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The map label for this gene is pksG [H]

Identifier: 126443435

GI number: 126443435

Start: 1369202

End: 1370461

Strand: Reverse

Name: pksG [H]

Synonym: BURPS668_A1470

Alternate gene names: 126443435

Gene position: 1370461-1369202 (Counterclockwise)

Preceding gene: 126445245

Following gene: 126442412

Centisome position: 43.82

GC content: 64.13

Gene sequence:

>1260_bases
ATGACAGCCGTTGGTATTGAGGCGCTCAACGTTTATGCCGGGGTTGCCAGTCTCGACGTCTCGAGGCTGGCGGAGCATCG
CAAGCTGGACATGGCGAGATTCCAGAACCTGCTGATGCGGGAAAAATCGGTTGCGCTGCCCTATGAGGATCCGATCACCT
ATGGCGTCAACGCCGCCAAGCCGATCGTCGACGCGCTGACGCCCGACGAGCGCGACCGCATCGAAATGCTGATCACCTGC
ACCGAATCGGCATTCGATTTCGGCAAATCGATGAGCACCTACTTTCACCACCATCTCGGCCTGAAGCGCAACTGCCGCCT
GTTCGAAGTCAAGAACGCGTGCTACTCCGGCGTCGCCGGCCTGCAAACGGCGATCAACTTCATCCTGGCCCAGGTCTCGC
CCGGCGCGAAGGCGCTCGTGATCGCGACCGACCTGTCGCGCTTCATCGTCGAGGAAGGCGGCGAGGCCTTGTCCGCCGAC
TGGTCGTTCGCCGAGCCGAGCAGCGGCGCGGGCGCGGTCGCGATGCTCGTCAGCGACACGCCGCACGTGTTTCGCATCGA
CGTCGGCGCGAACGGGTACTACGGCTACGAGGTGATGGACACCTGCCGGCCGACCACCGATAGCGAAGCCGGCAATTCGG
ATCTGTCGCTCCTGTCGTATCTCGACTGCTGCGAAAACGCGTTCCTCGAATACCAGAAGCGCGTGTGCGACGTCGATTAC
GCGAGCACGTTCGGATTCCTCGCTTTTCACACGCCGTTCGGCGGCATGGTGAAGGGCGCGCACCGCAATCTGATGCGCAA
GGCGAGCCGCTGCTCGACGCAGGAAATCGAGCAGGACTTCCAGCGCCGCGCGGGCCCCGGGCTCGTCTACTGCCAGCGGC
TCGGCAACATCATGGGTGCGACGGCGATGCTGTCGGTCGCCAGCACGATCGACAACGGCGAGTATCGCGCGCCGCAGCGC
GTGGGCGTGTTCTCGTACGGCTCGGGCTGCTGCTCGGAGTTCTTCAGCGGCATCGTCGACGAGGAAGGCCAGCGCCGGCT
GCGCGGCATGCGCATCGGCGAGCAGTTGGACCGCCGCTACGCGCTGTCCATCGACGAATACGAGCACGTGCTCAAGGAAA
GCCGGGTCGTGCGCTTCGGCACCCGCAACGCGAAAATCGACGACGGCTTCATCCCCGCGGCGCGGCGCGCGCACGGCCGC
GAAACGCTCTTCCTGAGCCGGATCAACGAATACCATCGGGAATACGAATGGATATGCTGA

Upstream 100 bases:

>100_bases
AAAAATAATAGCTCCGATAGGGACTTAATTCCTTCACAATCCCTGCCCGGTTTTTATTGCGCACCAATTTAACGTTCCGC
GCAGCAAGGAGAATGACCTA

Downstream 100 bases:

>100_bases
GCCGGCCCGCCGACTACCGGACCATCCGCGTCCGGTACGACGGCGACATCTGCTTCCTGCAGCTTCATCGCCCCGACGCG
CAGAACGCGATCAACAACCG

Product: putative polyketide biosynthesis protein pksG

Products: acetoacetyl-ACP; Coenzyme A; CO2; acetyl-ACP [C]

Alternate protein names: HMG synthase [H]

Number of amino acids: Translated: 419; Mature: 418

Protein sequence:

>419_residues
MTAVGIEALNVYAGVASLDVSRLAEHRKLDMARFQNLLMREKSVALPYEDPITYGVNAAKPIVDALTPDERDRIEMLITC
TESAFDFGKSMSTYFHHHLGLKRNCRLFEVKNACYSGVAGLQTAINFILAQVSPGAKALVIATDLSRFIVEEGGEALSAD
WSFAEPSSGAGAVAMLVSDTPHVFRIDVGANGYYGYEVMDTCRPTTDSEAGNSDLSLLSYLDCCENAFLEYQKRVCDVDY
ASTFGFLAFHTPFGGMVKGAHRNLMRKASRCSTQEIEQDFQRRAGPGLVYCQRLGNIMGATAMLSVASTIDNGEYRAPQR
VGVFSYGSGCCSEFFSGIVDEEGQRRLRGMRIGEQLDRRYALSIDEYEHVLKESRVVRFGTRNAKIDDGFIPAARRAHGR
ETLFLSRINEYHREYEWIC

Sequences:

>Translated_419_residues
MTAVGIEALNVYAGVASLDVSRLAEHRKLDMARFQNLLMREKSVALPYEDPITYGVNAAKPIVDALTPDERDRIEMLITC
TESAFDFGKSMSTYFHHHLGLKRNCRLFEVKNACYSGVAGLQTAINFILAQVSPGAKALVIATDLSRFIVEEGGEALSAD
WSFAEPSSGAGAVAMLVSDTPHVFRIDVGANGYYGYEVMDTCRPTTDSEAGNSDLSLLSYLDCCENAFLEYQKRVCDVDY
ASTFGFLAFHTPFGGMVKGAHRNLMRKASRCSTQEIEQDFQRRAGPGLVYCQRLGNIMGATAMLSVASTIDNGEYRAPQR
VGVFSYGSGCCSEFFSGIVDEEGQRRLRGMRIGEQLDRRYALSIDEYEHVLKESRVVRFGTRNAKIDDGFIPAARRAHGR
ETLFLSRINEYHREYEWIC
>Mature_418_residues
TAVGIEALNVYAGVASLDVSRLAEHRKLDMARFQNLLMREKSVALPYEDPITYGVNAAKPIVDALTPDERDRIEMLITCT
ESAFDFGKSMSTYFHHHLGLKRNCRLFEVKNACYSGVAGLQTAINFILAQVSPGAKALVIATDLSRFIVEEGGEALSADW
SFAEPSSGAGAVAMLVSDTPHVFRIDVGANGYYGYEVMDTCRPTTDSEAGNSDLSLLSYLDCCENAFLEYQKRVCDVDYA
STFGFLAFHTPFGGMVKGAHRNLMRKASRCSTQEIEQDFQRRAGPGLVYCQRLGNIMGATAMLSVASTIDNGEYRAPQRV
GVFSYGSGCCSEFFSGIVDEEGQRRLRGMRIGEQLDRRYALSIDEYEHVLKESRVVRFGTRNAKIDDGFIPAARRAHGRE
TLFLSRINEYHREYEWIC

Specific function: Involved in some intermediate steps for the synthesis of the antibiotic polyketide bacillaene which is involved in secondary metabolism. It catalyzes the aldol condensation between the acetyl group attached to the acyl-carrier-protein AcpK (Ac- AcpK) and

COG id: COG3425

COG function: function code I; 3-hydroxy-3-methylglutaryl CoA synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HMG-CoA synthase family [H]

Homologues:

Organism=Homo sapiens, GI148298764, Length=470, Percent_Identity=23.4042553191489, Blast_Score=82, Evalue=1e-15,
Organism=Homo sapiens, GI148298677, Length=470, Percent_Identity=23.4042553191489, Blast_Score=82, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6323509, Length=462, Percent_Identity=22.5108225108225, Blast_Score=84, Evalue=4e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013746
- InterPro:   IPR013528
- InterPro:   IPR016039
- InterPro:   IPR016038 [H]

Pfam domain/function: PF08540 HMG_CoA_synt_C; PF01154 HMG_CoA_synt_N [H]

EC number: 2.3.1.41 [C]

Molecular weight: Translated: 46589; Mature: 46458

Theoretical pI: Translated: 6.02; Mature: 6.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAVGIEALNVYAGVASLDVSRLAEHRKLDMARFQNLLMREKSVALPYEDPITYGVNAAK
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHH
PIVDALTPDERDRIEMLITCTESAFDFGKSMSTYFHHHLGLKRNCRLFEVKNACYSGVAG
HHHHHCCCCCCCCEEEEEEECHHHHHHHHHHHHHHHHHCCCCCCCEEEEEHHHHHHHHHH
LQTAINFILAQVSPGAKALVIATDLSRFIVEEGGEALSADWSFAEPSSGAGAVAMLVSDT
HHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHCCCCEEECCCCCCCCCCCCCEEEEEECCC
PHVFRIDVGANGYYGYEVMDTCRPTTDSEAGNSDLSLLSYLDCCENAFLEYQKRVCDVDY
CCEEEEEECCCCEECHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCH
ASTFGFLAFHTPFGGMVKGAHRNLMRKASRCSTQEIEQDFQRRAGPGLVYCQRLGNIMGA
HHHHCHHEEECCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHH
TAMLSVASTIDNGEYRAPQRVGVFSYGSGCCSEFFSGIVDEEGQRRLRGMRIGEQLDRRY
HHHHHHHHHHCCCCCCCCCCCCEECCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
ALSIDEYEHVLKESRVVRFGTRNAKIDDGFIPAARRAHGRETLFLSRINEYHREYEWIC
CCCHHHHHHHHHHCCEEEECCCCCCCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TAVGIEALNVYAGVASLDVSRLAEHRKLDMARFQNLLMREKSVALPYEDPITYGVNAAK
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHH
PIVDALTPDERDRIEMLITCTESAFDFGKSMSTYFHHHLGLKRNCRLFEVKNACYSGVAG
HHHHHCCCCCCCCEEEEEEECHHHHHHHHHHHHHHHHHCCCCCCCEEEEEHHHHHHHHHH
LQTAINFILAQVSPGAKALVIATDLSRFIVEEGGEALSADWSFAEPSSGAGAVAMLVSDT
HHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHCCCCEEECCCCCCCCCCCCCEEEEEECCC
PHVFRIDVGANGYYGYEVMDTCRPTTDSEAGNSDLSLLSYLDCCENAFLEYQKRVCDVDY
CCEEEEEECCCCEECHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCH
ASTFGFLAFHTPFGGMVKGAHRNLMRKASRCSTQEIEQDFQRRAGPGLVYCQRLGNIMGA
HHHHCHHEEECCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHH
TAMLSVASTIDNGEYRAPQRVGVFSYGSGCCSEFFSGIVDEEGQRRLRGMRIGEQLDRRY
HHHHHHHHHHCCCCCCCCCCCCEECCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
ALSIDEYEHVLKESRVVRFGTRNAKIDDGFIPAARRAHGRETLFLSRINEYHREYEWIC
CCCHHHHHHHHHHCCEEEECCCCCCCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: 2-Mercaptoethanol; DTT; EDTA [C]

Metal ions: Phosphate [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.04 {acetyl-ACP}} 0.031 {acetyl-ACP}} 0.138 {cis-9-hexadecenoyl-ACP}} 0.017 {cis-9-hexadecenoyl-ACP}} 0.012 {cis-3-decenoyl-ACP}} [C]

Substrates: acetyl-CoA; malonyl-ACP; acetyl-CoA [C]

Specific reaction: acetyl-CoA + malonyl-ACP = acetoacetyl-ACP + Coenzyme A + CO2 [acyl carrier protein] + acetyl-CoA = acetyl-ACP + Coenzyme A [C]

General reaction: Acyl group transfer [C]

Inhibitor: Acetyl-CoA; Acyl-CoA; Iodoacetamide; N-Ethyl maleimide acyl -ACP-derivatives protect, malonyl -ACP-decarboxyl ation, [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7704258; 9384377 [H]