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The map label for this gene is truA

Identifier: 126443551

GI number: 126443551

Start: 2365163

End: 2365975

Strand: Reverse

Name: truA

Synonym: BURPS668_A2447

Alternate gene names: 126443551

Gene position: 2365975-2365163 (Counterclockwise)

Preceding gene: 126442479

Following gene: 228993425

Centisome position: 75.65

GC content: 67.77

Gene sequence:

>813_bases
ATGAGAATCGCACTCGGCATTCAGTACGACGGCGCGGCGTTCTGCGGCTGGCAGTCGCAGCCCCACGGCAAGACGGTTCA
GGACGCGCTCGAGCGCTCGCTCGCGGAATTCGCGCAGACGTCGCTGCACACGACGGTCGCGGGCCGCACCGACACCGGCG
TGCACGGCCTCGGCCAGGTCGTCCATTTCGATACCGACCTCGACCGCGCGGATTTCTCATGGGTGCGCGGCACGAACGCG
TTCCTGCCGCCGACGGTCGCCGTGCAGTGGGCGAAGCCGATGCCGGATACGTTCCACGCGCGCTTCGCCGCTTTCGAGCG
CACCTACTACTACGCGCTGTACGTGCATCCGGTGCGCTCGCCGATGCTCGCGGGGCGCGCGGGCTGGGTCCACACGCCGC
TCGACGTCGACGCAATGCGCGAAGCGGCCGCGCATCTCGTCGGCGAGCACGATTTCTCGGCGTTTCGCTCGTCCGAGTGC
CAGGCGAAATCGCCCGTCAAGCATCTGTACCAGATCGGCATCCGCCCCGACGGCGATTTCATCCACTTCCGTTTCCGCGC
GAACGCGTTCCTGCACCACATGGTGCGCAATCTGATGGGCTGCCTCGTCGCGGTGGGGCGCGGCCGCTATCCGTCGTCGT
GGCTCGCCGAAGTGCTGGAGAGCCGCGATCGCGATTGCGCGGCGCCGACTTTCATGCCCGAAGGGCTCTATCTCGCGCAC
GTCGGCTATCCGGCCGAATTCGCGGTGCCGCCCGCACAACTCGGCAGCGTGCCGTGGAGCAGCGTGTGGGCCGATCTGGA
CGGACGAACATGA

Upstream 100 bases:

>100_bases
CCGGCGCGCGCACGCTGCTGCACGAAGTGATCGAAGCCGATCACGCCGACACGCGCGACGAAGCGCGCGTGATGCTCGCG
AAACTCGCCGATCTGTCGTG

Downstream 100 bases:

>100_bases
TGACCGATACACCCGATGCGACCCTTCCGGCCGACGCGCCGCCGAACGACGCGCCCGCCGCCGGCGCGCGCCGCGACACG
GGCGGCGACGGCAGTCGCGA

Product: tRNA pseudouridine synthase A

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I; tRNA-uridine isomerase I

Number of amino acids: Translated: 270; Mature: 270

Protein sequence:

>270_residues
MRIALGIQYDGAAFCGWQSQPHGKTVQDALERSLAEFAQTSLHTTVAGRTDTGVHGLGQVVHFDTDLDRADFSWVRGTNA
FLPPTVAVQWAKPMPDTFHARFAAFERTYYYALYVHPVRSPMLAGRAGWVHTPLDVDAMREAAAHLVGEHDFSAFRSSEC
QAKSPVKHLYQIGIRPDGDFIHFRFRANAFLHHMVRNLMGCLVAVGRGRYPSSWLAEVLESRDRDCAAPTFMPEGLYLAH
VGYPAEFAVPPAQLGSVPWSSVWADLDGRT

Sequences:

>Translated_270_residues
MRIALGIQYDGAAFCGWQSQPHGKTVQDALERSLAEFAQTSLHTTVAGRTDTGVHGLGQVVHFDTDLDRADFSWVRGTNA
FLPPTVAVQWAKPMPDTFHARFAAFERTYYYALYVHPVRSPMLAGRAGWVHTPLDVDAMREAAAHLVGEHDFSAFRSSEC
QAKSPVKHLYQIGIRPDGDFIHFRFRANAFLHHMVRNLMGCLVAVGRGRYPSSWLAEVLESRDRDCAAPTFMPEGLYLAH
VGYPAEFAVPPAQLGSVPWSSVWADLDGRT
>Mature_270_residues
MRIALGIQYDGAAFCGWQSQPHGKTVQDALERSLAEFAQTSLHTTVAGRTDTGVHGLGQVVHFDTDLDRADFSWVRGTNA
FLPPTVAVQWAKPMPDTFHARFAAFERTYYYALYVHPVRSPMLAGRAGWVHTPLDVDAMREAAAHLVGEHDFSAFRSSEC
QAKSPVKHLYQIGIRPDGDFIHFRFRANAFLHHMVRNLMGCLVAVGRGRYPSSWLAEVLESRDRDCAAPTFMPEGLYLAH
VGYPAEFAVPPAQLGSVPWSSVWADLDGRT

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family

Homologues:

Organism=Homo sapiens, GI23503259, Length=269, Percent_Identity=31.5985130111524, Blast_Score=95, Evalue=5e-20,
Organism=Escherichia coli, GI1788657, Length=254, Percent_Identity=44.8818897637795, Blast_Score=240, Evalue=7e-65,
Organism=Caenorhabditis elegans, GI71985046, Length=261, Percent_Identity=25.6704980842912, Blast_Score=73, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUA_BURM7 (A3MBU2)

Other databases:

- EMBL:   CP000547
- RefSeq:   YP_001077728.1
- ProteinModelPortal:   A3MBU2
- SMR:   A3MBU2
- GeneID:   4890669
- GenomeReviews:   CP000547_GR
- KEGG:   bmn:BMA10247_A0527
- TIGR:   BMA10247_A0527
- HOGENOM:   HBG621020
- OMA:   QSKTPFR
- ProtClustDB:   PRK00021
- BioCyc:   BMAL320389:BMA10247_A0527-MONOMER
- HAMAP:   MF_00171
- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094
- Gene3D:   G3DSA:3.30.70.580
- Gene3D:   G3DSA:3.30.70.660
- PANTHER:   PTHR11142
- PIRSF:   PIRSF001430
- TIGRFAMs:   TIGR00071

Pfam domain/function: PF01416 PseudoU_synth_1; SSF55120 PsdUridine_synth_cat_dom

EC number: =5.4.99.12

Molecular weight: Translated: 30014; Mature: 30014

Theoretical pI: Translated: 7.04; Mature: 7.04

Prosite motif: NA

Important sites: ACT_SITE 52-52

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIALGIQYDGAAFCGWQSQPHGKTVQDALERSLAEFAQTSLHTTVAGRTDTGVHGLGQV
CEEEEEEEECCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHEECCCCCCCCHHCCCE
VHFDTDLDRADFSWVRGTNAFLPPTVAVQWAKPMPDTFHARFAAFERTYYYALYVHPVRS
EEECCCCCCCCCHHHCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHEEEEEEEEECCCC
PMLAGRAGWVHTPLDVDAMREAAAHLVGEHDFSAFRSSECQAKSPVKHLYQIGIRPDGDF
CHHCCCCCCEECCCCHHHHHHHHHHHHCCCHHHHHHCCCCCCCCHHHHHHHHCCCCCCCE
IHFRFRANAFLHHMVRNLMGCLVAVGRGRYPSSWLAEVLESRDRDCAAPTFMPEGLYLAH
EEEEEEHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCEEEEE
VGYPAEFAVPPAQLGSVPWSSVWADLDGRT
ECCCCCCCCCHHHHCCCCHHHHHCCCCCCC
>Mature Secondary Structure
MRIALGIQYDGAAFCGWQSQPHGKTVQDALERSLAEFAQTSLHTTVAGRTDTGVHGLGQV
CEEEEEEEECCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHEECCCCCCCCHHCCCE
VHFDTDLDRADFSWVRGTNAFLPPTVAVQWAKPMPDTFHARFAAFERTYYYALYVHPVRS
EEECCCCCCCCCHHHCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHEEEEEEEEECCCC
PMLAGRAGWVHTPLDVDAMREAAAHLVGEHDFSAFRSSECQAKSPVKHLYQIGIRPDGDF
CHHCCCCCCEECCCCHHHHHHHHHHHHCCCHHHHHHCCCCCCCCHHHHHHHHCCCCCCCE
IHFRFRANAFLHHMVRNLMGCLVAVGRGRYPSSWLAEVLESRDRDCAAPTFMPEGLYLAH
EEEEEEHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCEEEEE
VGYPAEFAVPPAQLGSVPWSSVWADLDGRT
ECCCCCCCCCHHHHCCCCHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA