Click here to switch to the map view.

The map label for this gene is pksH [H]

Identifier: 126442412

GI number: 126442412

Start: 1368438

End: 1369208

Strand: Reverse

Name: pksH [H]

Synonym: BURPS668_A1469

Alternate gene names: 126442412

Gene position: 1369208-1368438 (Counterclockwise)

Preceding gene: 126443435

Following gene: 126444812

Centisome position: 43.78

GC content: 66.41

Gene sequence:

>771_bases
ATGCTGAGCCGGCCCGCCGACTACCGGACCATCCGCGTCCGGTACGACGGCGACATCTGCTTCCTGCAGCTTCATCGCCC
CGACGCGCAGAACGCGATCAACAACCGCATGATCGCGGAGTGCATGGACGTGCTCGATCGCTGCGAGCACGCGGCGAAGA
TCGTCGTGTTGCAAGGGCTGCCCGACGTGTTCTGCTTCGGCGCGGACTTCTCCGACATTGCCGAAAAGCCGGATGCGCTG
ATCGATTCCGATGCGATCTACGGCTTATGGCACCGGCTCGCGACGGGGCCGTACATCACGATCGCGCACGTGCAGGGCAA
GGTCAACGCGGGCGGCATCGGCTTCGTCGCCGCGTCCGACATCGCGATCTGCGACGAGAACGTCCCGTTCAGCCTGTCGG
AGCTGCTGTTCGGCCTGATCCCGGCGTGCGTGCTGCCGTTCCTCGCGCGCCGCATCGGCACGCCGAAAGCGCATTACATG
ACGCTGATGACGCAGCCCGTCACCGCGCAGCAGGCCTTCTCCTGGGGGCTCGTCGACGCGATCGGCGCCAATACGGACGC
GCTCGTGCGCAAGCATCTGCTGCGCCTGCGATGCCTGAACAAGGCGTCCGTCGCGCGATACAAGTCGTACGCGGCGACGC
TCGACGACACGCTCGCCGCGGCGCGCCCGGGCGCCGTGCGCGTGAGCATCGAGATGTTCGCCGATCCGGAGAACCGCCGG
AAGATCGCGCGGTACGTCGACACCGGAAAATTTCCCTGGGAGGCGGACTAA

Upstream 100 bases:

>100_bases
AAATCGACGACGGCTTCATCCCCGCGGCGCGGCGCGCGCACGGCCGCGAAACGCTCTTCCTGAGCCGGATCAACGAATAC
CATCGGGAATACGAATGGAT

Downstream 100 bases:

>100_bases
GTGAGCGCGCATGAACGAGTCGTCGAAGTCCGCGAGGTGGCGGACTGCGTAGTCCAGGTCGTCATGCAGGATCGCGAGAA
CAAGAACACGTTCTCCGACG

Product: polyketide biosynthesis enoyl-CoA hydratase

Products: trans-2-enoyl-CoA; trans-3-enoyl-CoA; H2O

Alternate protein names: NA

Number of amino acids: Translated: 256; Mature: 256

Protein sequence:

>256_residues
MLSRPADYRTIRVRYDGDICFLQLHRPDAQNAINNRMIAECMDVLDRCEHAAKIVVLQGLPDVFCFGADFSDIAEKPDAL
IDSDAIYGLWHRLATGPYITIAHVQGKVNAGGIGFVAASDIAICDENVPFSLSELLFGLIPACVLPFLARRIGTPKAHYM
TLMTQPVTAQQAFSWGLVDAIGANTDALVRKHLLRLRCLNKASVARYKSYAATLDDTLAAARPGAVRVSIEMFADPENRR
KIARYVDTGKFPWEAD

Sequences:

>Translated_256_residues
MLSRPADYRTIRVRYDGDICFLQLHRPDAQNAINNRMIAECMDVLDRCEHAAKIVVLQGLPDVFCFGADFSDIAEKPDAL
IDSDAIYGLWHRLATGPYITIAHVQGKVNAGGIGFVAASDIAICDENVPFSLSELLFGLIPACVLPFLARRIGTPKAHYM
TLMTQPVTAQQAFSWGLVDAIGANTDALVRKHLLRLRCLNKASVARYKSYAATLDDTLAAARPGAVRVSIEMFADPENRR
KIARYVDTGKFPWEAD
>Mature_256_residues
MLSRPADYRTIRVRYDGDICFLQLHRPDAQNAINNRMIAECMDVLDRCEHAAKIVVLQGLPDVFCFGADFSDIAEKPDAL
IDSDAIYGLWHRLATGPYITIAHVQGKVNAGGIGFVAASDIAICDENVPFSLSELLFGLIPACVLPFLARRIGTPKAHYM
TLMTQPVTAQQAFSWGLVDAIGANTDALVRKHLLRLRCLNKASVARYKSYAATLDDTLAAARPGAVRVSIEMFADPENRR
KIARYVDTGKFPWEAD

Specific function: Involved in some intermediate steps for the synthesis of the antibiotic polyketide bacillaene which is involved in secondary metabolism. Probably catalyzes the dehydration of the (S)-3-hydroxy-3-methylglutaryl group attached to pksL [H]

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Escherichia coli, GI1787659, Length=167, Percent_Identity=33.5329341317365, Blast_Score=79, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: 4.2.1.17

Molecular weight: Translated: 28241; Mature: 28241

Theoretical pI: Translated: 7.07; Mature: 7.07

Prosite motif: PS00213 LIPOCALIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSRPADYRTIRVRYDGDICFLQLHRPDAQNAINNRMIAECMDVLDRCEHAAKIVVLQGL
CCCCCCCCEEEEEEECCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCC
PDVFCFGADFSDIAEKPDALIDSDAIYGLWHRLATGPYITIAHVQGKVNAGGIGFVAASD
CCEEEECCCHHHHHCCCCHHHCCHHHHHHHHHHCCCCEEEEEEEECCCCCCCEEEEEECC
IAICDENVPFSLSELLFGLIPACVLPFLARRIGTPKAHYMTLMTQPVTAQQAFSWGLVDA
EEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHEEEEEECCCCHHHHHCCCCHHH
IGANTDALVRKHLLRLRCLNKASVARYKSYAATLDDTLAAARPGAVRVSIEMFADPENRR
HCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCHHHH
KIARYVDTGKFPWEAD
HHHHHHHCCCCCCCCC
>Mature Secondary Structure
MLSRPADYRTIRVRYDGDICFLQLHRPDAQNAINNRMIAECMDVLDRCEHAAKIVVLQGL
CCCCCCCCEEEEEEECCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCC
PDVFCFGADFSDIAEKPDALIDSDAIYGLWHRLATGPYITIAHVQGKVNAGGIGFVAASD
CCEEEECCCHHHHHCCCCHHHCCHHHHHHHHHHCCCCEEEEEEEECCCCCCCEEEEEECC
IAICDENVPFSLSELLFGLIPACVLPFLARRIGTPKAHYMTLMTQPVTAQQAFSWGLVDA
EEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHEEEEEECCCCHHHHHCCCCHHH
IGANTDALVRKHLLRLRCLNKASVARYKSYAATLDDTLAAARPGAVRVSIEMFADPENRR
HCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCHHHH
KIARYVDTGKFPWEAD
HHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.05 {crotonyl-CoA}} 0.008 {2-decenoyl-CoA}} [C]

Substrates: (3S)-3-hydroxyacyl-CoA

Specific reaction: (3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H2O

General reaction: addition (of H2O to carbon carbon double bondelimination (of H2O C-O bond cleavagehydration [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7704258; 9384377 [H]