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The map label for this gene is hyfE [C]

Identifier: 126442939

GI number: 126442939

Start: 1544550

End: 1545209

Strand: Reverse

Name: hyfE [C]

Synonym: BURPS668_A1615

Alternate gene names: 126442939

Gene position: 1545209-1544550 (Counterclockwise)

Preceding gene: 126442527

Following gene: 126443859

Centisome position: 49.41

GC content: 64.39

Gene sequence:

>660_bases
ATGCACGGCTACGCGACGCAGCTCGTCAACTTCCTCGCGGCGATCCTGCTGCTGCTGTCGTTCGCGATGCTGAGCCAGCG
GCGGATCCTGTCGCTGATCCACCTGTATACGCTGCAGGGCGTCGCGCTCGTGTCGGCGAACCTGATTCTCGGCTTCGTGA
CGGCGGACACGCATCTGTATGTCTCCGCCGCGCTCACGCTGCTGCTGAAGGTCGGGCTGATTCCGTGGATCCTCTACCGG
CTCGTGCAGCGGCTGAACGTGAAGGCCGACGTCGAGCCGCTGCTGAACATTCCGACCACGCTCGTCGCCGGCATCGCGCT
CGTGATCGTCGCGTTCAACGTCGCGGCGCCGATCAGCCGGCTCGCCGAATCGGCCGCGCGCGGCACGCTCGGCATCGCGC
TCGCGTGCGTGCTGCTGTCGTTCATGATGATGATCACGCGCGCGAAGGCGATTCCGCAGGTGATCGGCTTCCTGTCGATG
GAAAACGGCCTGTTCTTCGCGGCCGCGGCGGCGACCAACGGGATGCCGATGATCGTCGAGCTCGGCATCGGGCTCGACGT
GCTGGTCGGCATCCTGATCCTCGGCGTGTTCATGTTCCAGATCCGCGAGCAGTTCGACAGCCTCGACATCCACCACCTCG
AGAAACTGAAAGATGATTGA

Upstream 100 bases:

>100_bases
GAACGCGAAAATGCGCCTCTTTCGCGTGCCCGAATTCCTCGCGAGCGCGTTCCTGCTCGCGGTGATCGGCATGCTCGTCC
ATTTCCTGCTGGGGGCGTAG

Downstream 100 bases:

>100_bases
CGGCTACGTGCTGCCCGCGTTGCTCGGCCTGCCGCTCGCGGCGGGCGGGTGCCTCGCGCTCGTGCGGCGCCAGCACGTCG
CGCGCGCGCTGAACGTCGGC

Product: putative hydrogenase, membrane subunit

Products: H2; oxidized ferredoxin [C]

Alternate protein names: NA

Number of amino acids: Translated: 219; Mature: 219

Protein sequence:

>219_residues
MHGYATQLVNFLAAILLLLSFAMLSQRRILSLIHLYTLQGVALVSANLILGFVTADTHLYVSAALTLLLKVGLIPWILYR
LVQRLNVKADVEPLLNIPTTLVAGIALVIVAFNVAAPISRLAESAARGTLGIALACVLLSFMMMITRAKAIPQVIGFLSM
ENGLFFAAAAATNGMPMIVELGIGLDVLVGILILGVFMFQIREQFDSLDIHHLEKLKDD

Sequences:

>Translated_219_residues
MHGYATQLVNFLAAILLLLSFAMLSQRRILSLIHLYTLQGVALVSANLILGFVTADTHLYVSAALTLLLKVGLIPWILYR
LVQRLNVKADVEPLLNIPTTLVAGIALVIVAFNVAAPISRLAESAARGTLGIALACVLLSFMMMITRAKAIPQVIGFLSM
ENGLFFAAAAATNGMPMIVELGIGLDVLVGILILGVFMFQIREQFDSLDIHHLEKLKDD
>Mature_219_residues
MHGYATQLVNFLAAILLLLSFAMLSQRRILSLIHLYTLQGVALVSANLILGFVTADTHLYVSAALTLLLKVGLIPWILYR
LVQRLNVKADVEPLLNIPTTLVAGIALVIVAFNVAAPISRLAESAARGTLGIALACVLLSFMMMITRAKAIPQVIGFLSM
ENGLFFAAAAATNGMPMIVELGIGLDVLVGILILGVFMFQIREQFDSLDIHHLEKLKDD

Specific function: Unknown

COG id: COG4237

COG function: function code C; Hydrogenase 4 membrane component (E)

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 1.-.-.- [C]

Molecular weight: Translated: 23694; Mature: 23694

Theoretical pI: Translated: 7.73; Mature: 7.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHGYATQLVNFLAAILLLLSFAMLSQRRILSLIHLYTLQGVALVSANLILGFVTADTHLY
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VSAALTLLLKVGLIPWILYRLVQRLNVKADVEPLLNIPTTLVAGIALVIVAFNVAAPISR
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
LAESAARGTLGIALACVLLSFMMMITRAKAIPQVIGFLSMENGLFFAAAAATNGMPMIVE
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEHHHCCCCCEEEE
LGIGLDVLVGILILGVFMFQIREQFDSLDIHHLEKLKDD
ECCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCC
>Mature Secondary Structure
MHGYATQLVNFLAAILLLLSFAMLSQRRILSLIHLYTLQGVALVSANLILGFVTADTHLY
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VSAALTLLLKVGLIPWILYRLVQRLNVKADVEPLLNIPTTLVAGIALVIVAFNVAAPISR
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
LAESAARGTLGIALACVLLSFMMMITRAKAIPQVIGFLSMENGLFFAAAAATNGMPMIVE
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEHHHCCCCCEEEE
LGIGLDVLVGILILGVFMFQIREQFDSLDIHHLEKLKDD
ECCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton; reduced ferredoxin [C]

Specific reaction: Proton + reduced ferredoxin = H2 + oxidized ferredoxin [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]