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The map label for this gene is prfA [H]

Identifier: 126209498

GI number: 126209498

Start: 2270835

End: 2271836

Strand: Reverse

Name: prfA [H]

Synonym: APL_2044

Alternate gene names: 126209498

Gene position: 2271836-2270835 (Counterclockwise)

Preceding gene: 126209499

Following gene: 126209497

Centisome position: 99.88

GC content: 45.51

Gene sequence:

>1002_bases
GTGATTAATGATCAGGATAAATTCCGTGCTTATTCAAAAGAATATTCACAACTTGAAGAAGTTGTGACTACTTTCAATCG
TTGGAAAAAATTAAATAACGATATCGAAGAAGCACAAATTCTGCTTGATGATCCGGATATGAAAGATATGGCAGCGGAAG
AAATTGCGGAAAATAAAGCCGAAATCGAAAATCTGGAACAACACTTACAAATCTTATTACTGCCGAAAGATCCGAATGAC
GAATACAATGCATTCTTAGAGATTCGTGCCGGTACGGGCGGCGACGAAGCGGGGATTTTCGCCGGCGATTTATATCGTAT
GTACAGCCGTTATTGCGAAAGCAAACGCTGGAGAATCGAAGAAATGTCCGCTAACGAAAGCGAACAAGGCGGATATAAAG
AAATCATCGTTAAAATCAGCGGCGAAGGCGTGTACGGTCAGCTTAAATTCGAATCGGGCGGTCACCGTGTACAACGTGTG
CCGAAAACTGAATCACAAGGTCGTATTCACACCTCTGCGTGTACGGTTGCAGTAATGCCGGAATTACCGGAATCGGAAAT
GCCGGAAATCAACCCGACGGATTTACGTATCGATACTTATCGCTCATCAGGTGCCGGCGGTCAGCACGTTAATACCACCG
ATTCTGCGGTACGTATTACCCATATTCCGACCGGGATTGTAGTAGAGTGCCAAGACGAACGTTCACAGCACAAAAACAAA
GCGAAAGCACTTGCTGTGCTAGCTTCTCGTATTGTTCAGGTTGAGCAAGAACGCCAAGCGGCGGAACAAGCGGATACTCG
TCGTAACTTACTGGGTTCGGGCGACCGTTCGGATAAGATTCGTACCTATAACTATCCGCAAGGTCGTGTCACCGATCACC
GTATCAATCTAACCGTTTACCGCTTAGATGAAGTGATGAACGGTAAAATTGACGAGTTAATTCAGCCGATTATCACGGAA
TACCAAGCGGATCAGTTAGCCGCACTTTCCGAGCAGGCTTAA

Upstream 100 bases:

>100_bases
AACAGATATAAATCGAACAATGAAAGACTCTATTATTAATAAATTAGAAAGCCTGAGCGAACGTCATGAGGAATTGCAAG
CCTTATTAGGTGATCCGTCC

Downstream 100 bases:

>100_bases
GCGTGATGAGGGCGCAACCGCCCTCGTCTTTTTACTTAAATTACTTTTATTTTGGAAAGCATATGAAAAAAATTATTTTA
GCGGCAACAGTCAGCTTACT

Product: peptide chain release factor 1

Products: NA

Alternate protein names: RF-1 [H]

Number of amino acids: Translated: 333; Mature: 333

Protein sequence:

>333_residues
MINDQDKFRAYSKEYSQLEEVVTTFNRWKKLNNDIEEAQILLDDPDMKDMAAEEIAENKAEIENLEQHLQILLLPKDPND
EYNAFLEIRAGTGGDEAGIFAGDLYRMYSRYCESKRWRIEEMSANESEQGGYKEIIVKISGEGVYGQLKFESGGHRVQRV
PKTESQGRIHTSACTVAVMPELPESEMPEINPTDLRIDTYRSSGAGGQHVNTTDSAVRITHIPTGIVVECQDERSQHKNK
AKALAVLASRIVQVEQERQAAEQADTRRNLLGSGDRSDKIRTYNYPQGRVTDHRINLTVYRLDEVMNGKIDELIQPIITE
YQADQLAALSEQA

Sequences:

>Translated_333_residues
MINDQDKFRAYSKEYSQLEEVVTTFNRWKKLNNDIEEAQILLDDPDMKDMAAEEIAENKAEIENLEQHLQILLLPKDPND
EYNAFLEIRAGTGGDEAGIFAGDLYRMYSRYCESKRWRIEEMSANESEQGGYKEIIVKISGEGVYGQLKFESGGHRVQRV
PKTESQGRIHTSACTVAVMPELPESEMPEINPTDLRIDTYRSSGAGGQHVNTTDSAVRITHIPTGIVVECQDERSQHKNK
AKALAVLASRIVQVEQERQAAEQADTRRNLLGSGDRSDKIRTYNYPQGRVTDHRINLTVYRLDEVMNGKIDELIQPIITE
YQADQLAALSEQA
>Mature_333_residues
MINDQDKFRAYSKEYSQLEEVVTTFNRWKKLNNDIEEAQILLDDPDMKDMAAEEIAENKAEIENLEQHLQILLLPKDPND
EYNAFLEIRAGTGGDEAGIFAGDLYRMYSRYCESKRWRIEEMSANESEQGGYKEIIVKISGEGVYGQLKFESGGHRVQRV
PKTESQGRIHTSACTVAVMPELPESEMPEINPTDLRIDTYRSSGAGGQHVNTTDSAVRITHIPTGIVVECQDERSQHKNK
AKALAVLASRIVQVEQERQAAEQADTRRNLLGSGDRSDKIRTYNYPQGRVTDHRINLTVYRLDEVMNGKIDELIQPIITE
YQADQLAALSEQA

Specific function: Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA [H]

COG id: COG0216

COG function: function code J; Protein chain release factor A

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=288, Percent_Identity=47.9166666666667, Blast_Score=271, Evalue=5e-73,
Organism=Homo sapiens, GI34577120, Length=341, Percent_Identity=37.5366568914956, Blast_Score=222, Evalue=3e-58,
Organism=Homo sapiens, GI166795305, Length=193, Percent_Identity=48.7046632124352, Blast_Score=186, Evalue=2e-47,
Organism=Escherichia coli, GI1787462, Length=331, Percent_Identity=74.0181268882175, Blast_Score=510, Evalue=1e-146,
Organism=Escherichia coli, GI2367172, Length=313, Percent_Identity=39.9361022364217, Blast_Score=212, Evalue=2e-56,
Organism=Caenorhabditis elegans, GI17542784, Length=302, Percent_Identity=34.1059602649007, Blast_Score=181, Evalue=5e-46,
Organism=Saccharomyces cerevisiae, GI6321295, Length=315, Percent_Identity=42.5396825396825, Blast_Score=251, Evalue=8e-68,
Organism=Drosophila melanogaster, GI19921226, Length=337, Percent_Identity=40.0593471810089, Blast_Score=224, Evalue=6e-59,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR004373 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 37739; Mature: 37739

Theoretical pI: Translated: 4.66; Mature: 4.66

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MINDQDKFRAYSKEYSQLEEVVTTFNRWKKLNNDIEEAQILLDDPDMKDMAAEEIAENKA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCEEEECCCCHHHHHHHHHHHHHH
EIENLEQHLQILLLPKDPNDEYNAFLEIRAGTGGDEAGIFAGDLYRMYSRYCESKRWRIE
HHHHHHHCCEEEEEECCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCEEE
EMSANESEQGGYKEIIVKISGEGVYGQLKFESGGHRVQRVPKTESQGRIHTSACTVAVMP
CCCCCCCCCCCCEEEEEEEECCCEEEEEEECCCCCHHHCCCCCCCCCEEEECEEEEEECC
ELPESEMPEINPTDLRIDTYRSSGAGGQHVNTTDSAVRITHIPTGIVVECQDERSQHKNK
CCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCEEEEEECCCCEEEEECCHHHHHHHH
AKALAVLASRIVQVEQERQAAEQADTRRNLLGSGDRSDKIRTYNYPQGRVTDHRINLTVY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCEEEEEEEE
RLDEVMNGKIDELIQPIITEYQADQLAALSEQA
EECHHHCCCHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MINDQDKFRAYSKEYSQLEEVVTTFNRWKKLNNDIEEAQILLDDPDMKDMAAEEIAENKA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCEEEECCCCHHHHHHHHHHHHHH
EIENLEQHLQILLLPKDPNDEYNAFLEIRAGTGGDEAGIFAGDLYRMYSRYCESKRWRIE
HHHHHHHCCEEEEEECCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCEEE
EMSANESEQGGYKEIIVKISGEGVYGQLKFESGGHRVQRVPKTESQGRIHTSACTVAVMP
CCCCCCCCCCCCEEEEEEEECCCEEEEEEECCCCCHHHCCCCCCCCCEEEECEEEEEECC
ELPESEMPEINPTDLRIDTYRSSGAGGQHVNTTDSAVRITHIPTGIVVECQDERSQHKNK
CCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCEEEEEECCCCEEEEECCHHHHHHHH
AKALAVLASRIVQVEQERQAAEQADTRRNLLGSGDRSDKIRTYNYPQGRVTDHRINLTVY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCEEEEEEEE
RLDEVMNGKIDELIQPIITEYQADQLAALSEQA
EECHHHCCCHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA