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The map label for this gene is fadD28 [H]

Identifier: 126443518

GI number: 126443518

Start: 1759787

End: 1761619

Strand: Reverse

Name: fadD28 [H]

Synonym: BURPS668_A1800

Alternate gene names: 126443518

Gene position: 1761619-1759787 (Counterclockwise)

Preceding gene: 126443801

Following gene: 126442363

Centisome position: 56.33

GC content: 69.18

Gene sequence:

>1833_bases
GTGCGCCGTGTACAAATTACTGATGGAGCAATCGTGCCGTCTAATTTTGCCGAACACTTCGAAAATCAAGTCTTGAAGTA
CGGGAATCGCAGTCAATTTGTTTTTCTACACGGAAAACCCGGTGATTCGGCCGGCGAGGCGCGGCTCGGCTATGCGCAAC
TGGATGCCCGCGCGCGGTCGATCGCCGCGTGGCTGTCGGCGTGCGGCGCCGCGTGCCGGCCGGTTCTGCTCGTCTATCCC
GAAGGCCTCGCGTTTGTGGCGGCTTTTCTCGGCTGCCTGTATGCCGGCGCGATCGCCGTGCCGGTGCCCGTGCCCGCGGA
CGCGCGCAGCGTCGAACGAATCAGGCGGATCGTCCGCGATGCGGGCATCGCGCTTGCGCTGACGCCGACGGCCGCCGATG
CGCACGCGCTCGCGAACTGCCTGGCCGCGCCCGGCGATGCGGTCTCCACGGTCGGCGCGCTGCGATGCGAGGCCACCGAC
GCGCTCGAGCCCCTCGATGCGAGCGCGTGGAGCAGGCCGGCGCTCGATGCCTCGGCGATCGCGTTCTTGCAATACACGTC
CGGCTCGACGGGAGAGCCCAAAGGCGTCGTCGTGTCGCATCGTAATCTGCTGCACAACGAAGCGCAGATTCAGCGTGTCG
TGGCGAGCGACGCCGATAGCGTCGTGACGGGATGGCTGCCGCATTTTCACGACATGGGGCTGATCGGCATGCTGCTGCAT
CCGCTGTACGTCGGCTGCGACTGCGTGTTCATGGCGCCGTCCACGTTCTTGCGGCGTCCCGTTCGATGGCTGGAGGCCGC
GACGCATTACGCGGCCACGATTACGGTTGCGCCGAACTTCGCGTACGACCTGTGCGCGCGCGCCGTGAAGCGCGAGCAAC
TCGGCGGGCTCGATCTGTCCGCGCTGAAGGTCGCGTTGAACGGCGCGGAGCCGGTTCGCGCGAAAACGATCGAAGCGTTC
TCGGCGCGTTTCGCGCCGGTCGGCTTCGATGCACGGGCGTTTTCGCCGTGCTACGGGATGGCCGAGGTCACGTTGCTCGC
GAGCGCGGGCCGCCCGGCGGCGGCGCCCGTGCTGCTCGATATCGACGCGCGGGCGCTCGAGCGCCATGAGGCATCGTTGG
CGAAGCCCGGTGCCGAGGCGTTGCGCGTCGTCGGCAGTGGGCGCGCCGTCGATCTCGATCTGCGCATCGTCGATCCGCAA
ACGCGCGCCGTGGCGGCCGACGGGCGCATCGGCGAGATCTGGCTGCGCGGCGACAGCGTCGCGCAAGGCTACTGGCGCCG
CCCCGACGCGAGCGAGGCGGCGTTCCGGGCGCGCACGGCCGCAGGCGACGGGCCGTATCTGCGGACCGGCGATCTCGGTT
TCGTGCGGGACGGGGAGCTGTTCGTGACCGGGCGCCTCAAGGACCTGCTGATCGTCAACGGCCGCAACCTCTATCCGCAG
GACATCGAGGATTTCGTTCGCGAGTTGCATCCCGCGCTCGGCGCGGGCGCGGGCGCGGCGTTTTCGATCGATGCGGACGG
CGAACGCGTCGTCGTCGTCCATGAAATCAAGGAGGCGCGGCTCGACGATGGCCTTTCGGCCGCCGCGCTGTCCGCTCGCA
TCAGGACGGCGCTCGCGCGTGCGTTCGACATCGCGGCCCCGAGCGTCGTGCTCGTGCCGCGCGGCGGCGTGAAGCGCACG
ACGAGCGGCAAGGTGCAGCGCGGCCTCACGCGCGACGCGTTTCTGGCCGCGCGGCTGCGCGTGCTGCACGAGGATATCCA
GGTGCCGGTTCGGTCGTTGCGTGTGGCCGCGGGCGAGGGCGTCGCGCAGGATGCCTCCCGGGGCGCCGCATGA

Upstream 100 bases:

>100_bases
TGCACAATGGTTGGGCATGGCGATGCGTGAAATGCGGCGCGTGAATTGCATTTTCTAAATGATTGTTCAGGGTTCCAGTG
CCGGAATGAATATCGCTTGA

Downstream 100 bases:

>100_bases
GCCTGAACCATGCGCCGTTCGCGCTCGCGAGCGCGTTCGACCGTTACCTCGGCGATCCGTCGATGCCCGACAACGCGTTT
TCATACGCGCGATGCGCGCA

Product: AMP-binding domain-containing protein

Products: AMP; diphosphate +an acyl-CoA [C]

Alternate protein names: Acyl-CoA synthetase [H]

Number of amino acids: Translated: 610; Mature: 610

Protein sequence:

>610_residues
MRRVQITDGAIVPSNFAEHFENQVLKYGNRSQFVFLHGKPGDSAGEARLGYAQLDARARSIAAWLSACGAACRPVLLVYP
EGLAFVAAFLGCLYAGAIAVPVPVPADARSVERIRRIVRDAGIALALTPTAADAHALANCLAAPGDAVSTVGALRCEATD
ALEPLDASAWSRPALDASAIAFLQYTSGSTGEPKGVVVSHRNLLHNEAQIQRVVASDADSVVTGWLPHFHDMGLIGMLLH
PLYVGCDCVFMAPSTFLRRPVRWLEAATHYAATITVAPNFAYDLCARAVKREQLGGLDLSALKVALNGAEPVRAKTIEAF
SARFAPVGFDARAFSPCYGMAEVTLLASAGRPAAAPVLLDIDARALERHEASLAKPGAEALRVVGSGRAVDLDLRIVDPQ
TRAVAADGRIGEIWLRGDSVAQGYWRRPDASEAAFRARTAAGDGPYLRTGDLGFVRDGELFVTGRLKDLLIVNGRNLYPQ
DIEDFVRELHPALGAGAGAAFSIDADGERVVVVHEIKEARLDDGLSAAALSARIRTALARAFDIAAPSVVLVPRGGVKRT
TSGKVQRGLTRDAFLAARLRVLHEDIQVPVRSLRVAAGEGVAQDASRGAA

Sequences:

>Translated_610_residues
MRRVQITDGAIVPSNFAEHFENQVLKYGNRSQFVFLHGKPGDSAGEARLGYAQLDARARSIAAWLSACGAACRPVLLVYP
EGLAFVAAFLGCLYAGAIAVPVPVPADARSVERIRRIVRDAGIALALTPTAADAHALANCLAAPGDAVSTVGALRCEATD
ALEPLDASAWSRPALDASAIAFLQYTSGSTGEPKGVVVSHRNLLHNEAQIQRVVASDADSVVTGWLPHFHDMGLIGMLLH
PLYVGCDCVFMAPSTFLRRPVRWLEAATHYAATITVAPNFAYDLCARAVKREQLGGLDLSALKVALNGAEPVRAKTIEAF
SARFAPVGFDARAFSPCYGMAEVTLLASAGRPAAAPVLLDIDARALERHEASLAKPGAEALRVVGSGRAVDLDLRIVDPQ
TRAVAADGRIGEIWLRGDSVAQGYWRRPDASEAAFRARTAAGDGPYLRTGDLGFVRDGELFVTGRLKDLLIVNGRNLYPQ
DIEDFVRELHPALGAGAGAAFSIDADGERVVVVHEIKEARLDDGLSAAALSARIRTALARAFDIAAPSVVLVPRGGVKRT
TSGKVQRGLTRDAFLAARLRVLHEDIQVPVRSLRVAAGEGVAQDASRGAA
>Mature_610_residues
MRRVQITDGAIVPSNFAEHFENQVLKYGNRSQFVFLHGKPGDSAGEARLGYAQLDARARSIAAWLSACGAACRPVLLVYP
EGLAFVAAFLGCLYAGAIAVPVPVPADARSVERIRRIVRDAGIALALTPTAADAHALANCLAAPGDAVSTVGALRCEATD
ALEPLDASAWSRPALDASAIAFLQYTSGSTGEPKGVVVSHRNLLHNEAQIQRVVASDADSVVTGWLPHFHDMGLIGMLLH
PLYVGCDCVFMAPSTFLRRPVRWLEAATHYAATITVAPNFAYDLCARAVKREQLGGLDLSALKVALNGAEPVRAKTIEAF
SARFAPVGFDARAFSPCYGMAEVTLLASAGRPAAAPVLLDIDARALERHEASLAKPGAEALRVVGSGRAVDLDLRIVDPQ
TRAVAADGRIGEIWLRGDSVAQGYWRRPDASEAAFRARTAAGDGPYLRTGDLGFVRDGELFVTGRLKDLLIVNGRNLYPQ
DIEDFVRELHPALGAGAGAAFSIDADGERVVVVHEIKEARLDDGLSAAALSARIRTALARAFDIAAPSVVLVPRGGVKRT
TSGKVQRGLTRDAFLAARLRVLHEDIQVPVRSLRVAAGEGVAQDASRGAA

Specific function: Esterification, Concomitant With Transport, Of Exogenous Long-Chain Fatty Acids Into Metabolically Active CoA Thioesters For Subsequent Degradation Or Incorporation Into Phospholipids. [C]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI55749758, Length=469, Percent_Identity=27.5053304904051, Blast_Score=124, Evalue=2e-28,
Organism=Homo sapiens, GI44888818, Length=568, Percent_Identity=26.9366197183099, Blast_Score=122, Evalue=8e-28,
Organism=Homo sapiens, GI45827692, Length=565, Percent_Identity=27.6106194690265, Blast_Score=121, Evalue=2e-27,
Organism=Homo sapiens, GI225735629, Length=565, Percent_Identity=27.6106194690265, Blast_Score=121, Evalue=2e-27,
Organism=Homo sapiens, GI225735625, Length=596, Percent_Identity=22.4832214765101, Blast_Score=83, Evalue=6e-16,
Organism=Homo sapiens, GI45827694, Length=530, Percent_Identity=23.0188679245283, Blast_Score=76, Evalue=8e-14,
Organism=Homo sapiens, GI45827696, Length=494, Percent_Identity=22.8744939271255, Blast_Score=75, Evalue=2e-13,
Organism=Homo sapiens, GI225735627, Length=494, Percent_Identity=22.8744939271255, Blast_Score=75, Evalue=2e-13,
Organism=Escherichia coli, GI1788107, Length=599, Percent_Identity=25.7095158597663, Blast_Score=120, Evalue=3e-28,
Organism=Escherichia coli, GI145693145, Length=558, Percent_Identity=24.0143369175627, Blast_Score=80, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI193202679, Length=597, Percent_Identity=24.6231155778894, Blast_Score=122, Evalue=4e-28,
Organism=Caenorhabditis elegans, GI17558820, Length=508, Percent_Identity=25.5905511811024, Blast_Score=103, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17531443, Length=557, Percent_Identity=23.3393177737882, Blast_Score=95, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17559526, Length=327, Percent_Identity=27.5229357798165, Blast_Score=93, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI17560308, Length=311, Percent_Identity=25.08038585209, Blast_Score=70, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24654656, Length=473, Percent_Identity=24.9471458773784, Blast_Score=109, Evalue=5e-24,
Organism=Drosophila melanogaster, GI18859661, Length=407, Percent_Identity=26.2899262899263, Blast_Score=109, Evalue=5e-24,
Organism=Drosophila melanogaster, GI24581924, Length=400, Percent_Identity=25.5, Blast_Score=92, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 6.2.1.3 [C]

Molecular weight: Translated: 64597; Mature: 64597

Theoretical pI: Translated: 7.78; Mature: 7.78

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRVQITDGAIVPSNFAEHFENQVLKYGNRSQFVFLHGKPGDSAGEARLGYAQLDARARS
CCEEEECCCCCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHH
IAAWLSACGAACRPVLLVYPEGLAFVAAFLGCLYAGAIAVPVPVPADARSVERIRRIVRD
HHHHHHHHHHHHCCEEEECCCHHHHHHHHHHHHHHCCEEECCCCCCCHHHHHHHHHHHHH
AGIALALTPTAADAHALANCLAAPGDAVSTVGALRCEATDALEPLDASAWSRPALDASAI
CCEEEEEECCCCHHHHHHHHHCCCCHHHHHHHHHEECCCCCCCCCCCCCCCCCCCCHHHE
AFLQYTSGSTGEPKGVVVSHRNLLHNEAQIQRVVASDADSVVTGWLPHFHDMGLIGMLLH
EEEEECCCCCCCCCCEEEECHHHHHHHHHHHHHHHCCCCHHHHHCCCHHHCCCHHHHHHH
PLYVGCDCVFMAPSTFLRRPVRWLEAATHYAATITVAPNFAYDLCARAVKREQLGGLDLS
HHHHCCCEEEECCHHHHHHHHHHHHHHHHEEEEEEECCCHHHHHHHHHHHHHHHCCCCHH
ALKVALNGAEPVRAKTIEAFSARFAPVGFDARAFSPCYGMAEVTLLASAGRPAAAPVLLD
HHHEEECCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCHHEEEEEECCCCCCCCCEEEE
IDARALERHEASLAKPGAEALRVVGSGRAVDLDLRIVDPQTRAVAADGRIGEIWLRGDSV
CCHHHHHHHHHHHCCCCHHHHHHHCCCCEEEEEEEEECCCCCEEECCCCEEEEEEECCCH
AQGYWRRPDASEAAFRARTAAGDGPYLRTGDLGFVRDGELFVTGRLKDLLIVNGRNLYPQ
HCCCCCCCCCHHHHHHHHCCCCCCCEEEECCCCEEECCCEEEEECCEEEEEECCCCCCHH
DIEDFVRELHPALGAGAGAAFSIDADGERVVVVHEIKEARLDDGLSAAALSARIRTALAR
HHHHHHHHHHHHHCCCCCCEEEECCCCCEEEEEEECHHHHCCCCCHHHHHHHHHHHHHHH
AFDIAAPSVVLVPRGGVKRTTSGKVQRGLTRDAFLAARLRVLHEDIQVPVRSLRVAAGEG
HHHCCCCCEEEECCCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHCCHHHHHHHCCCC
VAQDASRGAA
CHHHHCCCCC
>Mature Secondary Structure
MRRVQITDGAIVPSNFAEHFENQVLKYGNRSQFVFLHGKPGDSAGEARLGYAQLDARARS
CCEEEECCCCCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHH
IAAWLSACGAACRPVLLVYPEGLAFVAAFLGCLYAGAIAVPVPVPADARSVERIRRIVRD
HHHHHHHHHHHHCCEEEECCCHHHHHHHHHHHHHHCCEEECCCCCCCHHHHHHHHHHHHH
AGIALALTPTAADAHALANCLAAPGDAVSTVGALRCEATDALEPLDASAWSRPALDASAI
CCEEEEEECCCCHHHHHHHHHCCCCHHHHHHHHHEECCCCCCCCCCCCCCCCCCCCHHHE
AFLQYTSGSTGEPKGVVVSHRNLLHNEAQIQRVVASDADSVVTGWLPHFHDMGLIGMLLH
EEEEECCCCCCCCCCEEEECHHHHHHHHHHHHHHHCCCCHHHHHCCCHHHCCCHHHHHHH
PLYVGCDCVFMAPSTFLRRPVRWLEAATHYAATITVAPNFAYDLCARAVKREQLGGLDLS
HHHHCCCEEEECCHHHHHHHHHHHHHHHHEEEEEEECCCHHHHHHHHHHHHHHHCCCCHH
ALKVALNGAEPVRAKTIEAFSARFAPVGFDARAFSPCYGMAEVTLLASAGRPAAAPVLLD
HHHEEECCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCHHEEEEEECCCCCCCCCEEEE
IDARALERHEASLAKPGAEALRVVGSGRAVDLDLRIVDPQTRAVAADGRIGEIWLRGDSV
CCHHHHHHHHHHHCCCCHHHHHHHCCCCEEEEEEEEECCCCCEEECCCCEEEEEEECCCH
AQGYWRRPDASEAAFRARTAAGDGPYLRTGDLGFVRDGELFVTGRLKDLLIVNGRNLYPQ
HCCCCCCCCCHHHHHHHHCCCCCCCEEEECCCCEEECCCEEEEECCEEEEEECCCCCCHH
DIEDFVRELHPALGAGAGAAFSIDADGERVVVVHEIKEARLDDGLSAAALSARIRTALAR
HHHHHHHHHHHHHCCCCCCEEEECCCCCEEEEEEECHHHHCCCCCHHHHHHHHHHHHHHH
AFDIAAPSVVLVPRGGVKRTTSGKVQRGLTRDAFLAARLRVLHEDIQVPVRSLRVAAGEG
HHHCCCCCEEEECCCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHCCHHHHHHHCCCC
VAQDASRGAA
CHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Salts [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0408 {octanoate}} 0.0125 {octanoate}} 0.0059 {octanoate}} 0.00072 {octanoate}} 0.0013 {undecanoate}} 0.0022 {pentadecanoate}} 0.053 {CoA}} 0.035 {CoA}} 0.034 {CoA}} 0.0034 {tridecanoate}} 0.002 {laurate}} 0.0019 {laurate}} 0.0

Substrates: ATP; a long-chain carboxylic acid; CoA [C]

Specific reaction: ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate +an acyl-CoA [C]

General reaction: Acid-thiol ligation; Phosphorylation [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12788972; 1527058 [H]