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The map label for this gene is hisF [H]

Identifier: 126209481

GI number: 126209481

Start: 2254771

End: 2255403

Strand: Direct

Name: hisF [H]

Synonym: APL_2027

Alternate gene names: 126209481

Gene position: 2254771-2255403 (Clockwise)

Preceding gene: 126209480

Following gene: 126209482

Centisome position: 99.13

GC content: 49.61

Gene sequence:

>633_bases
GTGTTTTATGACATCACCGCTTCATCGGACGGTCGCACGGTGGATAAAAGCTGGGTGGAGCGTGTGGCACAGGTTATCGA
CATTCCGTTTTGCGTAGCGGGCGGGATTAAGACGATTGAAGACGCGGAAAAATTATTCGCTTTCGGGGCGGATAAAATCT
CAATAAATTCGCCCGCACTTGCCGATCCGAATTTAATTACCGCATTGGCAGATCGCTTTGGCGTGCAAGCTGTGGTAGTG
GGTATTGATAGTTGGTTTGAAAAAGAAACCGGCAAATATTGGGTTAATCAATACACCGGCGATGAAAAACGCACCCGCCA
AACTAACTGGCAGTTGCTCGACTGGGTGCAAGAGGTACAAAAACGTGGGGCAGGCGAAATCGTGCTAAATATGATGAACC
AAGACGGCGTGCGAAACGGCTACGATTTAACCCAACTTAAGCTCGTGCGTGAGGTGTGCAACGTGCCGTTAATCGCCTCA
GGCGGTGCAGGCGAAATGGTGCATTTCCGTGATGCTTTTACCCAAGCCAACGTGGACGGAGCATTGGCGGCGAGTGTGTT
CCACAAGCGGATTATTGAGATTGGCGAGTTGAAAGAATATCTTTACGCAGAGGGAGTTGAGATACGTCGTTAA

Upstream 100 bases:

>100_bases
TGGTCAGGTGGTCAAAGGCGTGCAGTTCCGCAACCACGAAATTATCGGCGACATCGTACCGCTTGCGAAACGCTATGCGG
AAGAAGGAGCGGACGAGTTA

Downstream 100 bases:

>100_bases
TACCTAGCACGAGCCGTGCTAGGTTCAATAATCCTAGCCGCTCTGCGGCTGTATATCTGAGAGAAAAAGAGCCACAGAGT
GGCTCAGATTATGTAACCCT

Product: imidazole glycerol phosphate synthase subunit hisF

Products: NA

Alternate protein names: IGP synthase cyclase subunit; IGP synthase subunit hisF; ImGP synthase subunit hisF; IGPS subunit hisF [H]

Number of amino acids: Translated: 210; Mature: 210

Protein sequence:

>210_residues
MFYDITASSDGRTVDKSWVERVAQVIDIPFCVAGGIKTIEDAEKLFAFGADKISINSPALADPNLITALADRFGVQAVVV
GIDSWFEKETGKYWVNQYTGDEKRTRQTNWQLLDWVQEVQKRGAGEIVLNMMNQDGVRNGYDLTQLKLVREVCNVPLIAS
GGAGEMVHFRDAFTQANVDGALAASVFHKRIIEIGELKEYLYAEGVEIRR

Sequences:

>Translated_210_residues
MFYDITASSDGRTVDKSWVERVAQVIDIPFCVAGGIKTIEDAEKLFAFGADKISINSPALADPNLITALADRFGVQAVVV
GIDSWFEKETGKYWVNQYTGDEKRTRQTNWQLLDWVQEVQKRGAGEIVLNMMNQDGVRNGYDLTQLKLVREVCNVPLIAS
GGAGEMVHFRDAFTQANVDGALAASVFHKRIIEIGELKEYLYAEGVEIRR
>Mature_210_residues
MFYDITASSDGRTVDKSWVERVAQVIDIPFCVAGGIKTIEDAEKLFAFGADKISINSPALADPNLITALADRFGVQAVVV
GIDSWFEKETGKYWVNQYTGDEKRTRQTNWQLLDWVQEVQKRGAGEIVLNMMNQDGVRNGYDLTQLKLVREVCNVPLIAS
GGAGEMVHFRDAFTQANVDGALAASVFHKRIIEIGELKEYLYAEGVEIRR

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit [H]

COG id: COG0107

COG function: function code E; Imidazoleglycerol-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family [H]

Homologues:

Organism=Escherichia coli, GI1788336, Length=209, Percent_Identity=80.8612440191388, Blast_Score=352, Evalue=9e-99,
Organism=Saccharomyces cerevisiae, GI6319725, Length=257, Percent_Identity=31.1284046692607, Blast_Score=101, Evalue=7e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR004651
- InterPro:   IPR011060 [H]

Pfam domain/function: PF00977 His_biosynth [H]

EC number: 4.1.3.-

Molecular weight: Translated: 23422; Mature: 23422

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFYDITASSDGRTVDKSWVERVAQVIDIPFCVAGGIKTIEDAEKLFAFGADKISINSPAL
CEEEEECCCCCCCCCHHHHHHHHHHHCCCHHHHCCCHHHHHHHHHHHHCCCEEECCCCCC
ADPNLITALADRFGVQAVVVGIDSWFEKETGKYWVNQYTGDEKRTRQTNWQLLDWVQEVQ
CCCHHHHHHHHHHCCEEEEEEHHHHHHHCCCCEEHHCCCCCHHHHHHCCHHHHHHHHHHH
KRGAGEIVLNMMNQDGVRNGYDLTQLKLVREVCNVPLIASGGAGEMVHFRDAFTQANVDG
HCCCCCEEHHHHHCCCCCCCCCHHHHHHHHHHHCCCEEECCCCCCEEHHHHHHHHCCCCH
ALAASVFHKRIIEIGELKEYLYAEGVEIRR
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MFYDITASSDGRTVDKSWVERVAQVIDIPFCVAGGIKTIEDAEKLFAFGADKISINSPAL
CEEEEECCCCCCCCCHHHHHHHHHHHCCCHHHHCCCHHHHHHHHHHHHCCCEEECCCCCC
ADPNLITALADRFGVQAVVVGIDSWFEKETGKYWVNQYTGDEKRTRQTNWQLLDWVQEVQ
CCCHHHHHHHHHHCCEEEEEEHHHHHHHCCCCEEHHCCCCCHHHHHHCCHHHHHHHHHHH
KRGAGEIVLNMMNQDGVRNGYDLTQLKLVREVCNVPLIASGGAGEMVHFRDAFTQANVDG
HCCCCCEEHHHHHCCCCCCCCCHHHHHHHHHHHCCCEEECCCCCCEEHHHHHHHHCCCCH
ALAASVFHKRIIEIGELKEYLYAEGVEIRR
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Lyases; Carbon-Nitrogen Lyases; Amidine-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA