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The map label for this gene is lgrC [H]

Identifier: 126442359

GI number: 126442359

Start: 2500266

End: 2501855

Strand: Reverse

Name: lgrC [H]

Synonym: BURPS668_A2598

Alternate gene names: 126442359

Gene position: 2501855-2500266 (Counterclockwise)

Preceding gene: 126444621

Following gene: 126445578

Centisome position: 80.0

GC content: 65.16

Gene sequence:

>1590_bases
ATGGCCAGACACATCGTTCAAGCAATCCAGTCTCACGCGTTCGCGACGCCGTACAAATGCGCGTTGTCCGATTCCCGCGG
CGATCTCGGCTACGCCGATCTCGACAGCTTCAGCACGCGTTTTGCGATGCGCCTGCAGGATCTGGGCTGTCGCCCCGGCG
ATCGCGTCGTGATGCTCGCGAGCCGCCGCGCGCTGCTTGTCGCGGCGATCATCGGCGTGTTCAAGGCAGGCTGCGTGCAT
GTGCCGCTCGATCCGCGCATGCCGGCCGACCGTCTTCGCTACATCCTGCACGACGTCGCGCCGACGCTCGTGATCGCCGA
CGAGGATCTGATCGACGCGATCGAACACGCGCTGCCGGCCGCCGCGCCGATCGTGCCCGTGACCGAGCTCGAGCGGCTTC
TAGACGACGCCGATTCGCCGCGGCTCGACGCGCTCGTGCAGCCGTTACCGCTGCCGCCGCTCGACGAGCGAGCGATCGCG
TACTGCATCTACACGTCGGGCTCGACCGGCCGCCCGAAAGGCGTGCTGATCAATCATCGCAGCATCGCAGATTTCTTCGA
GGGCACGCACGCCGTCTACGATGTCACGGCGCAATCGCGCTGCGCGAGCTTCTCGCCGCTGAACTTCGACGTGTACCTGA
TGGACATGCTGTTTCCGCTGGCGCAGGGCGCGTCGCTATACGTGCACGACGACGTGAACGCGCCCGATCTGCTGTTCGAC
GCGATTCGCGCGCACGACGTCACCCACTTTTCCGCGTGGGGAATGATGCTCGGCCTGATTGCGCAAGCTGAGGAATTCGA
ATCCGCGCCGCTGCCGCATCTGAAGACGATCCTCACCGGCACCGACGTGCCCGACGTGAAGACGGTCCAGCGCTGGCTCA
GGAAGAACGCGGGCGTGCAGGTGATCAACGCCTACGGGCCGACCGAAGCCACCTGCGCGGCGACCGCGCACGTGATCCGC
GAGATCGAGCCGGAGCGGCGCACGCTCTACCCGATCGGCAAGCCGCTCGAGCACGTGCTGGCGCTGCTCGTCGACGAGGG
CGGCAACCGAATCACGGCGCCGGGCGTGCCGGGCGAGTTGATGATCGGCGGCACGCAGGTAATGCAGGGCTACTGGAATC
TGCCGGAAGAAACGGCGGCGCGGCTCGTGCGTCTCGACGGCGTGCCGTTCTATCGAACGGGCGACGTCTGCGCGTATCTC
GCCGACGGCAGCCTCTACTACATGGGCCGCAAGGATAACGAAGTGAAGATCGGCGGCTACCGGATCCATTTGAGCGAAAT
CCAGCGGGTCATCAACAGCGTGCCGCACGTGTACGGATCGGAGGTGGTGCTGCTCGAATCGCGCTACGGCGAGACGCTGC
TCGCCGCCGGCGTGCTGCTCGAACGCGGCGCGCCGCTCGACGCCGATTGCAAGGCCGACGAAATCAGGCAGCGCCTCTCG
GCGGAGCTGCCCGCCTACATGGTGCCCCGCCACGTCAAGGTTCTCGAGCAGTTTCCGCAGTTGTCATCGGGAAAGACGGA
TCGCAAAGCGCTTCTGTCGATATTGCAACAGCGCATCAACGAAAGTAACCAGGAGGAAGTGAATCCATGA

Upstream 100 bases:

>100_bases
CGAATGCGCATGCCGTGCCGTCCGGCCGTCTGCCGGTGCGCAATGCCGCCTGAATCTTGAAGGTGCCCGTAAAGAGACAT
CGCAAAGAGGATTCAGTCAG

Downstream 100 bases:

>100_bases
AAACCGAAACGATGTTGATTCAGATTCTGGAGAGCGTGATCGGCGTCAAGAAGGTGACCGGCGATACCCGTTTTCTGGAC
ATCGGCGGCAATTCGCTCAA

Product: amino acid adenylation domain-containing protein

Products: pyrophosphate; AMP; enterobactin; pyrophosphate; L-Seryl-AMP [C]

Alternate protein names: ATP-dependent valine adenylase; ValA; Valine activase; ATP-dependent D-valine adenylase; D-ValA; D-valine activase; Valine racemase [ATP-hydrolyzing]; ATP-dependent tryptophan adenylase; TrpA; Tryptophan activase; ATP-dependent D-leucine adenylase; D-LeuA; D-leucine activase; Leucine racemase [ATP-hydrolyzing]; ATP-dependent tryptophan/phenylalanine/tyrosine adenylase; Trp/Phe/TyrA; Tryptophan/phenylalanine/tyrosine activase; ATP-dependent D-leucine adenylase; D-LeuA; D-leucine activase; Leucine racemase [ATP-hydrolyzing] [H]

Number of amino acids: Translated: 529; Mature: 528

Protein sequence:

>529_residues
MARHIVQAIQSHAFATPYKCALSDSRGDLGYADLDSFSTRFAMRLQDLGCRPGDRVVMLASRRALLVAAIIGVFKAGCVH
VPLDPRMPADRLRYILHDVAPTLVIADEDLIDAIEHALPAAAPIVPVTELERLLDDADSPRLDALVQPLPLPPLDERAIA
YCIYTSGSTGRPKGVLINHRSIADFFEGTHAVYDVTAQSRCASFSPLNFDVYLMDMLFPLAQGASLYVHDDVNAPDLLFD
AIRAHDVTHFSAWGMMLGLIAQAEEFESAPLPHLKTILTGTDVPDVKTVQRWLRKNAGVQVINAYGPTEATCAATAHVIR
EIEPERRTLYPIGKPLEHVLALLVDEGGNRITAPGVPGELMIGGTQVMQGYWNLPEETAARLVRLDGVPFYRTGDVCAYL
ADGSLYYMGRKDNEVKIGGYRIHLSEIQRVINSVPHVYGSEVVLLESRYGETLLAAGVLLERGAPLDADCKADEIRQRLS
AELPAYMVPRHVKVLEQFPQLSSGKTDRKALLSILQQRINESNQEEVNP

Sequences:

>Translated_529_residues
MARHIVQAIQSHAFATPYKCALSDSRGDLGYADLDSFSTRFAMRLQDLGCRPGDRVVMLASRRALLVAAIIGVFKAGCVH
VPLDPRMPADRLRYILHDVAPTLVIADEDLIDAIEHALPAAAPIVPVTELERLLDDADSPRLDALVQPLPLPPLDERAIA
YCIYTSGSTGRPKGVLINHRSIADFFEGTHAVYDVTAQSRCASFSPLNFDVYLMDMLFPLAQGASLYVHDDVNAPDLLFD
AIRAHDVTHFSAWGMMLGLIAQAEEFESAPLPHLKTILTGTDVPDVKTVQRWLRKNAGVQVINAYGPTEATCAATAHVIR
EIEPERRTLYPIGKPLEHVLALLVDEGGNRITAPGVPGELMIGGTQVMQGYWNLPEETAARLVRLDGVPFYRTGDVCAYL
ADGSLYYMGRKDNEVKIGGYRIHLSEIQRVINSVPHVYGSEVVLLESRYGETLLAAGVLLERGAPLDADCKADEIRQRLS
AELPAYMVPRHVKVLEQFPQLSSGKTDRKALLSILQQRINESNQEEVNP
>Mature_528_residues
ARHIVQAIQSHAFATPYKCALSDSRGDLGYADLDSFSTRFAMRLQDLGCRPGDRVVMLASRRALLVAAIIGVFKAGCVHV
PLDPRMPADRLRYILHDVAPTLVIADEDLIDAIEHALPAAAPIVPVTELERLLDDADSPRLDALVQPLPLPPLDERAIAY
CIYTSGSTGRPKGVLINHRSIADFFEGTHAVYDVTAQSRCASFSPLNFDVYLMDMLFPLAQGASLYVHDDVNAPDLLFDA
IRAHDVTHFSAWGMMLGLIAQAEEFESAPLPHLKTILTGTDVPDVKTVQRWLRKNAGVQVINAYGPTEATCAATAHVIRE
IEPERRTLYPIGKPLEHVLALLVDEGGNRITAPGVPGELMIGGTQVMQGYWNLPEETAARLVRLDGVPFYRTGDVCAYLA
DGSLYYMGRKDNEVKIGGYRIHLSEIQRVINSVPHVYGSEVVLLESRYGETLLAAGVLLERGAPLDADCKADEIRQRLSA
ELPAYMVPRHVKVLEQFPQLSSGKTDRKALLSILQQRINESNQEEVNP

Specific function: Activates the 7th to 12th amino acids (Val, D-Val, Trp, D-Leu, Xaa and D-Leu) in linear gramicidin and catalyzes the formation of the peptide bond between them. This enzyme is also responsible for the epimerization of the 8th (D-Val), the 10th (D- Leu) an

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 6 acyl carrier domains [H]

Homologues:

Organism=Homo sapiens, GI187761345, Length=547, Percent_Identity=25.2285191956124, Blast_Score=125, Evalue=7e-29,
Organism=Homo sapiens, GI187761343, Length=547, Percent_Identity=25.2285191956124, Blast_Score=125, Evalue=7e-29,
Organism=Homo sapiens, GI42544132, Length=501, Percent_Identity=23.75249500998, Blast_Score=96, Evalue=1e-19,
Organism=Homo sapiens, GI156151445, Length=558, Percent_Identity=22.0430107526882, Blast_Score=93, Evalue=5e-19,
Organism=Homo sapiens, GI45580730, Length=384, Percent_Identity=25.5208333333333, Blast_Score=92, Evalue=1e-18,
Organism=Homo sapiens, GI115511026, Length=485, Percent_Identity=22.8865979381443, Blast_Score=91, Evalue=3e-18,
Organism=Homo sapiens, GI58082049, Length=482, Percent_Identity=22.1991701244813, Blast_Score=91, Evalue=3e-18,
Organism=Homo sapiens, GI157311624, Length=487, Percent_Identity=22.5872689938398, Blast_Score=89, Evalue=1e-17,
Organism=Homo sapiens, GI157311622, Length=487, Percent_Identity=22.5872689938398, Blast_Score=89, Evalue=1e-17,
Organism=Homo sapiens, GI42794756, Length=537, Percent_Identity=24.3947858472998, Blast_Score=86, Evalue=8e-17,
Organism=Homo sapiens, GI42794760, Length=537, Percent_Identity=24.3947858472998, Blast_Score=86, Evalue=1e-16,
Organism=Homo sapiens, GI42794758, Length=537, Percent_Identity=24.3947858472998, Blast_Score=86, Evalue=1e-16,
Organism=Homo sapiens, GI38505220, Length=487, Percent_Identity=24.2299794661191, Blast_Score=83, Evalue=5e-16,
Organism=Homo sapiens, GI122937307, Length=515, Percent_Identity=22.1359223300971, Blast_Score=78, Evalue=3e-14,
Organism=Escherichia coli, GI1786801, Length=527, Percent_Identity=29.7912713472486, Blast_Score=186, Evalue=3e-48,
Organism=Escherichia coli, GI145693145, Length=538, Percent_Identity=26.3940520446097, Blast_Score=109, Evalue=3e-25,
Organism=Escherichia coli, GI1788107, Length=521, Percent_Identity=26.295585412668, Blast_Score=109, Evalue=6e-25,
Organism=Escherichia coli, GI1786810, Length=514, Percent_Identity=23.5408560311284, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1789201, Length=383, Percent_Identity=24.5430809399478, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI1786529, Length=525, Percent_Identity=21.3333333333333, Blast_Score=65, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17558820, Length=491, Percent_Identity=22.6069246435845, Blast_Score=101, Evalue=9e-22,
Organism=Caenorhabditis elegans, GI71994703, Length=533, Percent_Identity=21.7636022514071, Blast_Score=93, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI71994694, Length=533, Percent_Identity=21.7636022514071, Blast_Score=93, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI71994690, Length=533, Percent_Identity=21.7636022514071, Blast_Score=92, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI17556356, Length=368, Percent_Identity=23.6413043478261, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17560308, Length=520, Percent_Identity=20.7692307692308, Blast_Score=81, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17559526, Length=385, Percent_Identity=23.1168831168831, Blast_Score=77, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI115534440, Length=521, Percent_Identity=22.8406909788868, Blast_Score=74, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI71985884, Length=395, Percent_Identity=22.0253164556962, Blast_Score=69, Evalue=5e-12,
Organism=Saccharomyces cerevisiae, GI6319591, Length=572, Percent_Identity=25.6993006993007, Blast_Score=130, Evalue=5e-31,
Organism=Saccharomyces cerevisiae, GI6319699, Length=371, Percent_Identity=24.5283018867925, Blast_Score=94, Evalue=7e-20,
Organism=Drosophila melanogaster, GI24648676, Length=485, Percent_Identity=28.4536082474227, Blast_Score=135, Evalue=6e-32,
Organism=Drosophila melanogaster, GI19922652, Length=514, Percent_Identity=24.9027237354086, Blast_Score=102, Evalue=8e-22,
Organism=Drosophila melanogaster, GI24648260, Length=505, Percent_Identity=22.5742574257426, Blast_Score=94, Evalue=3e-19,
Organism=Drosophila melanogaster, GI24581924, Length=517, Percent_Identity=24.1779497098646, Blast_Score=93, Evalue=5e-19,
Organism=Drosophila melanogaster, GI24582852, Length=468, Percent_Identity=24.3589743589744, Blast_Score=87, Evalue=4e-17,
Organism=Drosophila melanogaster, GI281365686, Length=385, Percent_Identity=24.4155844155844, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI21356947, Length=503, Percent_Identity=22.4652087475149, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI21356441, Length=489, Percent_Identity=21.4723926380368, Blast_Score=84, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24656500, Length=498, Percent_Identity=24.0963855421687, Blast_Score=84, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24653035, Length=513, Percent_Identity=23.7816764132554, Blast_Score=82, Evalue=6e-16,
Organism=Drosophila melanogaster, GI21358303, Length=490, Percent_Identity=22.0408163265306, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24648253, Length=485, Percent_Identity=22.680412371134, Blast_Score=80, Evalue=5e-15,
Organism=Drosophila melanogaster, GI24648255, Length=498, Percent_Identity=23.2931726907631, Blast_Score=79, Evalue=6e-15,
Organism=Drosophila melanogaster, GI161076582, Length=501, Percent_Identity=23.75249500998, Blast_Score=79, Evalue=1e-14,
Organism=Drosophila melanogaster, GI18859661, Length=379, Percent_Identity=26.1213720316623, Blast_Score=78, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010071
- InterPro:   IPR009081
- InterPro:   IPR020845
- InterPro:   IPR000873
- InterPro:   IPR023213
- InterPro:   IPR001242
- InterPro:   IPR010060
- InterPro:   IPR006163
- InterPro:   IPR020806
- InterPro:   IPR006162 [H]

Pfam domain/function: PF00501 AMP-binding; PF00668 Condensation; PF00550 PP-binding [H]

EC number: 2.7.7.- [C]

Molecular weight: Translated: 58181; Mature: 58050

Theoretical pI: Translated: 5.45; Mature: 5.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARHIVQAIQSHAFATPYKCALSDSRGDLGYADLDSFSTRFAMRLQDLGCRPGDRVVMLA
CHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEE
SRRALLVAAIIGVFKAGCVHVPLDPRMPADRLRYILHDVAPTLVIADEDLIDAIEHALPA
CCHHHHHHHHHHHHHCCEEECCCCCCCCHHHHHHHHHHHCCEEEEECHHHHHHHHHHCCC
AAPIVPVTELERLLDDADSPRLDALVQPLPLPPLDERAIAYCIYTSGSTGRPKGVLINHR
CCCCCCHHHHHHHHCCCCCCCHHHHHCCCCCCCCCCCEEEEEEEECCCCCCCCCEEEECC
SIADFFEGTHAVYDVTAQSRCASFSPLNFDVYLMDMLFPLAQGASLYVHDDVNAPDLLFD
HHHHHHCCCCCEEEECHHHHCCCCCCCCCHHHHHHHHHHHHCCCEEEEECCCCCHHHHHH
AIRAHDVTHFSAWGMMLGLIAQAEEFESAPLPHLKTILTGTDVPDVKTVQRWLRKNAGVQ
HHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCHHHHHHHHHHCCCEE
VINAYGPTEATCAATAHVIREIEPERRTLYPIGKPLEHVLALLVDEGGNRITAPGVPGEL
EEECCCCCCHHHHHHHHHHHHCCCCCCEECCCCCHHHHHHHHHHHCCCCEEECCCCCCCE
MIGGTQVMQGYWNLPEETAARLVRLDGVPFYRTGDVCAYLADGSLYYMGRKDNEVKIGGY
EECHHHHHHHHCCCCHHHHHHHHHCCCCCEEECCCEEEEEECCEEEEECCCCCEEEEEEE
RIHLSEIQRVINSVPHVYGSEVVLLESRYGETLLAAGVLLERGAPLDADCKADEIRQRLS
EEEHHHHHHHHHHCCHHCCCCEEEEECCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHH
AELPAYMVPRHVKVLEQFPQLSSGKTDRKALLSILQQRINESNQEEVNP
HCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
ARHIVQAIQSHAFATPYKCALSDSRGDLGYADLDSFSTRFAMRLQDLGCRPGDRVVMLA
HHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEE
SRRALLVAAIIGVFKAGCVHVPLDPRMPADRLRYILHDVAPTLVIADEDLIDAIEHALPA
CCHHHHHHHHHHHHHCCEEECCCCCCCCHHHHHHHHHHHCCEEEEECHHHHHHHHHHCCC
AAPIVPVTELERLLDDADSPRLDALVQPLPLPPLDERAIAYCIYTSGSTGRPKGVLINHR
CCCCCCHHHHHHHHCCCCCCCHHHHHCCCCCCCCCCCEEEEEEEECCCCCCCCCEEEECC
SIADFFEGTHAVYDVTAQSRCASFSPLNFDVYLMDMLFPLAQGASLYVHDDVNAPDLLFD
HHHHHHCCCCCEEEECHHHHCCCCCCCCCHHHHHHHHHHHHCCCEEEEECCCCCHHHHHH
AIRAHDVTHFSAWGMMLGLIAQAEEFESAPLPHLKTILTGTDVPDVKTVQRWLRKNAGVQ
HHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCHHHHHHHHHHCCCEE
VINAYGPTEATCAATAHVIREIEPERRTLYPIGKPLEHVLALLVDEGGNRITAPGVPGEL
EEECCCCCCHHHHHHHHHHHHCCCCCCEECCCCCHHHHHHHHHHHCCCCEEECCCCCCCE
MIGGTQVMQGYWNLPEETAARLVRLDGVPFYRTGDVCAYLADGSLYYMGRKDNEVKIGGY
EECHHHHHHHHCCCCHHHHHHHHHCCCCCEEECCCEEEEEECCEEEEECCCCCEEEEEEE
RIHLSEIQRVINSVPHVYGSEVVLLESRYGETLLAAGVLLERGAPLDADCKADEIRQRLS
EEEHHHHHHHHHHCCHHCCCCEEEEECCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHH
AELPAYMVPRHVKVLEQFPQLSSGKTDRKALLSILQQRINESNQEEVNP
HCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Phosphopantetheine. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 6 ATP; L-serine; 2,3-dihydroxybenzoate [C]

Specific reaction: 6 ATP + 3 L-serine + 3 2,3-dihydroxybenzoate = 6 pyrophosphate + 6 AMP + enterobactin ATP + L-serine = pyrophosphate + L-Seryl-AMP 6 ATP + 3 L-serine + 3 2,3-dihydroxybenzoate = 6 pyrophosphate + 6 AMP + enterobactin ATP + L-serine = pyrophosphate + L-Ser

General reaction: Transferases; Acyltransferases; Transferring groups other than amino-acyl groups [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA