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The map label for this gene is bcsZ [H]

Identifier: 126443038

GI number: 126443038

Start: 2163852

End: 2165372

Strand: Reverse

Name: bcsZ [H]

Synonym: BURPS668_A2231

Alternate gene names: 126443038

Gene position: 2165372-2163852 (Counterclockwise)

Preceding gene: 126443746

Following gene: 126444480

Centisome position: 69.24

GC content: 76.53

Gene sequence:

>1521_bases
ATGGCGCGGCAAGCGGGGCGAGACGAAGGCGTCGGGCGCATCGGGCGTGAAGCGGGCGACGCGCGCGAGGCGGCCGGGGC
GGGCGAGGCGTGCGGGAGGCGCCACGCGCGGGCCGACGCGTGGGCGCGGCGGGCGTCGCGAGCATCGCGCGGGTGGCGCA
CGTTTTGGCGCGGGCGCGGGGGCGTGCGCGCCGTCGTGGCGAGCGTCGTGGCGAGCTTCTCGGTGATGGCGTTCGCGGCG
GCGACGTTGCCGGTGTCGTGGCGCGTCGCCGCGGCGGCGGAGCGTACGCGAAGCGGCGGCGAACGCGCGGGCGGGCTGCG
CGACACGGCGGGCTTGATCGAGATCTCGGCGGCGGCGCCGGCCTCGACGCCGATCCCGGCGGCGCCGCGGCGGTTCGCGC
AGCCGTTCGCGCAGCCCGCCCGCGCGTTCGCCGCCGCGAGCGCCTGCGCGCCGTCCTGGCCGCGCTGGGACCGTTTCAAG
CGTGACTTCGTATCGGCCGACGGCCGCGTGATCGACGTCGGCTCGGCCGACGAGCGGACCGTATCCGAGGGGCAGGCGTA
CGGCCTTTTCTTCGCGCTCGTCGCGAACGACCGCGCGGCGTTCGACGCGCTGCTGCGCTGGACCGAGGACAATCTCGCGC
AGGGCGATCTGAGCGCGCGTCTGCCCGCGTGGCTGTGGGGCCGCGCGGCCGACGGCGCGTGGCGCGTGCTCGATGCGAAC
GCCGCGTCCGACGCCGATCTGTGGCTTGCGTACGCGCTGCTCGAAGCGGGGCGCTTGTGGCGCGAGCGCAGCTACACGGC
GCGCGGCGCGTTGCTCGCGAAGCGCGTGCTCGACGAGGAGACCGCGACGCTGCCGGGGCTCGGTCTCGTGCTGCTGCCGG
GCCCGACGGGTTTTCGGCCGGCGCGCGACGCGTGGCGGCTGAATCCGAGCTATTCGCCGCCGCAGGCGATTCGCGGAATC
GGCGCGCATGTGCCCGACGACGCGCGCTGGGCGCGGCTCGCGGCGGGCGTCGGCCGCGTGCTGACCGACAGCGCGCCGCG
CGGCTTCGCGCCGGACTGGGCGCTGTATCGCGCGGGCCGCGGCTTCGAGCCGGACGCCGAAACGCATGCGGCGAGCGCGT
ACAACGCGATTCGCGTCTATCTGTGGGCGGGCATGCTCGATGCGGGCGATCCGCTGGCGCGGCCGCTCGTCGCGCATTTC
GCGCCGTTCGCCGAGCATGTCGCCGCGCATGGCGCGCCGCCGGAGGCGGTCGATGCGACGACGGGCACGGCCGCCCCGCG
CGACGGCAATGCCGGGTTTTCCGCGGCGGCCGTGCCGTTTCTCGAGGCGCGCGGCGAGCGGGCGAGCGCCGACGCGCAGC
TCGCGCGCGTCGCGCGGCTCGAGCGCGAGACGGCGAGCGGCTATTACGCGAACGTGCTGACGCTGTTCGGGCTCGGCTGG
CGCGACGGGCGCTACCGGTTCGCGGCCGACGGCACGCTGCGGGTGCGATGGAGCGAGCCGTGCTCGACGCCCGCGCGTTG
A

Upstream 100 bases:

>100_bases
CTGCTCGGGCTGCTCGGGGCGGCGGCCGGGCTGCTGCTCGCGCTCGGCGCGTTCGGCGCGTTGCAGCGGATCGCCGCGCG
GCGGCGAGGGCTCTGACGCG

Downstream 100 bases:

>100_bases
CGGCGGGGCCGCAGACGAGGCGGCGCGCGCGGCGCGGCGCGGGCTGCGCCGGGATCGCGGCCGTCGCGGCGATCGCGTTC
GCCGTGCCATGCTTCGCGTC

Product: endo-1,4-D-glucanase

Products: NA

Alternate protein names: Carboxymethylcellulase; CMCase; Cellulase; Endo-1,4-beta-glucanase [H]

Number of amino acids: Translated: 506; Mature: 505

Protein sequence:

>506_residues
MARQAGRDEGVGRIGREAGDAREAAGAGEACGRRHARADAWARRASRASRGWRTFWRGRGGVRAVVASVVASFSVMAFAA
ATLPVSWRVAAAAERTRSGGERAGGLRDTAGLIEISAAAPASTPIPAAPRRFAQPFAQPARAFAAASACAPSWPRWDRFK
RDFVSADGRVIDVGSADERTVSEGQAYGLFFALVANDRAAFDALLRWTEDNLAQGDLSARLPAWLWGRAADGAWRVLDAN
AASDADLWLAYALLEAGRLWRERSYTARGALLAKRVLDEETATLPGLGLVLLPGPTGFRPARDAWRLNPSYSPPQAIRGI
GAHVPDDARWARLAAGVGRVLTDSAPRGFAPDWALYRAGRGFEPDAETHAASAYNAIRVYLWAGMLDAGDPLARPLVAHF
APFAEHVAAHGAPPEAVDATTGTAAPRDGNAGFSAAAVPFLEARGERASADAQLARVARLERETASGYYANVLTLFGLGW
RDGRYRFAADGTLRVRWSEPCSTPAR

Sequences:

>Translated_506_residues
MARQAGRDEGVGRIGREAGDAREAAGAGEACGRRHARADAWARRASRASRGWRTFWRGRGGVRAVVASVVASFSVMAFAA
ATLPVSWRVAAAAERTRSGGERAGGLRDTAGLIEISAAAPASTPIPAAPRRFAQPFAQPARAFAAASACAPSWPRWDRFK
RDFVSADGRVIDVGSADERTVSEGQAYGLFFALVANDRAAFDALLRWTEDNLAQGDLSARLPAWLWGRAADGAWRVLDAN
AASDADLWLAYALLEAGRLWRERSYTARGALLAKRVLDEETATLPGLGLVLLPGPTGFRPARDAWRLNPSYSPPQAIRGI
GAHVPDDARWARLAAGVGRVLTDSAPRGFAPDWALYRAGRGFEPDAETHAASAYNAIRVYLWAGMLDAGDPLARPLVAHF
APFAEHVAAHGAPPEAVDATTGTAAPRDGNAGFSAAAVPFLEARGERASADAQLARVARLERETASGYYANVLTLFGLGW
RDGRYRFAADGTLRVRWSEPCSTPAR
>Mature_505_residues
ARQAGRDEGVGRIGREAGDAREAAGAGEACGRRHARADAWARRASRASRGWRTFWRGRGGVRAVVASVVASFSVMAFAAA
TLPVSWRVAAAAERTRSGGERAGGLRDTAGLIEISAAAPASTPIPAAPRRFAQPFAQPARAFAAASACAPSWPRWDRFKR
DFVSADGRVIDVGSADERTVSEGQAYGLFFALVANDRAAFDALLRWTEDNLAQGDLSARLPAWLWGRAADGAWRVLDANA
ASDADLWLAYALLEAGRLWRERSYTARGALLAKRVLDEETATLPGLGLVLLPGPTGFRPARDAWRLNPSYSPPQAIRGIG
AHVPDDARWARLAAGVGRVLTDSAPRGFAPDWALYRAGRGFEPDAETHAASAYNAIRVYLWAGMLDAGDPLARPLVAHFA
PFAEHVAAHGAPPEAVDATTGTAAPRDGNAGFSAAAVPFLEARGERASADAQLARVARLERETASGYYANVLTLFGLGWR
DGRYRFAADGTLRVRWSEPCSTPAR

Specific function: Hydrolyzes carboxymethylcellulose [H]

COG id: COG3405

COG function: function code G; Endoglucanase Y

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 8 (cellulase D) family [H]

Homologues:

Organism=Escherichia coli, GI1789951, Length=365, Percent_Identity=47.3972602739726, Blast_Score=333, Evalue=2e-92,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008928
- InterPro:   IPR012341
- InterPro:   IPR002037
- InterPro:   IPR019834 [H]

Pfam domain/function: PF01270 Glyco_hydro_8 [H]

EC number: =3.2.1.4 [H]

Molecular weight: Translated: 53964; Mature: 53833

Theoretical pI: Translated: 10.31; Mature: 10.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARQAGRDEGVGRIGREAGDAREAAGAGEACGRRHARADAWARRASRASRGWRTFWRGRG
CCCCCCCCCCHHHHHHHCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
GVRAVVASVVASFSVMAFAAATLPVSWRVAAAAERTRSGGERAGGLRDTAGLIEISAAAP
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHCCCCCCCCCEEEEECCCC
ASTPIPAAPRRFAQPFAQPARAFAAASACAPSWPRWDRFKRDFVSADGRVIDVGSADERT
CCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCEEECCCCCCHH
VSEGQAYGLFFALVANDRAAFDALLRWTEDNLAQGDLSARLPAWLWGRAADGAWRVLDAN
HHCCCHHEEEEEEECCCHHHHHHHHHHCCCCCCCCCHHHCCCHHHCCCCCCCCEEEEECC
AASDADLWLAYALLEAGRLWRERSYTARGALLAKRVLDEETATLPGLGLVLLPGPTGFRP
CCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCC
ARDAWRLNPSYSPPQAIRGIGAHVPDDARWARLAAGVGRVLTDSAPRGFAPDWALYRAGR
CCHHEECCCCCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHCCC
GFEPDAETHAASAYNAIRVYLWAGMLDAGDPLARPLVAHFAPFAEHVAAHGAPPEAVDAT
CCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
TGTAAPRDGNAGFSAAAVPFLEARGERASADAQLARVARLERETASGYYANVLTLFGLGW
CCCCCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCC
RDGRYRFAADGTLRVRWSEPCSTPAR
CCCCEEEECCCEEEEEECCCCCCCCC
>Mature Secondary Structure 
ARQAGRDEGVGRIGREAGDAREAAGAGEACGRRHARADAWARRASRASRGWRTFWRGRG
CCCCCCCCCHHHHHHHCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
GVRAVVASVVASFSVMAFAAATLPVSWRVAAAAERTRSGGERAGGLRDTAGLIEISAAAP
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHCCCCCCCCCEEEEECCCC
ASTPIPAAPRRFAQPFAQPARAFAAASACAPSWPRWDRFKRDFVSADGRVIDVGSADERT
CCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCEEECCCCCCHH
VSEGQAYGLFFALVANDRAAFDALLRWTEDNLAQGDLSARLPAWLWGRAADGAWRVLDAN
HHCCCHHEEEEEEECCCHHHHHHHHHHCCCCCCCCCHHHCCCHHHCCCCCCCCEEEEECC
AASDADLWLAYALLEAGRLWRERSYTARGALLAKRVLDEETATLPGLGLVLLPGPTGFRP
CCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCC
ARDAWRLNPSYSPPQAIRGIGAHVPDDARWARLAAGVGRVLTDSAPRGFAPDWALYRAGR
CCHHEECCCCCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHCCC
GFEPDAETHAASAYNAIRVYLWAGMLDAGDPLARPLVAHFAPFAEHVAAHGAPPEAVDAT
CCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
TGTAAPRDGNAGFSAAAVPFLEARGERASADAQLARVARLERETASGYYANVLTLFGLGW
CCCCCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCC
RDGRYRFAADGTLRVRWSEPCSTPAR
CCCCEEEECCCEEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]