAEGMRL
CCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
ADDLRNGRGILLMTKGSVVSSHQAAQVRRFEASETAPFVIFVDAAGGEPPRGMLD
HHHHCCCCEEEEEECCCCHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCC
>Mature Secondary Structure 
SKRAKASSPSISIHSNGPAGEDDARPAHAAGDAPADAPVVLLVDDEPSVLSALKRVFRP
CCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHCC
KHYRTLCADSGEAALDLLAAGEVDLIVSDMRMPRMSGAELLTRVGERQPDTMRILLTGYS
CHHHHHHCCCCHHHHHHHHCCCHHEEEHHHCCCCCCHHHHHHHHCCCCCCEEEEEEECHH
EIDSAVRAINEAGIYRYLTKPWDDHDLLLTVEQALEQQRLRRETARLDALTRKQNDALRA
HHHHHHHHHHHHHHHHHHCCCCCCCHHEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FNAGLEAQVQARVEEIRQTMLFLEDAQRDLTHNFAAMAQVCANMIELRCGAPGGESQRVG
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHH
EAARDLALALGMSGWQALELFFAGLLHGIGKLSLPDELLHRPLNRLTPDESRLYYQHPLR
HHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCEEECCCCH
AQMVLTPVQQLGRVAHVIHHQYERFDGRGTPDGLAANAIPLGSRILAVARDFDALCSGSM
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCC
FTRPYTVQQAMAVLVSQSGLRYDARVVDRFVALSKEAAAVDRRASVSRINASQLAEGMRL
CCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
ADDLRNGRGILLMTKGSVVSSHQAAQVRRFEASETAPFVIFVDAAGGEPPRGMLD
HHHHCCCCEEEEEECCCCHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11123673 [H]