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The map label for this gene is ispB [H]

Identifier: 126209449

GI number: 126209449

Start: 2221125

End: 2222120

Strand: Reverse

Name: ispB [H]

Synonym: APL_1995

Alternate gene names: 126209449

Gene position: 2222120-2221125 (Counterclockwise)

Preceding gene: 126209456

Following gene: 126209448

Centisome position: 97.7

GC content: 43.98

Gene sequence:

>996_bases
ATGACAACGCTATTAACACTCGAGCAAATTCAAACGCTTGCCAAACAAGATATGCAAGCGGTAGATGCTGAGATTCTCGC
TCAGCTTAACTCCGAAGTCGTTTTAATCAACCAACTAGGCAATTATATTATTGCCGGTGGCGGCAAACGTATTCGCCCGT
TAATTGCGGTGCTTGCCGCTAACGCATTAGGTTATCGTGGTAATAAACATGTTACTTGCGCCGCTTTTATCGAATTTGTG
CATACCGCCACTTTATTACACGATGATGTGGTGGACGAATCGGATATGCGTCGAGGTAGAGCGACGGCTAATGCAACCTT
CGGTAATGCCGCCAGCGTATTGGTCGGCGATTTCATTTATACGCGCTCATTCCAAATGATGACCGATGTCGATTCGCTTG
AAGTGTTAAAAGTGATGTCTGCCGCTACCAATGCGCTTGCCGAAGGTGAAGTTCAGCAACTGATGAATGTGAATGATCCG
AATACTACCGAAGCGAATTATATGCGTGTTATCTACAATAAAACCGCACGCTTATTTGAAGCGGCAACCCAATGTGTTGC
AATTGTGGCAGGTGTGGAAAAATCGGTTGAAACGGCGTTGAAAGATTACGGTTGTTATCTCGGTACTGCCTTCCAATTAA
TTGACGATATTTTAGATTACTCGGCAAAAGCGGAAAGTTTCGGCAAAAATGTCGGTGATGATTTCGTTGAAGGTAAACCG
ACTTTACCGTTGCTGCACGCTATGCACACTGCACAAGAGAAAGGTAATCAAGCTCAAGTGGATATAATTCGTTCAGCAAT
TGAAAATGGCGGGGAACGTGAACAAATTCCAGCCGTATTAGAAATTATGGCGGAACACCAATCACTTGAATATGCAATGA
CACGCGCTAAACAAGAAGCGCAAAAAGCGGTTGATGCCCTTGCCGTATTACCGGAAAGCGAATACAAACAAGCATTGATT
TCTCTTGCTTATTTATCGGTCGATCGTTCGTATTAA

Upstream 100 bases:

>100_bases
AAATTACATATAATTGGTTACTTATCACGATAATTTTTTGAAGCTCGCAATATTTGAGGTAGAATTATCGCCAAATTTTC
ACATTTAATATGAGAACGCA

Downstream 100 bases:

>100_bases
TAAGCGGTCAAATTTTTAGTCTTTTTTACAACTTTTATGGCAGAACAAAACCAACAACCTTTAGAACATAAACAAGCGTT
GCAACAAAAGAAAAAGCTCA

Product: octaprenyl-diphosphate synthase

Products: NA

Alternate protein names: All-trans-octaprenyl-diphosphate synthase; Octaprenyl pyrophosphate synthase; OPP synthase [H]

Number of amino acids: Translated: 331; Mature: 330

Protein sequence:

>331_residues
MTTLLTLEQIQTLAKQDMQAVDAEILAQLNSEVVLINQLGNYIIAGGGKRIRPLIAVLAANALGYRGNKHVTCAAFIEFV
HTATLLHDDVVDESDMRRGRATANATFGNAASVLVGDFIYTRSFQMMTDVDSLEVLKVMSAATNALAEGEVQQLMNVNDP
NTTEANYMRVIYNKTARLFEAATQCVAIVAGVEKSVETALKDYGCYLGTAFQLIDDILDYSAKAESFGKNVGDDFVEGKP
TLPLLHAMHTAQEKGNQAQVDIIRSAIENGGEREQIPAVLEIMAEHQSLEYAMTRAKQEAQKAVDALAVLPESEYKQALI
SLAYLSVDRSY

Sequences:

>Translated_331_residues
MTTLLTLEQIQTLAKQDMQAVDAEILAQLNSEVVLINQLGNYIIAGGGKRIRPLIAVLAANALGYRGNKHVTCAAFIEFV
HTATLLHDDVVDESDMRRGRATANATFGNAASVLVGDFIYTRSFQMMTDVDSLEVLKVMSAATNALAEGEVQQLMNVNDP
NTTEANYMRVIYNKTARLFEAATQCVAIVAGVEKSVETALKDYGCYLGTAFQLIDDILDYSAKAESFGKNVGDDFVEGKP
TLPLLHAMHTAQEKGNQAQVDIIRSAIENGGEREQIPAVLEIMAEHQSLEYAMTRAKQEAQKAVDALAVLPESEYKQALI
SLAYLSVDRSY
>Mature_330_residues
TTLLTLEQIQTLAKQDMQAVDAEILAQLNSEVVLINQLGNYIIAGGGKRIRPLIAVLAANALGYRGNKHVTCAAFIEFVH
TATLLHDDVVDESDMRRGRATANATFGNAASVLVGDFIYTRSFQMMTDVDSLEVLKVMSAATNALAEGEVQQLMNVNDPN
TTEANYMRVIYNKTARLFEAATQCVAIVAGVEKSVETALKDYGCYLGTAFQLIDDILDYSAKAESFGKNVGDDFVEGKPT
LPLLHAMHTAQEKGNQAQVDIIRSAIENGGEREQIPAVLEIMAEHQSLEYAMTRAKQEAQKAVDALAVLPESEYKQALIS
LAYLSVDRSY

Specific function: Supplies octaprenyl diphosphate, the precursor for the side chain of the isoprenoid quinones ubiquinone and menaquinone [H]

COG id: COG0142

COG function: function code H; Geranylgeranyl pyrophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FPP/GGPP synthase family [H]

Homologues:

Organism=Homo sapiens, GI50659086, Length=248, Percent_Identity=30.241935483871, Blast_Score=126, Evalue=3e-29,
Organism=Homo sapiens, GI4758430, Length=275, Percent_Identity=25.4545454545455, Blast_Score=82, Evalue=6e-16,
Organism=Homo sapiens, GI83700220, Length=275, Percent_Identity=25.4545454545455, Blast_Score=82, Evalue=6e-16,
Organism=Escherichia coli, GI1789578, Length=325, Percent_Identity=66.4615384615385, Blast_Score=436, Evalue=1e-124,
Organism=Escherichia coli, GI1786623, Length=298, Percent_Identity=29.1946308724832, Blast_Score=92, Evalue=5e-20,
Organism=Caenorhabditis elegans, GI17505681, Length=354, Percent_Identity=30.5084745762712, Blast_Score=142, Evalue=2e-34,
Organism=Saccharomyces cerevisiae, GI6319475, Length=296, Percent_Identity=30.0675675675676, Blast_Score=124, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6325188, Length=233, Percent_Identity=23.175965665236, Blast_Score=79, Evalue=8e-16,
Organism=Drosophila melanogaster, GI24651612, Length=302, Percent_Identity=31.4569536423841, Blast_Score=145, Evalue=3e-35,
Organism=Drosophila melanogaster, GI281365769, Length=275, Percent_Identity=24.3636363636364, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24660002, Length=275, Percent_Identity=24.3636363636364, Blast_Score=77, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000092
- InterPro:   IPR017446
- InterPro:   IPR008949 [H]

Pfam domain/function: PF00348 polyprenyl_synt [H]

EC number: =2.5.1.90 [H]

Molecular weight: Translated: 36102; Mature: 35971

Theoretical pI: Translated: 4.54; Mature: 4.54

Prosite motif: PS00723 POLYPRENYL_SYNTHET_1 ; PS00444 POLYPRENYL_SYNTHET_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTLLTLEQIQTLAKQDMQAVDAEILAQLNSEVVLINQLGNYIIAGGGKRIRPLIAVLAA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCEEEECCCCHHHHHHHHHHH
NALGYRGNKHVTCAAFIEFVHTATLLHDDVVDESDMRRGRATANATFGNAASVLVGDFIY
HHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCCCCCHHHHHHHHHHH
TRSFQMMTDVDSLEVLKVMSAATNALAEGEVQQLMNVNDPNTTEANYMRVIYNKTARLFE
HCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHH
AATQCVAIVAGVEKSVETALKDYGCYLGTAFQLIDDILDYSAKAESFGKNVGDDFVEGKP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCC
TLPLLHAMHTAQEKGNQAQVDIIRSAIENGGEREQIPAVLEIMAEHQSLEYAMTRAKQEA
CHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QKAVDALAVLPESEYKQALISLAYLSVDRSY
HHHHHHHHHCCCHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TTLLTLEQIQTLAKQDMQAVDAEILAQLNSEVVLINQLGNYIIAGGGKRIRPLIAVLAA
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCEEEECCCCHHHHHHHHHHH
NALGYRGNKHVTCAAFIEFVHTATLLHDDVVDESDMRRGRATANATFGNAASVLVGDFIY
HHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCCCCCHHHHHHHHHHH
TRSFQMMTDVDSLEVLKVMSAATNALAEGEVQQLMNVNDPNTTEANYMRVIYNKTARLFE
HCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHH
AATQCVAIVAGVEKSVETALKDYGCYLGTAFQLIDDILDYSAKAESFGKNVGDDFVEGKP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCC
TLPLLHAMHTAQEKGNQAQVDIIRSAIENGGEREQIPAVLEIMAEHQSLEYAMTRAKQEA
CHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QKAVDALAVLPESEYKQALISLAYLSVDRSY
HHHHHHHHHCCCHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800; 10675023 [H]